Browse > Article
http://dx.doi.org/10.5423/PPJ.2006.22.4.353

Study on Environmental Risk Assessment for Potential Effect of Genetically Modified Nicotiana benthamiana Expressing ZGMMV Coat Protein Gene  

Kim, Tae-Sung (LMO Environmental Risk Evaluation Team, Ecosystem Disturbance Assessment Division, Nature and Ecology Research Department, National Institute of Environmental Research)
Yu, Min-Su (LMO Environmental Risk Evaluation Team, Ecosystem Disturbance Assessment Division, Nature and Ecology Research Department, National Institute of Environmental Research)
Koh, Kong-Suk (LMO Environmental Risk Evaluation Team, Ecosystem Disturbance Assessment Division, Nature and Ecology Research Department, National Institute of Environmental Research)
Oh, Kyoung-Hee (LMO Environmental Risk Evaluation Team, Ecosystem Disturbance Assessment Division, Nature and Ecology Research Department, National Institute of Environmental Research)
Ahn, Hong-Il (Plant Virus GenBank, Division of Environmental and Life Sciences, Seoul Women's University)
Ryu, Ki-Hyun (Plant Virus GenBank, Division of Environmental and Life Sciences, Seoul Women's University)
Publication Information
The Plant Pathology Journal / v.22, no.4, 2006 , pp. 353-359 More about this Journal
Abstract
Transgenic Nicotiana benthamiana plants harboring the coat protein(CP) gene of Zucchini green mottle mosaic virus(ZGMMV) were chosen as a model host for the environmental risk assessment of genetically modified plants with virus resistance. This study was focused on whether new virus type may arise during serial inoculation of one point CP mutant of ZGMMV on the transgenic plants. In vitro transcripts derived from the non-functional CP mutant were inoculated onto the virus-tolerant and -susceptible transgenic N. benthamiana plants. Any notable viral symptoms that could arise on the inoculated transgenic host plants were not detected, even though the inoculation experiment was repeated a total of ten times. This result suggests that potential risk associated with the CP-expressiing transgenic plants may not be significant. However, cautions must be taken as it does not guarantee environmental safety of these CP-mediated virus-resistant plants, considering the limited number of the transgenic plants tested in this study. Further study at a larger scale is needed to evaluate the environmental risk that might be associated with the CP-mediated virus resistant plant.
Keywords
Coat protein; environmental risk assessment; genetically modified; living modified organism; Nicotiana benthamiana; Tobamovirus; transgenic plant; Zucchini green mottle mosaic virus;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Falk, B. W., Passmore, B. K., Watson, M. T. and Chin, L-S. 1995. The specificity and significance of heterologous encapsidation of virus and virus-like RNA's. In: Biotechnology and Plant Protection. Viral pathogenesis and disease resistance, ed. by S-D. Kang and D. D. Bill, pp. 391-451. World Scientific, Singapore
2 Matthews, R. E. F. 1991. Plant Virology, Acadamic Press, New York, 835pp
3 Nagy, P. D. and Simon, A. E. 1997. New insight into the mechanisms of RNA recombination. Virology 235:1-9   DOI   ScienceOn
4 OECD, 1996. Consensus document on general information concerning the biosafety of crop plants made virus resistant through coat protein gene-mediated protection, OECD/ GD(96) 162:20-29
5 Powell Abel, P., Nelson, R. S., De, B., Hoffman, N., Rogers, S. G., Fraley, R. T. and Beachy, R. N. 1986. Delay of disease development in transgenic plants that express the tobacco mosaic virus coat protein gene. Science 232:738-743   DOI
