• Animal Bioscience
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• v.36 no.1
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• pp.10-18
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• 2023

Objective: In this study, we aimed to position the Hungarian Merino among other Merinoderived sheep breeds, explore the characteristics of our sampled animals' genetic similarity network within the breed, and highlight single nucleotide polymorphisms (SNPs) associated with daily weight-gain. Methods: Hungarian Merino (n = 138) was genotyped on Ovine SNP50 Bead Chip (Illumina, San Diego, CA, USA) and positioned among 30 Merino and Merino-derived breeds (n = 555). Population characteristics were obtained via PLINK, SVS, Admixture, and Treemix software, within-breed network was analysed with python networkx 2.3 library. Daily weight gain of Hungarian Merino was standardised to 60 days and was collected from the database of the Association of Hungarian Sheep and Goat Breeders. For the identification of loci associated with daily weight gain, a multi-locus mixed-model was used. Results: Supporting the breed's written history, the closest breeds to Hungarian Merino were Estremadura and Rambouillet (pairwise F_ST values are 0.035 and 0.036, respectively). Among Hungarian Merino, a highly centralised connectedness has been revealed by network analysis of pairwise values of identity-by-state, where the animal in the central node had a betweenness centrality value equal to 0.936. Probing of daily weight gain against the SNP data of Hungarian Merinos revealed five associated loci. Two of them, OAR8_17854216.1 and s42441.1 on chromosome 8 and 9 (-log₁₀P>22, false discovery rate<5.5e-20) and one locus on chromosome 20, s28948.1 (-log₁₀P = 13.46, false discovery rate = 4.1e-11), were close to the markers reported in other breeds concerning daily weight gain, six-month weight, and post-weaning gain. Conclusion: The position of Hungarian Merino among other Merino breeds has been determined. We have described the similarity network of the individuals to be applied in breeding practices and highlighted several markers useful for elevating the daily weight gain of Hungarian Merino.

• Proceedings of the KSAR Conference
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• 2001.03a
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• pp.58-58
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• 2001

본 연구는 섬유소 분해효소 유전자 Cel D(Cellulase Digestion)가 도입된 형질전환 돼지 생산을 통하여 섬유소 함유 사료의 이용 효율을 증대시키고, 나아가 장기이식동물 및 고가의 의료용 단백질 생산가축을 개발하기 위한 원천기술 확보에 있다. 섬유소분해 유전자의 크로닝 및 조직 특이적 발현벡터를 개발하기 위하여, 우선 소의 위중 제4위 내의 미생물로부터 전체 유전자를 분리하였고, 이렇게 작성된 DNA library에서 섬유소분해 관련 유전자인 약 2.0 kb의 Cel D유전자를 크로닝하였으며, 췌장 특이적 발현 프로모터(rat elastase I: 약 200bp)를 크로닝한 후, 미세주입용 형질전환 재조합 벡터를 구축하기 위하여 rElastase I 프로모터 하류에 섬유소 분해 유전자(Cel D)를 연결하여 약 3.0 kb 크기의 재조합 벡터를 준비하였으며, 재조합 유전자를 1세포기 수정란 전핵내에 미세주입 하기 위해 Sal I과 BglII를 이용 유전자 단편을 만들었다. 구축된 유전자를 미세주입하기 위한 수정란을 회수하기위해 총68두의 돼지를 4-5두씩 분리사육하면서 발정동기화 및 과배란 유기를 위해 PG600, Altrenogest, FSH, hCG를 투여하였으며 hCG투여후 약54시간에 외과적 방법에 의해 총 1,359개의 수정란을 회수하였고, 이중 미세주입가능한 1세포기 수정란은 1,296개로 두당 평균 15.9개 였다. 1,296개의 1세포기 수정란 중에서 재조합 유전자(rE I-CelD)가 미세주입된 660개의 수정란을 32두의 수란돈에 외과적 방법에 의해 이식하였으며, 이식되어진 모돈 13두가 분만하여 40.6%의 임신율을 나타내었다. 이렇게 분만된 13두에서 총 65두(암:33두, 수:32두)의 자돈이 생산되었으며, 형질전환 여부를 판명하기 위해 자돈의 꼬리조직으로 부터 genomic DNA를 추출하고 PCR 검정을 실시하였다. PCR 검정 결과, 섬유소 분해 유전자가 도입된 자돈은 5두 이었으며, 그 결과를 Table에 나타내었다.(Table Omitted) Table 1 에서와 같이 섬유소 분해효소유전자가 형질전환된 자돈은 65마리 중 5마리로 7.69%의 형질전환율을 나타내었으며, 5마리의 자돈중 2두(암:1두, 수:1두)는 분만 후 즉시 폐사되었으며 2두(암:1두, 수:1두)는 86일령 그리고 14일령에 폐사하여 현재 1두(암)가 생존하여 섬유소 분해 사양 실험 중에 있다.

