• KSBB Journal
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• v.30 no.3
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• pp.119-124
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• 2015

Arthrospira platensis (A. platensis) is a microscopic and filamentous cyanobacterium that derives its name from the spiral or helical nature of its filaments. In this study, we induced mutants of A. platensis through NMU treatment and selected two strains by level of ipid contents. We named mutant '1-9', '2-5', and they were cultivated in the same way with the wild type. During 12 days cultivation, cell growth, dry cell weight, pigment content, and lipid content were measured for characteristics of mutants. As a result, pigment and lipid content of mutants were increased about 3.6, 1.8 times compared with wild type, respectively. It was shown that total flavonoid and polyphenol contents of mutants were increased about 1.5 times compared with wild type. And radical scavenging effect of mutants were increased about 10% compared with wild type.

• The Korean Journal of Food And Nutrition
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• v.7 no.4
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• pp.318-322
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• 1994

Aluminium alloy, comprising water, silicone manganese and porous aluminium carrier added into soybean oil in order to investigated its effect on polar lipid content, polymer content, conjugated dienoic acid and free fatty acid value during deep-fat frying at 185$^{\circ}C$. Increase rates of polar lipid and polymer content of the frying ell were reduced about one thirds of the oil without aluminium alloy during deep-fat frying. The aluminium alloy, however, have no significantly effect to inhibit the increase of conjugated dienoic acid and free fatty acid value. Treatment of the frying oil with aluminium alloy was found to be able to inhibit polymer and polar lipid formation.

• Proceedings of the Korean Society of Developmental Biology Conference
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• 2005.07a
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• pp.51-51
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• 2005

We inestigated the reproductive cycle with ovarian development of Chlamys farreri by histological observations, and seasonal changes in biochemical components of the adductor muscle, digestive diverticula and ovary were studied by biochemical analysis. The reproductive cycle of this species can be classified into five successive stages: early active stage (January to March), late active stage (March to April), ripe stage (April to August), partially spawned stage (June to August) and spent/inactive stage (August to January). According to ANOVA test, there were significant differences (p<0.05) in total protein, total lipid and glycogen contents among months for all of the adductor muscle, digestive diverticula and ovary. Total protein contents in ovary and digestive diverticula showed significant changes(ANOVA, p<0.05) during the study period, while that in the adductor muscle did not. Total protein content was highest in the adductor muscle, followed by ovary, and lowest in digestive diverticula. There was no correlation in total protein content between the adductor muscle and digestive diverticula (p=0.220). But strong positive correlation was found between adductor muscle and ovary (r=0.450, p=0.013). ANOVA showed that there were significant differences in total lipid and glycogen contents among months for all of the adductor muscle, ovary, and digestive diverticula (p<0.05). The monthly changes in total lipid content were highly variable in ovary and digestive gland. High contents of total lipid were foung during April and May-June in ovary, while March and June-July in digestive diverticula. There was a strong negative correlation in total lipid content between ovary and digestive diverticula (r=- 0.397, p=0.030). Unlike total protein of total lipid, glycogen content in the adductor muscle was most dynamic. It showed more than 36-fold changes in the adductor muscle (at most 3-fold change in ovary) during the study period. Glycogen content was higher during May-July in the adductor muscle, While it was higher in March and august in digestive diverticula. There was a strong negative correlation in glycogen content between the adductor muscle and digestive diverticula (r=-0.584, p=0.001).

