• 한국식생활문화학회지
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• 제25권3호
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• pp.350-356
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• 2010

DPPH 라디칼 소거 활성 측정 결과 발효장뇌삼엽차의 추출물이 장뇌삼엽차의 추출물보다 활성이 더 높은 것으로 나타났다. 또한 장뇌삼과 발효장뇌삼이 장뇌삼엽차와 발효장 뇌삼엽차보다 항산화 활성이 높은 결과를 얻었다. 장뇌삼과 발효장뇌삼의 항산화 활성은 비슷하였다. SOD 유사활성도 측정에서는 대조군인 vitamin C와 비교할 때 모든 군이 SOD 유사활성도가 약한 것으로 분석되었다. 아질산염 소거능은 pH 1.2, 3.0의 조건에서 1 mg/mL의 농도로 처리 하였을 때 아질산염 소거능이 장뇌삼엽차, 발효장뇌삼, 장뇌삼, 발효장뇌삼의 순으로 높은 것으로 나타났고, pH 6.0의 조건에서는 모든 군이 아질산염 소거능이 약한 것으로 분석되었다. HeLa cell에서 시료 중 장뇌삼과 발효장뇌삼엽차의 추출물이 1 mg/mL의 농도에서 30% 이상의 암세포 증식 억제율을 나타내었다. 발효장뇌삼과 장뇌삼엽차는 각각 약 24%, 22%로 분석되었다. MCF-7 cell 발효장뇌삼과 장뇌삼엽차의 추출물이 1 mg/mL의 농도에서 27% 이상의 암세포 증식 억제율을 나타내어 비슷하였고, 장뇌삼 추출액이 21%로 가장 낮았으며, 발효장뇌삼엽차 추출액이 70% 이상으로 나타나 암세포 증식 억제율이 각 시료 중 가장 높은 것으로 분석되었다.

• 한국식품조리과학회지
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• 제27권6호
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• pp.763-772
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• 2011

This study evaluated the antioxidative activity and quality characteristics of Sulgidduk (traditional rice cakes) prepared with ramie leaf powder, in order to measure the antioxidative activity of ramie leaves, which are a potentially useful antioxidant food. Antioxidant activities were measured by total phenolic contents, the scavenging activities of DPPH radicals and ABTS+ radicals, and the reducing power. The total phenolic content of freeze dried ramie leaves was estimated at $58.70{\pm}11.42mg$ GAE/g. The $IC_{50}$ values for DPPH radicals and the ABTS+ radical scavenging activity of the ramie leaves were $23.76{\pm}1.41ug/mL$ and $174.82{\pm}6.4ug/mL$, respectively. The leaves also showed a high reducing power of $1.1057{\pm}0.03$ (O.D). To increase the utilizability of ramie leaf, we made Sulgidduk with added ramie leaf powder. Re-grading the quality characteristics of the altered Sulgidduk, the 3% and 5% addition levels most satisfied texture profile analysis and sensory evaluation. The antioxidative effect of the Sulgidduk significantly increased as the addition level increased, compared to the original Sulgidduk. In term of DPPH radical scavenging activity, the 5% or more addition level Sulgidduk showed an antioxdative effect increase of more than 50%. At the 3% or higher addition level, there was higher antioxidant activity than in the positive control group, which included vitamin C and BHT additions. We thus concluded that Sulgidduk with added ramie leaf power can be manufactured as a food that can satisfy sensory preferences as well as boosting antioxidant activity, at both the 3% and 5% addition level.

• International Journal of Advanced Culture Technology
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• 제6권2호
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• pp.100-106
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• 2018

Sometimes, literary therapy is to create an abscission layer at the mind and remove the signifier of love, by the abscission layer. The purpose of this study is to reveal the mechanism of literary therapy by mutation in human-created sentences and to activate literary therapy. In Gosijo, the abscission layer of Seo Gyeongdok's mind is present in the layer of the "falling leaf" of the his Sijo's last sentence, and the abscission layer of Hwang Jin-yi's mind exists in the layer of the "falling leaf" of the her Sijo's last sentence. The "falling leaf" created by the abscission layer of Seo Gyeongdok's sentence acts as an action potential to the nervous system of Hwang Jin-yi. In Hwang Jin-yi's Sijo, the abscission layer is also activated, forming a "leaf". Hwang Jin-yi's Sijo constructs a new sensory layer that is extended and mutated more than the meaning of space between lines of Seo Gyeongdok's Sijo. In her Sijo, Hwang Jin-yi twists and twists Seo Gyeongdok's sentences to squeeze the sound. She accepts the Seo Gyeongdok's sentences and twists in the first sentence and twists out of the second sentence once more to produce the mutation in the last sentence. As a result of her sentence twist, Seo Kyung-duk's visual sense of "falling leaf" is mutated into a "falling leaf's sound" of auditory sense. This mutation process works as a healing mechanism in the human body. If this study will continue in the future, it will be possible to activate literary therapy by Sijo.

