• Title/Summary/Keyword: mannitol

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Studies on Free-Sugars, Sugaralcohols, Amino acids and Mineral Contents in Edible Mushrooms. (버섯의 유리당, 당알콜, 아미노산 및 무기질의 조성에 관한 연구)

  • Hur, Yun-Haeng
    • Journal of environmental and Sanitary engineering
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    • v.4 no.2 s.7
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    • pp.27-32
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    • 1989
  • In orde to investigate free sugar, sugar alcohol, amino acid and mineral contents of edible mushrooms, hentinus edodes, Auricula-Jude and Coriolus Versicolor were analyzed. 1. In each of the three mushrooms, glucose, fructose, xylose, trehalose, mannose and mannitol were identified and in the hentinus edodes and Auricula-jude, the major free sugar was trehalose, respectively and increasing sequence in amount of free sugars were mannitol, mannose, fructose, glucose, on the other hand that lowest amount was Xylose. The relatively richest were glucose, fructose, trehalose, mannose, on the other hand lower amount of sample, Coriolus Versicolor were mannitol and Xylose. 2. In each of the three samples, essential amino acids were high amounts, especially good taste component, glutamic acid was higher. 3. The mineral p Contents$(925\~115mg)$ were highest and Mg was higher amount, in hentinus edodes and Auricula-jude, K, Na, Ca, Cu, Zn, etc were identified, In Coriolus versicolor, K content was highest, on the other hand, Sequence of mineral amount was Ca, Fe and Na.

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Optimization of Medium Components using Response Surface Methodology for Cost-effective Mannitol Production by Leuconostoc mesenteroides SRCM201425 (반응표면분석법을 이용한 Leuconostoc mesenteroides SRCM201425의 만니톨 생산배지 최적화)

  • Ha, Gwangsu;Shin, Su-Jin;Jeong, Seong-Yeop;Yang, HoYeon;Im, Sua;Heo, JuHee;Yang, Hee-Jong;Jeong, Do-Youn
    • Journal of Life Science
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    • v.29 no.8
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    • pp.861-870
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    • 2019
  • This study was undertaken to establish optimum medium compositions for cost-effective mannitol production by Leuconostoc mesenteroides SRCM201425 isolated from kimchi. L. mesenteroides SRCM21425 from kimchi was selected for efficient mannitol production based on fructose analysis and identified by its 16S rRNA gene sequence, as well as by carbohydrate fermentation pattern analysis. To enhance mannitol production by L. mesenteroides SRCM201425, the effects of carbon, nitrogen, and mineral sources on mannitol production were first determined using Plackett-Burman design (PBD). The effects of 11 variables on mannitol production were investigated of which three variables, fructose, sucrose, and peptone, were selected. In the second step, each concentration of fructose, sucrose, and peptone was optimized using a central composite design (CCD) and response surface analysis. The predicted concentrations of fructose, sucrose, and peptone were 38.68 g/l, 30 g/l, and 39.67 g/l, respectively. The mathematical response model was reliable, with a coefficient of determination of $R^2=0.9185$. Mannitol production increased 20-fold as compared with the MRS medium, corresponding to a mannitol yield 97.46% when compared to MRS supplemented with 100 g/l of fructose in flask system. Furthermore, the production in the optimized medium was cost-effective. The findings of this study can be expected to be useful in biological production for catalytic hydrogenation causing byproduct and additional production costs.

Production of Glucooligosaccharides and Mannitol from Leuconostoc mesenteroides B-742 Fermentation and its Separation from Byproducts

  • Chung Chang-Ho
    • Journal of Microbiology and Biotechnology
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    • v.16 no.2
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    • pp.325-329
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    • 2006
  • Leuconostoc mesenteroides B-742 fermentations with maltose as an acceptor were tested for glucooligosaccharides and mannitol co-production. Leuconostoc oligosaccharides were produced that were oligomers with a size range of DP 2 to 7 and were primarily DP 3, 4, 5, and 6, containing mainly ${\alpha}-1,4$ and ${\alpha}-1,6$ linkages. Maltose was linked to the reducing end of the isomaltosyl residues. The $Ca^{2+}$ form of cation-exchange column could separate glucooligosaccharides from byproducts.