• The Korean Journal of Malacology
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• v.21 no.1
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• pp.71-80
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• 2005

We investigated the reproductive cycle with ovarian development of Chlamys farreri by histological observations, and seasonal changes in biochemical components of the adductor muscle, digestive diverticula and ovary were studied by biochemical analysis. The reproductive cycle of this species can be classified into five successive stages: early active stage (January to March), late active stage (March to April), ripe stage (April to August), partially spawned stage (June to August) and spent/inactive stage (August to January). According to ANOVA test, there were significant differences (p < 0.05) in total protein, total lipid and glycogen contents among months for all of the adductor muscle, digestive diverticula and ovary. Total protein contents in ovary and digestive diverticula showed significant changes (ANOVA, p < 0.05) during the study period, while that in the adductor muscle did not. Total protein content was highest in the adductor muscle, followed by ovary, and lowest in digestive diverticula. There was no correlation in total protein content between the adductor muscle and digestive diverticula (p = 0.220). But strong positive correlation was found between adductor muscle and ovary (r = 0.450, p = 0.013). ANOVA showed that there were significant differences in total lipid and glycogen contents among months for all of the adductor muscle, ovary, and digestive diverticula (p < 0.05). The monthly changes in total lipid content were highly variable in ovary and digestive gland. High contents of total lipid were found during April and May-June in ovary, while March and June-July in digestive diverticula. There was a strong negative correlation in total lipid content between ovary and digestive diverticula (r = -0.397, p = 0.030). Unlike total protein or total lipid, glycogen content in the adductor muscle was most dynamic. It showed more than 36-fold changes in the adductor muscle (at most 3-fold change in ovary) during the study period. Glycogen content was higher during May-July in the adductor muscle, while it was higher in March and August in digestive diverticula. There was a strong negative correlation in glycogen content between the adductor muscle and digestive diverticula (r = -0.584, p = 0.001).

• Journal of Aquaculture
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• v.7 no.2
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• pp.109-122
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• 1994

The cultured olive flounder larvae given nutrient enriched rotifer, Artemia nauplii and commertial micro diet were investigated the changes of lipid content, fatty acid composition and vitamin E content in whole body with growth stages in order to compare with those of the wild one. The results obtained are as follows. 1) Lipid and vitamin E contents of the cultured larvae were higher than those of the wild one. 2) Clear differences of fatty acid composition with growth stages, especially 18: 2n-6 and 22 : 6n-3, were recognized between the wild and cultured olive flounder larvae. 3) Changes of lipid content, fatty acid composition and vitamin E content were found to be affected by dietary composition given to fish. 4) The wild olive flounder larvae were found to obtain natural live foods containing of low lipid content and high concentration 22: 6n-3 based on the fatty acid composition of the fish.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.43 no.6
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• pp.589-597
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• 2010

Monthly changes in the proximate and fatty acid compositions of chub mackerel (Scomber japonicus) muscle during cultivation from October 2007 to September 2008 were investigated. The lipid content increased gradually from the first stage of cultivation until March 2008 and then dramatically until May, before decreasing. The highest lipid content during cultivation was 21.6% in May, just before the fish spawns. There was a negative correlation (y=-1.1585x+87.741, $R^2$=0.9495) between the lipid and moisture contents during cultivation of chub mackerel. By contrast, the protein ($18.6{\pm}1.05%$) and ash ($1.18{\pm}0.11%$) contents were essentially unchanged during cultivation. Prominent fatty acids in chub mackerel muscle were 16:0, 18:0, 14:0 saturates, 18:1n-9, 16:1n-7, 18:1n-7 monoenes, and 22:6n-3 (docosahexaenoic acid, DHA), 20:5n-3 (eicosapentaenoic acid, EPA), and 18:2n-6 polyenes. The percentages of n-3 polyunsaturated fatty acids (PUFA), such as DHA and EPA, were higher during three months in the early stage of cultivation than they were subsequently. However, the PUFA (DHA+EPA) content (in mg/100 g of muscle) was lower in the early stage (740-796 mg/100 g muscle) than in the other stages. The highest PUFA (DHA+EPA) content was from April to May (2,749-2751 mg/100 g muscle). The PUFA content was positively correlated with the total lipid content of chub mackerel muscle during cultivation. The results indicate that cultured chub mackerel is a very good source of n-3 PUFA, such as DHA and EPA.

