• Title/Summary/Keyword: marine product

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Identification and Functional Analysis of the putAP Genes Encoding Vibrio vulnificus Proline Dehydrogenase and Proline Permease

  • Kim, Hye-Jin;Lee, Jeong-Hyun;Rhee, Jee-Eun;Jeong, Hye-Sook;Choi, Hyun-Kyung;Chung, Hee-Jong;Ryu, Sang-Ryeol;Choi, Sang-Ho
    • Journal of Microbiology and Biotechnology
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    • v.12 no.2
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    • pp.318-326
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    • 2002
  • The pathogenic marine bacterium Vibrio vulnificus is the causative agent of food-borne diseases such as life-threatening septicemia. To better understand this organism's strategies to survive osmotic stress, a mutant that was more sensitive to high osmolarity was screened from a library of mutants constructed by a random transposon mutagenesis. By a transposon-tagging method, putAP genes encoding a proline dehydrogenase and a proline permease were identified and cloned from V. vulnificus. The amino acid sequences deduced from nucleotide sequences of putAP from V. vulnificus were 38 to $59\%$ similar to those of PutA and PutP reported from other Enterobacteriaceae. Functions of putAP genes were assessed by the construction of mutants, whose putAP genes were inactivated by allelic exchanges. When proline as the sole carbon or nitrogen source was used, the putA mutant was not able to grow to the substantial level, revealing the proline dehydrogenase is the only enzyme for metabolic conversion of proline into other amino acids. Although the growth rate of the putP mutant on proline as the sole carbon or nitrogen source was significantly reduced, the mutant still grew. This indicated that at least one more proline permease is produced by V. vulnificus. The putP mutant decreased approximately $2-log_10$ CFU/ml after a hyperosmotic challenge, while the parent strain decreased approximately $l-log_10$ CFU/ml. This result suggests that the gene product of putP contributes to the osmotic tolerance of V. vulnificus.

Quality Characteristics of Fried Fish Paste of Alaska Pollack Meat Paste Added with Propolis (프로폴리스 첨가 명태 연육 튀김어묵의 품질 특성)

  • Kim, Gwang-Woo;Kim, Ga-Hyeon;Kim, Jeong-Sik;An, Hyo-Yeong;Hu, Gil-Won;Park, In-Suk;Kim, Ok-Seon;Cho, Soon-Yeong
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.37 no.4
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    • pp.485-489
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    • 2008
  • In this study, the fried fish paste was prepared from Alaska pollack meat paste added with propolis. The quality characteristics were analyzed by peroxide value, gel strength, color, viable cell count and sensory evaluation. The fried fish paste product had lower peroxide value and viable cell count after frying compared to the one without propolis. The antioxidant and antispoiling ability of propolis in fried fish paste increased with content of propolis. Gel strength increased with increasing addition of propolis. L-value decreased but a-value and b-value increased with the addition of propolis. In sensory evaluation, 0.17% propolis had the best score in overall acceptability. These results indicate that the fish paste could be prepared by adding the propolis for high quality and functionality. Consequently, propolis can be applied as a food preservative or additive.

Estimation of Concentrations in Foods and Their Daily Intake for Sorbic Acid in Korea (식품 중 소르빈산의 사용실태 및 섭취량에 관한 연구)

  • Kim, Hee-Yun;Jang, Young-Mi;Yoon, Hae-Jung;Lee, Chang-Hee;Park, Sung-Kwan;Park, Sun-Young;Shin, Il-Shick;Lee, Jong-Ok;Chin, Myung-Shik;Lee, Chul-Won
    • Korean Journal of Food Science and Technology
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    • v.34 no.6
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    • pp.1103-1109
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    • 2002
  • Mean concentration and daily intake of sorbic acid were estimated through the analysis of food samples obtained from Korea FDA and food sanitation inspection agency during $1999{\sim}2000$. Total number of samples were 14,129, among which the number of detected samples were 2,561 (detection rate was 18.13%). Contribution rate to total estimated daily intake (%) of sorbic acid in the categorized food were the highest in processed fish product. Total estimated daily intake $({\Sigma}EDI)$ for age groups were the highest in $30{\sim}49$ years old and ranged from 3.42 to 9.25 mg/person/day. Estimation of average food intake for each food item per person per day was based on data in national nutrition survey and national food disapperance data in 1998. Total estimated daily intake of sorbic acid was 20.40 mg per body weight of 55 kg. This value was 1.48% of acceptable daily intake evaluated by FAO/WHO and 33.03% of theoretical maximum daily intake.

