• Journal of Korean Clinical Nursing Research
• /
• v.28 no.2
• /
• pp.146-156
• /
• 2022

Purpose: The purpose of this study was to examine the influence of empowerment, infection control organizational culture, and infection control awareness on infection control performance among nurses in long-term care hospitals. Methods: A descriptive survey study was conducted with 125 nurses as subjects who had been working for at least six months in four long-term care hospitals located in Busan metropolitan city and Gyeongsangnam-do Province. Data were collected from September 30 to October 28, 2021 and analyzed using t-test, one-way ANOVA, Pearson's correlation coefficients, and stepwise multiple regression with SPSS/WIN 26.0. Results: The results showed that infection control performance had significant correlations with empowerment (r=.36, p<.001), infection control organizational culture (r=.51, p<.001), and infection control awareness (r=.75, p<.001). Multiple regression analysis for infection control performance revealed that the most powerful predictor was infection control awareness (β=.70, p<.001). Empowerment, infection control awareness and awareness of infection control guidelines explained approximately 60.0% of the variance in infection control performance. Conclusion: Findings indicated that various factors are related to the infection control performance among nurses in long-term care hospital. Based on the results of this study, further development and application of the programs to enhance empowerment and infection control awareness are needed in order to improve the infection control performance of nurses in long-term care hospitals.

• Journal of Animal Reproduction and Biotechnology
• /
• v.38 no.3
• /
• pp.177-187
• /
• 2023

Background: The grading of Hanwoo (Korean native cattle) is based on four economic traits, and efforts have been continuously made to improve the genetic traits associated with these traits. There is a technology to predict the expected grade based on the 4 economic genetic SNP characteristics of Korean cattle calves using single nucleotide polymorphism (SNP) technology. Selection of highly proliferative, self-renewing, and differentiating satellite cells from cattle is a key technology in the cultured meat industry. Methods: We selected the Hanwoo with high and low-scored of genomic estimated breeding value (GEBV) by using the Hanwoo 50K SNP bead chip. We then isolated the bovine satellite cells from the chuck mass. We then conducted comparative analyses of cell proliferation, immunocytochemistry, qRT-PCR at short- and long-term culture. We also analyzed the differentiation capability at short term culture. Results: Our result showed that the proliferation was significantly high at High scored GEBV (Hs-GEBV) compared to Low scored GEBV (Ls-GEBV) at short- and long-term culture. The expression levels of Pax3 were significantly higher in Hs-GEBV bovine satellite cells at long-term culture. However, there were no significant differences in the expression levels of Pax7 between Hs- and Ls-GEBV bovine satellite cells at short- and long- term culture. The expression levels of MyoG and MyHC were significantly high at Ls-GEBV bovine satellite cells. Conclusions: Our results indicated that selection of bovine satellite cells by Hanwoo 50K SNP bead chip could be effective selection methods for massive producing of satellite cells.

• Korean Journal of Plant Resources
• /
• v.36 no.6
• /
• pp.588-596
• /
• 2023

Long-term culture of cell lines is an important issue in in vitro culture and in plant science. In this study, the regeneration ability and ex vitro acclimatization of regenerants were evaluated. The ploidy level of regenerants derived from long-term cultured cell lines was measured in Imperata cylindrica 'Rubra', Poaceae. Adventitious buds (shoots) were successfully induced from five-year-cultured calli on MS medium containing 0.1 mg/L BA or 2.0 mg/L TDZ, combined with 0.01 mg/L auxins (IAA, IBA, NAA and 2,4-D), respectively. Adventitious roots were also induced on MS medium containing 0.01 mg/L auxins (IBA, NAA and 2,4-D), respectively. Interestingly, regenerants with both red and green leaf were successfully obtained when regenerants were cultured on MS medium with 9% sucrose. Regenerants derived from long-term cultured calli were transferred to pots using an optimal acclimatization process and successfully adapted to both pot and soil conditions. Moreover, the ploidy level was measured using calli and regenerants that had been kept on MS medium containing various kinds of plant growth regulators (PGRs).

• The Journal of the Korea Contents Association
• /
• v.15 no.11
• /
• pp.192-201
• /
• 2015

The purposes of this study were to understand the organizational culture and to explore factors related to organizational commitment. The nationwide mailing survey was conducted using structured questionnaires from February 7th, 2013 to February 18th, 2013. With regard to the organizational culture, a relationship-oriented culture was predominant. The relationship-oriented culture(${\beta}=0.45,p<0.001$) and innovation-oriented culture(${\beta}=0.34,p<0.001$) had a positive influence on organizational commitment. This study has 56% of the explanatory power(F=14.83,p<0.001). The long-term care facilities with higher evaluation grades showed higher organizational commitment(F=7.18,p<0.001), which affected positively the evaluation of long-term care facilities (${\beta}=0.53,p<0.001$). On the basis of the above results, the conclusions were as follows: First, managers in the long-term care facilities should strive to build good relationships with staffs as to achieve greater organizational commitment and higher evaluation scores. Second, more researches are needed to find out how to improve the relationships among staffs.

