• Journal of Microbiology and Biotechnology
• /
• v.23 no.4
• /
• pp.437-443
• /
• 2013

The diversity of culturable non-actinobacterial (NA) bacteria associated with four species of South China Sea gorgonians was investigated using culture-dependent methods followed by analysis of the bacterial 16S rDNA sequence. A total of 76 bacterial isolates were recovered and identified, which belonged to 21 species of 7 genera, and Bacillus was the most diverse genus. Fifty-one percent of the 76 isolates displayed antibacterial activities, and most of them belonged to the Bacillus genus. From the culture broth of gorgonian-associated Bacillus methylotrophicus SCSGAB0092 isolated from gorgonian Melitodes squamata, 11 antimicrobial lipopeptides including seven surfactins and four iturins were obtained. These results imply that Bacillus strains associated with gorgonians play roles in coral defense mechanisms through producing antimicrobial substances. This study, for the first time, compares the diversity of culturable NA bacterial communities among four species of South China Sea gorgonians and investigates the secondary metabolites of gorgonian-associated B. methylotrophicus SCSGAB0092.

• Journal of Marine Life Science
• /
• v.8 no.1
• /
• pp.56-67
• /
• 2023

Marine macroalgae are important in coastal ecosystems and interact with marine microorganisms. In this study, we isolated fungi from seven types of marine macroalgae including Cladophora sp., Gloiopeltis furcate, Gracilariopsis chorda, Hydroclathrus clathratus, Prionitis crispata, Sargassum micracanthum, and Ulva lactuca collected in Korea. Morphological and phylogenetic analyses identified the isolates as four Aspergillus spp. (A. fumigatus, A. sydowii, A. tamarii, and A. terreus), three Penicillium spp. (P. crustosum, P. jejuense, and P. rubens), and Cladosporium tenuissimum. Among them, A. fumigatus TOP-U2, A. tamarii SH-Sw5, and A. terreus GJ-Gf2 strains showed the activities of all enzymes examined (amylase, chitinase, lipase, and protease). Based on the enzymatic index (EI) values in solid media, A. terreus GJ-Gf2 and C. tenuissimum UL-Pr1 exhibited the highest amylase and lipase activities, respectively. Chitinolytic activity was only observed in A. terreus GJ-Gf2, A. tamarii SH-Sw5, and A. fumigatus TOP-U2. Penicillium crustosum UL-Cl2 and C. tenuissimum UL-Pr1 showed the highest protease activities. To the best of our knowledge, this is the first report of lipolytic and proteolytic activities in a marine-derived C. tenuissimum strain. Overall, the fungal strains isolated from the marine macroalgae in this study actively produced industrially important enzymes.

• Ocean and Polar Research
• /
• v.26 no.2
• /
• pp.361-365
• /
• 2004

Deep-sea Resources Research Center (DRRC) has been using a free-fall grab (FFG) for the resource evaluation of polymetallic nodules in the KODOS (Korea Deep Ocean Study) area, NE Pacific, since 1992. A FFG model (Model 4200, Benthos) adapting one glass buoy system (diameter 17 inch, net buoyance 25.4 kg) has been used until 1993. Since then, DRRC has carried out a study to increase the recovery rate of FFG and it was found that a revised system adapting an additional buoy (diameter 10 inch, net buoyance 4.5 kg) gives improved results. The nodule sampling tests were performed using two different models for the same study area and the resulting recovery rates were compared. The result shows no distinct difference in recovery time between two models, but average recovery rate of the revised model was increased to 99.44% from 96.06% of the original model.

• Journal of Species Research
• /
• v.12 no.2
• /
• pp.158-164
• /
• 2023

In March 2021, marine sediment from East Sea samples were suspended in a 2% NaCl solution, and serial dilution was performed in fresh marine and Reasoner's 2A agar. Isolated bacterial strains were identified based on 16S rRNA gene sequences, and showed at least 98.7% sequence similarity with previously reported bacterial species. Finally, seven bacterial strains which were validly published but not reported in Korea, were obtained. These isolates were allocated to the orders Bacillales and Flavobacteriales. The three Flavobacteriales strains are classified into the family Flavobacteriaceae. The other four Bacillales belong to the families Bacillaceae and Paenibacillaceae. The seven unrecorded bacterial strains in this study are classified into seven different genera, which are assigned to Mesobacillus, Paenibacillus, Gramella, Gillisia, Arenibacter, Fictibacillus, and Brevibacillus. During the investigation, the possibility of excavation of various unrecorded species in domestic marine sediment was confirmed. Gram-staining, cell morphology, physiological and basic biochemical characteristics, and phylogenetic analysis were performed in this study and provided in the description of each strain.

