Journal of Species Research

The National Institute of Biological Resources (국립생물자원관)
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A report of seven unrecorded bacterial species in Korea, isolated from marine sediment

  • Chi Young Hwang (Department of Bioengineering and Nano-Bioengineering, Incheon National University) ;
  • Eui-Sang Cho (Department of Bioengineering and Nano-Bioengineering, Incheon National University) ;
  • Dong-Hyun Jung (Microorganism Resources Division, National Institute of Biological Resources) ;
  • Ki-Eun Lee (Microorganism Resources Division, National Institute of Biological Resources) ;
  • In-Tae Cha (Microorganism Resources Division, National Institute of Biological Resources) ;
  • Won-Jae Chi (Microorganism Resources Division, National Institute of Biological Resources) ;
  • Myung-Ji Seo (Department of Bioengineering and Nano-Bioengineering, Incheon National University)
  • Received : 2022.12.30
  • Accepted : 2023.02.09
  • Published : 2023.05.31
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Abstract

In March 2021, marine sediment from East Sea samples were suspended in a 2% NaCl solution, and serial dilution was performed in fresh marine and Reasoner's 2A agar. Isolated bacterial strains were identified based on 16S rRNA gene sequences, and showed at least 98.7% sequence similarity with previously reported bacterial species. Finally, seven bacterial strains which were validly published but not reported in Korea, were obtained. These isolates were allocated to the orders Bacillales and Flavobacteriales. The three Flavobacteriales strains are classified into the family Flavobacteriaceae. The other four Bacillales belong to the families Bacillaceae and Paenibacillaceae. The seven unrecorded bacterial strains in this study are classified into seven different genera, which are assigned to Mesobacillus, Paenibacillus, Gramella, Gillisia, Arenibacter, Fictibacillus, and Brevibacillus. During the investigation, the possibility of excavation of various unrecorded species in domestic marine sediment was confirmed. Gram-staining, cell morphology, physiological and basic biochemical characteristics, and phylogenetic analysis were performed in this study and provided in the description of each strain.

Keywords

Acknowledgement

This work was supported by grants from the National Institute of Biological Resources funded by the Ministry of Environment(No. NIBR202203112), Republic of Korea.

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