• Title/Summary/Keyword: light curing

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Biological Effects of Light-Emitting Diodes Curing Unit on MDPC-23 Cells and Lipopolysaccharide Stimulated MDPC-23 Cells

  • Jeong, Moon-Jin;Jeong, Soon-Jeong
    • Journal of dental hygiene science
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    • v.19 no.1
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    • pp.39-47
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    • 2019
  • Background: Light-emitting diodes curing unit (LCU), which emit blue light, is used for polymerization of composite resins in many dentistry. Although the use of LCU for light-cured composite resin polymerization is considered safe, it is still controversial whether it can directly or indirectly have harmful biological influences on oral tissues. The aim of this study was to elucidate the biological effects of LCU in wavelengths ranging from 440 to 490 nm, on the cell viability and secretion of inflammatory cytokines in MDPC-23 odontoblastic cells and inflammatory-induced MDPC-23 cells by lipopolysaccharide (LPS). Methods: The MTT assay and observation using microscope were performed on MDPC-23 cells to investigate the cell viability and cytotoxic effects on LCU irradiation. Results: MDPC-23 cells and LPS stimulated MDPC-23 cells were found to have no effects on cell viability and cell morphology in the LCU irradiation. Nitric oxide (NO) and prostaglandin $E_2$ which are the pro-inflammatory mediators, and interleukin-$1{\beta}$ and tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) which are the proinflammatory cytokines were significantly increased in MCPD-23 cells after LCU irradiation as time increased in comparison with the control. LCU irradiation has the potential to induce inflammation or biological damages in normal dental tissues, including MDPC-23 cells. Conclusion: Therefore, it is necessary to limit the use of LCU except for the appropriate dose and irradiation time. In addition, LCU irradiation of inflammatory-induced MDPC-23 cells by LPS was reduced the secretion of NO compared to the LPS alone treatment group and was significantly reduced the secretion of TNF-${\alpha}$ in all the time groups. Therefore, LCU application in LPS stimulated MDPC-23 odontoblastic cells has a photodynamic therapy like effect as well as inflammation relief.

Relationship between battery level and irradiance of light-curing units and their effects on the hardness of a bulk-fill composite resin

  • Fernanda Harumi Oku Prochnow ;Patricia Valeria Manozzo Kunz;Gisele Maria Correr;Marina da Rosa Kaizer;Carla Castiglia Gonzaga
    • Restorative Dentistry and Endodontics
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    • v.47 no.4
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    • pp.45.1-45.10
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    • 2022
  • Objectives: This study evaluated the relationship between the battery charge level and irradiance of light-emitting diode (LED) light-curing units (LCUs) and how these variables influence the Vickers hardness number (VHN) of a bulk-fill resin. Materials and Methods: Four LCUs were evaluated: Radii Plus (SDI), Radii-cal (SDI), Elipar Deep Cure (Filtek Bulk Fill, 3M Oral Care), and Poly Wireless (Kavo Kerr). Irradiance was measured using a radiometer every ten 20-second activations until the battery was discharged. Disks (4 mm thick) of a bulk-fill resin (Filtek Bulk Fill, 3M Oral Care) were prepared, and the VHN was determined on the top and bottom surfaces when light-cured with the LCUs with battery levels at 100%, 50% and 10%. Data were analyzed by 2-way analysis of variance, the Tukey's test, and Pearson correlations (α = 5%). Results: Elipar Deep Cure and Poly Wireless showed significant differences between the irradiance when the battery was fully charged versus discharged (10% battery level). Significant differences in irradiance were detected among all LCUs, within each battery condition tested. Hardness ratios below 80% were obtained for Radii-cal (10% battery level) and for Poly Wireless (50% and 10% battery levels). The battery level showed moderate and strong, but non-significant, positive correlations with the VHN and irradiance. Conclusions: Although the irradiance was different among LCUs, it decreased in half of the devices along with a reduction in battery level. In addition, the composite resin effectiveness of curing, measured by the hardness ratio, was reduced when the LCUs' battery was discharged.

