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Extraction, Purification and Property of the lipid from Scenedesmus sp.  

Kim, Na-Young (Department of Bioengineering and Technology, Kangwon National University)
Oh, Sung-Ho (School of Biotechnology and Bioengineering, Kangwon National University)
Choi, Woon-Yong (School of Biotechnology and Bioengineering, Kangwon National University)
Lee, Hyeon-Yong (School of Biotechnology and Bioengineering, Kangwon National University)
Lee, Shin-Young (Department of Bioengineering and Technology, Kangwon National University)
Publication Information
KSBB Journal / v.25, no.4, 2010 , pp. 363-370 More about this Journal
Abstract
Lipid from Scenedesmus sp. was extracted, fractionated and purified by silicic acid column chromatography. Total lipid content of extract was $21.38{\pm}1.03$ wt%, and triacylglycerol, an index lipid for biodiesel production was detected. Ten species of $C_{16}{\sim}C_{22}$ with saturated and unsaturated fatty acid were identified showing the very adequate fatty acid profiles for biodiesel production with a good flow property at low temperature. The fractions of glycolipid, neutral and phospholipid were 52.64, 28.10 and 19.26% of total lipid, respectively. The triacylglycerol of 12.63% and chlorophyll a of 49.47% was fractioned using stepwise elution of n-hexane-diethyl ether (95:5, v/v) solvent. The high content of chlorophyll was considered as a potential source of value-added co-product.
Keywords
Scenedesmus sp.; lipid; solvent extract; fractionation; property;
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