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Screening of Edible Mushrooms for the Production of Lovastatin and its HMG-CoA Reductase Inhibitory Activity  

Lee Jae-Won (Department of Forest Sciences, College of Agriculture & Life Sciences, Seoul National University)
Lee Soo-Min (Department of Forest Sciences, College of Agriculture & Life Sciences, Seoul National University)
Gwak Ki-Seob (Department of Forest Sciences, College of Agriculture & Life Sciences, Seoul National University)
Lee Ji-Yoon (NICEM, College of Agriculture & Life Sciences, Seoul National University)
Choi In-Gyu (Department of Forest Sciences, College of Agriculture & Life Sciences, Seoul National University)
Publication Information
Korean Journal of Microbiology / v.42, no.2, 2006 , pp. 83-88 More about this Journal
Abstract
This research was performed to determine the production of lovastatin and its HMG-CoA reductase activity produced by fruit bodies and mycelial liquid cultures of domestic edible mushrooms (8 fungal strains). By deter-mining TLC analysis for the confirmation of the presence of lovastatin, all the extracts from fruit bodies and mycelial liquid culture showed same Rf value (0.46), whick was identical to that of the standard lovastatin. In order to extract lovastatin from fruit body, the mixture of water/acetonitrile/methanol was chosen as the most effective solvent. Extracts from fruit body and mycelial liquid culture of pleurotus ostreatus produced the high-est lovastatin 0.98 mg/g based on dry biomass, and 21.90 mg/L, respectively. In the inhibition rate of 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) reductase, the highest was obtained in P. ostreatus as 67.8% among fruit bodies, and the rates of mycelial liquid culture extracts from P. ostreatus and Laetiporus sulphureus were 37.2% and 29.1%, respectively. Unusually L. sulphureus showed high inhibition rate with low content of lovastatin due to the contribution of campesterol and gamma-sitosterol with hypocholesterolemic activity as metabolites.
Keywords
campesterol; gamma-sitosterol; 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) reductase; Laetiporus sulphureus; lovastatin; Pleurotus ostreatus;
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