• 생명과학회지
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• 제25권7호
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• pp.819-825
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• 2015

본 연구는 과당 위주의 스포츠음료 투여가 최대운동부하시 심폐기능과 젖산 및 염증반응에 미치는 영향에 대해 비교 분석하기 위해 실시되었다. 연구대상자는 D 대학교 체육학과 학생 8명으로 구성하였으며 실험시기를 2차로 나누어 스포츠 음료 투여 전과 투여 후 시기에 심폐기능과 젖산 및 염증반응 등을 비교 분석하였다. 본 연구의 결과 스포츠 음료 섭취 전과 섭취후 운동시간과 V0₂max, AT HRmax가 유의하게 증가하였고(p<0.05), 스포츠 음료 투여 후 회복기 20분에서 젖산 농도가 유의하게 감소되었다(p<0.05). 따라서 고강도 최대운동 전 과당위주의 스포츠 음료 투여는 운동지속시간을 늘려주고 VO₂max를 높여주며, 운동 후 피로물질인 젖산축적의 빠른회복에 도움을 줄 수 있는 것으로 사료된다. 비록 본 연구에서 염증 반응에 대한 효과를 완벽하게 규명하지는 못했지만 스포츠 음료 투여시 CRP의 농도가 감소하는 경향이 나타나 염증반응 개선에도 어느 정도 영향을 미칠 수 있을 것이라 생각된다. 추후 과당위주의 스포츠 음료투여시 염증 반응에 대한 다양한 운동 강도와 상황에서의 기전적 연구가 수행되어져야 할 것이다.

• 통합자연과학논문집
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• 제4권2호
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• pp.149-157
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• 2011

Urbanization of the world's population has given rise to more than 450 cities around the world with populations in excess of 1 million (megacity) and more than 25 so-called metacities with populations over 10 million (Brinkhoff, 2010). The United States today has a total resident population of more than 308,500,000 people, with 81 percent residing in cities and suburbs as of mid - 2005 (UN, 2008). Urban meteorology is the study of the physics, dynamics, and chemistry of the interactions of Earth's atmosphere and the urban built environment, and the provision of meteorological services to the populations and institutions of metropolitan areas. While the details of such services are dependent on the location and the synoptic climatology of each city, there are common themes, such as enhancing quality of life and responding to emergencies. Experience elsewhere (e.g., Shanghai, Helsinki, Tokyo, Seoul, etc.) shows urban meteorological support is a key part of an integrated or multi-hazard warning system that considers the full range of environmental challenges and provides a unified response from municipal leaders. Urban meteorology has come to require much more than observing and forecasting the weather of our cities and metropolitan areas. Forecast improvement as a function of more and better observations of various kinds and as a function of model resolution, larger ensembles, predicted probability distributions; Responses of emergency managers, government officials, and users to improved and probabilistic forecasts; Benefits of improved forecasts in reduction of loss of life, property damage, and other adverse effects. A national initiative to enhance urban meteorological services is a high-priority need for a wide variety of stakeholders, including the general, commerce and industry, and all levels of government. Some of the activities of such an initiative include: conducting basic research and development; prototyping and other activities to enable very--short and short range predictions; supporting and improving productivity and efficiency in commercial and industrial sectors; and urban planning for long term sustainability. In addition urban test-beds are an effective means for developing, testing, and fostering the necessary basic and applied meteorological and socioeconomic research, and transitioning research findings to operations. An extended, multi-year period of continuous effort, punctuated with intensive observing and forecasting periods, is envisioned.

