• Weed & Turfgrass Science
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• 제3권4호
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• pp.342-346
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• 2014

본 연구에서는 골프장 잔디재배 토양에 축적되어 과습을 유발하여 잔디뿌리의 호흡작용을 방해하고 각종 병의 서식처가 되는 토양대취의 분해여부를 조사하기 위해 2012년부터 2년간 토양온도가 높은 기간인 6월부터 10월까지 인천시 중구 소재 스카이72 골프크럽 하늘코스 2홀 페어웨이에서 배양미생물과 가축분뇨액비를 혼용 처리하여 토양화학성, 잔디품질 및 토양 중의 대취분해여부에 대해 조사하였다. 공시 배양미생물은 연구소자체 선발하여 예비실험에서 대취분해 효과가 있었던 6종의 혼합미생물을 1톤 규모로 대량 배양하여 시험에 사용하였으며, 배양미생물에는 가축분뇨액비와 배양미생물의 토양 중 먹이역할을 하는 탄소화합물함유 아미노산액을 혼용하여 처리하였다. 시험결과, 처리구별 토양의 화학성과 미량원소 함유량은 큰 차이는 보이지 않았으나 엽색지수와 엽록소지수 및 잔디뿌리길이는 거의 동일하게 나타났다. 또한 토양 중 대취함량은 무처리보다 배양미생물과 가축분뇨액비를 혼용한 처리구에서 대취분해정도가 더 높게 나타났다. 따라서 한지형잔디로 식재된 골프코스 페어웨이 관리 시에 잔디품질 유지와 토양 중 대취분해를 위해서는 배양미생물과 가축분뇨액비의 혼합한 시비가 관행적인 화학비료시비보다 더 유리하다고 사료된다.

• 한국지역사회생활과학회지
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• 제17권4호
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• pp.39-48
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• 2006

This study was carried out to make a search for the usefulness of marigold plants (Tagetes erecta) as a natural dye. And we have also examined into the dyeability and antifungal activity of silk and cotton fabrics dyed with 2% powdered colorant and 1% liquid colorant. Almost surface colors of silk and cotton fabrics dyed with 2% powdered colorant and that dyed with 1% liquid colorant except for that treated Cu and Fe with mordants were shown by Y-level. $L^*$ values of silk fabrics dyed with 2% powdered colorant were 66.2 to 86.39, those $a^*$ values were -1.97 to 6.09, and those $b^*$ values were 13.69 to 35.97. And $L^*$ values of cotton fabrics dyed with 2% powdered colorant were 78.97 to 89.62, those a values were -3.39 to 0.38, and those $b^*$ values were 5.63 to 15.61 $L^*$ values of silk fabrics dyed with 1% liquid colorant were 34.37 to 85.57, those a values were -7.79 to 12.33, and those $b^*$ values were 15.13 to 82.91, And $L^*$ values of cotton fabrics dyed with 1% liquid colorant were 44.12 to 87.90, those $a^*$ values were -8.37 to 6.18, and those $b^*$ values were 11.65 to 78.87. Colorfastness of silk fabrics against light treated nothing with mordants and that dyed with 1% liquid colorant decreased for a little by second grade, however, that against washing, rubbing, perspiration and dry cleaning were increased over fourth grade. Colorfastness of cotton fabrics in terms of rubbing, light, and dry cleaning, except for washing and perspiration, were shown by over third grade, when that was treated nothing with mordants. Antifungal activities of silk fabrics treated nothing with mordants and dyed with 1% liquid colorant of marigold extracts were shown by 28.9% against Staphylococcus aureus. However, those of Al, Ca, Cu and tartaric acid mordanting were more than 25.5% against Staphylococcus aureus and Klebsiella pneumoniae.

• 미생물학회지
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• 제42권2호
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• pp.83-88
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• 2006

