Preliminary EST analysis of immune-relevant genes from the liver of LPS-stimulated rock bream Oplegnathus fasciatus

  • Kim, Ju-Won (Department of Marine Biology & Aquaculture, Institute of Marine Industry, College of Marine Science, Gyeongsang National University) ;
  • Park, Hyung-Jun (Department of Marine Biology & Aquaculture, Institute of Marine Industry, College of Marine Science, Gyeongsang National University) ;
  • Baeck, Gun-Wook (Department of Marine Biology & Aquaculture, Institute of Marine Industry, College of Marine Science, Gyeongsang National University) ;
  • Park, Chan-Il (Department of Marine Biology & Aquaculture, Institute of Marine Industry, College of Marine Science, Gyeongsang National University)
  • Received : 2010.03.30
  • Accepted : 2010.08.20
  • Published : 2010.08.30

Abstract

We constructed a rock bream (Oplegnathus fasciatus) liver cDNA library and a total of 1533 expressed sequence tag (EST) clones were generated. Gene annotation procedures and homology searches of the sequenced ESTs were analyzed using BLASTX. Of the 1533 EST clones, 1165 different ESTs showed significant homology to previously described genes while 368 ESTs were unidentified, hypothetical, or unnamed proteins. Encoding 106 different sequences were identified as putative bio-defense genes or genes associated with immune response.

Keywords

References

  1. Azam, A., Paul, J., Sehgal, D., Prasad, J., Bhattacharya, S. and Bhattacharya, A.: Identification of novel genes from Entamoeba histolytica by expressed sequence tag analysis. Gene, 181:113-116, 1996. https://doi.org/10.1016/S0378-1119(96)00484-2
  2. Cho, Y.S., Choi, B.N., Kim, K.H., Kim, S.K., Kim D.S., Bang, I.C., Nam, Y.K.: Differential expression of Cu/Zn superoxide dismutase mRNA during exposures to heavy metals in rockbream (Oplegnathus fasciatus). Aquaculture, 253: 667-679, 2006. https://doi.org/10.1016/j.aquaculture.2005.05.047
  3. Clark, M.S., Edwards, Y.J., Peterson, D., Clifton, S.W., Thompson, A.J., Sasaki, M., Suzuki, Y., Kikuchi, K., Watabe, S., Kawakami, K., Sugano, S., Elgar, G. and Johnson, S.L.: Fugu ESTs: new resources for transcription analysis and genome annotation. Genome Res., 13:2747-2753, 2003. https://doi.org/10.1101/gr.1691503
  4. Feldmann G.: Tissue and cellular organisation of the liver, in: F. Tronche, M. Yaniv (Eds.), Liver Gene Expression, R.G. Landes Company, Austin, TX, pp. 17-34, 1994.
  5. Franco, G.R., Adams, M.D., Bento, S.M., Simpson, A.J.G., Venter, J.C. and Pena, S.D.J.: Identification of new Schistosoma mansonigenes by the EST strategy using a directional cDNA library. Gene, 152:141-147, 1995. https://doi.org/10.1016/0378-1119(94)00747-G
  6. Gish, W. and David, J.S.: Identification of protein coding regions by database similarity search. Nature Genetics, 3:266-272, 1993. https://doi.org/10.1038/ng0393-266
  7. Holland, M.C. and Lambris, J.D.: The complement system in teleosts. Fish Shellfish Immunol., 12:399-420, 2002. https://doi.org/10.1006/fsim.2001.0408
  8. Jung S.J. and Oh, M.J.: Iridovirus-like infection associated with high mortalities of striped beakperch, Oplegnathus fasciatus (Temminck et Schlegel) in southern coastal areas of the Korean peninsula. J. Fish Dis. 23:223-226, 2000. https://doi.org/10.1046/j.1365-2761.2000.00212.x
  9. Kondo, H., Morinaga, K., Misaki, R., Nakaya, M. and Watabe, S.: Characterization of the pufferfish Takifugu rubripes apolipoprotein multigene family. Gene, 346:257-266, 2005. https://doi.org/10.1016/j.gene.2004.11.015
  10. Lee, C.K., Weindruch, R. and Prolla, T.A.: Gene-expression profile of the aging skin in mice. Nat. Genet., 25:294-297, 2000. https://doi.org/10.1038/77046
  11. Manseth, E., Skjervold, P.O., Fjaera, S.O., Brosstad, F.R., Bjornson, S. and Flengsrud, R.: Purification and characterization of Atlantic salmon (Salmo salar) fibrinogen. Comp. Biochem. Physiol., Part B Biochem. Mol. Biol., 138:169-174, 2004. https://doi.org/10.1016/j.cbpc.2004.03.007
  12. Maruyama, K. and Sugano, S.: Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides. Gene, 138: 171-174, 1994. https://doi.org/10.1016/0378-1119(94)90802-8
  13. Nakabo, T.: Fishes of Japan with Pictorial Keys to the Species, English Edition II, Tokai University Press, Japan, pp. 956, 2002.
  14. Rise, M.L., von Schalburg, K.R., Brown, G.D., Mawer, M.A., Devlin, R.H., Kuipers, N., Busby, M., Beetz-Sargent, M., Alberto, R., Gibbs, A.R., Hunt, P., Shukin, R., Zeznik, J.A., Nelson, C., Jones, S.R., Smailus, D.E., Jones, S.J., Schein, J.E., Marra, M.A., Butterfield, Y.S., Stott, J.M., Ng, S.H., Davidson, W.S. and Koop, B.F.: Development and application of a salmonid EST database and cDNA microarray: data mining and interspecific hybridization characteristics. Genome Res., 14:478-490, 2004. https://doi.org/10.1101/gr.1687304
  15. Sohn, S.G., Choi, D.L., Do, J.W., Hwang, G.Y. and Park, J.W.: Mass mortalities of cultured striped beakperch, Oplegnathus fasciatus by iridoviral infection. J. Fish Pathol., 13:121-127, 2000.
  16. Ton, C., Hwang, D.M., Dempsey, A.A., Tang, H.C., Yoon, J., Lim, M., Mably, J.D., Fishman, M.C. and Liew, C.C.: Identification, characterization, and mapping of expressed sequence tags from an embryonic zebrafish heart cDNA library. Genome Res., 10:1915-1927, 2000. https://doi.org/10.1101/gr.10.12.1915
  17. Zelensky, A.N. and Gready, J.E.: C-type lectin-like domains in Fugu rubripes. BMC Genomics, 5:51, 2004. https://doi.org/10.1186/1471-2164-5-51
  18. Zeng, S. and Gong, Z.: Expressed sequence tag analysis of expression profiles of zebrafish testis and ovary. Gene, 10:45-53, 2002.