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Hydrolysis by Alcalase Improves Hypoallergenic Properties of Goat Milk Protein

  • Jung, Tae-Hwan;Yun, Sung-Seob;Lee, Won-Jae;Kim, Jin-Wook;Ha, Ho-Kyung;Yoo, Michelle;Hwang, Hyo-Jeong;Jeon, Woo-Min;Han, Kyoung-Sik
    • Food Science of Animal Resources
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    • v.36 no.4
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    • pp.516-522
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    • 2016
  • Goat milk is highly nutritious and is consumed in many countries, but the development of functional foods from goat milk has been slow compared to that for other types of milk. The aim of this study was to develop a goat milk protein hydrolysate (GMPH) with enhanced digestibility and better hypoallergenic properties in comparison with other protein sources such as ovalbumin and soy protein. Goat milk protein was digested with four commercial food-grade proteases (separately) under various conditions to achieve the best hydrolysis of αs -casein and β-lactoglobulin. It was shown that treatment with alcalase (0.4%, 60℃ for 30 min) effectively degraded these two proteins, as determined by SDS-PAGE, measurement of nonprotein nitrogen content, and reverse-phase high-performance liquid chromatography. Hydrolysis with alcalase resulted in a significant decrease in β-lactoglobulin concentration (almost to nil) and a ~40% reduction in the level of αs-casein. Quantification of histamine and TNF-α released from HMC-1 cells (human mast cell line) showed that the GMPH did not induce an allergic response when compared to the control. Hence, the GMPH may be useful for development of novel foods for infants, the elderly, and convalescent patients, to replace cow milk.

Use of Complementary and Alternative Medicine in Patients with Gynecologic Cancer: a Systematic Review

  • Akpunar, Dercan;Bebis, Hatice;Yavan, Tulay
    • Asian Pacific Journal of Cancer Prevention
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    • v.16 no.17
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    • pp.7847-7852
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    • 2015
  • Purpose: Research carried out with gynecologic cancer patients using CAM was reviewed to provide a source for discussing which CAM method is used for which purpose, patients' perceptions on the effects/side effects occurred during/after using CAM and their sources of information regarding CAM. Materials and Methods: This literature review was carried out for the period between January 2000 and March 2015 using Scopus, Dynamed, Med-Line, Science Dırect, Ulakbim, Research Starters, Ebscohost, Cinahl Complete, Academic Onefile, Directory of Open Access Journals, BMJ Online Journals (2007-2009), Ovid, Oxford Journal, Proquest Hospital Collection, Springer-Kluwer Link, Taylor & Francis, Up To Date, Web Of Science (Citation Index), Wiley Cochrane-Evidence Base, Wiley Online Library, and Pub-Med search databases with "complementary and alternative medicine, gynecologic cancer" as keywords. After searching through these results, a total of 12 full length papers in English were included. Results: CAM use in gynecologic cancer patients was discussed in 8 studies and CAM use in breast and gynecologic cancer patients in 4. It was determined that the frequency of CAM use varies between 40.3% and 94.7%. As the CAM method, herbal medicines, vitamins/minerals were used most frequently in 8 of the studies. When the reasons why gynecologic cancer patients use CAM are examined, it is determined that they generally use to strengthen the immune system, reduce the side effects of cancer treatment and for physical and psychological relaxation. In this review, most of the gynecologic cancer patients perceived use of CAM as beneficial. Conclusions: In order that the patients obtain adequate reliable information about CAM and avoid practices which may harm the efficiency of medical treatment, it is recommended that "Healthcare Professionals" develop a common language.

