• 품질경영학회지
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• 제30권2호
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• pp.118-129
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• 2002

A multiple test of a mean parameter, λ, in the Poisson model is considered using the Bayes factor. Under noninformative improper priors, the intrinsic Bayes factor(IBF) of Berger and Pericchi(1996) and the fractional Bayes factor(FBF) of O'Hagan(1995) called as the default or automatic Bayes factors are used to select one among three models, M$_1$: λ< $λ_0, M$_2$: λ= $λ_0, M$_3$: λ> $λ_0. Posterior probability of each competitive model is computed using the default Bayes factors. Finally, theoretical results are applied to simulated data and real data.

• 한국수정란이식학회지
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• 제13권3호
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• pp.245-249
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• 1998

본 연구는 성장인자 EGF, IGF-1, EGF+IGF-1 이 소 난포란의 체외성숙에 미치는 영향을 규명하기 위하여 실시하였다. 소 난포란의 체외성숙 시 EGF를 농도별 (10, 50 및 100ng/m1)로 첨가하여 실험한 결과 통계학적 유의성은 인정되지 않았으나 24시간 배양 후 성숙율은 대조군에 비하여 높은 경향을 나타내었다. 소난포란의 체외성숙 시 EGF와 IGF-1을 병용 처리하여 실험한 결과, EGF 또는 IGF-1 단독처리 군에 비하여 병용처리군이 유의적으로 높은 체외성숙율을 나타내었다(p〈0.05). 따라서 소난포란의 체외성숙율은 EGF와 IGF-1에 의하여 영향을 받으며 특히 EGF와 IGF-1의 상호작용에 의하여 더 유효한 효과를 나타내는 것으로 사료된다.

• 대한본초학회지
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• 제28권5호
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• pp.113-119
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• 2013

Objectives : The purpose of this study was to investigate the effects of Angelicae Gigantis Radix Water Extract(AG) on the production of proinflammatory mediators in RAW 264.7 cells stimulated with lipopolysaccharide(LPS). Method : RAW 264.7 cells were cotreated with AG(50 and 100 ug/mL) and lipopolysaccharide(LPS; 1 ug/mL) for 24 hours. After 24 hour treatment, using Bead-based multiplex cytokine assay, concentrations of various cytokines such as interleukin(IL)-6, IL-$1{\beta}$, IL-10, tumor necrosis factor-alpha(TNF-${\alpha}$), granulocyte colony-stimulating factor(G-CSF), granulocyte macrophage colony-stimulating factor(GM-CSF), interferon inducible protein-10(IP-10), leukemia inhibitory factor(LIF), lipopolysaccharide-induced chemokine(LIX), monocyte chemoattractant protein-1(MCP-1), macrophage colony-stimulating factor(M-CSF), macrophage inflammatory protein(MIP)-$1{\alpha}$, MIP-$1{\beta}$, MIP-2, Regulated on Activation, Normal T cell Expressed and Secreted(RANTES) and vascular endothelial growth factor(VEGF) were measured. Result : AG significantly inhibited LPS-induced production of TNF-${\alpha}$, MIP-$1{\alpha}$, G-CSF, RANTES, IL-10, and M-CSF from LPS-stimulated RAW 264.7 cells at the concentrations of 50 and 100 ug/mL. AG significantly inhibited LPS-induced production of MIP-$1{\beta}$, MIP-2, GM-CSF, and IL-6 from LPS-stimulated RAW 264.7 cells at the concentrations of 50 ug/mL. AG significantly inhibited LPS-induced production of VEGF from LPS-stimulated RAW 264.7 cells at the concentrations of 100 ug/mL. But AG did not show any significant effect on the production of MCP-1, LIF, LIX, IP-10 and IL-$1{\beta}$ from LPS-induced RAW 264.7 cells. Conclusion : These results suggest that AG has anti-inflammatory effect related with its inhibition of proinflammatory mediators such as TNF-${\alpha}$, MIP-$1{\alpha}$, G-CSF, RANTES, IL-10, MIP-$1{\beta}$, MIP-2, GM-CSF, IL-6, VEGF and M-CSF in LPS-induced macrophages.

• BMB Reports
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• 제38권6호
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• pp.690-694
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• 2005

Transcription termination of the human mitochondrial genome requires specific binding to termination factor mTERF. In this study, mTERF was produced in E. coli and purified by two-step chromatography. mTERF-binding DNA sequences were isolated from a pool of randomized sequences by the repeated selection of bound sequences by gel-mobility shift assay and polymerase chain reaction. Sequencing and comparison of the 23 isolated clones revealed a 16-bp consensus sequence of 5'-GTG$\b{TGGC}$AGANCCNGG-3' in the light-strand (underlined residues were absolutely conserved), which nicely matched the genomic 13-bp terminator sequence 5'-$\b{TGGC}$AGAGCCCGG-3'. Moreover, mTERF binding assays of heteroduplex and single-stranded DNAs showed mTERF recognized the light strand in preference to the heavy strand. The preferential binding of mTERF with the light-strand may explain its distinct orientation-dependent termination activity.

