• Title/Summary/Keyword: marine bio

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An Experimental Study on the Exhaust pollutant Reduction in Diesel Engine using a Rice-Bran Oil (미강유를 사용한 디젤기관에서의 배기오염물질 저감에 관한 실험적 연구)

  • 이준서
    • Journal of Advanced Marine Engineering and Technology
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    • v.22 no.6
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    • pp.754-762
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    • 1998
  • Exhaust emissions in diesel engine are affected by fuel properties but the reason for this is not clear. Especially the recent strong interest in using low-grade fuel demands extensibe investigation in order to clarify the exhaust emissions. Bio-Diesel oil has a great possibility to solve the pollution problem caused by the exhaust gas from diesel engine vehicles. The use of bio-oils in diesel engines has received considerable atten-tion to the forseeable depletion of world oil supplies. So bio-diesel oil has been attracted with attentions for alternative and clean energy source. The purpose of this paper is to evaluate the fea-sibility of the rice-bran oil for alternative fuel in a diesel engine with rgard to exhaust emis-sions.

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Effect of Antimicrobial Activity of the Glutaraldehyde Cross-linked Glucosamine (글루코사민-글루타르알데히드 가교결합체의 항균 효과)

  • Lee, Choon Geun;Hwang, You Jin;Park, Jae Kweon
    • Journal of Marine Bioscience and Biotechnology
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    • v.6 no.2
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    • pp.53-61
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    • 2014
  • This study was investigated the antimicrobial activity of glutaraldehyde cross-linked glucosamine. Glutaraldehyde was used as a cross-linker which specifically combines an amine-group of molecules. To optimize the mixing ratio of glutaraldehyde and glucosamine, mixing ratio was set up 1:1, 2:1, 3:1 and 0.5:1 in molarity, respectively. The optimum mixing ratio of glucosamine and glutaraldehyde was found to be 3:1 using thin layer chromatography based on the production of complex. Glucosamine-glutaraldehyde cross-linked complex (Ggcc) revealed significant antimicrobial activity toward PWG than F1, both microbial strains were isolated from porcine semen as antibiotics resistance bacteria (ARB). These results clearly demonstrate that Ggcc has potential bactericidal activity toward ARB in porcine semen.

Bio-functions of Marine Carotenoids

  • Hosokawa, Masashi;Okada, Tomoko;Mikami, Nana;Konishi, Izumi;Miyashita, Kazuo
    • Food Science and Biotechnology
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    • v.18 no.1
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    • pp.1-11
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    • 2009
  • Carotenoids being most important pigments among those occurring in nature, have received increased interest owing to their beneficial effects on human health. An effort is made to review marine carotenoids as important bioactive compounds with reference to their presence, chemical, and biofunctional benefits they afford. The potential beneficial effects of marine carotenoids were particularly focused on astaxanthin and fucoxanthin, major marine carotenoids found in marine animals and aquatic plants, respectively. Both carotenoids show strong antioxidant activity which is attributed to quenching singlet oxygen and scavenging free radicals. The potential role of the carotenoids as dietary antioxidants has been suggested as being one of the main mechanism by which they afford their beneficial health effects such as anticancer activity and anti-inflammatory effect. Only recently, antiobesity effect and antidiabetic effect have been noted as specific and novel bio-functions of fucoxanthin. Nutrigenomic study reveals that fucoxanthin induces uncoupling protein 1 (UCP1) expression in white adipose tissue (WAT) mitochondria to lead to oxidation of fatty acids and heat production in WAT. Fucoxanthin improves insulin resistance and decreases blood glucose level, at least in part, through the down-regulation of tumor necrosis factor $\alpha$ ($TNF{\alpha}$) in WAT of animals.