D-Pinitol Promotes Apoptosis in MCF-7 Cells via Induction of p53 and Bax and Inhibition of Bcl-2 and NF-κB

  • Rengarajan, Thamaraiselvan;Nandakumar, Natarajan;Rajendran, Peramaiyan;Haribabu, Lingaiah;Nishigaki, Ikuo;Balasubramanian, Maruthaiveeran Periyasamy
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.4
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    • pp.1757-1762
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    • 2014
  • Development of drugs from natural products has been undergoing a gradual evoluation. Many plant derived compounds have excellent therapeutic potential against various human ailments. They are important sources especially for anticancer agents. A number of promising new agents are in clinical development based on their selective molecular targets in the field of oncology. D-pinitol is a naturally occurring compound derived from soy which has significant pharmacological activitites. Therefore we selected D-pinitol in order to evaluate apoptotic potential in the MCF-7 cell line. Human breast cancer cells were treated with different concentrations of D-pinitol and cytotoxicity was measured by MTT and LDH assays. The mechanism of apoptosis was studied with reference to expression of p53, Bcl-2, Bax and NF-kB proteins. The results revealed that D-pinitol significantly inhibited the proliferation of MCF-7 cells in a concentration-dependent manner, while upregulating the expression of p53, Bax and down regulating Bcl-2 and NF-kB. Thus the results obtained in this study clearly vindicated that D-pinitol induces apotosis in MCF-7 cells through regulation of proteins of pro- and anti-apoptotic cascades.

Clothing education of domestic and foreign specialized high schools (국내외 의상 관련 고등학교의 교과교육 비교)

  • Yoo, Hye-Ja;Lee, Young-Suk
    • The Research Journal of the Costume Culture
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    • v.22 no.4
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    • pp.619-631
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    • 2014
  • This study proposes an educational direction to be followed by the clothing departments of Korea's specialized high schools in order to help achieve high levels of job placements and to strengthen their students' competitiveness. A comparison was made of the curricula in Korea's specialized high schools and in several fashion schools in other countries including the United States, Italy, France, Taiwan, and Japan. To this end, publicly available educational records were retrieved from domestic school documentation, vocational high school portals, and domestic/overseas school web sites, and were then reviewed. The archival data extracted from these sources shows that the curricula of the three chosen domestic schools uniformly focus on foundational courses such as general computer skills, general design, accounting principles, design principles, and drawing, as well as practical courses such as the design of western clothing, fashion design, fashion CAD, management of clothing material, design of Korean clothing, and knitting. Unlike these standardized courses, it was found that overseas fashion schools provide a more technically-advanced and design-focused education based on the interests of the individual student. Moreover, their education system nurtures the students' creativity through adequate field experience and history/language education. The findings of this study suggest that Korean specialized high schools should find a way to accommodate their students' interests and to provide them with personalized fashion education by adopting a more flexible curriculum. An educational line that considers the individual student's aptitudes and career path will help foster creativity and novel ideas, which, in turn, will contribute to the development of the fashion industry.

Educational Algorithm to train Renewable Energy Technician (신재생에너지 전문기술 인력양성 교육 알고리즘)

  • Yoon, Yongho
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.20 no.2
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    • pp.271-276
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    • 2020
  • In terms of supply of manpower in the energy industry, domestic universities cannot realistically operate flexibly to meet manpower demand in accordance with various regulations related to the department, and there are only a few independent energy related departments. There are many difficulties in closure, increase and decrease of capacity, and foster convergence. In addition to the limited aspects of human resource development of universities, changes in the energy paradigm, namely: 1) changes in the energy industry from stable supply and energy security to new growth engines and export industries, 2) Revitalization of the energy industry, 3) including diversification of energy sources and the spread of new technologies. Therefore, in this paper, we propose a training program for nurturing new renewable energy specialists to independently develop various high-quality human resources such as intangible and convergent human resources in line with changes in the energy paradigm.