Evaluation of marginal leakage of bulk fill flowable composite resin filling with different curing time using micro-computed tomography technology (Bulk fill 유동성 복합레진의 변연 누출에서 다른 중합시간의 영향에 대해 마이크로시티를 이용한 평가)

  • Kim, Eun-Ji;Lee, Kyu-Bok;Jin, Myoung-Uk
    • Journal of Dental Rehabilitation and Applied Science
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    • v.32 no.3
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    • pp.184-193
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    • 2016
  • Purpose: To evaluate marginal leakage of bulk fill flowable composite resin filling with different curing time by using microcomputed tomography technology. Materials and Methods: 30 previously extracted human molars were randomly divided into 6 groups based upon restorative system and different curing time. Class II cavities (vertical slot cavities) were prepared. An individual metallic matrix was used to build up the proximal wall. The SonicFill or SureFil SDR flow was inserted into the preparation by using 1 bulk increment, followed by light polymerization for different curing times. The different exposure times were 20, 40, and 60 seconds. All specimens were submitted to 5,000 thermal cycles for artificial aging. Micro-CT scanning was performed by using SkyScan 1272. One evaluator assessed microleakage of silver nitrated solution at the resin-dentin interface. The 3D image of each leakage around the restoration was reconstructed with CT-Analyser V.1.14.4. The leakage was analyzed with the Mann-Whitney test. Results: Significant differences were observed between the light curing times, but no significant differences were found between the bulk fill composite resins. Increasing in the photoactivation time resulted in greater microleakage in all the experimental groups. Those subjected to 60 seconds of light curing showed higher microleakage means than those exposed for 20 seconds and 40 seconds. Conclusion: Increasing the photoactivation time is factor that may increase marginal microlekage of the bulk fill composite resins. Further, micro-CT can nondestructively detect leakage around the resin composite restoration in three dimensions.

Effects on the Tissue Reaction Using GI Cement in the Maxillary Grade II Furcation in the Beagle Dogs (성견 상악 치근 이개부 병소에 Glass Ionomer Cement 충전 시 조직 반응에 관한 연구)

  • Lee, Yong-Gon;Chung, Chin-Hyung;Lim, Sung-Bin
    • Journal of Periodontal and Implant Science
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    • v.30 no.4
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    • pp.793-803
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    • 2000
  • Procedures for treatment of molar furcation invasion defects range from open flap debridement, apically repositioned flap surgery, hemisection, tunneling or extraction, to regenerative therapies using bone grafting or guided tissue regenerative therapy, or a combination of both. Several clinical evaluations using regenerative techniques have reported the potential for osseous repair of treated furcation invasions. Regenerative treatment of maxillary molars are more difficult due to the multiple root anatomy and multiple furcation entrances therefore, purpose of this study was to evaluated histologically self-curing glass-ionomer cement and light-curing glass-ionomer cement as a barrier in the treatment of a bi-furcated maxillary premolar. Five adult beagle dogs were used in this experiment. With intrasulcular and crestal incision, mucoperiosteal flap was elevated. Following decortication with 1/2 high speed round bur, degree II furcation defect was made on maxillary third(P3), forth(P4) and fifth(P5) premolar. 2 month later experimental group were self-curing glassionomer cement and light-curing glassionomer cement. After 4, 8 weeks, the animals were sacrificed by vascular perfusion. Tissue block was excised including the tooth and prepared for light microscope with Gomori's trichrome staining. Results were as follows. 1. In all experiment group, there were not epithelial down growth and glass ionomer cement were encapsulated connective tissue. 2. In 4 weeks experiment I group slighly infiltrated inflammatory cells but not disturb the new bone or new cementum formation. 3. In 8 weeks, experiment groups I, II were encapsulated fine connective tissue. 4. Therefore glass-ionomer cement filling to the grade III maxillary furcations with multiple root anatomy and multiple furcation entrances were possible clinical methods and this technique is useful method for Maxillary furcation involvement.