Effects of Fisheries Technological Innovation on Growth per Capita across OECD Countries (수산부문 기술혁신이 OECD 회원국의 성장률에 미친 효과)

  • Lee, Yoonsuk;Chang, Jae Bong
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.18 no.4
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    • pp.349-357
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    • 2017
  • The environmental problems affecting marine resources and slow growth in the fisheries industry is causing many countries to look for alternative inputs that can boost the fisheries sector. This study focuses on the effects of technological innovation in the fisheries industry on the gross domestic product (GDP) per capita across Organization for Economic Cooperation and Development (OECD) countries. Using a panel dataset, this study attempts to estimate the different effects of technological innovations in the fisheries industry from country to country using the differences-in-differences (DiD) method. After the DiD method, the Granger causality test is applied to determine the interactive relations between economic growth and the selected variables associated with technological innovation in the fisheries industry, such as government spending on fisheries R&D, the number of patents in fisheries, and employment. The results obtained from the DiD estimation show that government spending on fisheries R&D, fisheries technology development, and fisheries employment positively influences the GDP per capita across OECD counties. From the causality test, we found different bi-directional causal relationships between the GDP per capita and (spending) on fisheries technology development across countries.

Effect of Thermally Grown TiO2 Interlayer on Chlorine Evolution Efficiency and Durability of Ti/RuO2-PdO-TiO2 Electrodes (열산화법으로 생성된 TiO2 중간보호층이 Ti/RuO2-PdO-TiO2전극의 염소발생 효율 및 내구성에 미치는 영향)

  • Park, Da Jung;Choi, Sung Mook;Lee, Kyu Hwan
    • Journal of the Korean institute of surface engineering
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    • v.51 no.4
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    • pp.207-213
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    • 2018
  • Not only efficiency of chlorine evolution reaction (CER) but also durability namely service life is very important property in dimensional stable anode for Ballast Water Management System (BWMS) for marine ships. Many researchers have been focused on improving efficiency of CER by controlling composition, phase and surface area for a long time, but the efforts to increase durability was relatively small. In this study, we have investigated the effect of $TiO_2$ protective interlayers on efficiency and durability of DSA electrodes. $TiO_2$ protective interlayers were prepared by thermal oxidation at 500, 600 and $700^{\circ}C$ on Ti substrate. And then the DSA electrodes consisting of $Ti/RuO_2-PdO-TiO_2$ were prepared by thermal decomposition method on $TiO_2$ interlayers. The efficiencies of CER of DSA electrodes without $TiO_2$ interlayer and with $TiO_2$ interlayer grown at 500, 600 and $700^{\circ}C$ were 94.19, 94.45, 84.60 and 76.75% respectively. On the otherhand, durabilities were 30, 55, 90 and 65 hours respectively. In terms of industrial aspect, the performance of DSA is considered high efficiency and durability which can correspond to total production of chlorine. If we considered the performance index of DSA as the product of efficiency and durability, performance indices could be recalculated as 28.26, 50.85, 76.14 and 49.89 respectively. As the thermal oxidation temperature increasing, life time were increased remarkerbly, while efficiency of CER was decreased slightly. As a result, DSA electrode with $TiO_2$ interlayer grown at $600^{\circ}C$ has shown about 2.7 times performace of original DSA electrode without $TiO_2$ interlayer.