6 Sacristan, S. Malpica, J. M., Fraile, A. and Garcia-Arenal, F. 2003. Estimation of population bottlenecks during systemic movement of tobacco mosaic virus in tobacco plants, J. Virol. 77:9906-9911   DOI
7 Wintermantel, W. M. and Schoelz, J. E. 1996. Isolation of recombinant viruses between cauliflower mosaic virus and a viral gene in transgenic plants under conditions of moderate selection pressure. Virology 223:156-164   DOI   ScienceOn
8 Kim, M. J., Choi, S. H., Kim, T., Park, M. H., Lim, H. R., Oh, K. H., Kim, T., Lee, M. H. and Ryu, K. H. 2004. Virus resistant and susceptible transgenic Nicotiana benthamiana plants expressing coat protein gene of Zucchini green mottle mosaic virus for LMO safety assessment. Plant Pathol. J. 20:206-211   DOI   ScienceOn
9 Fuchs, M., Gal-On, A., Raccah, B. and Gonsalves, D. 1999. Epidemiology of an aphid nontransmissible potyvirus in fields of nontransgenic and coat protein transgenic squash. Transgenic Res. 8:429-439   DOI   ScienceOn
10 Bau, H. J., Cheng, Y. H., Yu, T. A., Yang, J. S., Liou, P. C., Hsiao, C. H., Lin, C. Y. and Yeh, S. D. 2004. Field evaluation of transgenic papaya lines carrying the coat protein gene of Papaya ringspot virus in Taiwan. Plant Dis. 88:594-599   DOI   ScienceOn
11 Aaziz, R. and Tepfer, M. 1999a. Recombination between genomic RNAs of two cucumoviruses under conditions of minimal selection pressure. Virology 263:282-289   DOI   ScienceOn
12 Ito, W., Ishiguro, H. and Kurosawa, Y. 1991. A general method for introducing a series of mutations into cloned DNA using the polymerase chain reaction. Gene 102:37-37
13 Li, H. G. and Roossinck, M. J. 2004. Genetic bottlenecks reduced population variation in an experimental RNA virus population. J. Virol. 78:10582-10587   DOI   ScienceOn
14 Ryu, K. H., Min, B. E., Choi, G. S., Choi, S. H., Kwon, S. B., Noh, G. M., Yoon, J. Y., Choi, Y. M., Jang, S. H., Lee, G. P., Cho, K. H. and Park, W. M. 2000. Zucchini green mottle mosaic virus is a new tobamovirus: comparison of its coat protein gene with that of kyuri green mottle mosaic virus. Arch. Virol. 145:2325-2333   DOI   ScienceOn
15 Sambrook, J., Fritsch, E. F. and Maniatis, T. 2001, Molecular cloning: a laboratory manual, 2nd ed., Cold Springer Harbor Laboratory Press, New York
16 Borja, M., Rubio, T., Scholthof, H. B. and Jackson, A. O. 1999. Restoration of wild-type virus by double recombination of tombusvirus mutants with a host transgene. Mol. PlantMicrobe Interact. 12:153-162   DOI   ScienceOn
17 de Zoeten, G. A. 1991. Risk assessment: Do we let history repeat itself? Phytopathology 81:585
18 Aaziz, R. and Tepfer, M. 1999b. Recombination in RNA viruses and in virus-resistant transgenic plants. J. Gen. Virol. 80:1339-1346   DOI
19 Hammond, J., Lecoq, H. and Raccah, B. 1999. Epidemiological risks from mixed virus infections and transgenic plants expressing viral genes. Adv. Virus Res. 54:189-314   DOI
20 Thomas, P. E., Hassan, S., Kaniewski, W. K., Lawson, E. C. and Zalewski, J. C. 1998. A search for evidence of virus/transgene interactions in potatoes transformed with the potato leafroll virus replicase and coat protein genes. Mol. Breed. 4:407-417   DOI   ScienceOn
21 Lai, M. M. C. 1992. RNA recombination in animal and plant viruses. Microbiol. Rev. 56:61-79
22 Fuchs, M. Klas, F. E., McFerson, J. R. and Gonsalves. D. 1998. Transgenic melon and squash expressing coat protein genes of aphid-borne viruses do not assist the spread of an aphid nontransmissible strain of cucumber mosaic virus in the field. Transgenic Res. 7:449-462   DOI