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Effect of Shading Conditions on Curing of Burley Tobacco in Pipe Vinyl Houses (건조 House의 차광조건과 버어리종 담배건조)

  • Bae, S.K.;Choo, H.G.;Lim, H.G.;Ryu, C.H.;Han, C.S.
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.28 no.2
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    • pp.248-251
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    • 1983
  • The effects of shading - 90, 70, 50, 30, 10% on curing burley tobacco was investigated in pipe vinyl houses. The curing in sunlight under transparent materials resulted in an increase in temperature and a decrease in relative humidity. But amount of light on tobacco leaves were less than 10% sun light in all treatments. Shading was delayed curing period, but cured leaves under transparent shading materials were off color and brittle. Filling power and combustibility were deteriorated as decreased shading. But quality of cured leaves were non significant between treatments. Effects of shading were believed that resulted in a decrease in temperature and an increase in relative humidity than light.

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ELUTION OF RESIDUAL MONOMER ACCORDING TO VARIOUS LIGHT SOURCES AND CURING TIME ON THE POLYMERIZATION OF PHOTOACTIVATED PIT AND FISSURE SEALANTS (광중합 광원의 종류와 조사시간에 따른 치면열구전색제의 미반응 모노머 용출)

  • Oh, You-Hyang;Park, Yoon-Kyung;Lee, Nan-Young;Lee, Chang-Seop;Lee, Sang-Ho
    • Journal of the korean academy of Pediatric Dentistry
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    • v.31 no.3
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    • pp.421-430
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    • 2004
  • The purpose of this study was to measure and compare the amount of unreacted TEGDMA from pit and fissure sealants cured with three different light sources; conventional halogen light curing unit, plasma arc light curing unit and argon laser. The specimens were eluted in distilled water for different time intervals. The time-related release of TEGDMA were analyzed by reverse-phase high performance liquid chromatography(HPLC). The result of present study can be summarized as follows: 1. The time-related release of TEGDMA decreased with increasing curing time in conventional halogen light, however, that not statistically significant difference(p>0.05). 2. The elution from the specimens cured for 6 and 9 seconds with plasma arc light was similar results corresponding with the time-related TEGBMA release, and was significantly lower than that cured for 3 seconds(p<0.05). 3. The elution of TEGDMA from the specimens cured with argon laser was significantly higher than that cured with halogen and plasma arc light(p<0.05). 4. The elution of TEGDMA from under recommended time of three different light sources were showed to be no statistically significant difference(p>0.05). 5. In time-related release of TEGDMA from recommended time of each light sources, the results correspond to 40 seconds of halogen light and 6 seconds of plasma arc light were similar(p>0.05). 6. The elution of TEGDMA, from over recommended time of three different light sources were showed to be no statistically significant difference(p>0.05). In this study, I suggest that curing time of plasma arc light is 6 and/or 9 seconds in the field of clinical pediatric dentistry claiming its effectiveness in optimal polymerization and reduced chair time.

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A STUDY ON THE EVALUATION OF POLYMERIZATION SHRINKAGE OF COMPOSITE AND COMPOMER USING STRAIN GAUGE METHOD (스트레인 게이지법을 이용한 복합레진과 컴포머의 중합수축 평가에 관한 연구)

  • Kim, Yeun-Chul;Kim, Jong-Soo;Kwon, Soon-Won;Kim, Yong-Kee
    • Journal of the korean academy of Pediatric Dentistry
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    • v.29 no.1
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    • pp.19-29
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    • 2002
  • The purpose of this study was to compare the polymerization shrinkage and the compressive strength of composite and compomer cured with two different light sources ; conventional halogen-light curing unit and recently-developed plasma arc curing unit. The 'strain gauge method' was used for determination of polymerization shrinkage and the compressive strength was measured by universal testing machine. The results of the present study can be summarized as follows: 1. Filling materials in polyethylene molds showed the initial expansion in the early phase of polymerization. This was followed by the rapid contraction in volume during the first 60 seconds and gradually diminished as curing process continued. 2. The polymerization shrinkage in tooth samples was generally lower than in the mold samples. 3. The generally lower amount of linear polymerization shrinkage was observed in compomer and plasma arc curing unit group when compared to composite and conventional curing unit. 4. The higher compressive strength values was found in composite groups regardless curing methods. The results of this study strongly support the application of plasma arc system and fluoride-containing compomer in the field of clinical pediatric dentistry claiming its effectiveness in curing the esthetic dental materials and the anticariogenic capacity.

