• Title/Summary/Keyword: markers

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Expression of a set of glial cell-specific markers in the Drosophila embryonic central nervous system

  • Ahn, Hui Jeong;Jeon, Sang-Hak;Kim, Sang Hee
    • BMB Reports
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    • v.47 no.6
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    • pp.354-359
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    • 2014
  • The types of glia in the central nervous system (CNS) of the Drosophila embryo include longitudinal glia (LG), cell body glia (CBG), and peripheral glia (PG). Transcription factors, such as glial cell missing and reverse polarity, are well-established general glial cell markers. Only a few glial cell-specific markers have been identified in the Drosophila embryonic CNS, thus far. In the present study, we employed the glial cell-specific markers for LG (vir-1/CG5453 and CG31235), CBG (fabp/CG6783 and CG11902), and PG (CG2310 and moody/CG4322), and comprehensively analyzed their expression patterns, during the embryonic CNS development. Our study validated the specificity of a set of glial markers, and further revealed their spatio-temporal expression patterns, which will aid in the understanding of the developmental lineage, and investigating their role in the development and homeostasis of the Drosophila CNS in vivo.

Reliability Assessment of Tubular Markers Used for Road Safety Facilities (도로안전시설용 시선유도봉의 신뢰성 평가)

  • Koo, Hyun-Jin;Yoon, Ye-Seok
    • Journal of Applied Reliability
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    • v.12 no.2
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    • pp.91-103
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    • 2012
  • Tubular markers for road safety facilities are used to lead the driver's sight line and separate the lanes on the road. Such tubular markers are usually installed on the road and frequently hit by vehicles, they are accordingly requested to assure the product durability. The traditional evaluation method of tubular markers include only quality tests of the material properties. However, most of consuming agencies in charge of road management at fields have proposed problems on long-term performance of the products hit by vehicles under various weather conditions. Therefore, the objectives of this study are to develop the reliability test methods and equipments to simulate the product failures of tubular markers due to vehicle collision and wheel compression and the delamination and discoloration of reflection sheets attached on the surface of the products under high and low temperatures.

Combined Genome Mapping of RFLP-AFLP-SSR in Pepper

  • Lee, Je Min;Kim, Byung-Dong
    • Genomics & Informatics
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    • v.1 no.2
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    • pp.108-112
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    • 2003
  • We have constructed a molecular linkage map of pepper (Capsicum spp.) in an interspecific $F_2$ population of 107 plants with 320 RFLP, 136 AFLP, and 46 SSR markers. The resulting linkage map consists of 15 linkage groups covering 1,720 cM with an average map distance of 3.7 cM between framework markers. Most RFLP markers ($80\%$) were pepper-derived clones and these markers were evenly distributed all over the genome. Genes for defense and biosynthesis of carotenoids and capsaicinoids were mapped on this linkage map. By using 30 primer combinations, AFLP markers were generated in the $F_2$ population. For development of SSR markers in Capsicum, microsatellites were isolated from two small-insert genomic libraries and the GenBank database. This combined map provides a starting point for high-resolution QTL analysis, gene isolation, and molecular breeding.

Research Progress in Potential Urinary Markers for the Early Detection, Diagnosis and Follow-up of Human Bladder Cancer

  • Wang, Hai-Feng;Wang, Jian-Song
    • Asian Pacific Journal of Cancer Prevention
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    • v.13 no.5
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    • pp.1723-1726
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    • 2012
  • Objective: To summarize and evaluate various urinary markers for early detection, diagnosis and follow-up of human bladder cancer. Methods: A MEDLINE and PUBMED search of the latest literature on urinary markers for bladder cancer was performed. We reviewed these published reports and made a critical analysis. Results: Most urinary markers tend to be less specific than cytology, yielding more false-positive results, but demonstrating an advantage in terms of sensitivity, especially for detecting low grade, superficial tumors. Some tumor markers appear to be good candidates for early detection, diagnosis, and follow-up of human bladder cancer. Conclusion: A number of urinary markers are currently available that appear to be a applicable for clinical detection, diagnosis, and follow-up of bladder cancer. However, further studies are required to determine their accuracy and widespread applicability.

Genetically Independent Tetranucleotide to Hexanucleotide Core Motif SSR Markers for Identifying Lentinula edodes Cultivars

  • Saito, Teruaki;Sakuta, Genki;Kobayashi, Hitoshi;Ouchi, Kenji;Inatomi, Satoshi
    • Mycobiology
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    • v.47 no.4
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    • pp.466-472
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    • 2019
  • For the purpose of protecting the rights of Lentinula edodes breeders, we developed a new simple sequence repeat (SSR) marker set consisting only of genetically independent tetranucleotide or longer core motifs. Using available genome sequences for five L. edodes strains, we designed primers for 13 SSR markers that amplified polymorphic sequences in 20 L. edodes cultivars. We evaluated the independence of every possible marker pair based on genotype data. Consequently, eight genetically independent markers were selected. The polymorphic information content values of the markers ranged from 0.269 to 0.764, with an average of 0.409. The markers could distinguish among 20 L. edodes cultivars and produced highly repeatable and reproducible results. The markers developed in this study will enable the precise identification of L. edodes cultivars, and may be useful for protecting breeders' rights.

