• Title/Summary/Keyword: light stress

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Measurement of red blood cell aggregation by analysis of light transmission in a pressure-driven slit flow system

  • Shin, S.;Park, M.S.;Jang, J.H.;Ky, Y.H.;Suh, J.S.
    • Korea-Australia Rheology Journal
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    • v.16 no.3
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    • pp.129-134
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    • 2004
  • The aggregation characteristics of red blood cells (RBCs) were measured using a newly developed light-transmission slit rheometer. Conventional methods of RBC disaggregation such as the rotational Couette system were replaced with a pressure-driven slit flow system with a vibrational mechanism. Using a vibration generator, one can disaggregate the RBC aggregates stored in the slit. While shear stress decreases exponentially, instantaneous pressure and the transmitted light intensity were measured over time. Applying an abrupt shearing flow after disaggregation caused a rapid elongation of the RBCs followed by loss of elongation with the decreasing shear stress. While the shear stress is further decreasing, the RBCs start to re-aggregate and the corresponding transmitted intensity increases with time, from which the aggregation indices can be obtained using a curve-fitting program.

The Effect of Light and Darkness on Acclimatization of Laying Hens

  • Izzeldin, B.;Kassim, H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.13 no.5
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    • pp.694-697
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    • 2000
  • Laying hens kept in different light and dark periods of the day at high ambient temperature of maximum $35^{\circ}C$ were challenged to $38.5{\pm}0.5^{\circ}C$ acute heat 3 hours daily for 7 consecutive days. They were found to have a significant (p<0.01) acclimatization response (rectal temperature) to heat stress during the dark period compared to those exposed to the same temperature during the light period. The blood pH was not significantly different. The partial pressure of carbon dioxide ($PCO_2$) was significantly high (p<0.01) except in day 4. Similarly the blood bicarbonate ($HCO_3$) concentration was significantly high (p<0.05) except day three and day four. Acute heat exposure in the first day increased the body temperature in both groups (Light and Dark) reaching $44^{\circ}C$, followed by gradual reduction in body temperature. The dark treated birds showed rapid reduction in body temperature ($42.88^{\circ}C$) and adaptation to high temperature during days 2-4 but that this was lost to some extent in days 6-8. However this was not obvious in the light treated birds. It is concluded that darkness reduce hyperthermia and enhance acclimatization responses during acute heat stress.

A Rhythmic Effect of Tone in English (영어 억양의 리듬효과)

  • Lee, Joo-Kyeong;Kang, Sun-Mi
    • Speech Sciences
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    • v.10 no.2
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    • pp.303-318
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    • 2003
  • This paper attempts to investigate the tonal implementations of English stress clash, arguing that a preceding stress shifts leftward when two lexical stresses conflict across word boundaries or that H* and L* pitch accents are alternatively manifested on the stressed syllables, establishing intonational peak and valley contours. We claim that the H*/L* alternation might be a tonal strategy to avoid stress clash, and that pitch could be solely manipulated to display a rhythmic effect with maintaining lexical stress. In the experiment, we examined two-word combinations whose boundaries involve stress clash, and divided them into two categories. One has the preceding words involving a heavy syllable ahead of stress to guarantee the place for a shifting stress and the other, a light syllable, in which case stress shift is completely prevented. We analyzed the distribution of pitch accents in the word combinations, focusing on the pitch configurations in the cases where stress should not be shifted. Results show that approximately 50% of the stimuli show stress shift in the heavy syllable combinations of the preceding words; the preceding stress is moved leftward within the word. The other 50% and the light syllable combinations show various pitch accents patterns; H* and L* alternation, deaccentuation of either stressed syllable, or L-insertion between two H* pitch accents, etc. We interpret this as a rhythmic effect of tone to avoid stress clash and suggest that a true stress clash would be confined into cases without H*/L* contours.

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High Light-Induced Changes in the Activities of Antioxidant Enzymes and the Accumulation of Astaxanthin in the Green Alga Haematococcus pluvialis

  • Park, Seul-Ki;Jin, Eon-Seon;Lee, Choul-Gyun;Lee, Mi-Young
    • Molecular & Cellular Toxicology
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    • v.4 no.4
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    • pp.300-306
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    • 2008
  • We investigated high light-induced alterations in antioxidant enzymes by exposing green vegetative cells of the alga Haematococcus pluvialis to excess irradiance to induce the production of astaxanthin, a carotenoid pigment. Total activity of catalase decreased approximately 70% after high light exposure, whereas glutathione peroxidase (GPX) activity was slightly enhanced. Total activity of superoxide dismutase and ascorbate peroxidase (APX) also slightly decreased. Overall, we did not observe dramatically elevated levels of antioxidant isozymes, although APXn, GPX2, and GPX3 isozyme increased slightly. ${H_2}{O_2}$ content increased about sixfold after high light exposure, demonstrating severe cellular oxidative stress, whereas lipid peroxidation was notably reduced. Concomitantly, astaxanthin accumulation increased about sevenfold. This result suggests that probably massively accumulated astaxanthin may be one of the antioxidant protector against high light stress.

