Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Establishment of detection methods for approved LMO in Korea

국내 승인 유전자변형 작물의 검출 기법 확립

  • Seol, Min-A (Department of Eco-safety, Bureau of Conservation Ecology, National Institute of Ecology (NIE)) ;
  • Lee, Jung Ro (Department of Eco-safety, Bureau of Conservation Ecology, National Institute of Ecology (NIE)) ;
  • Choi, Wonkyun (Department of Eco-safety, Bureau of Conservation Ecology, National Institute of Ecology (NIE)) ;
  • Jo, Beom-Ho (Department of Eco-safety, Bureau of Conservation Ecology, National Institute of Ecology (NIE)) ;
  • Moon, Jeong Chan (Department of Eco-safety, Bureau of Conservation Ecology, National Institute of Ecology (NIE)) ;
  • Shin, Su Young (Department of Eco-safety, Bureau of Conservation Ecology, National Institute of Ecology (NIE)) ;
  • Eum, Soon-Jae (Department of Eco-safety, Bureau of Conservation Ecology, National Institute of Ecology (NIE)) ;
  • Kim, Il Ryong (Department of Eco-safety, Bureau of Conservation Ecology, National Institute of Ecology (NIE)) ;
  • Song, Hae-Ryong (Department of Eco-safety, Bureau of Conservation Ecology, National Institute of Ecology (NIE))
  • 설민아 (국립생태원 생태보전연구본부 위해생물연구부) ;
  • 이중로 (국립생태원 생태보전연구본부 위해생물연구부) ;
  • 최원균 (국립생태원 생태보전연구본부 위해생물연구부) ;
  • 조범호 (국립생태원 생태보전연구본부 위해생물연구부) ;
  • 문정찬 (국립생태원 생태보전연구본부 위해생물연구부) ;
  • 신수영 (국립생태원 생태보전연구본부 위해생물연구부) ;
  • 엄순재 (국립생태원 생태보전연구본부 위해생물연구부) ;
  • 김일룡 (국립생태원 생태보전연구본부 위해생물연구부) ;
  • 송해룡 (국립생태원 생태보전연구본부 위해생물연구부)
  • Received : 2015.05.15
  • Accepted : 2015.06.17
  • Published : 2015.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

AbstractLiving modified organisms (LMO) are one of the most widespread products of modern biotechnology after DNA discovery. Due to the decline of grain self-sufficiency rate and the increase of reliance on LMO imports in Korea, a series of concerns with regard to safety of living modified(LM) crops has been raised. The aim of this study is to establish the detection methods for unintentional release or growing of LMO plants in environmental conditions. To detect LM crop events, general concepts of specific primer design and PCR conditions were provided by the Joint Research Centre (JRC). The certified reference materials of seven LM events (4 soybean, 2 cotton and 1 corn) were obtained from the Institute for Reference Materials and Measurements (IRMM) and the American Oil Chemists' Society (AOCS). Genomic DNA from seven LM events were purified and PCR amplifications were carried out by using individual event-specific primer sets. LM-specific PCR products of all seven events were efficiently amplified by our methods. The results indicate that the established detection method for LMOs is suitable as a scientific tool to monitor whether the crops found in natural environments are LMOs.

2008년 이후 꾸준히 유전자변형생물체의 수입 승인이 증가하여 2015년 2월 기준 국내 131개의 이벤트가 식용, 사료용, 가공용으로 수입 및 유통 중이다. LMO의 비의도적 자연 생태계 유출을 파악하기 위해 선행되어야 하는 것은 명확한 LMO 도입유전자 검출 기법의 개발이며, 본 연구를 통해 2014년 7개 이벤트에 대한 도입유전자 검출 기법을 확립하였다. 현재까지 확보된 유전자 분석기법은 40개이며 이는 국내도입 LMO의 80%를 검출할 수 있는 과학적 근거를 제공할 것이다(Lee et al. 2015). LMO 자연환경 모니터링 및 사후관리 연구를 위해 단일이벤트를 검출할 수 있는 기법 연구는 앞으로 계속 수행할 계획이다.