The black hole mass-stellar velocity relation of the present-day active galaxies

  • Woo, Jong-Hak
    • The Bulletin of The Korean Astronomical Society
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    • v.35 no.1
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    • pp.79-79
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    • 2010
  • To investigate whether the present-day active galaxies follow the same black hole mass vs. stellar velocity dispersion (MBH-$\sigma*$) relation as quiescent galaxies, we measured the velocity dispersions of a sample of local Seyfert 1 galaxies, for which black hole masses were measured via reverberation mapping. We measured stellar velocity dispersions from high S/N optical spectra centered on the Ca II triplet region (${\sim}8500^{\circ}A$), obtained at the Keck, Palomar, and Lick Observatories. For two objects, in which the Ca II triplet region was contaminated by nuclear emission, we used high-quality H-band spectra obtained with the OH-Suppressing Infrared Imaging Spectrograph and laser-guide star adaptive optics at the Keck-II Telescope. Combining our new measurements with data from the literature, we assemble a sample of 24 active galaxies with stellar velocity dispersions and reverberation MBH in the range of black hole mass 106< MBH /$M{\odot}$ < 109,toobtainthefirstreverberationmappingconstraintsontheslopeandintrinsicscatteroftheMBH- $\sigma*$ relation of active galaxies. Assuming a constant virial coefficient f for the reverberation MBH, we find a slope ${\beta}=3.55{\pm}0.60$ and the intrinsic scatter ${\sigma}int=0.43{\pm}0.08$ dex in the relation log (MBH/M${\odot}$)=$\alpha+\beta$ log(${\sigma}*$/200 km s-1), which are consistent with those found for quiescent galaxies. We derive an updated value of the virial coefficient f by finding the value which places the reverberation masses in best agreement with the MBH - $\sigma*$ relation of quiescent galaxies; using the quiescent MBH - $\sigma*$ relation determined by Gultekin et al. we find log f=0.72+0.09 (or $0.71{\pm}0.10$) with an intrinsic scatter of $0.44{\pm}0.07$ (or 0.46+0.07) dex. No correlations between f and parameters connected to the physics of accretion (such as the Eddington ratio or line-shape measurements) are found. The uncertainty of the virial coefficient remains one of the main sources of the uncertainty in black hole mass determination using reverberation mapping, and therefore also in single-epoch spectroscopic estimates of black hole masses in active galaxies.

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Selection of Tropane Alkaloids High-Producing Lines by Single Cell Cloning of Hyoscyamus niger L. Root Cultures

  • Min, Ji-Yun;Park, Dong-Jin;Jeong, Mi-Jin;Song, Hyun-Jin;Kang, Seung-Mi;Kang, Young-Min;Choi, Myung-Suk
    • Journal of Korean Society of Forest Science
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    • v.98 no.2
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    • pp.142-147
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    • 2009
  • Hyoscyamus species is sources of the hypnotic and sedative drugs hyoscyamine and scopolamine. Single cells of Hyoscyamus niger were dissociated from suspension cultures and adventitious roots obtained from single-cell clones which were cultured on B5 medium containing 3% (w/v) sucrose, 0.1 mg/L IBA and 0.4% (w/v) gelrite. H. niger adventitious root lines showed wide variation in tropane alkaloids production and growth. An effective selection of 200 root lines was made possible by the application of the 'Dragendorff's reagent' for qualitative detection of the alkaloids from root. A high correlation coefficient (r=0.9390) was observed between the values obtained with the two methods based on HPLC and Dragendorff's reagent analysis. Among the selected roots, the highest scopolamine content was 16.64 mg/g DW (Hn-59), which was 8.82-fold more productive than the lowest alkaloid producing line (Hn-25). Here, we established an efficient selection method on tropane alkaloids production and suggest that the Dragendorff's reagent is of great practical value in selection of invisible compounds.