Asthma Year in Review (호흡기내과 의사를 위한 천식 리뷰)

  • Kim, Sang-Ha
    • Tuberculosis and Respiratory Diseases
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    • v.69 no.6
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    • pp.411-417
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    • 2010
  • This review highlights articles pertaining to the following 5 topics: the relationship between asthma, allergic and non-allergic rhinitis; the novel asthma phenotypes using cluster analysis; the diagnostic properties of inhaled dry-powder mannitol for the diagnosis of asthma; the value of mepolizumab therapy in exacerbations of refractory eosinophilic asthma; the role of bronchial thermoplasty in the treatment of severe asthma.

Phytochemical study on randia siamensis

  • Lapikanon, Pattra;Tovivich, Phichai;Woo, Won-Sick;Choi, Jae-Sue
    • Archives of Pharmacal Research
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    • v.6 no.1
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    • pp.29-33
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    • 1983
  • From the roots of Pandia siamensis, D-mannitol, a mixture of .betha.-sitosterol and campesterol, oleanolic acid acetate, oleanolic acid-3-.alpha.-L-arabino side and mesembryanthemoidigenic acid as a sapogenin were isolated and characterized.

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Isolation and Characterization of Acetobacter Species from a Traditionally Prepared Vinegar (전통방식으로제조한식초로부터 Acetobacter 종들분리및특성조사)

  • Lee, Kang Wook;Shim, Jae Min;Kim, Gyeong Min;Shin, Jung-Hye;Kim, Jeong Hwan
    • Microbiology and Biotechnology Letters
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    • v.43 no.3
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    • pp.219-226
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    • 2015
  • Acetic acid bacteria (AAB) were isolated from vinegar fermented through traditional methods in Namhae county, Gyeongnam, the Republic of Korea. The isolated strains were Gram negative, non-motile, and short-rods. Three selected strains were identified as either Acetobacter pasteurianus or Acetobacter aceti by 16S rRNA gene sequencing. A. pasteurianus NH2 and A. pasteurianus NH6 utilized ethanol, glycerol, D-fructose, D-glucose, D-mannitol, D-sorbitol, L-glutamic acid and Na-acetate. A. aceti NH12 utilized ethanol, n-propanol, glycerol, D-mannitol and Na-acetate. These strains grew best at 30℃ and an initial pH of 3.4. They were tolerant against acetic acid at up to 3% of initial concentration (v/v). The optimum conditions for acetic acid production were 30℃ and pH 3.4, with an initial ethanol concentration of 5%, resulting in an acetic acid concentration of 7.3−7.7%.

The Effect of Culture Methods and Plant Growth Regulators on Bulblet Formation and Growth in Scale Segment Culture of Fritillaria thunbergii Miq. (패모 인편 배양시 자구 형성과 비대에 미치는 배양 방법과 생장 조절제의 처리 효과)

  • Paek, Kee-Yoeup;Yu, Kwang-Jin
    • Korean Journal of Medicinal Crop Science
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    • v.4 no.2
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    • pp.132-138
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    • 1996
  • This experiment was carried out to establish a year-round production system of pathogen-free stock through micropropagation in Fritillaria thunbergii as medicinal bulbous plant. The effect of different types of culture method and plant growth regulators, activated charcoal and mannitol on bulblet formation and subsequent growth were investigated. The MS solid medium containing 1. 0 mg/L kinetin and 0. 3 mg/L NAA was effective on bulblet formation and propagation rate compared to liquid and suspension culture. Addition of activated charcoal at 0. 01% to 0. 1% in the medium promoted bulbing of cultured bulblets and shoot formation. Addition of 1% to 2% mainnitol in MS medium was effective on the formation of bulblet and subsequent growth of bulblets compared to control. In addition of inhibitors, $10{\sim}100\;mg/L$ B-9 and Chloromequat had effective to stimulate bulblet growth but their effects were not so much as mannitol treatment. ABA treatment had detrimental effect on survival rate of explant and bulblet formation.