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Shear bond strength and adhesive failure pattern in bracket bonding with plasma arc light (Plasma arc light를 이용한 bracket 부착시의 전단결합강도와 파절양상의 유형)

  • Yoo, Hyung-Seok;Oh, Young-Geun;Lee, Seung-Yeon;Park, Young-Chel
    • The korean journal of orthodontics
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    • v.31 no.2 s.85
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    • pp.261-270
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    • 2001
  • The purpose of this study was to evaluate the clinical usefulness of plasma arc light which can reduce the curing time dramatically compared by shear bond strengths and failure patterns of the brackets bonded with visible light in direct bracket bonding. Some kinds of brackets were bonded with the Transbond$^{\circledR}$ to the human premolars which were embedded in the resin blocks according to the various conditions. After bonding, the shear bond strength was tested by Instron universal testing machine and in addition , the amount of residual adhesive remaining on the tooth after debonding was measured by the stereoscope and assessed with adhesive remnant index(ARI). The results were as follows : 1. When plasma arc light was used for bonding the brackets, the shear bond strength was clinically sufficient in both metal and ceramic brackets, but resin brackets showed significantly lower bond strength but which was clinically useful. 2. When metal brackets were bonded using visible light, there was no significant difference in shear bond strength due to the light-curing time and the bond strength was clinically sufficient. 3. When the adhesive failure patterns of brackets bonded with plasma arc light were observed by using the adhesive remnant index, the bond failure of the metal and resin bracket occurred more frequently at bracket-adhesive interface but the failure of the ceramic bracket occurred more frequently at enamel-adhesive interface. 4. There was no statistically significant difference of the shear bond strength and adhesive failure pattern between metal bracket bonded for 2 seconds by curing with plasma arc light and 10 seconds by curing with visible light. 6. When metal brackets were bonded using plasma arc light, the shear bond strength decreased as the distance from the light source increased. The above results suggest that plasma arc light can be clinically useful for bonding the brackets without fear of the decrease of the shear bond strength.

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Power density of light curing units through resin inlays fabricated with direct and indirect composites (직접수복용 레진과 기공용 레진으로 제작한 레진 인레이를 투과한 광중합기의 광강도)

  • Chang, Hoon-Sang;Lim, Young-Jun;Kim, Jeong-Mi;Hong, Sung-Ok
    • Restorative Dentistry and Endodontics
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    • v.35 no.5
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    • pp.353-358
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    • 2010
  • Objectives: The purpose of this study was to measure the power density of light curing units transmitted through resin inlays fabricated with direct composite (Filtek Z350, Filtek Supreme XT) and indirect composite (Sinfony). Materials and Methods: A3 shade of Z350, A3B and A3E shades of Supreme XT, and A3, E3, and T1 shades of Sinfony were used to fabricate the resin inlays in 1.5 mm thickness. The power density of a halogen light curing unit (Optilux 360) and an LED light curing unit (Elipar S10) through the fabricated resin inlays was measured with a hand held dental radiometer (Cure Rite). To investigate the effect of each composite layer consisting the resin inlays on light transmission, resin specimens of each shade were fabricated in 0.5 mm thickness and power density was measured through the resin specimens. Results: The power density through the resin inlays was lowest with the Z350 A3, followed by Supreme XT A3B and A3E. The power density was highest with Sinfony A3, E3, and T1 (p < 0.05). The power density through 0.5 mm thick resin specimens was lowest with dentin shades, Sinfony A3, Z350 A3, Supreme XT A3B, followed by enamel shades, Supreme XT A3E and Sinfony E3. The power density was highest with translucent shade, Sinfony T1 (p < 0.05). Conclusions: Using indirect lab composites with dentin, enamel, and translucent shades rather than direct composites with one or two shades could be advantageous in transmitting curing lights through resin inlays.