• Animal Bioscience
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• v.35 no.9
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• pp.1303-1313
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• 2022

Objective: The current study aimed to perform whole-genome resequencing of Chinese indigenous Mongolian sheep breeds including Ujimqin, Sunit, and Wu Ranke sheep breeds (UJMQ, SNT, WRK) and deeply analyze genetic variation, population structure, domestication, and selection for domestication traits among these Mongolian sheep breeds. Methods: Blood samples were collected from a total of 60 individuals comprising 20 WRK, 20 UJMQ, and 20 SNT. For genome sequencing, about 1.5 ㎍ of genomic DNA was used for library construction with an insert size of about 350 bp. Pair-end sequencing were performed on Illumina NovaSeq platform, with the read length of 150 bp at each end. We then investigated the domestication and signatures of selection in these sheep breeds. Results: According to the population and demographic analyses, WRK and SNT populations were very similar, which were different from UJMQ populations. Genome wide association study identified 468 and 779 significant loci from SNT vs UJMQ, and UJMQ vs WRK, respectively. However, only 3 loci were identified from SNT vs WRK. Genomic comparison and selective sweep analysis among these sheep breeds suggested that genes associated with regulation of secretion, metabolic pathways including estrogen metabolism and amino acid metabolism, and neuron development have undergone strong selection during domestication. Conclusion: Our findings will facilitate the understanding of Chinese indigenous Mongolian sheep breeds domestication and selection for complex traits and provide a valuable genomic resource for future studies of sheep and other domestic animal breeding.

• Journal of the Korean Society for Library and Information Science
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• v.52 no.3
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• pp.193-214
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• 2018

This study aims to analyze enactment of China's old and rare books management. For the purpose, the review on China's legal system and management related to old and rare books were studied. And this study investigated 4 laws, 6 administrative regulations, 4 sector regulations, and 13 administrative normativity papers. Among those, 5 major sources for old and rare books management were identified as follows : 1) China's National Civilization Protection Law in 2013, 2) China's Old and Rare Book Organization Directive in 1989, 3) Regulation on Management of Research, Organization, and Publication of Old and Rare Books in 1986, 4) Standards on Grading Classification of Civilization Collections in 2001, and 5) Provisional Regulation on Civilization Auctioning in 2003. Based on the analysis of these sources, this study deduced implications in terms of Chinese old and rare books management such as the necessity to give considerations to 1) the importance of old and rare books management policy, 2) nationwide old and rare books management, 3) expansion of eduction and training of human resources, 4) establishment of institution specializing in old and rare books, and 5) special management of science of Chinese medicine.

• Journal of the Korean Society for Library and Information Science
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• v.38 no.4
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• pp.193-218
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• 2004

Electronic records created by Korean Government Agencies in the course of their business activities have been increased rapidly since e-Government had been implemented. With more government business being, conducted electronically, the importance of managing electronic records effectively, appropriately, and trustily has never been greater. For managing electronic records accountably and trustily, it to necessary to develop relevant and detail electronic records management regulation. For that reason, this study is to analyze Korean Acts and Foreign(USA, UK, Canada Australia) Acts, Regulations, or Policies related to electronic records management and to suggest legislative requirements which could guide to develop regulation or Act of electronic records management.

• Journal of Microbiology and Biotechnology
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• v.27 no.9
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• pp.1692-1700
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• 2017

Ralstonia solanacearum causes bacterial wilt in a wide variety of host plant species and produces a melanin-like blackish-brown pigment in stationary phase when grown in minimal medium supplemented with tyrosine. To study melanin production regulation in R. solanacearum, five mutants exhibiting overproduction of melanin-like pigments were selected from a transposon (Tn) insertion mutant library of R. solanacearum SL341. Most of the mutants, except one (SL341T), were not complemented by the original gene or overproduced melanins. SL341T showed Tn insertion in a gene containing a conserved domain of eukaryotic transcription factor. The gene was annotated as a hypothetical protein, given its weak similarity to any known proteins. Upon complementation with its original gene, the mutant strains reverted to their wild-type phenotype. SL341T produced 3-folds more melanin at 72 h post-incubation compared with wild-type SL341 when grown in minimal medium supplemented with tyrosine. The chemical analysis of SL341T cultural filtrate revealed the accumulation of a higher amount of homogentisate, a major precursor of pyomelanin, and a lower amount of dihydroxyphenylalanine, an intermediate of eumelanin, compared with SL341. The expression study showed a relatively higher expression of hppD (encoding hydroxyphenylpyruvate dioxygenase) and lower expression of hmgA (encoding homogentisate dioxygenase) and nagL (encoding maleylacetoacetate isomerase) in SL341T than in SL341. SL341 showed a significantly higher expression of tyrosinase gene compared with SL341T at 48 h post-incubation. These results indicated that R. solanacearum produced both pyomelanin and eumelanin, and the novel hypothetical protein is involved in the negative regulation of melanin production.