국내에서 식용버섯으로 이용되고 있는 8종 균주의 자실체와 균사체로부터 콜레스테롤 합성을 저해하는 lovastatin 생산과 고지혈증 억제활성을 탐색하였다. TLC 분석으로 lovastatin생산 유무를 검색한 결과 자실체와 균사체의 추출물에 대해 Rf값이 표준물질콰 같은 약 0.46에서 뚜렷한 밴드가 형성됨을 확인하였다. 자실체로부터 lovastatin 추출을 위한 용매로는 water/acetonitrile/methanol (5:2.5:2.5)을 사용하였을 매 가장 효과적인 lovastatin 생산량을 보였다. Lovastatin 생산량은 자실체 중에서는 Pleurotus ostreatus가 0.98 mg/g (dry biomass)으로 가장 높은 생산량을 나타냈고 균사체 배양액 추출물에 대해서도 P. ostreatus가 21.90 mg/L로 높은 생산량을 나타냈다. 3-Hydroxy-3-methylglutaryl-CoA (HMG-CoA) reductase 저해활성에서는 자실체에서 P ostreatus가 67.8%, 균사체에서 P. ostreatus와Laetiporus sulphureus가 각각 37.2%, 29.1%의 저해활성을 보였다. L. sulphureus는 lovastatin 생산량에 비해 높은 저해활성을 나타내서 lovastatin 이외의 다른 고지혈증 억제물질을 GC/MS로 분석한 결과 대사산물로 분비되는 식물성 sterol 물질인 campesterol과 gamma-sitosterol이 검출되었다.

• Journal of Ginseng Research
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• 제24권3호
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• pp.123-127
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• 2000

인삼모상근의 생장에 영향을 미치는 auxin과 CH의 효과를 규명하기 위하여 auxin류인 IBA, MA, NAA와 아미노산 화합물인 CH를 각각 농도별로 처리하여 MS 고체배지와 액체배지에서 50일간 배양후 수거하여 생중량과 건중량을 측정하여 농도별로 생장률을 측정한 결과, 인삼모상근의 생장에 미치는 auxin의 효과는 고체배양시에는 IBA를 1 mg/L의 농도로 암배양하는 것이 가장 효과적이었으며, NAA 1 mg/L의 농도로 광배양 하는 것도 효과적이었다. 반면에 IAA와 CH는 인삼모상근의 생장에 효과가 없었다. 액체배양시에는 IAA와 NAA를 처리했을 때 농도의 증가에 따른 생장의 증가는 보이지 않고 생장의 감소 없이 거의 일정하게 그 수준이 유지되었으며 IBA와 CH는 모두 인삼모상근의 생장을 오히려 억제하는 경향이었다.

• 대한의생명과학회지
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• 제22권4호
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• pp.215-219
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• 2016

The problems of tuberculosis and its drug resistance are very severe. Therefore, rapid and accurate drug susceptibility assay is required. Recently, there has been an increased understanding of the genetic mechanism of Mycobacterium tuberculosis (MTB) drug resistance as well as advancement of molecular technologies. While many gene mutations correlate well with drug resistance, many genes do not show a strong correlation with drug resistance. For this reason, the current study assessed the utility of rpoB mRNA as a target to detect live mycobacteria. In this study, RT-PCR targeting of rpoB mRNA in BCG treated with rifampin was performed. Conventional RT-PCR and real-time PCR targeting rpoB mRNA as well as 85B mRNA was performed to determine whether these two methods could distinguish between viable and non-viable MTB. The levels of rpoB and 85B mRNA detected by RT- PCR were compared in parallel with colony forming unit counts of BCG that were treated with rifampin for different periods of time. The data suggests that that even though both mRNA levels of rpoB and 85B decreased gradually when rifampin-treatment increased, the rpoB mRNA seemed to represent live bacteria better than 85B mRNA. This study clearly indicates that RT-PCR is a good method to monitor viable cell counts in the liquid culture treated with the anti-tuberculosis drug.

• 한국식생활문화학회지
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• 제35권3호
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• pp.278-284
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• 2020

In this study, biotin (vitamin B7) contents of frequently consumed foods in Korea were determined by using immunoaffinity column in conjunction with high-performance liquid chromatography (HPLC). The biotin contents of 24 foods of plant origin and 27 foods of animal origin were selected. The highest biotin contents in frequently consumed foods of plant origin were found in red beans (Huinguseul; 11.475 ㎍/100 g). On the other hand, biotin was not detected in any varieties of sorghum. For frequently consumed foods of animal origin, salted pollack roe (7.486 ㎍/100 g) showed the highest biotin content. However, beef and fish contained less biotin. All biotin analyses were conducted under analytical quality control. The limits of detection and limits of quantification of biotin were 0.007 and 0.023 ㎍/100 g, respectively, and the accuracy/recovery percentage was 95.35-105.02%. The precision values were 4.041% (repeatability) and 3.835% (reproducibility). Taken together, our data provide reliable data on the biotin contents of frequently consumed foods in Korea.

