• 사료
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• s.31
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• pp.28-34
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• 2008

• Korean Journal of Environmental Agriculture
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• v.33 no.1
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• pp.63-67
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• 2014

BACKGROUND: The present study is an exploratory research to establish national biosafety regulation systems through a survey on the recognition of safety regulation systems for the living modified organism(LMO). METHODS AND RESULTS: We have conducted a survey on an awareness of LMO safety regulation systems in scientific working groups. The data of 235 respondents were analyzed using various statistical methods. As a result, 72.8% of the respondents were male; 27.2% were female, and 43.4% of them work in the university. A total of 33.2% of the respondents majored in general biology, and their most common job position was the laboratory safety manager. The difference of an awareness on LMO law and regulatory system was not statistically significant by either work places or job positions. CONCLUSION: For the rapid settlement and the efficient implementation of LMO safety management policy, we conclude that it is required to reduce the gap between the recognition and fulfillment of safety management. Furthermore, the mutual exchanges of information among researchers are needed with the settlement of the safety management system and the harmony of policy with improvement of the absurd regulations. The ongoing and specialized training, inspections, and the strengthening of public relations are also required along with the efforts to improve the absurd regulations.

• The Plant Pathology Journal
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• v.20 no.3
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• pp.206-211
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• 2004

Transgenic Nicotiana benthamiana plants harboring coat protein (CP) gene of Zucchini green mottle mosaic virus (ZGMMV) were generated for virus-resistant screening and complementation analysis of related viruses for environmental safety assessment (SA) of living modified organism (LMO) purposes. Transformation of leaf disc of N.benthamiana was performed by using Agrobacterium-mediated method and the pZGC-PPGA748 containing the ZGMMV CP and NPTII genes. Two kinds of transgenic homozygous groups, virus-resistant and virus-susceptible N.benthamiana lines, were obtained by screening of challenging homologous virus for Tl generations. These two pathologically different lines can be useful for host-virus interactions and LMO environmental SA.

• Korean Journal of Environmental Biology
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• v.36 no.4
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• pp.488-497
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• 2018

Quantitative classification of insect fauna in transgenic rice in 2 LMO(Living genetically Modified Organism) isolated paddy fields (Gyeongsang National University, Gyeongbuk National University) and rice in 4 paddy fields(Duryang 1, Duryang 2, Hwagye 1, Hwagye 2), were evaluated for consecutive 5 years (2013 to 2017) in Sacheon, Gyeongnam province, and for 2 consecutive years (2015 to 2016) in Gunwi Gyeongbuk province. Sampling insect fauna were evaluated by black light trapping, sticky trapping, visual surveying, and sweeping methods in each target paddy field, respectively. A total of 37,941 individuals, of 464 species from 15 orders, were collected in Sacheon for 5 years. A total of 10,030 individuals, of 366 species from 13 orders, were collected in Gunwi for 2 years. Based on results of comparison between transgenic and non-transgenic paddy fields for 5 consecutive years, the similarity index between LMO paddy field and common paddy field is not different. Thus, this difference is not due to the environment, not the LMO.

• Journal of Plant Biotechnology
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• v.42 no.3
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• pp.196-203
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• 2015

AbstractLiving modified organisms (LMO) are one of the most widespread products of modern biotechnology after DNA discovery. Due to the decline of grain self-sufficiency rate and the increase of reliance on LMO imports in Korea, a series of concerns with regard to safety of living modified(LM) crops has been raised. The aim of this study is to establish the detection methods for unintentional release or growing of LMO plants in environmental conditions. To detect LM crop events, general concepts of specific primer design and PCR conditions were provided by the Joint Research Centre (JRC). The certified reference materials of seven LM events (4 soybean, 2 cotton and 1 corn) were obtained from the Institute for Reference Materials and Measurements (IRMM) and the American Oil Chemists' Society (AOCS). Genomic DNA from seven LM events were purified and PCR amplifications were carried out by using individual event-specific primer sets. LM-specific PCR products of all seven events were efficiently amplified by our methods. The results indicate that the established detection method for LMOs is suitable as a scientific tool to monitor whether the crops found in natural environments are LMOs.