Immune Enhancement Effects of Codium fragile Anionic Macromolecules Combined with Red Ginseng Extract in Immune-Suppressed Mice

  • Kim, Ji Eun;Monmai, Chaiwat;Rod-in, Weerawan;Jang, A-yeong;You, Sang-Guan;Lee, Sang-min;Park, Woo Jung
    • Journal of Microbiology and Biotechnology
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    • v.29 no.9
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    • pp.1361-1368
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    • 2019
  • Codium fragile is an edible seaweed in Asian countries that has been used as a thrombolytic, anticoagulant, antioxidant, anti-inflammatory, and immune-stimulatory agent. Ginseng has also been known to maintain immune homeostasis and to regulate the immune system via enhancing resistance to diseases and microorganisms. In this study, anionic macromolecules extracted from C. fragile (CFAM) were orally administered with red ginseng extract (100 mg/kg body weight) to cyclophosphamide-induced immunosuppressed male BALB/c mice to investigate the immune-enhancing cooperative effect of Codium fragile and red ginseng. Our results showed that supplementing CFAM with red ginseng extract significantly increased spleen index, T- and B-cell proliferation, NK cell activity, and splenic lymphocyte immune-associated gene expression compared to those with red ginseng alone, even though a high concentration of CFAM with red ginseng decreased immune biomarkers. These results suggest that CFAM can be used as a co-stimulant to enhance health and immunity in immunosuppressed conditions.

Survival Rates with Time Course of Frozen-thawed Pacific Oyster Larvae in Indoor Rearing System

  • Kim, Ki Tae;Lim, Han Kyu;Chang, Young Jin
    • Development and Reproduction
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    • v.17 no.4
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    • pp.337-343
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    • 2013
  • Post-thawed larval rearing in Pacific oyster Crassostrea gigas was performed to investigate the survival rate with time course in three kinds of larvae cryopreserved. The highest survival rate and larval activity index (LAI) of post-thawed larvae were obtained from the permeation in 0.2 M sucrose and 2.0 M ethylene glycol (EG) at $-1^{\circ}C/min$ in freezing speed showing the survival rates just after thawing of 63.8% in trochophore, 84.1% in D-shaped veliger and 56.3% in early umbo veliger. In post-thawed larval rearing with food supply, the larvae lasted their lives until 24 hours in trochophore, 75 hours in D-shaped veliger and 57 hours in early umbo veliger. The results suggested that each larval stage post-thawed revealed no more further development to subsequent respective stage.

Graphiumins I and J, New Thiodiketopiperazines from the Marine-derived Fungus Graphium sp. OPMF00224

  • Fukuda, Takashi;Nagai, Kenichiro;Kurihara, Yuko;Kanamoto, Akihiko;Tomoda, Hiroshi
    • Natural Product Sciences
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    • v.21 no.4
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    • pp.255-260
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    • 2015
  • Two new thiodiketopiperazines (TDKPs), designated graphiumins I (1) and J (2), were isolated from the culture broth of the marine-derived fungus Graphium sp. OPMF00224 by solvent extraction, silica gel column chromatography, and HPLC. Their absolute structures were elucidated by spectroscopic analyses (1D and 2D NMR data, ROESY correlations, and CD data) and chemical methods. They were found to be structurally rare TDKPs with a phenylalanine-derived indolin substructure. Compounds 1 and 2 inhibited yellow pigment production by methicillin-resistant Staphylococcus aureus (MRSA) with $IC_{50}$ values of 63.5 and $76.5{\mu}g/ml$, respectively, without inhibiting its growth, even at $250{\mu}g/ml$.

The First Record of the Marphysa victori (Polychaeta, Eunicida, Eunicidae) from Korea, with DNA Barcode Data

  • Kim, Hana;Kim, Keun-Yong;Phoo, War War;Kim, Chang-Hoon
    • Animal Systematics, Evolution and Diversity
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    • v.37 no.1
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    • pp.1-8
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    • 2021
  • A eunicid polychaete, Marphysa victori Lavesque, Daffe, Bonifácio & Hutchings, 2017 is described for the first time from the intertidal zones of the Korean coasts. It is characterized by having three types of pectinate chaetae (INS, isodont-narrow-slender; AWS, anodont-wide-slender; and AWT, anodont-wide-thick), appearance of pectinate chaetae from chaetiger 2, the chaetae consisted of pectinate and compound spinigers, and pygidium with one pair of pygidial cirri. In genetic analysis based on cytochrome c oxidase subunit I (COI), intra-specific genetic distance between the specimens of M. victori from its type locality, France and Korea are in the range of 0.000-0.013. This paper includes the morphological description and photographs of M. victori new to Korean fauna, with partial sequences of the mitochondrial COI as DNA barcode data on this species.