The Facade Decoration of Julio Romano's House in Mantua: Ideas on Painting Expressed through Mercury (줄리오 로마노의 만토바 저택: 메르쿠리우스로 표현된 화가의식)

  • Lee, Hansoon
    • The Journal of Art Theory & Practice
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    • no.14
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    • pp.159-186
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    • 2012
  • Julio Romano decorated the facade of his house in Mantua with a statue of Mercury to give expressions to his ideas on painting. Hereby the painter from Rome could show his home to the world as that of a painter. To be concrete, Mercury was the planet god to which visual artists belonged, and so was basically related to visual arts. In his role to deliver diverse features of art works Mercury could also convey concepts and emotions expressed in a picture to the viewer. The power of a painting to arouse certain emotions or move the mind of the viewer was further connected to the role of Mercury as the guide of the human soul. This function again related the Roman god to the characteristic of a portrait to present absent persons to the viewer. Above the statue of Mercury, a Lucian head of the god is seen, so that they together form the central axis of the facade. This seems to emphasize that the theme of the facade decoration was the powerful persuasive forces of eloquence. The two masks on the left could then refer to sources of eloquence, I.e. various beautiful expressions of a language and its generative process. On the other hand, the masks on the right could represent consequences of eloquence, for instance, prudence, evil effects which come about to imprudent listeners, and other influences on listeners. Finally, it would be useful to remind us of a line from On Architecture by Leon Battista Alberti. According to the humanist architect parts of a building which are seen from the outside, like a facade, should be appropriately designed, since the decoration of a house could play a significant role to enhance the fame and honor of the family and its fatherland. This theory of Alberti could have provided the foundation to the facade decoration of the Casa Pippi which proudly presented the profession of painting to the public in visual form.

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A comparative analysis of reference education between the United States and Korea (미국과 한국의 참고봉사 교육의 비교분석)

  • 정춘화
    • Journal of Korean Library and Information Science Society
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    • v.23
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    • pp.253-284
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    • 1995
  • The purpose of this paper is to analyse the current status of reference education and to suggest directions for reference education in America and in Korea. For this purpose, the historical developments of reference services, and the names, problems and changes of reference courses are studied. The findings of the study can be summarized as follows; 1. The names of reference courses are various. In America, On-Line Services or Information Services are used in general, but in Korea, Reference Services are widely used. 2. In America, some library schools have only subject literature courses including business, law, music, etc. instead of basic reference courses. 3. Only one reference course is given by most schools in America and in Korea. However, a few schools which have no graduate courses provide two reference courses in Korea. 4. Analysis of textbooks used in reference courses shows that Reference Research by Joon-Shik Park, Reference Services and Reference Sources by Ock-Soon Noh are used in general. In addition, Introduction to Reference Work by Katz is used. 5. Lecture methods are generally used in teaching reference courses, but reviews of reference materials in library, class presentation, and case study method are also used. It is desirable that role playing and pathfinders' used in U.S. are introduced to our schools. 6. Analysis of library user instruction courses in Korea shows that 7 of 11 universities have the courses,, only one university teaches library user instruction as a part of reference courses, 3 universities don't. 7. Analysis of opinions about the directions for reference education shows that changes of reference courses names, expansion of courses contents, emphasis on communication technique, changes of teaching methods, proper combination of theory vs. practice, an increase in electronic reference education and training of subject specialist are needed.

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High-Speed Satellite Detection in High-Resolution Image Using Image Processing (영상 처리를 이용한 고해상도 영상 내 위성의 고속 검출)

  • Shin, Seunghyeok;Lee, Jongmin;Lee, Sangwook;Yang, Taeseok;Kim, Whoi-Yul
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.46 no.5
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    • pp.427-435
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    • 2018
  • Many countries are trying to deploy satellite surveillance systems for their national defense, and one of these system uses optical systems to observe the satellites above their territories. The optical satellite surveillance system requires the coordinates of the satellites in an acquired image and expects that those coordinates to be delivered to the tracking system. The proposed method detects the satellite sources in a high-resolution image with fast image processing for the optical surveillance system. To achieve faster detection, the proposed method reduces the size of the original image and approximates the trajectory of a satellite, so image processing methods are only applied to the nearby area of the approximated trajectory in the original image. The proposed method shows the similar detection performance faster than the previous method.