• 정보저장시스템학회논문집
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• 제1권2호
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• pp.188-191
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• 2005

Two different coverlayers which is useful for an optical buffer and a mechanical protection made of not only UV resin but also polycarbonate coversheet were prepared on small form factor optical disks. Thin coverlayer of 10 ${\mu}m$ and thick coverlayer of 80 ${\mu}m$ were fabricated. 10 ${\mu}m$-thick coverlayer was coated using UV resin material by spin coating method for the flying optical head application. On the other hand, 80 ${\mu}m$-thick coverlayer using coversheet with the resin bonding material was prepared for the non-flying optical head application. Both cases, the thickness uniformity seem to be the primary prerequisite factor, and it was analyzed. Thickness of 10 ${\mu}m$-thick UV resin coverlayer could be controlled within ${\pm}0.2m$ range and 80 ${\mu}m$-thick coversheet could be controlled within ${\pm}3{\mu}m$ range. However, the yield of such thickness tolerance was not good. New design of metal housing holder and polycarbonate outer ring was adopted to diminish the ski-jump phenomenon. Specifically, the polycarbonate outer ring was very effective to reduce the ski-jump. However, it should be careful to maintain uniform edge between disk and ring for the perfect coverlayer.

• 한국전산구조공학회논문집
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• 제23권1호
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• pp.45-52
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• 2010

본 논문에서는 새로운 차량의 도입으로 건설되는 신형식 교량의 동적거동을 확인하기 위해 상용 유한요소 프로그램을 이용하여 해석을 실시하여 충격계수를 산정하였으며, 국내뿐만 아니라 각국에서 적용되고 있는 충격계수에 관한 설계기준을 조사하여 비교 검토하였다. 연구에서 제시되어진 교량형식은 파이프트러스 형식으로 각각 34m, 44m, 54m로 모델링하였으며 각각의 지간길이에 따른 충격계수의 영향을 산정하였다. 차량모델은 실제 교량 위를 통과하는 2축과 3축 저상굴절차량과 박영석(1997)이 제시한 147kN 덤프트럭 모델을 적용하였으며, 각 차량별로 10km/h~100km/h까지의 속도대역에 따른 충격계수를 산정하여 이에 따른 영향을 검토하였다. 또한 차량 및 지간길이에 따라 산정되어진 지간 중앙에서의 충격계수를 바탕으로 우리나라 및 외국의 설계규정과 비교 검토하였다.

• 한국산업보건학회지
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• 제28권3호
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• pp.283-291
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• 2018

Objectives: To monitor the radon concentration level in plants that handle phosphorus rock and produce gypsum board and cement, and evaluate the effective dose considering the effect of radon exposure on the human body. Methods: Airborne radon concentrations were measured using alpha-track radon detectors (${\alpha}$-track, Rn-tech Co., Korea) and continuous monitors (Radon Sentinel 1030, Sun Nuclear Co., USA). Radon concentrations in the air were converted to radon doses using the following equation to evaluate the human effects due to radon. H (mSv/yr) = Radon gas concentration x Equilibrium factor x Occupancy factor x Dose conversion factor. The International Commission on Radiological Protection (ICRP) used $8nSv/(Bq{\cdot}hr/m^3)$ as the dose conversion factor in 2010, but raised it by a factor of four to $33nSv/(Bq{\cdot}hr/m^3)$ in 2017. Results: Radon concentrations and effective doses in fertilizer manufacturing process averaged $14.3(2.7)Bq/m^3$ ($2.0-551.3Bq/m^3$), 0.11-0.54 m㏜/yr depending on the advisory authority and recommendation year, respectively. Radon concentrations in the gypsum-board manufacturing process averaged $14.9Bq/m^3$ at material storage, $11.4Bq/m^3$ at burnability, $8.1Bq/m^3$ at mixing, $10.0Bq/m^3$ at forming, $8.9Bq/m^3$ at drying, $14.7Bq/m^3$ at cutting, and $10.5Bq/m^3$ at shipment. It was low because it did not use phosphate gypsum. Radon concentrations and effective doses in the cement manufacturing process were $23.2Bq/m^3$ in the stowage area, $20.2Bq/m^3$ in the hopper, $16.8Bq/m^3$ in the feeder and $11.9Bq/m^3$ in the cement mill, marking 0.12-0.63 m㏜/yr, respectively. Conclusions: Workers handling phosphorous gypsum directly or indirectly can be assessed as exposed to an annual average radon dose of 0.16 to 2.04 mSv or 0.010 to 0.102 WLM (Working Level Month).