Independent Control of Wrinkle Wavelength and Height for Optoelectronic Devices via Changing Stress Relaxation Time (응력 해소 시간 변화를 통한 광전자소자용 주름구조 주기와 높이의 독립적 제어 연구)

  • Gu, Bongjun;Kim, Jongbok
    • Journal of Adhesion and Interface
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    • v.23 no.2
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    • pp.39-43
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    • 2022
  • In optoelectronic devices including displays and solar cells that convert electricity into light or light into electricity, it is important to control optical behavior of light to improve device efficiency. Specifically, the control of internal emitting light in the OLEDs can induce more light to go out, improving luminous efficiency. In addition, the control of optical behavior of incident light in solar cells can increase optical path in the light absorption layer, increasing power-conversion efficiency. In this study, we generated wrinkles as a physical structure to control optical behavior of light and independently controlled their wavelength and height by changing stress relaxation time. To explore the effect of wavelength and height on optical behavior, we conducted UV/Vis spectroscopy analysis of wrinkles with various heights at a constant wavelength or various wavelengths at a comparable height, figuring out a wrinkle with high aspect ratio has more dispersive light and less straight light. It indicates that high aspect ratio is required to change the optical behavior and increase the optical path.

Isolation and Characterization of Aspergillus nidulans Mutants Which Undergo Sexual Development in Light Exposure (빛의 존재하에서도 유성분화를 하는 Aspergillus nidulans의 돌연변이체 분리 및 분석)

  • Min, Jung-Youl;Kim, Hye-Ryun;Han, Kap-Hoon;Han, Dong-Min
    • Korean Journal of Microbiology
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    • v.43 no.2
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    • pp.77-82
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    • 2007
  • In a homothallic ascomycete Aspergillus nidulans, sexual development is inhibited by various environmental stresses such as acetate medium, visible light and high osmolarity conditions. In order to study the genes involved in this stress-related regulatory network, we first attempted to isolate mutants that could develop cleistothecia even in the presence of any of those stresses including intensive visible light. More than 10,000 mutants were screened and 167 mutants were analyzed. Among them, 152 mutants underwent sexual development under the single stress condition of either high osmotic, high acetate or light condition but no sexual development in more than two stresses. Six mutants can produce cleistothecia under light or acetate stress but not in salt stress. Moreover, 6 mutants showed the ability to develop cleistothecia under the light but not under the acetate or osmo-stress. The mutants were revealed to have independent single gene mutation and grouped into different complementation groups (silA-F). The mutant alleles were all recessive to that of wild type. The light responsiveness of development implies the existence of delicate regulation process including reception and translocation of light signaling and determination of development.

Effects of Light Therapy on Stress, Depression, Quality of Sleep and Quality of Life in Middle-Aged Women: A Randomized Controlled Trial (빛 요법이 중년여성의 스트레스, 우울, 수면의 질 및 삶의 질에 미치는 효과: 무작위 대조군 연구)

  • Jang, Mi-Kyeong;Hur, Myung-Haeng
    • Journal of the Korea Convergence Society
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    • v.12 no.7
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    • pp.317-327
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    • 2021
  • This study is a randomized controlled trial to compare the effects of light therapy on stress, depression, sleep quality and quality of life in middle-aged women. The participants of this study were 56 middle-aged women who worked at the same office. Data collection period was from August 26 to September 13 for the control group and from September 16 to October 4 for the experimental group. Subjective stress, depression, quality of sleep and quality of life in middle-aged women were measured by Numeric Rating Scale (NRS), Center for Epidemiology studies of scale (CES-D), Verran & Synder-Halpern Sleep scale (VHS) and WHO Quality of Life Scale Abbreviated Version (WHOQOL-BREF). Stress index was measured using a Canopy9 RSA (IEMBIO, Gangwondo, Korea) equipment. The data were analyzed by mean, standard deviation, real number and percentage, χ2-test, t-test, repeated measures ANOVA and ANCOVA using IBM SPSS Statistics version 25.0. The results showed that the experimental group applying light therapy had significantly lower subjective stress (F=6.30, p<.001) and higher quality of life (F=2.80, p=.049) than the control group, There was no difference in objective stress index, depression, and sleep quality. As a result, light therapy is partially effective in subjective stress and quality of life for middle-aged women, and further research is needed on middle-aged women working in work environments with limited light exposure.

