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Isolation and Characterization of Aspergillus nidulans Mutants Which Undergo Sexual Development in Light Exposure  

Min, Jung-Youl (Div. Life Science, Wonkwang University)
Kim, Hye-Ryun (Div. Life Science, Wonkwang University)
Han, Kap-Hoon (Dept. Pharmaceutical Engineering, Woosuk University)
Han, Dong-Min (Div. Life Science, Wonkwang University)
Publication Information
Korean Journal of Microbiology / v.43, no.2, 2007 , pp. 77-82 More about this Journal
Abstract
In a homothallic ascomycete Aspergillus nidulans, sexual development is inhibited by various environmental stresses such as acetate medium, visible light and high osmolarity conditions. In order to study the genes involved in this stress-related regulatory network, we first attempted to isolate mutants that could develop cleistothecia even in the presence of any of those stresses including intensive visible light. More than 10,000 mutants were screened and 167 mutants were analyzed. Among them, 152 mutants underwent sexual development under the single stress condition of either high osmotic, high acetate or light condition but no sexual development in more than two stresses. Six mutants can produce cleistothecia under light or acetate stress but not in salt stress. Moreover, 6 mutants showed the ability to develop cleistothecia under the light but not under the acetate or osmo-stress. The mutants were revealed to have independent single gene mutation and grouped into different complementation groups (silA-F). The mutant alleles were all recessive to that of wild type. The light responsiveness of development implies the existence of delicate regulation process including reception and translocation of light signaling and determination of development.
Keywords
Aspergillus nidulans; environmental stress; sexual development; SIL mutants;
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