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The Effect of Various Humectants on Equilibrium Moisture Content and Storage Stability of Seasoned Squid (여러가지 보습제가 조미오징어 평형수분함량에 미치는 영향)

  • Rhee, Chul;Kang, Chang-Soo
    • Korean Journal of Food Science and Technology
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    • v.20 no.5
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    • pp.704-710
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    • 1988
  • This study was, firstly, to investigate water holding capacity in terms of variation of moisture sorption isotherms of seasoned squid treated with sodium lactate, glycerol, propylene glycol, sorbitol, mannitol, sodium benzoate, potassium sorbate and calcium propionate, and secondly, the effect of humectant treatments on storage stability was studied. The criteria for storage stability was based on three quality factors, namely, lipid oxiations, color development by non-enzymatic browning reactions and lipid oxidation, and mold growth. The effect of humectants on equilibrium moisture content was in the following increasing order; mannitol < sorbitol < sodium lactate < propylene glycol < glycerol. The experimental data indicated that sodium lactate has, in practice, potentially positive effect on processing of seasoned squid. During the storage period of 60 days, TBA values increased in all samples tested as humectants concentrations increased up to 10%. However, in the range of 1-7% sodium lactate treatment, the degree of lipid oxidation, browning reactions and mild growth were not high enough to affect the quality of seasoned squid, when compared with conventionally manufactured ones.

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Characterization of the Biosurfactant-Producing Bacterium, Pseudoalteromonas sp. HK-3 Isolated from the Crude-Oil Contaminated Areas (원유로 오염된 지역으로부터 분리한 생물계면활성제 생산균주, Pseudoalteromonas sp. HK-3의 특성조사)

  • Cho, Su-Hee;Oh, Kye-Heon
    • Korean Journal of Microbiology
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    • v.46 no.4
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    • pp.346-351
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    • 2010
  • The purpose of this work was to investigate the characteristics of a biosurfactant-producing bacterium isolated from crude-oil contaminated soils. During the incubation of strain HK-3 with 1% crude-oil, bacterial growth pattern, the amount of biosurfactant production, and pH changes were monitored. In order to examine the effect of supplemented carbons on the production of biosurfactant, cultivation of HK-3 cells in BH media with different carbons (e.g. glucose, dextrose, mannitol, citrate, or acetate) revealed that the production of biosurfactant reached the maximal level at the 72 h incubation with mannitol, which the area of clear zone was measured to approximately 7.64 $cm^2$. Identification test using the BIOLOG system, morphology study based on scanning electron microscopy and the 16S rRNA sequence-based phylogenetic analysis assigned strain HK-3 to a Pseudoalteromonas species, designated as Pseudoalteromonas sp. HK-3 which was registered in GenBank as [FJ477041].

Protoplast Isolation and Differentiation of Marine Green Alga Monostroma nitidum (해산 녹조류 참홑파래, Monostroma nitidum의 원형질체 분리와 분화)

  • CHO Yong Chul;GONG Yong Gun;YOON Jang-Taek;SUN Sang-Mi;CHUNG Gyu-Hwa
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.32 no.1
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    • pp.117-120
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    • 1999
  • High yields of protoplasts were obtained following enzymatic digestion of the vagetative thalli of marine green alga Monostroma nitidum. The enzyme mixtures containing $4\%$ Cellulase R-10+$3\%$ Macerozyme R-10+$3\%$ Abalone acetone power produced $4.41\times10^6$ protoplasts per 300 mg of fresh tissue. The highest yield of protoplasts was obtained by 270 minutes treatment of the thalli in enzyme solution. Freshly isolated protoplasts were spherical in shape and ranged between $13\~33\mu$m in diameter. The high efficiency of differentiation were obtained by incubating freshly isolated protoplasts in 0.4 M mannitol f/2 medium for 7 days and then transferring to 0.2 M mannitol f/2 medium. Protoplasts began to form new cell walls three days after initial culture and began to germinate after 10 days, and then form a leafy thallus after further culture in f/2 medium. The addition of antibiotics in media inhibited the differentiation of protoplasts in culture.

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