• Imaging Science in Dentistry
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• 제39권3호
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• pp.149-156
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• 2009

Purpose : The purpose of this study was to develop a stent-based image guided surgery system and to apply it to oral and maxillofacial surgeries for anatomically complex sites. Materials and Methods : We devised a patient-specific stent for patient-to-image registration and navigation. Three-dimensional positions of the reference probe and the tool probe were tracked by an optical camera system and the relative position of the handpiece drill tip to the reference probe was monitored continuously on the monitor of a PC. Using 8 landmarks for measuring accuracy, the spatial discrepancy between CT image coordinate and physical coordinate was calculated for testing the normality. Results : The accuracy over 8 anatomical landmarks showed an overall mean of $0.56{\pm}0.16\;mm$. The developed system was applied to a surgery for a vertical alveolar bone augmentation in right mandibular posterior area and possible interior alveolar nerve injury case of an impacted third molar. The developed system provided continuous monitoring of invisible anatomical structures during operation and 3D information for operation sites. The clinical challenge showed sufficient accuracy and availability of anatomically complex operation sites. Conclusion : The developed system showed sufficient accuracy and availability in oral and maxillofacial surgeries for anatomically complex sites.

• 한국수소및신에너지학회논문집
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• 제25권1호
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• pp.19-27
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• 2014

Mg hydride has a high hydrogen capacity (7.6%), at high temperature, and is a lightweight and low cost material, thus it a promising hydrogen storage material. However, its high operation temperature and very slow reaction kinetics are obstacles to practical application. In order to overcome these disadvantages of Mg hydride, graphene powder was added to it. The addition of graphene has been shown to reduce the operating temperature of dehydrogenation. Moreover, in this report the environmental aspects of $MgH_x$-Graphene composites are investigated by means of the environmental life cycle assessment (LCA) method. $MgH_x$-Graphene mixture was prepared by hydrogen induced mechanical alloy (HIMA). The synthesized powder was characterized by XRD(X-ray Diffraction). The hydrogenation behaviors were evaluated by using a Sievert's type automatic PCT apparatus. Such evaluation of Materials also conducted in the LCA. From the result of P-C-T(Pressure-Composition-Temperature) curves, the $MgH_x$-3wt.% graphene composite was evaluated as having a 5.86wt.% maximum hydrogen storage capacity, at 523K. From absorption kinetic testing, the $MgH_x$-7wt.% graphene composite was evaluated as having a maximum 6.94wt.%/ms hydrogen absorption rate, at 573K. Environment evaluation results for the $MgH_x$-graphene composites and other materials indicated environmental impact from the electric power used and from the materials themselves.

• Bulletin of the Korean Chemical Society
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• 제30권12호
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• pp.2999-3005
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• 2009

Among the more than 120 different types of human papillomavirus (HPV), types 16 and 18 have been known to be high risk agents that cause cervical cancer. We examined, in an immuno-chromatographic analysis, the potential of using the early gene product, E7 protein, as a diagnostic marker of cervical cancer caused by HPV. We developed monoclonal antibodies specific to HPV-16 and 18 E7 proteins that were produced from bacterial cells using gene recombinant technology. For each E7 protein, the optimal antibody pair was selected using the immuno-chromatographic sandwichtype binding system based on the lateral flow through membrane pores. Under these conditions, this rapid testing assay had a detection capability as low as 2 ng/mL of E7 protein. Furthermore, since viral analysis required the host cell to be lysed using chemicals such as detergents, it was possible that the E7 protein was structurally damaged during this process, which would result in a decrease in detection sensitivity. Therefore, we examined the detrimental effects caused by different detergents on the E7 protein using HeLa cells as the host. In these experiments, we found that the damage caused by the detergent, nonylphenylpolyethylene glycol (NP-40), was minimal relative to Triton X-100 commonly used for the cell lysis. Temperature also affected the stability of the E7 protein, and we found that the E7 protein was stabilized at 4$^{\circ}C$ for about 2 h, which was 4 times longer than at room temperature. Finally, a HPV-infected cervical cancer cell line, which was used as a real sample model, was treated using the optimized conditions and the presence of E7 proteins were analyzed by immuno-chromatography. The results of this experiment demonstrated that this rapid test could specifically detect HPV-infected samples.