• 한국지반공학회:학술대회논문집
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• 한국지반공학회 2010년도 춘계 학술발표회
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• pp.1302-1308
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• 2010

This study the change of the safety factor before and after the reinforcement were compared by performing the parameter research based on the limit equilibrium analysis regarding the same cross section after carrying out the safety factor before the reinforcement on the virtual section in order to observe the change of the safety factor of the slop reinforced with the slope planting reinforced earth, and the variation of the safety factor according to the increase of the length of the reinforcement materials and the change of the slope height was analyzed. As the result, the reinforcement effect was insignificant at no more than 0.6 of L/H, the reinforcement length ratio when the reinforcement length was increased, as the increase of the safety factor was slow comparing with the non-reinforced slope. At 3.0m of the slope height, reinforcement on the slope is not necessary, and at 3.0m to 5.0m of the slope height, the inclination was not influencing at no less than 0.6 of L/H. At 5.0m to 9.0m of the slope height, the safety factor was mostly secured on the slope at 0.8 of L/H and the over-reinforced slope appeared at no less than 1.0 of L/H. Also, the safety factor increased as the slope height increases and the slope gets steeper till 0.8 of L/H, but the slope steepness affects more on the increase of the safety factor than the reinforcement material, as the reinforcing force by the reinforcement material became steady.

• 한국산업보건학회지
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• 제29권3호
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• pp.343-350
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• 2019

Objectives: This study aims to identify whether ventilation conditions and their effectiveness can be significantly improved in an experimental chamber by increasing the mixing factor (K-Factor). Methods: In a chamber with a volume of $1m^3$, air velocity was measured at six different points with four roof fans in the upper part of the chamber being operated in order. The impact of the ventilation conditions was analyzed when the flow rates were increasing and the first inlet of the chamber was either open or closed. Smoke patterns were also observed at four corner points where ventilation was limited. Kruskal Wallis and Mann-Whitney tests were performed to compare air velocities measured in the chamber. Results: The air velocities measured at only the third point increased significantly from $0.03{\pm}0.03m/s$ (door open) and $0.05{\pm}0.06m/s$ (door closed) with two fans, $0.08{\pm}0.08m/s$ with three fans, and $0.09{\pm}0.09m/s$ with four fans operating (p<0.05). However, air velocities at the four corner points did not significantly increase. Smoke patters also showed that the open inlet of the chamber had no effect on improvement of ventilation conditions and effectiveness. Conclusions: In this study, the air velocities at six points in the chamber did not significantly increase despite the increase in the mixing factor and flow rates of ventilation in the controlled environment. Therefore, the inflow of outdoor air throughout an open inlet and installation of a forced ventilation system can potentially increase the indoor air velocity and improve ventilation condition without an increase in the mixing factor.

• Reproductive and Developmental Biology
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• 제36권1호
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• pp.13-19
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• 2012

In order to provide the basis for developing practical mouse embryonic stem cells (mESCs) culture method, how the endogenous level of self-renewal-stimulating factor genes was altered in the mESCs by different extracellular signaling was investigated in this study. For different extracellular signaling, mESCs were cultured in 2 dimension (D), 3D and integrin-stimulating 3D culture system in the presence or absence of leukemia inhibitory factor (LIF) and transcriptional level of $Lif$, $Bmp4$ and $Wnt3a$ was evaluated in the mESCs cultured in each system. The expression of three genes was significantly increased in 3D system relative to 2D system under LIF-containing condition, while only $Wnt3a$ expression was increased by 3D culture under LIF-free condition. Stimulation of integrin signaling in mESCs within 3D system with exogenous LIF significantly up-regulated transcriptional level of $Bmp4$, but did not induce transcriptional regulation of $Lif$ and $Wnt3a$. In the absence of LIF inside 3D system, the expression of $Lif$ and $Bmp4$ was significantly increased by integrin signaling, while it significantly decreased $Wnt3a$ expression. Finally, the signal from exogenous LIF significantly caused increased expression of $Lif$ in 2D system, decreased expression of $Bmp4$ in both 2D and 3D system, and decreased expression of $Wnt3a$ in integrin-stimulating 3D system. From these results, we identified that endogenous expression level of self-renewal-stimulating factor genes in mESCs could be effectively regulated through artificial and proper manipulation of extracellular signaling. Moreover, synthetic 3D niche stimulating endogenous secretion of self-renewal-stimulating factors will be able to help develop growth factor-free maintenance system of mESCs.