• The Bulletin of The Korean Astronomical Society
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• v.35 no.1
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• pp.76.1-76.1
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• 2010

With advancement of infrared space telescopes during the past decade, infrared wavelength regime has been a focal point to study various properties of galaxies, such as stellar mass, dust contents and dust-hidden star formation with respect to evolution of galaxies. Polycyclic Aromatic Hydrocarbons (PAHs) have emerged as one of the most important features since these features dominate mid-infrared spectra of galaxies. These PAH features provide a great handle to calibrate star formation rates and diagnose ionized states of grains. However, PAH $3.3{\mu}m$ feature has not been studied as much as other PAH features since it is weaker than others and resides outside of Spitzer's capability. Still its calibration and characterization are important since it will be the only PAH feature accessible by JWST for high-z galaxies. AKARI mJy Unbiased Survey of Extragalactic Sources in 5MUSES (AMUSES) intends to take advantage of AKARI's capability of spectroscopy on 2 to 5 ${\mu}m$ to provide an unbiased library of 44 sample galaxies selected from a parent sample of 5MUSES, one of Spitzer legacy projects. For these 3.3mm flux limited sample galaxies whose redshifts range between 0 < z <1, AMUSES will calibrate PAH $3.3{\mu}m$ as a SFR while measuring ratios between PAH features and investigating Bra's potential as a SFR indicator. We present preliminary results of AMUSES.

• BMB Reports
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• v.34 no.4
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• pp.316-321
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• 2001

Dehydroascorbate (DHA) reductase (DHAR, EC 1.8.5.1) catalyzes the reduction of DHA to reduced ascorbate (AsA) using glutathione (GSH) as the electron donor in order to maintain an appropriate level of ascorbate in plant cells. To analyze the physiological role of DHAR in environmental stress adaptation, we developed transgenic tobacco (Nicotiana tabacum cv. Xanthi) plants that express a human DHAR gene isolated from the human fetal liver cDNA library in the chloroplasts. We also investigated the DHAR activity, levels of ascorbate, and GSH. Two transgenic plants were successfully developed by Agrobacterium-mediated transformation and were confirmed by PCR and Southern blot analysis. DHAR activity and AsA content in mature leaves of transgenic plants were approximately 1.41 and 1.95 times higher than in the non-transgenic (NT) plants, respectively In addition, the content of oxidized glutathione (GSSG) in transgenic plants was approximately 2.95 times higher than in the NT plants. The ratios of AsA to DHA and GSSG to GSH were changed by overexpression of DHAR, as expected, even though the total content of ascorbate and glutathione was not significantly changed. When tobacco leaf discs were subjected to methyl viologen at $5\;{\mu}M$, $T_0$ transgenic plants showed about a 50% reduction in membrane damage compared to the NT plants.

• Journal of Microbiology and Biotechnology
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• v.21 no.7
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• pp.667-674
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• 2011

The gene for esterase (rEst1) was isolated from a new species of genus Rheinheimera by functional screening of E. coli cells transformed with the pSMART/HaeIII genomic library. E. coli cells harboring the esterase gene insert could grow and produce clear halo zones on tributyrin agar. The rEst1 ORF consisted of 1,029 bp, corresponding to 342 amino acid residues with a molecular mass of 37 kDa. The signal P program 3.0 revealed the presence of a signal peptide of 25 amino acids. Esterase activity, however, was associated with a homotrimeric form of molecular mass 95 kDa and not with the monomeric form. The deduced amino acid sequence showed only 54% sequence identity with the closest lipase from Cellvibrio japonicus strain Ueda 107. Conserved domain search and multiple sequence alignment revealed the presence of an esterase/ lipase conserved domain consisting of a GXSXG motif, HGGG motif (oxyanion hole) and HGF motif, typical of the class IV hormone sensitive lipase family. On the basis of the sequence comparison with known esterases/ lipases, REst1 represents a new esterase belonging to the class IV family. The purified enzyme worked optimally at $50^{\circ}C$ and pH 8, utilized pNP esters of short chain lengths, and showed best catalytic activity with p-nitrophenyl butyrate ($C_4$), indicating that it was an esterase. The enzyme was completely inhibited by PMSF and DEPC and showed moderate organotolerance.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.17 no.1
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• pp.11-20
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• 2007

A fast Montgomery multiplication occupies important to the design of RSA cryptosystem. Montgomery multiplication consists of two addition, which calculates using CSA or RBA. In terms of CSA, the multiplier is implemented using 4-2 CSA o. 5-2 CSA. In terms of RBA, the multiplier is designed based on redundant binary system. In [1], A new redundant binary adder that performs the addition between two binary signed-digit numbers and apply to Montgomery multiplier was proposed. In this paper, we reconstruct the logic structure of the RBA in [1] for reducing time and space complexity. Especially, the proposed RB multiplier has no coupler like the RBA in [1]. And the proposed RB multiplier is suited to binary exponentiation as modified input and output forms. We simulate to the proposed NRBA using gates provided from SAMSUNG STD130 $0.18{\mu}m$ 1.8V CMOS Standard Cell Library. The result is smaller by 18.5%, 6.3% and faster by 25.24%, 14% than 4-2 CSA, existing RBA, respectively. And Especially, the result is smaller by 44.3% and faster by 2.8% than the RBA in [1].