• Journal of Korean Public Health Nursing
• /
• v.33 no.1
• /
• pp.98-108
• /
• 2019

Purpose: The purpose of this study was to identify factors influencing care workers' patient safety behaviors (PSB), we hoped to provide baseline data for its activation. Methods: The subjects of this study were chosen by convenience sampling, which included 114 care workers at long term care facilities. Data were collected from August 22 to October 10, 2018. The gathered data were analyzed using the SPSS program, which was used to Cronbach's alpha, descriptive statistics, t-test, ANOVA, Pearson correlation coefficient and stepwise regression. Results: The care workers' average PSB was at 7.81 points, patient safety culture (PSC) 3.86 points and safety of long-term care facilities 7.07. PSB positively correlated with safety of facilities(r=.802, p<.001) and PSC(r=.558, p<.001). Work attitude and satisfaction of job were explained 35.1% of the PSB. Conclusion: Administrators of long term care facilities should be interested in patient safety culture and improving job satisfaction of care workers in order to improve patient safety behaviors.

• Journal of Digital Convergence
• /
• v.17 no.9
• /
• pp.231-240
• /
• 2019

The purpose of this study was to explore and describe the relationship of the perception of patient safety culture, knowledge of standard precaution and performance of standard precaution among long-term care hospital nurses. Participants were 134 nurse in long-term hospital. Data were collected with structured questionnaires from January 2 to June 4, 2018. Data were analyzed by SPSS/WIN 21.0 program for descriptive statistics t-test, ANOVA, Scheffe's test and Pearson's correlation coefficient. The perception of patient safety culture were significantly correlated with knowledge of standard precaution(r=.192, p=.027), performance of standard precaution(r=211, p=.014). Therefore, in order to enhance the perception of patient safety culture and performance of standard precaution of long-term care hospital nurses, it would be necessary to enable health-care associated infections by themselves by cultivating standard precaution knowledge through systemic education and by enabling the establishment of affirmative patient safety culture.

• Journal of Microbiology and Biotechnology
• /
• v.10 no.3
• /
• pp.321-326
• /
• 2000

To examine the feasibility of the long-term production of the human granulocyte colony stimulating factor (hG-CSF) using the $_{L}-arabinose$ promoter system of Escherichia coli, flask relay culture and cyclic fed-batch culture were performed. In the flask relay culture, it was found that the pismid was maintained stably up to about 170 generations in an uninduced condition, whereby the cells could also maintain the capability of expressing hG-CSF expression were maintained stably up to at least 100 generations. In contrast, in the cyclid fed-batch culture, segregational plasmid instability was observed within about 4 generations after induction, even though the cell growth and hG-CSF production reached their maximum balues, 78.0 g/l of dry cell weight and 7.0 g/l of hG-CSF, respectively. It would appear that, when compared to the flask relay culture, the high-cell density and high-level expression of hG-CSF in the cyclic fed-batch cultrure led to the segregational plasmid instability; in other words, a severe metabolic burden existe on the cells due to the high-level expression of hG-CSF. Accordingly, based on these long-term cultures, the segregational and structural plasmid instability was observed and a strategy to overcome such problems could be designed.

• Journal of Embryo Transfer
• /
• v.27 no.3
• /
• pp.189-192
• /
• 2012

Although embryonic stem cells (ESCs) or ES-like cells are reported from many mammalian species other than the mouse, the culture system for murine ESCs may not be suitable to the other species. Previously many other research groups have modified either human or mouse ESC culture systems for bovine ESC culture. In this study, we compared three different culture mediums consisting of DMEM, ${\alpha}$-MEM or KnockOut$^{TM}$-DMEM (KO), which are modified from human or mouse ESC culture system, for the generation of bovine ESCs. In this study, some pre-requisite events which are important for establishment and long-term propagation of ESCs such as inner cell mass (ICM) attachment on feeder cells, primary colony formation and sustainability after passaging. Once the ICM clumps attached on feeder cells, this was designated as passage 0. In regards to the rate of ICM attachment, ${\alpha}$-MEM was superior to the other systems. For primary colony formation, there was no difference between DMEM and ${\alpha}$-MEM whereas KO showed lower formation rate than the other groups. For passaging, the colonies were split into 2~4 pieces and passed every 5~6 days. From passage 1 to passage 3, DMEM system seemed to be appropriate for maintaining putative bovine ESCs. On the other hand, ${\alpha}$-MEM tended to be more suitable after passage 6. Although ${\alpha}$-MEM support to maintain a ES-like cell progenies to passage 15, all three culture systems which are modified from human or mouse ESC culture media failed to retain the propagation and long-term culture of putative bovine ESCs. Our findings imply that more optimized alternative culture system is required for establishing bovine ESC lines.

• International Journal of Advanced Culture Technology
• /
• v.5 no.4
• /
• pp.57-62
• /
• 2017

In this paper, we applied long short term memory(LSTM) for classifying political polarity in cyber public sphere. The data collected from the cyber public sphere is transformed into word corpus data through word embedding. Based on this word corpus data, we train recurrent neural network (RNN) which is connected by LSTM's. Softmax function is applied at the output of the RNN. We conducted our proposed system to obtain experimental results, and we will enhance our proposed system by refining LSTM in our system.

• International Commerce and Information Review
• /
• v.11 no.1
• /
• pp.67-92
• /
• 2009

This study investigates the relationship between Culture and online trust in three cultural dimensions: individualism, collectivism and long term orientation. For this purpose, this study proposes the empirical hypothesis to test the implication of the proposed model. The results are as follows. First, the purchase intention is influenced by the online trust in statistical sense. Second, the relationship between Culture and the antecedents of online trust are statistically established in three cultural dimensions: individualism, collectivism and long term orientation. The direction of future research is to develop the more refined hypothesis to test the empirical model in the field of on line trust and culture.