• Food Science and Industry
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• v.52 no.2
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• pp.140-152
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• 2019

"Contained use" means using LMOs during manufacturing processes within a facility, equipment or other physical structures to prevent the spread of LMOs to the external environment. LMOs and their substances not only have played an essential role in various industries in different ways, but also have made to spread concerns in Biosafety widely. In Korea, the LMO Act is to implement the Cartagena Protocol on Biosafety' since 1st Jan 2008. This is focused on Biosafety and Risk management for LMOs according to the precautionary principle.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.146.1-146
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• 2003

Transgenic Nicotiana benthmiana plants harboring and expressing coat protein (CP) gene of Zucchini green mottle mosaic virus (ZGMMV) were generated for both virus-resistant screening and complementation analysis of related viruses and environmental safety assessment (SA) of living modified organism (LMO) purposes. Transformation of leaf disc of N. benthamiana was performed using Agrobacterium-mediated method and the pZGCPPGA748 containing the ZGMMV CP and NPTII genes. Two kinds of transgenic homozygous groups, virus-resistant and -susceptible lines, were obtained by screening of challenging homologous virus for T1 generations. Complementation of CP-deficient related virus was analyzed using the susceptible line of ZGMMV. These two pathologically different lines can be useful for host-virus interactions and LMO environmental SA.

• Journal of Life Science
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• v.32 no.8
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• pp.641-646
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• 2022

There are concerns about the environmental release of living modified organism (LMO) maize created to increase yields. In fact, there are cases in which LMO crops for feed have been leaked in Korea to form autoite colonies, and concerns about LMO spill are intensifying. In this study, the possibility of environmental outflow and occurrence of native organisms was analyzed using maize feed and seeds distributed in Korea. In the evaluation of the possibility of spontaneous occurrence of maize in the event of an unintentional release of maize feed made by crushing maize, the incidence rate of maize was 0.01%, which was extremely low compared to the germination rate of maize seeds. A survey of the dormant rate of maize showed that all maize seeds collected every month were dead. In the germination rate test by temperature using Daehak wax corn and Kwangpyeongok, high germination rates were found at 20℃ and 30℃, and relatively low germination rates were found at 10℃ and 40℃. In addition, all germination tests showed a higher germination rate Daehak wax corn than Kwangpyeongok. The difference between domestic and overseas cultivation maize was confirmed through a survey on the agricultural properties of three varieties of maize. The data obtained through this experiment could be the basis for the evaluation of the weediness potential of environmental risk assessment and technology to suppress the occurrence of autoite in preparation for future LMO spills.

• Journal of Applied Biological Chemistry
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• v.61 no.4
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• pp.305-314
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• 2018

'New Breeding Techniques (NBTs)' have been one of hot issues, since their future will be affected profoundly by national as well as international regulatory landscapes. In this review, we compare characteristics of NBTs with conventional and genetic modification, and analyze genetically modified organism (GMO) regulatory systems in the context of possible regulation of NBTs. NBTs are very heterogeneous in terms of principles, methodologies, and final products. As Living Modified Organisms (LMO) is defined in the Cartagena Protocol on Biosafety (CPB) as an organism containing novel combination of genetic materials obtained by the use of modern biotechnology, CPB as well as other national legislations locate itself somewhere in the middle between product-based and process-based regulations. It is also noted that jurisdictions with regulatory systems more oriented to product-based one tend to be more productive and decide or may decide to exempt site-directed nucleases-1 from GMO regulation. In this context, Korean legislations are reviewed to clarify the commons and differences in GMO definitions. Act on Transboundary Movement of LMO Act, Food Sanitation Act and Agricultural and Fishery Products Quality Control Act are three major acts to regulate GMOs. It is noted that there are differences in the definition of LMO or GM food/products especially between the LMO Act and the Food Sanitation Act. Such differences may cause conflicts between Acts when policy-decision regarding the regulation of NBTs is made. Therefore, it is necessary to reorganize legislations before policies regarding the regulation of any techniques from biotechnology are made.

• The Plant Pathology Journal
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• v.22 no.4
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• pp.353-359
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• 2006

Transgenic Nicotiana benthamiana plants harboring the coat protein(CP) gene of Zucchini green mottle mosaic virus(ZGMMV) were chosen as a model host for the environmental risk assessment of genetically modified plants with virus resistance. This study was focused on whether new virus type may arise during serial inoculation of one point CP mutant of ZGMMV on the transgenic plants. In vitro transcripts derived from the non-functional CP mutant were inoculated onto the virus-tolerant and -susceptible transgenic N. benthamiana plants. Any notable viral symptoms that could arise on the inoculated transgenic host plants were not detected, even though the inoculation experiment was repeated a total of ten times. This result suggests that potential risk associated with the CP-expressiing transgenic plants may not be significant. However, cautions must be taken as it does not guarantee environmental safety of these CP-mediated virus-resistant plants, considering the limited number of the transgenic plants tested in this study. Further study at a larger scale is needed to evaluate the environmental risk that might be associated with the CP-mediated virus resistant plant.