• Journal of the Korean BIBLIA Society for library and Information Science
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• v.33 no.3
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• pp.325-346
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• 2022

The purpose of this study was to measure the professional identity level of public librarians and to identify related factors. For this purpose, a questionnaire survey was conducted for librarians in public libraries in Seoul and Gyeonggi Province from October 28 to November 18, 2021 and 375 valid responses collected were analyzed. As a result of the study, among the factors of professional identity public librarians showed high level of professional identity in order of belief in self-regulation, sense of calling to the field, belief in public service, using the professional organization as a major referent, and autonomy. In addition, librarian's age, career, position and whether the director of the library was a librarian had a statistically significant difference in professional identity. This study has a significance in that the Hall Professionalism Inventory was used to measure participants' professional identity and it associates domestic public libraries' settings with professional identity.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.55 no.2
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• pp.151-158
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• 2010

Camelina sativa L., known as popular names "gold-of-pleasure" or "false flax" is an alternative oilseed crop that can be grown under different climatic and soil conditions. Up to date, however, the genomic information of Camelina has not been studied in detail. Therefore, a cDNA library was constructed and characterized from young leaves. The constructed cDNA library incorporated of 1334 cDNA clones and the size of the insertion fragments average was 736 base pair. We generated a total of 1269 high-quality expressed sequence tags (ESTs) sequences. The result of cluster analysis of EST sequences showed that the number of unigene was 851. According to subsequent analysis, the 476 (55.9%) unigenes were highly homologous to known function genes and the other 375 (44.1%) unigenes were unknown. Remaining 63 (7.4%) unigenes had no homology with any other peptide in NCBI database, indicating that these seemed to be novel genes expressed in leaves of Camelina. The database-matched ESTs were further classified into 17 categories according to their functional annotation. The most abundant of categories were "protein with binding function or cofactor requirement (27%)", "metabolism (11%)", "subcellular localization (11%)", "cellular transport, transport facilities and transport routes (7%)", "energy (6%)", "regulation of metabolism and protein function (6%)". Our result in this study provides an overview of mRNA expression profile and a basal genetic information of Camelina as an oilseed crop.

• Journal of Korean Library and Information Science Society
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• v.45 no.2
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• pp.93-116
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• 2014

This study analyzed the digital curation process targeting the foreign 10 university libraries for the efficient utilization of the research data. The analysis was conducted by dividing into 4 parts such as the selection and ingest of data, preservation, access and use, responsibilities and management. The results indicated that research data for selection and ingest include data produced by the faculty members or can be accessible in the university or research institution. The findings also showed that the institutions use Dublin Core, MODS, and PREMIS as a metadata for preservation. In the aspect of approach and use, regulation are decided based on the law and agreement. And in the aspect of responsibility and management, most elements are divided into the management, financial, technical, physical, and personal parts and each audit or sustainability is reviewed.

• Journal of Life Science
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• v.6 no.4
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• pp.286-292
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• 1996

Angiogenesis is a fundamental process by which new blood vessels are formed. It is essential in embryo development, and wound healing. Furthermore, malignant tumor growth and metastasis are also angiogenesis-dependent. In the catilage tissue, normal angiogenesis process is suppressed. In fact, it was reported that angiogenesis-inhibitory substances were isolated from the extracts of cow and shark catilage tissue. In order to isolate genes involved in the regulation of angiogenesis from a catilage fish, we constructed a shark cDNA library from Scylliohinus torazame. We then screened the library using hyman tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) gene as a probe. Among the 4 X 10$^{4}$ plaques screened, we isolated 2 positive clones (T-1, T-2). Restriction enzyme analysis revealed that the T-1 clone contains 0.8 kb cDNA insert, and the T-2 clone contains 1.2 kb and 2.2 kb inserts, respectively. Further DNA sequence analysis shows that the DNA sequence of the T-1 clone is 53% homologous to that of the human TIMP-1 gene.

• Journal of Korean Library and Information Science Society
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• v.23
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• pp.29-77
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• 1995

The purpose of this study is to investigate the effectiveness and value of reading, the role of reading for character building and reading education for the character building instruction. A great book can change a person's life and future. Especially, reading in the juvenile period is important because of their intellectual curiosity and sensitivity. The results of the study were as follows. (1) In order to solve the problem of the dehumanization, and the confusion of the sense of value among young adults, we should enrich and cultivate the character of young adults through the reading education. (2) The effects of reading for personality are verbal effects, experimental effects, cultural effects, reformational effects and recreational effects etc. (3) The value of reading in the past were inspiration, information and recreation. But, in recent times, the interest in therapeutic value of reading is increasing. The theraputic value of reading is identification, catharsis and insights of one's problem through the character in the works. (4) The role of reading for the character building are the assistance of self-realization, the promotion of self-regulation and the assistance of self-understanding. (5) Psychological needs causing reading activity are the desire to escape from reality, better socialization, recreation and pleasure. (6) The mechanism of character building through the reading are the enlargement of experience, better adjustment to the environment and the society. (7) Reading education for the solution of developmental tasks of the young adults is important. The standard reading lists of Japan Reading Society, the list compiled by Lee Kyung-Sik and the list of Park Yong Doo are the example of the selected reading list for the solution of developmental tasks.

• Genomics & Informatics
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• v.20 no.1
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• pp.2.1-2.11
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• 2022

The method of single-cell RNA sequencing has been rapidly developed, and numerous experiments have been conducted over the past decade. Their results allow us to recognize various subpopulations and rare cell states in tissues, tumors, and immune systems that are previously unidentified, and guide us to understand fundamental biological processes that determine cell identity based on single-cell gene expression profiles. However, it is still challenging to understand the principle of comprehensive gene regulation that determines the cell fate only with transcriptome, a consequential output of the gene expression program. To elucidate the mechanisms related to the origin and maintenance of comprehensive single-cell transcriptome, we require a corresponding single-cell epigenome, which is a differentiated information of each cell with an identical genome. This review deals with the current development of single-cell epigenomic library construction methods, including multi-omics tools with crucial factors and additional requirements in the future focusing on DNA methylation, chromatin accessibility, and histone post-translational modifications. The study of cellular differentiation and the disease occurrence at a single-cell level has taken the first step with single-cell transcriptome and is now taking the next step with single-cell epigenome.