• Journal of Microbiology
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• v.45 no.2
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• pp.105-112
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• 2007

The objective of this study was to analyze the phylogenetic composition of bacterial community in the soil of an earth-cave (Niu Cave) using a culture-independent molecular approach. 16S rRNA genes were amplified directly from soil DNA with universally conserved and Bacteria-specific rRNA gene primers and cloned. The clone library was screened by restriction fragment length polymorphism (RFLP), and representative rRNA gene sequences were determined. A total of 115 bacterial sequence types were found in 190 analyzed clones. Phylogenetic sequence analyses revealed novel 16S rRNA gene sequence types and a high diversity of putative bacterial community. Members of these bacteria included Proteobacteria (42.6%), Acidobacteria (18.6%), Planctomycetes (9.0 %), Chloroflexi (Green nonsulfur bacteria, 7.5%), Bacteroidetes (2.1%), Gemmatimonadetes (2.7%), Nitrospirae (8.0%), Actinobacteria (High G+C Gram-positive bacteria, 6.4%) and candidate divisions (including the OP3, GN08, and SBR1093, 3.2%). Thirty-five clones were affiliated with bacteria that were related to nitrogen, sulfur, iron or manganese cycles. The comparison of the present data with the data obtained previously from caves based on 16S rRNA gene analysis revealed similarities in the bacterial community components, especially in the high abundance of Proteobacteria and Acidobacteria. Furthermore, this study provided the novel evidence for presence of Gemmatimonadetes, Nitrosomonadales, Oceanospirillales, and Rubrobacterales in a karstic hypogean environment.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.39 no.12
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• pp.513-519
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• 2002

This paper proposes a high-speed FFT processor for orthogonal frequency-division multiplexing(OFDM) systems. The Proposed architecture uses a single-memory architecture and uses a radix-4 algorithm for high speed. The proposed memory is partitioned into four banks for high-speed computation. It uses an in-place memory strategy that stores butterfly outputs in the same memory location used by butterfly inputs. Therefore, the memory size can be reduced. The SQNR of about 80dB is achieved with 20-bit input and 20-bit twiddle factors. The architecture has been modeled by VHDL and logic synthesis has been performed using the SamsungTM 0.5㎛ SOG cell library (KG80). The implemented FFT processor consists of 98,326 gates excluding memory. It has smaller hardware than existing pipeline FFT processors for more than 1024-point FFTs. The processor can operate at 42MHz and calculate a 256-point complex FFT in 6us. It satisfies tile required processing speed of 8.4㎲ in the HomePlug standard.

• Journal of Environmental Science International
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• v.27 no.8
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• pp.723-735
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• 2018

In this study, to investigate an optimal configuration method for the modeling system, we performed an optimization experiment by controlling the types of compilers and libraries, and the number of CPU cores because it was important to provide reliable model data very quickly for the national air quality forecast. We were made up the optimization experiment of twelve according to compilers (PGI and Intel), MPIs (mvapich-2.0, mvapich-2.2, and mpich-3.2) and NetCDF (NetCDF-3.6.3 and NetCDF-4.1.3) and performed wall clock time measurement for the WRF and CMAQ models based on the built computing resources. In the result of the experiment according to the compiler and library type, the performance of the WRF (30 min 30 s) and CMAQ (47 min 22 s) was best when the combination of Intel complier, mavapich-2.0, and NetCDF-3.6.3 was applied. Additionally, in a result of optimization by the number of CPU cores, the WRF model was best performed with 140 cores (five calculation servers), and the CMAQ model with 120 cores (five calculation servers). While the WRF model demonstrated obvious differences depending on the number of CPU cores rather than the types of compilers and libraries, CMAQ model demonstrated the biggest differences on the combination of compilers and libraries.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.16 no.4
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• pp.765-770
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• 2012

In this paper, we proposed an effective hardware structure using DCT-based inra-prediction mode selection to reduce computational complexity caused by intra mode decision. In this hardware structure, the input block is transformed at first and then analyzed to determine its texture directional tendency. the complexity has solved by performing intra prediction in only predicted edge direction. $4{\times}4$ DCT is calculated in one cycle using Multitransform_PE and Inta_pred_PE calculates one prediction mode in two cycles. Experimental results show that the proposed Intra prediction encoding needs only 517 cycles for one macroblock encoding. This architecture improves the performance by about 17% than previous designs. For hardware implementation, the proposed intra prediction encoder is implemented using Verilog HDL and synthesized with Megnachip $0.18{\mu}m$ standard cell library. The synthesis results show that the proposed architecture can run at 125MHz.