• 식물조직배양학회지
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• 제26권1호
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• pp.59-63
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• 1999

국화 '남전'의 기내배양된 엽절편으로부터 신초 기관형성을 통한 효율적인 식물체 재생 체계를 확립하였다. 엽절편으로부터의 부정지 형성은 생장조절제 조성, 잎의 연령, 광조건, 배양용기당 절편체 수 및 치상방향에 의해 많은 영향을 받는 것으로 나타났다. 효율적인 부정지 형성을 위해서는 MS배지에 생장조절제 BA 0.5 ㎎/L 와 NAA 2.0 ㎎/L를 첨가하는 것이 가장 좋았으며, 엽절편체는 완전히 전개된 신초 상부의 1∼2번째 잎을 채취하여 4면을 절단한 다음 잎의 향축면이 배지에 닿도록 하여 Petri-dish당 8개의 엽절편을 치상하여 배양초기 1주간 암조건에서 배양하다가 명조건으로 옮겨주는 것이 가장 효과적이었다. 이와 같은 조건에서 엽절편체로부터 신초 재생률 100%, 절편체당 신초수 13.7개의 양호한 재생 이 이루어졌다. 재생된 신초는 NAA 0.1 ㎎/L를 첨가한 MS배지에서 성공적으로 발근이 이루어져 소식물체가 얻어졌다. 엽절편체로부터 얻어진 소식물체를 인공토양 (Vermiculite: Perlite=1:1)에서 활착시켜 온실에서 재배하였는데, 외견상 정상적으로 개화하였다.

• International Journal of Industrial Entomology and Biomaterials
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• 제35권2호
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• pp.111-117
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• 2017

Mulberry leaves containing 1-deoxynojirimycin (DNJ) known to be a strong inhibitory effect for ${\alpha}-glucosidase$. Thus, DNJ has been recognized as a potentially important source for prevent or treat hyperglycemia. More effective method for the DNJ high-production is needed because DNJ content of natural mulberry leaf are as low as 0.1%. Many researchers have studied for the DNJ high-production in mulberry leaves such as the harvest season, fermentation using microorganisms, optimal culture conditions, and optimal extraction conditions. In order to provide for useful data that is anticipated at the level of industrial scale, we investigated ${\alpha}-glucosidase$ inhibitory activity, pH value and DNJ content in large-scale based on the optimal culture conditions for mulberry leaf fermentation of small-scale in our previous study. The ${\alpha}-glucosidase$ inhibitory activity, pH value, and DNJ content in this study were measured from the mulberry leaf fermentation broth for 7 days. During mulberry leaf fermentation, the ${\alpha}-glucosidase$ inhibitory activity and DNJ content was increased until 2 to 4 days, but after 4 day was decreased. The pH value showed a decreasing trend up to 2 day, and little changes in 2 to 4 days. However, the pH was started to increase after 4 days.

• 한국식생활문화학회지
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• 제21권2호
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• pp.202-208
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• 2006

The effects of Lotus (Nelumbo nucifera Gaertner) leaf dry powder on serum lipid concentrations were evaluated in rats. Forty-eight male Sprague-Dawley rats were divided into six groups and fed high fat diets for sis weeks. Experimental groups were following diets; Control group (CON), 40 % animal and plant high fat group (AHF and PHF) control with 2% Lotus leaf dry powder group (CLDP), animal and plant high fat diets with 2% Lotus leaf powder group (AHFLDP, PHFLDP). Tissue weights of liver, heart, kidney, spleen and lung of AHF group exposed rats were decreased by PHFLDP groups. The concentrations of serum triglyceride in rats fed the CLDP and PHFLDP group were lower than those in other groups. The concentrations of total cholesterol in CLDP and PHFLDP group the were lower than those in AHF groups. The concentrations of HDL-cholesterol in serum of the CLDP and PHFLDP groups were significantly higher than those of other groups. The levels of LDL-cholesterol in serum of the CLDP and PHFLDP groups were tended to be lower than those of other groups. GPT and GOT were decreased in CLDP and PHFLDP groups and than in the AHF group. LDHase was lower in CLDP and PHFLDP groups than in the AHF group. These results suggest that 2% Lotus leaf dry powder groups may reduce elevated levels of serum lipid concentrations in rats fed high fat.

