Acknowledgement
We thank all the staff in the local breeding sheep farm for their participation. This work was supported by the Science and Technology Major Project of Inner Mongolia Autonomous Region of China (zdzx2018016; zdzx2018065), and Grassland talent innovative team of Inner Mongolia Autonomous Region of China (12000-12102621).
References
- Li M, Tian S, Jin L, et al. Genomic analyses identify distinct patterns of selection in domesticated pigs and Tibetan wild boars. Nat Genet 2013;45:1431-8. https://doi.org/10.1038/ng.2811
- Dutta P, Talenti A, Young R, et al. Whole genome analysis of water buffalo and global cattle breeds highlights convergent signatures of domestication. Nat Commun 2020;11:4739. https://doi.org/10.1038/s41467-020-18550-1
- Pan ZY, Li SD, Liu QY, et al. Whole-genome sequences of 89 Chinese sheep suggest role of RXFP2 in the development of unique horn phenotype as response to semi-feralization. Gigascience 2018;7: giy019. https://doi.org/10.1093/gigascience/giy019
- Jiang Y, Xie M, Chen WB, et al. The sheep genome illuminates biology of the rumen and lipid metabolism. Science 2014; 344:1168-73. https://doi.org/10.1126/science.1252806
- Zhao YX, Yang J, Lv FH, et al. Genomic reconstruction of the history of native sheep reveals the peopling patterns of nomads and the expansion of early pastoralism in East Asia. Mol Biol Evol 2017;34:2380-95. https://doi.org/10.1093/molbev/msx181
- Yang J, Li WR, Lv FH, et al. Whole-genome sequencing of native sheep provides insights into rapid adaptations to extreme environments. Mol Biol Evol 2016;33:2576-92. https://doi.org/10.1093/molbev/msw129
- Zhong T, Han JL, Guo J, et al. Tracing genetic differentiation of Chinese Mongolian sheep using microsatellites. Anim Genet 2011;42:563-5. https://doi.org/10.1111/j.1365-2052.2011.02181.x
- Liu ZH, Ji ZB, Wang GZ, Chao T, Hou L, Wang J. Genomewide analysis reveals signatures of selection for important traits in domestic sheep from different ecoregions. BMC Genomics 2016;17:863. https://doi.org/10.1186/s12864-016-3212-2
- Kang L, Han X, Zhang Z, Sun OJ. Grassland ecosystems in China: review of current knowledge and research advancement. Philos Trans R Soc Lond B Biol Sci 2007;362:997-1008. https://doi.org/10.1098/rstb.2007.2029
- Alberto FJ, Boyer F, Orozco-terWengel P, et al. Convergent genomic signatures of domestication in sheep and goats. Nat Commun 2018;9:813. https://doi.org/10.1038/s41467-018-03206-y
- Abied A, Xu L, Sahlu BW, et al. Genome-wide analysis revealed homozygosity and demographic history of five Chinese sheep breeds adapted to different environments. Genes (Basel) 2020;11:1480. https://doi.org/10.3390/genes11121480
- Abied A, Bagadi A, Bordbar F, et al. Genomic diversity, population structure, and signature of selection in five chinese native sheep breeds adapted to extreme environments. Genes (Basel) 2020;11:494. https://doi.org/10.3390/genes 11050494
- Zhang Y, Xue X, Liu Y, et al. Genome-wide comparative analyses reveal selection signatures underlying adaptation and production in Tibetan and Poll Dorset sheep. Sci Rep 2021;11:2466. https://doi.org/10.1038/s41598-021-81932-y
- Zhang T, Gao H, Sahana G, et al. Genome-wide association studies revealed candidate genes for tail fat deposition and body size in the Hulun Buir sheep. J Anim Breed Genet 2019; 136:362-70. https://doi.org/10.1111/jbg.12402
- Zhi D, Da L, Liu M, et al. Whole genome sequencing of hulunbuir short-tailed sheep for identifying candidate genes related to the short-tail phenotype. G3 (Bethesda) 2018;8: 377-83. https://doi.org/10.1534/g3.117.300307
- Pan Z, Li S, Liu Q, et al. Rapid evolution of a retro-transposable hotspot of ovine genome underlies the alteration of BMP2 expression and development of fat tails. BMC Genomics 2019;20:261. https://doi.org/10.1186/s12864-019-5620-6