Correlation between Characteristics of SOD in Coastal Sewage and Predictive Factor (연안 저질 SOD의 특성과 유발 영향인자에 대한 상관관계)

  • Kim, Beom-Geun;Khirul, Md Akhte;Kwon, Sung-Hyun;Cho, Dae-Chul
    • Korean Journal of Environment and Ecology
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    • v.33 no.5
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    • pp.596-604
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    • 2019
  • This study conducted a sediment culture experiment to investigate the effects of sediment oxygen demand (SOD) and environmental factors on sediment and water quality. We installed a leaching tank in the laboratory, cultured it for 20 days, and analyzed the relationship between P and Fe in the sediment. As a result, the dissolved oxygen of the water layer decreased with time, while the oxidation-reduction potential of the sediment progressed in the negative direction to form an anaerobic reducing environment. The SOD was measured to be 0.05 mg/g at the initial stage of cultivation and increased to 0.09 mg/g on the 20th day, indicating the tendency of increasing consumption of oxygen by the sediment. The change is likely to have caused by oxygen consumption from biological-SOD, which is the decomposition of organic matter accumulated on the sediment surface due to the increase of chl-a, and chemical-SOD consumed when the metal-reducing product produced by the reduction reaction is reoxidized. The correlation between SOD and causality for sediment-extracted sediments was positive for Ex-P and Org-P and negative for Fe-P. The analysis of the microbial community in the sediment on the 20th day showed that anaerobic iron-reducing bacteria (FeRB) were the dominant species. Therefore, when the phosphate bonded to the iron oxide is separated by the reduction reaction, the phosphate is eluted into the water to increase the primary productivity. The reduced substance is reoxidized and contributes to the oxygen consumption of the sediment. The results of this study would be useful as the reference information to improve oxygen resin.

The Age of the Earth: Reappraisal (지구의 나이: 재평가)

  • Kwon, Sung-Tack
    • The Journal of the Petrological Society of Korea
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    • v.23 no.3
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    • pp.273-277
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    • 2014
  • This paper presents a brief historical review of various attempts to estimate the age of the Earth, and reappraises the study of Patterson (1956) which revealed for the first time that the age of the Earth is $4550{\pm}70Ma$ by measuring Pb isotope ratios of several meteorites and a marine sediment. The standard model for the planetary formation of early solar system is: formation of solid particles condensed from the cooling of hot nebular gas -> formation of planet-sized bodies by accretion of those solid particles. The Moon is supposed to have formed from the accretion of the relicts produced by the collision of proto-Earth with Mars-sized body. It is not easy to pinpoint the age of the Earth, considering the series of events related to the formation of the Earth. So, I propose that the collision age as that of the Earth, since the present status of the Earth is thought to be the direct product of the collision. According to the previous studies, the collision age can be broadly constrained between the age ($4567.30{\pm}0.16Ma$) of the earliest condensates (CAI, calcium-aluminum rich inclusion) of the nebula gas, i.e., the age of the solar system, and the oldest age ($4,456{\pm}40Ma$) among rocks and minerals of the Earth and the Moon. We need more precise estimation of the collision age, since it is important in estimating time scale for the formation of planet-size body and in revealing thermal evolution of magma oceans of the Earth and the Moon presumably developed right after the collision.

Comparison of Nutritional Composition of Noodle Products in Korean Key Foods (국내 주요 식품(Key foods) 중 면류 제품의 영양성분 함량 비교)

  • Cha, Seung-Hyeon;Han, In-Beom;Park, Woo-Hyun;Park, Sang-Beom;Bak, Se-Lim;Kim, Byung Hee;Yoon, Sung-Won;Kim, In Hwan;Chun, Jiyeon;Shin, Jung-Ah;Kim, Younghwa;Shin, Eui-Cheol;Seo, Dongwon;Lee, Sam-pin;Sung, Jeehye;Kim, So-Jung;Lee, Jun-Soo;Jang, Keum-Il
    • The Korean Journal of Food And Nutrition
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    • v.34 no.5
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    • pp.449-457
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    • 2021
  • This study analyzed the nutritional composition (proximate composition, total dietary fiber, calories, minerals, fatty acids, and amino acids) of 10 noodle products (tteok ramyun, jjamppong ramyun, kimchi ramyun, instant udon, cup ramyun, jajangmyun, bibimmyun, cream spaghetti, ssalguksu, and milmyun), which account for 85% of the cumulative intake of one or more key nutrients, using data from the 7th Korea National Health and Nutrition Examination Survey. The moisture contents of bibimmyun, jajangmyun, and cream spaghetti were lower than those of the other noodle products, whereas the crude fat, crude protein, carbohydrate, and calorie contents were the highest. Cream spaghetti had the highest mineral, fatty acid, and amino acid contents, followed by bibimmyun and jajangmyun. Ssalguksu had the lowest contents of most nutrients. These data could be used to populate a food composition database, which can provide consumers with the nutritional information about frequently consumed noodle products.