• 한방안이비인후피부과학회지
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• 제33권3호
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• pp.27-44
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• 2020

Objectives : The authors conducted randomized, Double-blinded, and placebo-controlled parallel-group clinical experiment study to evaluate efficacy of fermented soybean(Bio-Peptone)cream in pruritus. Methods : The research had been conducted for 4 months from the date of IRB approval(May 26 in 2017) to Sept 2017. The experiment started by randomly distributing 25 subjects with pruritus into experimental group and control group, respectively. The experimental group applied fermented soybean(Bio-Peptone)cream twice a day, in the morning and evening, for one week on itchy area. The effect of the product was evaluated by comparing the PSS(Patient subjective score), moisture level by measuring skin moisture content(Corneometer) and transepidermal water loss(Tewameter), and the Korean version of Skindex-29(index of quality of life improvement) before applying the cream, after applying the cream for one week and after stop applying the cream for one week. The control group conducted identical experiment with the experimental group, except the control group applied placebo instead of the fermented soybean(Bio-Peptone)cream. Results : Pruritus, criterion of the first validation testing, indicates fermented soybean(Bio-Peptone)cream tend to reduce pruritus compare to placebo, although the result is not statistically noticeable. Significant difference in reduction of prutitus, the second validation test was not discovered in both groups. Corneometer and life quality tend to be improved with soy cream than placebo, but not statistically effective and both groups did not show any difference in terms of Tewameter measurement. Conclusions : The result of clinical experiment didn't prove that the fermented soybean(Bio-Peptone)cream is more effective in reducing pruritus than placebo, statistically. The clinical use of soybean product for pruritus requires further studies to be verified.

• Biomolecules & Therapeutics
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• 제14권4호
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• pp.253-258
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• 2006

LDD175(4-choloro-7-trifluoromethyl-10H-benzo[4,5]furo[3,2-b]indole-l-carboxylic acid) is one of benzofuroindole derivatives that act as a potent $BK_{Ca}$ channel openers. In the process of testing LDD175 as a new drug candidate, an acute toxicity study was carried out in mice. The mice were administered LDD175 intraperitoneally at dose of 0.2, 1, 10, 50, 100, 200, 400, 800mg/kg and orally at dose of 10, 100, 400, 800mg/kg body weight. After administering LDD175, the vital organs such as the liver, kidney and spleen were carefully observed for any significant pathological features or differences from the norm over a l4-day period. LDD175 did not induce any short-term toxicity at doses less than 100mg/kg. A $LD_{50}$ of LDD175 was 2493mg/kg in male mice and 4908mg/kg in female mice. Weight reduction was observed at a dose of 800mg/kg in male, and 400 and 800mg/kg in female. The kidney weight decreased in females after an intraperitoneal injection of LDD175 high dose(>400mg/kg, i.p.), and the spleen weight increased in the male(800mg/kg, i.p.) and female(400mg/kg, i.p.) mice. Inspite of the change in organ weights, there were neither histopathological changes nor any gross morphological abnormalities detected in any organ. LDD175 did not produce significant changes in the general behavior at doses of <200mg/kg, but decreased locomotor activity was observed at an intraperitoneal dose of 400mg/kg. Its effects on the locomotor activity and activity on the rotarod were tested and compared with the effects of diazepam 5mg/kg. The decrement in the locomotor activity and the activity on the rotarod induced by LDD175 was less serious than it by diazepam.

• 생명과학회지
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• 제15권6호
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• pp.968-971
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• 2005

본 연구는 Duroc, Landrace, Berkshire, Yorkshire를 기초 축으로 이용한 1003두에 대해 MC4R유전자의 PCR-RFLP를 이용하여 그 다형성을 조사하고 돼지의 일당증체량, 등지방 두께, 사료 요구율, 정육율과 그 유전자형 간의 연관성을 규명하고자 실시하였다. MC4R유전자에 대해 PCR-RFLP를 이용하여 226bp산물을 증폭한후 Taq I 체한효소로 사용하였다. 얻어진 MC4R gene의 유전자 빈도는 품종별로 다르게 나타났다. 통계적 분석을 통하여 각 유전자형에 대한 경제형질과 관련성을 분석한 결과 일당 증체량과 사료요구량은 NN 유전자형을 가진 개체들이 DN이나 DD유전자형을 가진 개체들에 비해 유의적으로 우수한 능력을 보였다(P < 0.05). 하지만 D 대립유전자는 높은 정육율과 낮은 등지방두께에 연관성이 있음을 관찰하였다. 따라서 돼지의 성장과 정육율과 관련된 선발력을 높이기 위해서 MC4R유전자의 다형성분석에서 검증된 PCR marker를 우량돼지육종 계획에 있어 분자생물학적 선발 marker로 사용할 수 있을 것으로 사료된다.