• Journal of Microbiology and Biotechnology
• /
• v.27 no.6
• /
• pp.1078-1089
• /
• 2017

Biofilm formation of Staphylococcus aureus is one of its mechanisms of drug resistance. Anti-biofilm screening of 106 compounds from marine-derived fungi displayed that 12 compounds inhibited S. aureus biofilm formation by >50% at the concentration of $100{\mu}g/ml$, and only secalonic acid D (SAD) and B inhibited by >90% at $6.25{\mu}g/ml$ without inhibiting cell growth after 24-h incubation. Meanwhile, it was found that the double bond between C-1 and C-10 of citrinin derivatives and the C-C connection position of two chromone monomers may be important for their anti-biofilm activities. Moreover, SAD slightly facilitated biofilm eradication and influenced its architecture. Furthermore, SAD slowed the cell growth rate in the preceding 18-h incubation and differentially regulated transcriptional expression of several genes, such as agr, isaA, icaA, and icaD, associated with biofilm formation in planktonic and biofilm cells, which may be the reason for the anti-biofilm activity of SAD. Finally, SAD acted synergistically against S. aureus growth and biofilm formation with other antibiotics. These findings indicated that various natural products from marine-derived fungi, such as SAD, could be used as a potential biofilm inhibitor against S. aureus.

• Ocean and Polar Research
• /
• v.26 no.2
• /
• pp.377-384
• /
• 2004

Annual variations of inorganic nutrients such as nitrate(+nitrite), phosphate and silicate in association with thermocline were investigated in the upper 200 m of the water column at KODES Long-term Monitoring (KOMO) station in the northeast equatorial Pacific from 1995 to 2002. Global climatic disturbances such as El Nino and La Nina, should have affected KODES area during the study period. In 1995-97 and 2000-2002, a thermocline where temperatures rapidly decrease with depth, was formed at 50-70 m water depth. Nutrient depletion, specially for nitrate and phosphate, was extended down to approximately 50 m depth, which coincided with the surface mixed layer depth. In 1998 and 1999, however a very fluctuating thermocline was observed at 20-100 m water depth. In the photic zone (up to 100 m depth), depth integration of nitrate, phosphate and silicate ranged from 2.02 to $23.14\;gN/m^2$, from 0.87 to $4.05\;gP/m^2$ and from 35.67 to $176.21\;gSi/m^2$, respectively. As a result of changes in the water column structures, nutrient concentrations also showed fluctuation parallel to the changes of thermocline in the study area.

• Proceedings of the Korean Aquaculture Society Conference
• /
• 2006.05a
• /
• pp.340-341
• /
• 2006

• Economic and Environmental Geology
• /
• v.43 no.5
• /
• pp.517-521
• /
• 2010

• Journal of Fisheries and Marine Sciences Education
• /
• v.24 no.3
• /
• pp.378-386
• /
• 2012

The objective of this study was to search the attributes and calculate the weights among the attributes considered to choose the best thing of various tourism resources focused on marine ranch of Chagwido in Jeju region. Through this analysis, this research provides the information for establishing development plan of Chagwido. To achieve this goal, this study analyzed the priority of experience tourism through the experts' survey with AHP methodology. The result showed that ecological experience (30.6%), fishing activities (23.1%), release of fish seed (14.9%) and culture experience (14.9%) among various tourism resources in marin ranch of Chagwido were in order of importance. Thus ecological experiences, activities related to fishing facilities and programs should be introduced in order to develop the marine ranch of Chagwido.

• Fisheries and Aquatic Sciences
• /
• v.24 no.11
• /
• pp.341-350
• /
• 2021

The energy production of offshore wind farms plays an important role in expanding renewable energy. However, the development of offshore wind farms faces many challenges due to its incompatibility with marine environments and its social acceptability among the local community. In this study, we reviewed the marine environmental impact assessment status of offshore wind farm development projects for 2012-2019 in South Korea. A total of nine projects were selected for this study, all of which experienced considerable conflict with local fisheries resources. To appropriately respond to the underlying challenges faced by offshore wind farm development and in order to better support decision-making for future impact assessment, our findings identified: i) a need for adequate preliminary investigation and technical examination of fisheries resources; ii) a need to assess and estimate the impact of underwater noise, vibration, and electromagnetic waves on fisheries resources during wind farm construction and operation; and iii) a need for a bottom-up approach that allows for communication with local stakeholders and policy-makers to guarantee the local acceptability of the development.