Preparation and Quality Characteristics of Different Mackerel Scomber japonicas-based Processed Products as Senior-friendly Seafoods (물성을 달리한 고등어(Scomber japonicus) 활용 고령친화식품의 제조 및 품질특성)

  • Jang, Mi-Soon;Oh, Jae-Young;Kim, Poong-Ho;Park, Sun Young;Kim, Ye Youl;Kang, Sang In;Kim, Jin-Soo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.54 no.5
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    • pp.703-713
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    • 2021
  • Mackerel Scomber japonicus is an excellent source of vitamins (A, D and B12), omega-3 fatty acids, dietary protein and minerals. This study was conducted to prepare mackerel-based processed seafood products such as grilled product with super-heated (GM), tang (TM) and salad with sweet potato (SSM) using mackerel as senior-friendly seafoods and to examine their quality characteristics. The hardness of GM, TM and SSM were 240.3×1.03, 21.7×1.03 and 7.4×1.03 N/m2, respectively. The viscosity of SSM was 25,450 m·Pas. The nutritional content of mackerel-processed products was within the senior-friendly standard specifications for protein, vitamin C, vitamin B2 (riboflavin) and vitamin B3 (niacin) ing GM, protein, vitamin B3 and calcium for TM, and protein, vitamin D, vitamin B2 and vitamin B3 in SSM. All the mackerel-processed products were safe as senior-friendly seafoods, since digestibility rates were 81.5% for GM, 87.9% for TM and 93.5% for SSM. The physical, nutritional and the sanitation results indicated that senior-friendly seafoods classified of the mackerel-processed products was step 1 for GM, step 2 for TM and step 3 for SSM according to the Korean Food Code and Korean Indusrty Standards.

A Study on the Promoting Policies for Cruise Industry in Yeosu Port (여수항 크루즈산업 육성 방안에 관한 연구)

  • Maowei Chen;Hyangsook Lee;Kyongjun Yun
    • Journal of Korea Port Economic Association
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    • v.39 no.1
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    • pp.1-16
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    • 2023
  • As the transfer of cargo functions to Gwangyang Port is completed, Yeosu Port has established a vision to become a hub for maritime tourism in Korea. Revitalization of the marine tourism industry through cruise development is recognized as a major strategy. Major domestic cruise tourism ports (Busan Port, Jeju Port, Incheon Port) have established a basic plan for fostering port cruises in response to the "Basic Plan for the Promotion of the Cruise Industry" established in 2016. However, the plan for Yeosu Port is insufficient. The cruise tourism industry, which is in recession due to Corona 19, is expected to recover due to with-Corona and Post-Corona. Therefore, Yeosu Port needs to take the lead in promoting the cruise industry to prepare for this. Accordingly, this study intends to derive evaluation items for the fostering of the Yeosu Port cruise industry through prior research and the gathering of expert opinions. An IPA (Importance Performance Analysis) analysis is conducted to identify problems and suggest promoting policies for the Yeosu Port cruise industry. According to the IPA analysis results, in the major categories, the creation of cruise infrastructure, and the establishment of a support system were evaluated as weak items (high importance but low satisfaction), and sub-categories corresponding to the two major categories were identified as weak points. Based on the results of this analysis, measures to promote the cruise industry in Yeosu Port were presented as cruise infrastructure, route expansion, tourism product development, and support system. This study is expected to be utilized when establishing policies for constructing Yeosu Port as the hub port of maritime tourism in Korea.