Genome Research on Peach and Pear

  • Hayashi, Tateki;Yamamoto, Toshiya
    • Journal of Plant Biotechnology
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    • v.4 no.2
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    • pp.45-52
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    • 2002
  • A lot of SSRs (simple sequence repeats) in peach and pear from enriched genomic libraries and in peach from a cDNA library were developed. These SSRs were applied to other related species, giving phenograms of 52 Prunes and 60 pear accessions. Apple SSRs could also be successfully used in Pyrus spp. Thirteen morphological traits were characterized on the basis of the linkage map obtained from an $F_2$ population of peach. This map was compiled with those morphological markers and 83 DNA markers, including SSR markers used as anchor loci, to compare different peach maps. Molecular markers tightly linked to new root-knot nematode resistance genes were also found. A linkage map including disease related genes, pear scab resistance and black spot susceptibility, in the Japanese pear Kinchaku were constructed using 118 RAPD markers. Another linkage map, of the European pear Bartlett, was also constructed with 226 markers, including 49 SSRs from pear, apple, peach and cherry. Maps of other Japanese pear cultivars, i.e., Kousui and Housui, were also constructed. These maps were the first results of pear species.

Clinical significance of sonographic soft markers: A review

  • Kim, Mi Sun;Kang, Sukho;Cho, Hee Young
    • Journal of Genetic Medicine
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    • v.15 no.1
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    • pp.1-7
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    • 2018
  • Sonographic findings with little or no pathological significance, known as soft markers, are often found in aneuploidy fetuses. After normal screening for the aneuploidy in first trimester, there are no uniform recommendations regarding when to disregard or put on clinical significance in isolated soft markers. Associations between some soft markers and adverse pregnancy outcomes including intrauterine fetal death, preterm birth, fetal growth restriction, and congenital infection have been reported in euploidy fetuses. The present article aims to review recent literatures about the clinical significance of soft markers after normal first trimester combined screening or noninvasive prenatal testing, and propose a simple clinical summary for management of specific soft markers in pregnancies.

The Variable Acquisition of Discourse Marker Use in Korean American Speakers of English

  • Lee, Hi-Kyoung
    • English Language & Literature Teaching
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    • v.11 no.2
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    • pp.1-18
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    • 2005
  • This study is a preliminary investigation of the nature of discourse marker acquisition in Korean American speakers of English. Discourse markers are of interest because they are not an aspect of language taught through formal instruction either to native or non-native speakers. Therefore, discourse marker use serves as indirect evidence of face-to-face interaction with native speakers and an indicator of integration. In this light, the present study examines the presence of discourse markers in Korean Americans. The markers chosen for analysis were you know, like, and I mean. The data consist of spontaneous speech elicited from interviews. Sociolinguistic variables such as age, sex, and generation (i.e., $1^{st}$, 1.5, $2^{nd}$) were examined. Results show that there appears to be interaction between the variables and discourse marker use. While all speakers showed variable acquisition of markers, younger, female, and 1.5 generation speakers were found to use discourse markers more than other speakers. Although discourse marker use is optional and thus not a linguistic feature that must be necessarily acquired, it is clear that use is pervasive and acquired differentially by English speakers irrespective of whether they are native or not.

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Positioning sensor system for mobile robots using magnetic markers (마그네틱 마커를 이용하는 이동로봇을 위한 위치인식 센서 시스템)

  • Kim, Eui-Sun;Kim, Won-Ho
    • Journal of Sensor Science and Technology
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    • v.19 no.3
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    • pp.221-229
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    • 2010
  • In recent studies, many methods have been studied for mobile robot using magnetic markers on its pathway. This is not influenced by the weather conditions, and makes possible to develop controller with low level processors and simple algorithms. However, the interval between magnets is restricted by the magnetic field intensity and it is impossible to get road information ahead. This paper suggests a method of widening markers and expressing the road information ahead using magnetic markers, and explains a sensor arrangement considering suggested methods. Also, magnetic field analysis was done to investigate the effects of widening magnetic markers with various environments. A small mobile robot was made to figure out the performance of suggested methods, and driving experiments were performed on the straight and curved road with magnetic markers. The results show that the robot moved the prearranged pathway with 0.5 cm lateral displacements and stopped at a stop line using magnetic information on the road.

Multi-Marker Augmented Reality System using Marker-Based Tracking with Vuforia

  • Yun, Hyun-Noh;Kim, Gi-Seong;Moon, Nammee
    • Journal of the Korea Society of Computer and Information
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    • v.24 no.2
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    • pp.119-126
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    • 2019
  • As interest in augmented reality has increased recently, attempts have been made to incorporate augmented reality into various fields. In implementing augmented reality, the method by which markers are used is to extract feature points of markers to recognize 3D coordinates and, in some cases, it is necessary to recognize multiple markers simultaneously. Therefore, this paper proposes optimization methods for recognising multiple markers at the same time. Unity 3D and augmented reality library Vuforia are used to implement the experimental environment. The augmented reality program produced was implemented in an application form and tested using a mobile camera. We looked for optimization methods for manufacturing markers directly and for recognizing multiple markers through changes in the experimental environment. The results of the experiment can provide a higher recognition rate in an environment where multiple marker recognition is required later.