Keywords

References

  1. Agbios (2001) Esential Biosafety. Vol. 1 No. 1 Merkvile, Canada
  2. BIOSAFETY SCANNER. Available from: http://en.biosafetyscanner.org/
  3. Center for Environmental Risk Assessment (CERA). Available from: http://www.cera-gmc.org/
  4. Charles Delobel C. Bonfini L, Querci M, Mazzara M. Cordell S, Van Den Eede G (2011) Event specific method for the quantification of Soybean MON87701 using real time PCR. Validation report and protocol.EUR25136EN.JRC 68071
  5. Eom SH, Kim DI, Choi C (2013) Study on Environmental monitoring and post-management of LMO(VI). NIE-2014-0039.
  6. European Commission, Joint Research Centre (JRC). Available from: http://gmo-crl.jrc.ec.europa.eu
  7. GMO Detection Method Database (GMDD). Available from: http://gmdd.shgmo.org/index/search
  8. International Service for the Acquisition of Agri-biotech Applications (ISAAA). GM Approval Database. Available from: http://www.isaaa.org. Accessed 2014
  9. Kim TS, Song HR, Kim SJ, Hur MS, Kim JM (2011) Establishment of LMO detection methods. NIER-RP2011-1347.
  10. Korea Biosafety Clearing House(KBCH). Status of risk assessment of GMO in Korea. Available from: http://www.biosafety.or.kr. Accessed 2015
  11. Lee B, Kim CG, Park JY, Yi H, Park KW, Jeong WK, An JH, Cho KH, Kim HM (2007) Survay of herbicide resistant oilseed rapes around the basin of rivers in incheon harbor area. Kor. J. Weed Sci. 27:29-35
  12. Lee JR, Choi WK, Moon JC, Jo BH, Shin SY, Seol MA, Eum SJ, Kim IR, Song HR, Kim JM (NIE) (2015) LMO detection methods. ISBN 979-11-86197-24-0
  13. Mazzara M, Cordell S, Van Den Eede G (2006) Event specific method for the quantification of hybrid cotton line 281-24-236/3006-210-23 using real time PCR. Validation report and protocol. EUR22473EN
  14. Mazzara M, Cordell S, Van Den Eede G (2012) Event specific method for the quantification of cotton GHB119 using real time PCR. Validation report and protocol.EUR22528EN.JRC75655
  15. Ministry of agriculture food and rural affairs. Self-sufficiency rate of grain in Korea. Available from: http://www.mafra.go.kr. Accessed 2015
  16. Park KW, Kim CK, Lee R, Kim DY, Park JY, Kim DI, Kwon MC, Yi H, Kim HM (2007) Monitoring of imported genetically modified crops in the cultivated fields in Korea. Kor. J. Weed Sci.27:318-324
  17. Savini C, Bonfini L, Querci M, Mazzara M. Cordell S, Van Den Eede G (2012a) Event specific method for the quantification of Soybean MON87705 using real time PCR. Validation report and protocol. EUR25499EN.JRC 74376
  18. Savini C, Bonfini L, Querci M, Mazzara M. Cordell S, Van Den Eede G (2012b) Event specific method for the quantification of Maize MON87460 using real time PCR. Validation report and protocol. EUR25486EN.JRC 74374
  19. Savini C, Nardini E, Mazzara M. Cordell S, Kreysa J (2012c) Event specific method for the quantification of Soybean FG72 using real time PCR. Validation report and protocol. EUR254-03EN.JRC 73091
  20. Savini C, Mazzara M. Cordell S, Van Den Eede G (2011) Event specific method for the quantification of Soybean CV127 using real time PCR. Validation report and protocol. EUR-25132EN.JRC 68070
  21. Seralini GE, Clair E, Mesnage R, Gress S, Defarge N, Malatesta M, Hennezuin D, Joel Spiroux de Vendomois (2012) Long term toxicity of a Roundup herbicide and a Roundup-tolerant genetically modified maize. Food and Chemical Toxicology 50:4221-4231 https://doi.org/10.1016/j.fct.2012.08.005
  22. Yoon JH, Lee JE, Song HR, Kim SJ, Kim YH, Choi JE, Choi HL (2012) Establishment of LMO detection methods(II). NIERRP2012-280

Cited by

  1. Development of detection methods for six approved LM crops in Korea vol.44, pp.1, 2017, https://doi.org/10.5010/JPB.2017.44.1.097