• 한국어병학회지
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• 제26권3호
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• pp.265-274
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• 2013

동해안의 3개 정점(삼척, 영덕, 포항)과 서해안의 2개 정점(학암포, 만리포)에서 여름철(7, 8월)에 진두발을 채집하여 생장, 성숙, 배우체와 사분포자체 비율 및 질병에 대한 연구를 실시하였다. 진두발의 체장은 개체군별로 평균 6.10~9.69 cm였으며 만리포에서 최소였고 포항에서 최대로서 동해안 개체가 서해안 개체에 비해 큰 것으로 확인되었다. 개체가 서해안 진두발에 비해 큰 것을 확인하였다. 진두발 개체군의 영양체 비율은 26.7~66.7 %였으며, 영양체를 resorcinol 방법으로 구분한 후 배우체:사분포자체 비율은 동해안에서 1.7:1로 배우체가 우점하였고 서해안에서는 학암포에서 1:1.1, 만리포에서 1:2.3로서 포자체가 번무하였다. 본 연구에서 한국산 진두발은 다양한 질병(백색증과 녹반증 등), 내생조류와 착생조류를 가지고 있었으며, 여름철의 진두발은 건강한 조체는 20~40 %로서 많은 개체가 질병을 가지고 있었다. 진두발은 현재까지 동정되지 않은 사상형 녹조류인 내생하는 Ulvella sp.를 가지고 있었다. 이러한 내생조류는 유럽과 캐나다의 주름진두발에서 관찰되는 감염성 병원체로서 보다 많은 관심과 연구가 요구된다.

• 식물분류학회지
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• 제46권4호
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• pp.361-370
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• 2016

한반도 남동부에 위치한 주왕산 국립공원과 그 인접산지는 식물 개체군의 유전자 이동에 대한 물리적 장벽의 영향을 시험하기 위한 훌륭한 모델 시스템이다. 우리는 식물종의 경우, 격리된 집단이 연속적인 분포를 보이는 집단보다 유전적인 분화 정도가 더 클 것으로 예측했다. 바위채송화 대부분의 집단은 4곳의 고립된 계곡에서 생육하며, 10개 집단에서 12 종류의 알로자임 유전좌위를 사용하여 유전적 다양성과 구조를 평가했다. 저자들은 이 연구와 기존 연구된 둥근잎꿩의비름(4곳 계곡에서 격리되어 생육)과 기린초(상대적으로 연속적으로 분포) 결과와 비교했다. 우리는 기린초 집단내 유전적 변이가 중간 수준임을 발견했다($H_e=0.112$). 바위채송화 집단간 분기 수준도 중간 수준($F_{ST}=0.250$)이었고 예상대로 둥근잎꿩의비름(0.261)과 유사했지만 기린초(0.165)보다 상당히 높았다. 분자분산분석(AMOVA) 결과 바위채송화와 둥근잎꿩의비름은 기린초(4%)보다 계곡간 변이(각각 19%) 비율이 높았다. STRUCTURE 프로그램 분석에 의하면 대부분의 이런 변이는 중간에 있는 두 계곡간의 유전적 조성 차이 때문이다. 저자들은 종간에 관찰된 분화 수준의 차이(즉, 기린초 대 바위채송화와 둥근잎꿩의비름)는 연구 지역 내의 그들의 분포 차이에 기인한다고 결론지었다.