• Mycobiology
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• 제33권2호
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• pp.84-89
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• 2005

Five types of agricultural wastes were used for the production of xylanolytic enzyme by Aspergillus flavus K-03. All wastes materials supported high levels of xylanase and ${\beta}-xylosidase$ production. A high level of proteolytic activity was observed in barley and rice bran cultures, while only a weak proteolytic activity was detected in corn cob, barley and rice straw cultures. Maximum production of xylanase was achieved in basal liquid medium containing rice barn as carbon source for 5 days of culture at pH 6.5 and $25^{\circ}C$. The xylanolytic enzyme of A. flavus K-03 showed low thermostability. The times required for 50% reduction of the initial enzyme activity were 90 min at $40^{\circ}C$, 13 min at $50^{\circ}C$, and 3 min at $60^{\circ}C$. Xylanolytic activity showed the highest level at pH $5.5{\sim}10.5$ and more than 70% of the original activity was retained at pH 6.5 and 7.0. The higher stability of xylanolytic enzymes in the broad range of alkaline pH is useful for utilization of the enzymes in industrial process requiring in alkaline conditions. Moreover, the highest production of xylanolytic enzyme was obtained when 0.5% of rice bran was supplied in basal liquid medium. SDS-PAGE analysis revealed a single xylanase band of approximately 28.5 kDa from the culture filtrates.

• 원예과학기술지
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• 제32권5호
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• pp.694-701
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• 2014

Allium hookeri L. (Alliaceae family) is an important ethnomedicinal plant native to the Himalayan region of Asia. The aim of this research was to establish a high-frequency plant regeneration system for in vitro propagation of A. hookeri. Among the tissue types examined, receptacle explants derived from immature flower buds showed the highest regeneration rate of shoots ($93.33{\pm}4.63%$), roots ($76.67{\pm}7.85%$), and calli ($80.00{\pm}7.43%$) when cultured on Gamborg B5 (B5) medium containing $10{\mu}M$ 6-benzylaminopurine (BA) + $1{\mu}M$ naphthalene acetic acid (NAA), $0.5{\mu}M$ BA + $5{\mu}M$ NAA, and $1{\mu}M$ BA + $10{\mu}M$ NAA, respectively. Shoot multiplication was superior when cultured in liquid rather than on solid medium and relatively high concentrations of BA, ranging from 5 to $10{\mu}M$. Efficient bulblet formation following root induction from shoot clumps was achieved with culture in liquid B5 medium containing 7% (w/v) sucrose. Regenerated bulblets were successfully acclimatized to ex vitro conditions with a greater than 95% survival rate. By this method, a maximum of 62 plantlets per receptacle could be propagated within 9 weeks of initial culture. The in vitro propagation system established in this study will promote A. hookeri biotechnology, including large-scale production of healthy and aseptic clones, preserving parental genotypes with desirable traits, and genetic manipulation to enhance medicinal value.

• Plant Resources
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• 제6권2호
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• pp.153-158
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• 2003

Effects of shaking, medium consistency and anther density on polyhaploid production in two wheat cultivars, Pavon and Chris, were studied using a modified 85D12 medium. Pavon produced more calli in shaking and more albino plants tban Chris. However, Chris produced threefold more green plants than Pavon in non-shaking treatment. More calli and green plants were derived from non-shaking treatment than those from shaking treatment. Anthers were cultured on both liquid and semi-solid 85D12 media, using two anther densities, 48 and 96 anthers per plate. Although Pavon generally produced more calli and albino plants than Chris, Chris produced more green plants than Pavon. More green plants were derived from semi-solid medium than those from liquid medium. A factor that may affect plant regeneration from anthers is the length of time on initiation medium. Most of the calli for both genotypes were transferred during the first two time periods. Fertility, as measured by seed set, was determined for all surviving regenerated plants. About 24% of Chris and Pavon anther-derived green plants in the experiment of medium consistency and anther density produced seed.

• Journal of Plant Biotechnology
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• 제33권2호
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• pp.117-122
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• 2006

Comparative studies on medium supply in bioreactors (raft, immersion and ebb and flood) have revealed that multiplication and growth of Alocasia Amazonica were greatest in the raft system, while lowest in ebb and flood system. In the raft system, the basal part of the shoots was continuously in contact with medium, which enabled a constant uptake of nutrients as well as aeration to the explants. The number and the size of leaf stomata were higher in the raft system compared with immersion and ebb&flood system. In the immersion system, plantlets were deformed and epidermal cells in leaves were irregular with a large intercellular space. The results suggested that the medium supply should be controlled properly to maintain normal and healthy plantlets during liquid cultures in bioreactors Which affects morphology and physiology Of the plantlets.