• Korean Journal of Food Science and Technology
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• v.17 no.4
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• pp.271-275
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• 1985

This investigation was conducted to observe changes of lipid components (phospholipids, free sterols, free fatty acids, triglycerides and estrified sterols) and fatty composition in diethyl ether extract and 86% MeOH extract from cotyledon and seedling axis of mung bean which were germinated in the dark at 25-$27^{\circ}C$ for 7 days. The total lipid contents in cotyledonn and seedling axis gradually decreased during germination. The triglyceride content in total lipid from cotyledon decreased and free fatty acid content increased, but triglyceride content from seedling axis decreased slightly and free fatty acid content decreased. Free sterol, esterified sterol and phospholipid content in cotyledon increased continuously, but their content in seedling axis dcreased slightly. The major fatty acids in mung bean were palmitic acid, linoleic acid and linolenic acid.

• The Korean Journal of Pharmacology
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• v.16 no.2 s.27
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• pp.31-38
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• 1980

Calvert et al. formulated the hypothesis that carbon tetrachloride ($CCl_4$) acted on the central nervous system to produce and intensify sympathetic discharge which resulted in anoxic necrosis of the liver. Recknagel suggested that the essential feature of $CCl_4$ hepatotoxicity depended on the cleavage of it to $CCl_3$(free radical) and the peroxidative decomposition of cytoplasmic membrane structural lipids. And there are many reports which show the increase of adrenergic activity in hyperthyroidism. In this paper, the influence of thyroxine on the hepatotexicity of carbon tetrachloride was investigated in mice. The results obtained were summarized as follows; 1) Hepatic total lipid and lipid peroxide contents were slightly decreased by L-sodium thyroxine injection(4mg/kg/day for 4days or 6days), but hepatic glycogen content was significantly decreased. 2) Hepatic total lipid and lipid peroxide contents and serum lactic dehydrogenase activity were significantly increased by $CCl_4$ (4 ml/kg single dose or triple dose: 4ml/kg/day for 3days), but hepatic glycogen content was significantly decreased. 3) The increase of hepatic total lipid and lipid peroxide contents and serum lactic dehydrogenase activity induced by $CCl_4$ were significantly inhitited by the pretreatment of thyroxine. 4) The decrease of hepatic glycogen induced by $CCl_4$ was not affected by the pretreatment of thyroxine.

• KSBB Journal
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• v.25 no.4
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• pp.363-370
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• 2010

Lipid from Scenedesmus sp. was extracted, fractionated and purified by silicic acid column chromatography. Total lipid content of extract was $21.38{\pm}1.03$ wt%, and triacylglycerol, an index lipid for biodiesel production was detected. Ten species of $C_{16}{\sim}C_{22}$ with saturated and unsaturated fatty acid were identified showing the very adequate fatty acid profiles for biodiesel production with a good flow property at low temperature. The fractions of glycolipid, neutral and phospholipid were 52.64, 28.10 and 19.26% of total lipid, respectively. The triacylglycerol of 12.63% and chlorophyll a of 49.47% was fractioned using stepwise elution of n-hexane-diethyl ether (95:5, v/v) solvent. The high content of chlorophyll was considered as a potential source of value-added co-product.

• Journal of the Korean Applied Science and Technology
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• v.4 no.2
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• pp.31-34
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• 1987

Effect of initial PH on the lipid accumulation and fatty acid composition of some species of mold were investigated. The maximum lipid content content produced by Aspergillus niger var. macrosporus was 17.5% at PH 3.5, by Aspergillus fumigatus 23.5% at PH 3.5 by Penicillium spinulosum 12.0% at PH 4.0 and by Penicillium notatum 7.3% at PH 4.0. The major fatty acids were palmitic, stearic, oleic and linoleic acid in all experimental molds. At PH from 4.5 to 6.0, the proportion of linoleic acid was increased and those of palmitic and oleic acid were decreased with rising in PH, therefore, degree of lipid unsaturation was increased in all experimental molds.