• Journal of Radiation Industry
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• v.4 no.1
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• pp.65-71
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• 2010

Two chitinase producing strains CHI2 and CHI4 were isolated from soybean rhizosphere soil. Both the strains belonged to Lysobacter enzymogenes as indicated by 16S rDNA sequence analysis. Though strain CHI2 and CHI4 produced extracellular chitinase, they differ in their chitinolytic activity. CHI4 produced approximately three times the higher amounts of enzyme than that of CHI2 under specified conditions. CHI2 produced $535.67U\;l^{-1}$ of chitinase after 48 h incubation with a specific activity of $3.91U\;mg^{-1}$ of protein while strain CHI4 produced $1584.13U\;l^{-1}$ of chitinase with a specific activity of $10.88U\;mg^{-1}$ protein. SDS-PAGE analysis indicated that the molecular weight of chitinase enzyme was approximately 45 kDa. A faint band with a molecular weight of 55 kDa reveals the possibility for the presence of another kind of chitin binding protein. Mutant library was developed by exposing the isolates to gamma rays at their $LD_{99}$ value (0.23 kGy). Totally, 11 mutants of CHI2 and CHI4 are reported to have enhanced chitinase activity. Several leaky mutant clones with decreased enzyme activity and a defective mutant (CHI2-M16) with complete loss of chitinase activity were also identified. CHI4-M18, CHI4-M8 and CHI4-M29 showed 78.8, 41.5, and 31.9% increased chitinase activity over wild type CHI4.

• Biomedical Science Letters
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• v.6 no.1
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• pp.1-9
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• 2000

To identity the genes responsible for the biosynthesis of exopolysaccharide, recombinant plasmids pUEX10 and pLEX10 were constructed from plasmid pLEX3 which was isolated from the recombinant cosmid library of Zoogloea ramigera 115. The complete nucleotide sequence of the 1.7 kb genomic DNA insert in plasmid pUEX10 was determined. Its analysis identified two open reading frames (ORF3 & ORF4) which could encode two proteins. The amino acid sequence derived from ORF3 showed the homology with gumC protein in Xanthomonas campestris as well as exoP protein in Rhizobium melizoti. The partial amino acid sequence of ORF4 showed the homology with polysaccharide export protein in Thermotoga maritima. Z. ramigera 115SLR and Z. ramigera 115SLR/pLEX10 showed the similar pattern for EPS production. Yield of exopolysaccharides produced by Z. ramigera 115SLR and Z. ramigera 115SLR/pLEX10 was 0.26% (w/v) and 0.16% (w/v), respectively.

• Journal of the Korean Society for Library and Information Science
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• v.46 no.4
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• pp.169-188
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• 2012

This study aimed to analyze the characteristics of the bibliographic description of ISBD Consolidated edition published in 2011 and to grasp the considerations in applying the rules of ISBD consolidated edition to KCR4. For achieving this, the four aspects were analyzed such as the description area and data elements, the resource types, the punctuation, and the order of the elements of ISBD Consolidated edition(2011). The characteristics of ISBD Consolidated edition are as follows. First, the content form and the media type area are added in new 0 area and elements are designated by mandatory to confirm to FRBR. Second, content form, content qualification and media type replaced GMD in title and statement of responsibility area. Third, the prescribed punctuations were retained even when this results in double punctuation, and individual square brackets were preferred than entire square brackets when using square brackets to all elements in same area. Fourth, the order of elements in description was set out by patterns of data elements in areas, therefore could reduce the confusion of the order of elements. ISBD Consolidated edition as an international standard would make various rules to maintain the uniformity, but also respects the bibliographic practices of individual countries. Therefore, each country must revise its own rule to conform the ISBD Consolidated edition as well as reflect its unique situation. In Korea, since KCR4 was developed based on the previous edition of ISBD, it should be revised to confirm to the ISBD Consolidated edition. Therefore, this study is expected to contribute to the revision of KCR4.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.48 no.1
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• pp.22-30
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• 2011

In this paper, we propose a memory efficient multi-rate Low Density Parity Check (LDPC) decoder for China Mobile Multimedia Broadcasting (CMMB). We find the best trade-off between the performance and the circuit area by designing a partially parallel decoder which is capable of passing multiple messages in parallel. By designing an efficient address generation unit (AGU) with an index matrix, we could reduce both the amount of memory requirement and the complexity of computation. The proposed regular LDPC decoder was designed in Verilog HDL and was synthesized by Synopsys' Design Compiler using Chartered $0.18{\mu}m$ CMOS cell library. The synthesized design has the gate size of 455K (in NAND2). For the two code rates supported by CMMB, the rate-1/2 decoder has a throughput of 14.32 Mbps, and the rate-3/4 decoder has a throughput of 26.97 Mbps. Compared with a conventional LDPC for CMMB, our proposed design requires only 0.39% of the memory.