EFFECT OF SOFT-START CURING ON THE CONTRACTION STRESS OF COMPOSITE RESIN RESTORATION POLYMERIZED WITH LED AND PLASMA CURING UNIT (LED와 플라즈마 광원의 완속기시 광중합 방식이 복합레진의 수축응력에 미치는 영향)

  • Chung, Yang-Seok;Lee, Nan-Young;Lee, Sang-Ho
    • Journal of the korean academy of Pediatric Dentistry
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    • v.34 no.4
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    • pp.623-631
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    • 2007
  • Effect of Soft-start curing on the contraction stress of composite resin restoration polymerized with LED and plasma curing unit The purpose of this study was to evaluate the influence of soft-start light curing on contraction stress and hardness of composite resin. Composite resin (Filtek $Z-250^{TM}$, 3M ESPE, USA) was cured using the one-step continuous curing method with three difference light sources ; conventional halogen light ($XL3000^{TM}$, 3M ESPE, USA) cure for 40 seconds at $400 mw/cm^2$, LED light (Elipar Freelight $2^{TM}$, 3M-ESPE, USA) cure for 20 seconds at $800\;mW/cm^2$ a and plasma arc light ($Flipo^{TM}$, LOKKI, France) cure for 12 seconds at $1300 mW/cm^2$. For the soft-start curing method ; LED light (Elipar Freelight $2^{TM}$, 3M-ESPE, USA) cure exponential increase with 5 seconds followed by 17 seconds at $800\;mW/cm^2$ and plasma arc light ($Flipo^{TM}$, LOKKI, France) cure 2 seconds light exposure at $650\;mW/cm^2$ followed by 11 seconds at $1300\;mW/cm^2$. The strain guage method was used for determination of polymerization contraction. Measurements were recorded at each 2 second for the total of 800 seconds including the periods of light application. Obtained data were analyzed statically using Repeated measures ANOVA, One way ANOVA, and Tukey test. The results of present study can be summarized as follows: 1. Composite resin restoration showed transient expansion just after irradiation of curing light. Contraction stress was increased rapidly at the early phase of polymerization and reduced slowly as time elapsed (P<0.05). 2. Contraction stress was not revealed significant difference between Halogen curing light groups and LED and Plasma Light curing with soft-start group (P>0.05). 3. LED and Plasma Light curing with soft-start showed lower contraction stress than the one-step continuous light curing (P<0.05).

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Inhibition of Cell Growth and Mitochondrial Activity in Human Gingival Fibroblasts by LED-Generated Red Light Exposure

  • Kim, Hee-Jeong;Hwang, Jung-Min;Kwak, So-Yeong;Kim, Jong-Ghee;Jeon, Young-Mi;Lee, Jeong-Chae
    • International Journal of Oral Biology
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    • v.34 no.4
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    • pp.185-190
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    • 2009
  • This study examined the effects of red light generated from a light emitting diode (LED) upon proliferation and mitochondrial stress in human gingival fibroblasts (hGFs). Cells were exposed to LED-generated red light at a clinically relevant intensity and distance with a 610-630 nm wavelength for various times (0-48 min). At different exposure times, cells were processed for the analysis of succinate dehydrogenase (SDH) activity, proliferation, mitochondrial membrane potential (MMP) and cytotoxicity. Cell cycle progression was also investigated by flow cytometry after staining with propidium iodide. Red light exposure was found to inhibit SDH activity and DNA synthesis in hGFs in a time-dependent manner. Light exposure also reduced the MMP levels in these cells and this was closely associated with a $G_0/G_1$ arrest. In contrast, exposure of hGFs to red light for 48 min led to a dramatic loss of MMP with an attendant increase in cytotoxicity. These findings demonstrate that LED-generated red light may cause mitochondrial stress and growth inhibition in hGFs during tooth whitening therapy, depending on the length of the exposure.

Stress analysis of the CR lens using the chrome conversion (Chrome 변환을 이용한 CR 렌즈의 미세응력 시각화)

  • Kim, Yong-Geun
    • Journal of Korean Ophthalmic Optics Society
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    • v.10 no.1
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    • pp.9-15
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    • 2005
  • The polariscope to measure the microscopic stress in CR lens consists of light source polarizer, model, polarizer, CCD, computer, chrome conversion orderly and the principal-stressed difference, (${\sigma}_1-{\sigma}_2$) and the fringe order n were measured by analyzing two components of light wave $E_1$ and $E_2$ following each polarizer's steps. The two-dimensional model could be determined from the fact that the optical axes of sample concide with the principal-stress directions. The bi-refringence acted to a light wave and the phase retardation were in proportion to the principal-stressed difference(${\sigma}_1-{\sigma}_2$) and the intensity of final light wave was proportioned to $sin2({\Delta}/2)$ and when ${\Delta}/2=n{\pi}$ (n=0, 1, 2, ${\ldots}$) the extinction occurs. Photoelastic's image by microscopic stress could analyzed using chrome conversion, and the image showed clearly.

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