- Li X, Yang J, Shen M, et al. Whole-genome resequencing of wild and domestic sheep identifies genes associated with morphological and agronomic traits. Nat Commun 2020; 11:2815. https://doi.org/10.1038/s41467-020-16485-1
- Bolger AM, Lohse M, Usadel B. Trimmomatic: a flexible trimmer for Illumina sequence data. Bioinformatics 2014; 30:2114-20. https://doi.org/10.1093/bioinformatics/btu170
- Li H, Durbin R. Fast and accurate long-read alignment with Burrows-Wheeler transform. Bioinformatics 2010;26:589-95. https://doi.org/10.1093/bioinformatics/btp698
- McKenna A, Hanna M, Banks E, et al. The genome analysis toolkit: a mapreduce framework for analyzing next-generation DNA sequencing data. Genome Res 2010;20:1297-303. https://doi.org/10.1101/gr.107524.110
- McLaren W, Gil L, Hunt SE, et al. The ensembl variant effect predictor. Genome Biol 2016;17:122. https://doi.org/10.1186/s13059-016-0974-4
- Yin L, Zhang H, Tang Z, et al. rMVP: A memory-efficient, visualization-enhanced, and Parallel-accelerated tool for genome-wide association study. Genomics Proteomics Bioinformatics 2021;19:619-28. https://doi.org/10.1016/j.gpb.2020.10.007
- Alexander DH, Novembre J, Lange K. Fast model-based estimation of ancestry in unrelated individuals. Genome Res 2009;19:1655-64. https://doi.org/10.1101/gr.094052.109
- Li H, Durbin R. Inference of human population history from individual whole-genome sequences. Nature 2011;475:493-6. https://doi.org/10.1038/nature10231
- Weir BS, Cockerham CC. Estimating F-statistics for the analysis of population structure. Evolution 1984;38:1358-70. https://doi.org/10.1111/j.1558-5646.1984.tb05657.x
- Tajima F. Statistical method for testing the neutral mutation hypothesis by DNA polymorphism. Genetics 1989;123:585-95. https://doi.org/10.1093/genetics/123.3.585
- Beissbarth T, Speed TP. GOstat: find statistically overrepresented Gene Ontologies within a group of genes. Bioinformatics 2004;20:1464-5. https://doi.org/10.1093/bioinformatics/bth088
- Wei C, Wang H, Liu G, et al. Genome-wide analysis reveals population structure and selection in Chinese indigenous sheep breeds. BMC Genomics 2015;16:194. https://doi.org/10.1186/s12864-015-1384-9
- Ganbold O, Lee S-H, Seo D, et al. Genetic diversity and the origin of Mongolian native sheep. Livest Sci 2019;220:17-25. https://doi.org/10.1016/j.livsci.2018.12.007
- Ehlers J, Gibbard PL. The extent and chronology of cenozoic global glaciation. Quat Int 2007;164-65:6-20. https://doi.org/10.1016/j.quaint.2006.10.008
- Lehmkuhl F, Owen LA. Late quaternary glaciation of Tibet and the bordering mountains: a review. Boreas 2005;34:87-100. https://doi.org/10.1111/j.1502-3885.2005.tb01008.x
- Kim J, Hu Z, Cai L, et al. CPS1 maintains pyrimidine pools and DNA synthesis in KRAS/LKB1-mutant lung cancer cells. Nature 2017;546:168-72. https://doi.org/10.1038/nature22359
- Talebi M, Mehrjardi MYV, Kalhor K, Dehghani M. Is there any relationship between mutation in CPS1 Gene and pregnancy loss? Int J Reprod Biomed 2019;17:371-4. https://doi.org/10.18502/ijrm.v17i5.4604
- Matsumoto M, Miki T, Shibasaki T, et al. Noc2 is essential in normal regulation of exocytosis in endocrine and exocrine cells. Proc Natl Acad Sci USA 2004;101:8313-8. https://doi.org/10.1073/pnas.0306709101
- Ganbold O, Manjula P, Lee SH, et al. Sequence characterization and polymorphism of melanocortin 1 receptor gene in some goat breeds with different coat color of Mongolia. Asian-Australas J Anim Sci 2019;32:939-48. https://doi.org/10.5713/ajas.18.0819
- Tong SQ, Bao YH, Te RL, Ma Q, Ha S, Lusi A. Analysis of drought characteristics in Xilingol Grassland of Northern China based on SPEI and its impact on vegetation. Math Probl Eng 2017;2017:5209173. https://doi.org/10.1155/2017/5209173