• JSTS:Journal of Semiconductor Technology and Science
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• v.7 no.4
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• pp.247-253
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• 2007

In this paper, a high throughput multiple transform architecture for H.264 Fidelity Range Extensions (FRExt) is proposed. New techniques are adopted which (1) regularize the $8{\times}8$ integer forward and inverse DCT transform matrices, (2) divide them into four $4{\times}4$ sub-matrices so that simple fast butterfly algorithm can be used, (3) because of the similarity of the sub-matrices, mixed butterflies are proposed that all the sub-matrices of $8{\times}8$ and matrices of $4{\times}4$ forward DCT (FDCT), inverse DCT (IDCT) and Hadamard transform can be merged together. Based on these techniques, a hardware architecture is realized which can achieve throughput of 1.488Gpixel/s when processing either $4{\times}4\;or\;8{\times}8$ transform. With such high throughput, the design can satisfy the critical requirement of the real-time multi-transform processing of High Definition (HD) applications such as High Definition DVD (HD-DVD) ($1920{\times}1080@60Hz$) in H.264/AVC FRExt. This work has been synthesized using Rohm 0.18um library. The design can work on a frequency of 93MHz and throughput of 1.488Gpixel/s with a cost of 56440 gates.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.27 no.9C
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• pp.861-870
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• 2002

In this paper, with targeting on the drawback of RSA of operation speed, a new 1024-bit RSA cryptosystem has been proposed and implemented in hardware to increase the operational speed and perform the variable-length encryption. The proposed cryptosystem mainly consists of the modular exponentiation part and the modular multiplication part. For the modular exponentiation, the RL-binary method, which performs squaring and modular multiplying in parallel, was improved, and then applied. And 4-stage CSA structure and radix-4 booth algorithm were applied to enhance the variable-length operation and reduce the number of partial product in modular multiplication arithmetic. The proposed RSA cryptosystem which can calculate at most 1024 bits at a tittle was mapped into the integrated circuit using the Hynix Phantom Cell Library for Hynix 0.35㎛ 2-Poly 4-Metal CMOS process. Also, the result of software implementation, which had been programmed prior to the hardware research, has been used to verify the operation of the hardware system. The size of the result from the hardware implementation was about 190k gate count and the operational clock frequency was 150㎒. By considering a variable-length of modulus number, the baud rate of the proposed scheme is one and half times faster than the previous works. Therefore, the proposed high speed variable-length RSA cryptosystem should be able to be used in various information security system which requires high speed operation.

• Journal of Microbiology and Biotechnology
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• v.24 no.12
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• pp.1699-1706
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• 2014

A lichenase gene (mt-lic) was identified for the first time through function-based screening of a soil metagenomic library. Its deduced amino acid sequence exhibited a high degree of homology with endo-${\beta}$-1,3-1,4-glucanase (having both lichenase and chitosanase activities), encoded by the bgc gene of Bacillus circulans WL-12. The recombinant lichenase overexpressed and purified from Escherichia coli was able to efficiently hydrolyze both barley ${\beta}$-glucan and lichenan. The enzyme showed maximal activity at a pH of 6.0 at $50^{\circ}C$, with Azo-barley-glucan as the substrate. The metal ions $Mn^{2+}$, $Mg^{2+}$, $Ca^{2+}$, and $Fe^{2+}$ enhanced the enzymatic activity, whereas the $Cu^{2+}$ and $Zn^{2+}$ ions inhibited the enzymatic activity. The $K_m$ and $V_{max}$ values of the purified lichenase were determined to be 0.45 mg/ml and 24.83 U/min/mg of protein, respectively.

• Korean Journal of Clinical Pharmacy
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• v.29 no.4
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• pp.247-253
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• 2019

Background: Glutamate is implicated in the pathophysiology of migraine, a common neurological disorder. Therefore, glutamate receptor antagonists (GluRAs) have been suggested as a novel migraine treatment that are able to overcome the limitations of triptans. Objective: The aim of this study was to perform a meta-analysis to assess the efficacy of GluRAs for patients with migraine. Method: The PubMed, Cochrane Library, CINAHL, and Clinical Trial.gov databases were searched for randomized placebo-controlled trials of the efficacy of GluRAs for patients with migraine conducted up to August 2019. Two independent reviewers screened the literature according to inclusion and exclusion criteria and performed quality assessment and data extraction. Review Manager 5.3 software was used for the meta-analysis. Results: Three studies involving a total of 206 patients were included in the final analysis. Compared with placebo, GluRAs significantly improved the pain-free response at 2 hours (odds ratio [OR]=3.85, 95% confidence intervals [CIs]=1.63-9.09) and the 24-hour sustained pain freedom (OR=7.40; 95% CIs=2.36-23.20). The use of rescue medications with GluRAs was lower compared to that with placebo, but the difference was not significant (OR=0.39, 95% CI=0.10-1.47). Conclusion: Our meta-analysis showed that GluRAs were more effective than placebo for patients with migraine.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.23 no.8
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• pp.2120-2130
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• 1998

This paper concerns a design of programmable MPEG decoder for video processing by object unit on network. The decoder can process video data effectively by a embedded controller with stack buffers for supporting OOP (Object-Oriented Programming). The controller offers extended instructions that process several data types including 32bit integer type. In addition to that, we have a vector processor, in this decoder that can execute advanced compensation and prediction by half pixel and SA(Shape Adaptive)-IDCT of MPEG-4. Absolutors and halfers in the vector processor make this architecture extensive to a encoder. We verified the decoder with $0.6\mu\textrm{m}$ 5-Volt CMOS COMPASS library.

• Journal of the Institute of Electronics Engineers of Korea CI
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• v.38 no.6
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• pp.82-92
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• 2001

This paper describes implementation of an embedded 32-Bit RISC core for portable communication/information equipment, such as cellular phones, PDA(Personal Digital Assistants), notebook, etc. The RISC core implements the ARM$\circled$V 4 instruction set, operates with typical 5-stage pipeline. It supports Thumb code to improve the code density, and uses the dynamic power management method of pipeline registers. It was modeled and simulated in RTL level using VHDL, and verified with ARMulator of ADS (Arm Developer Suite) and had average CPI of 1.44. The core is synthesized automatically using the cell library based on $0.6{\mu}m$ CMOS 1-poly 3-metal CMOS technology. It consists of about 41,000 gates and the clock frequency is expected to be above 45 MHz.