The Effects of Starvation on Physiological Changes and Stress Response in Cultured Cobitid Loach (Misgurnus anguillicaudatus) Exposed to Sodium Nitrite

  • Hur, Jun Wook;Gil, Hyun Woo;Park, In-Seok
    • Journal of Marine Life Science
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    • v.3 no.2
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    • pp.87-95
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    • 2018
  • To investigate effects of starvation on physiological changes, stress response, and survival of cobitid loach (Misgurnus anguillicaudatus) exposed to sodium nitrite (NaNO2), a 4-week experiment was conducted. Fewer fish survived in the starved group than those in the fed group during the experiment. Starvation resulted in growth retardation, leading to differences in body length and body depth between fed and starved groups. The fed gorup continued to grow and remained in good condition. Blood chemical analysis (plasma cortisol and glucose) showed significant differences in stress response to nitrite exposure between fed and starved groups (p < 0.05). These results suggest that all parameters employed in this study to assess effects of starvation with NaNO2 stress are useful information for researching nutritional status in cobitid loach.

Preliminary EST analysis of immune-relevant genes from the liver of LPS-stimulated rock bream Oplegnathus fasciatus

  • Kim, Ju-Won;Park, Hyung-Jun;Baeck, Gun-Wook;Park, Chan-Il
    • Journal of fish pathology
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    • v.23 no.2
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    • pp.229-238
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    • 2010
  • We constructed a rock bream (Oplegnathus fasciatus) liver cDNA library and a total of 1533 expressed sequence tag (EST) clones were generated. Gene annotation procedures and homology searches of the sequenced ESTs were analyzed using BLASTX. Of the 1533 EST clones, 1165 different ESTs showed significant homology to previously described genes while 368 ESTs were unidentified, hypothetical, or unnamed proteins. Encoding 106 different sequences were identified as putative bio-defense genes or genes associated with immune response.

Effects of Feeding Rate and Water Temperature on Growth and Body Composition of Juvenile Korean Rockfish, Sebastes schlegeli (Hilgendorf 1880)

  • Mizanur, Rahman Md.;Yun, Hyeonho;Moniruzzaman, M.;Ferreira, F.;Kim, Kang-Woong;Bai, Sungchul C.
    • Asian-Australasian Journal of Animal Sciences
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    • v.27 no.5
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    • pp.690-699
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    • 2014
  • Three feeding trials were conducted to evaluate the effects of feeding rate and water temperature on growth and body composition of juvenile Korean rockfish, Sebastes schlegeli rearing at 3 different water temperatures. A total of 270 fish (each experiment) individual body weight (BW) averaging $16{\pm}0.3g$ ($mean{\pm}SD$) were fed a commercial diet for 4 wk at $16^{\circ}C$, $20^{\circ}C$, and $24^{\circ}C$. At each temperature, triplicate tanks were assigned to one of 6 feeding rates: 1.5%, 2.5%, 2.8%, 3.1%, 3.4%, and satiation (3.7% BW/d) at $16^{\circ}C$, 1.9%, 2.9%, 3.2%, 3.5%, 3.8% and satiation (4.1% BW/d) at $20^{\circ}C$ and 1.7%, 2.7%, 3.0%, 3.3%, 3.6%, and satiation (3.9% BW/d) at $24^{\circ}C$ water temperature. Weight gains of fish in satiation and 3.4% groups at $16^{\circ}C$, in satiation and 3.8% groups at $20^{\circ}C$ and in satiation and 3.6% groups at $24^{\circ}C$ were significantly higher than those of fish in the other treatments (p<0.05). A broken line regression analysis of weight gain indicated that optimum feeding rates of juvenile Korean rockfish were 3.41% at $16^{\circ}C$, 3.75% at $20^{\circ}C$ and 3.34% at $24^{\circ}C$ water temperature. Results of the present study indicate that the optimum feeding rate could be >3.1% but <3.41% at $16^{\circ}C$, >3.5% but <3.75% at $20^{\circ}C$ and >3.0% but <3.34% at $24^{\circ}C$. As we expected results suggest that fish performed better at $20^{\circ}C$ than $16^{\circ}C$ or $24^{\circ}C$ water temperature and the optimum feeding rate could be 3.1% BW/d to 3.7% BW/d in 16 g of juvenile Korean rockfish.