• 한국약용작물학회지
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• 제14권2호
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• pp.92-95
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• 2006

A method for plant regeneration via organogenesis from Pelagonium inquinans leaf disc has been developed. Mature leaf explants were collected from field-grown plants and used for the induction of adventitious shoot regeneration on Murashige and Skoog (MS) medium supplemented with 3% (w/v) sucrose plus plant growth regulators. Maximum shoot organogenesis, with $11.8{\pm}1.5$ shoots (98.6%) per leaf disc, was obtained with $2\;mg/l$ $N^6-benzyladenine$ (BA) and $0.5\;mg/l$ ${\alpha}-naphthyleneacetic$ acid (NAA) in 30 days. For rooting, the in vitro proliferated and elongated shoots were excised into 1.5-2 cm in length microcutting, which were plated individually on an half-strength MS (1/2MS) medium supplemented with 2% (w/v) sucrose plus various concentrations of indole-3-butyric acid (IBA). Shoots rooted with a frequency of 100% following culture on 1/2MS medium containing $0.5\;mg/l$ IBA.

• 식물병과 농업
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• 제1권2호
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• pp.18-21
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• 1995

Mycosphaerella nawae, the causal organism, of spotted leaf casing disease of persimmon, was isolated from infected leaves showing typical symptom. The cultural characteristics of the fungus were compared on artificial media. Among 24 different combinations of culture media and supplements, oatmeal agar+persimmon leaf extract (PLE) and PAD+ PLE+streptomycin showed the highest rate of isolating as 57.1%. The best medium for mycelial growth of the fungus was PDA+persimmon leaf powder (PLP). The colony diameter was reached 47mm for 30 days at 2$0^{\circ}C$. PDA+PLE also supported good mcyelial growth showing 46mm of diameter in same condition. The optimum growth temperature of this fungus in PDA was recognized fairly low. The mycelial growth was higher at 2$0^{\circ}C$ than 15$^{\circ}C$. The variation of pH between pH 4 to pH 8 did not affect to the mycelial growth of the pathogen.

• KSBB Journal
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• 제28권6호
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• pp.408-414
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• 2013

In order to obtain functional materials from aloe callus, we cultured Aloe vera L. leaf on MS medium added 0.2 mg/L IAA, 0.3 mg/L kinetin and 100 mg/L grape or/and apple juice for 30 days. While a callus differentiation during callus culture did not show, the cultured leaves were uniquely released extracellular material into the agar plate. After cultivation for 18 days, the cultured leaf and agar were harvested for extraction a functional material. The materials extracted were measured on the amount of total phenols, flavonoids and polysaccharides and determined on the antioxidant and antimicrobial activity. In result, callus extracts of additive free (CT) and added apple juice (2T) had more amount of phenol compound ($659{\mu}g/mL$, $533{\mu}g/mL$) and flavonoid ($580{\mu}g/mL$, $501{\mu}g/mL$) than natural leaf (p: $525{\mu}g/mL$, f: $301{\mu}g/mL$). However, the extract of natural leaf had the better effect of lipid peroxidation and polysaccharide content than the culture extract. All samples extracted had same effect on the nitrite scavenging activity. On the other hand, only 2T extract showed excellent 72% nitric oxide scavenging activity. The agar extract was also confirmed to contain polyphenol compound and polysaccharide content that had antioxidant and antimicrobial activity partly.

• 한국축산시설환경학회지
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• 제16권3호
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• pp.215-226
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• 2010

본 연구는 목편살수여상 pilot 장치를 설치하여 스크레파 축사에서 배출되는 뇨오수를 원수로 사용하여 가동한 후 침출액비를 공시재료로 하여 상추 양액재배 시험을 실시하였다. 처리구는 침출액비 (SL) 단독처리구, 침출 액비+어분 (SL+FM), 침출액비+골분 (SL+BM), 침출액비+게껍질 (SL+CS) 혼합처리구. 침출 액비+부산물 (SL+FM+BM+CS) 혼합처리구, 대조구로 원예연 상추양액 (NS) 처리구 등 6개 처리구를 두었다. 침출액비와 부산물 혼합처리 후 전기전도도와 pH를 조정하여 상추의 양액재배를 실시한 결과를 요약하면 다음과 같다. 1. 목편살수여상 처리 과정중 pH는 처리 시작전 7.3에서 처리 후 8.5로 상승하였다. 전기전도도 (EC)는 처리전 17.05 mS/cm에서 처리 후 30일에는 9.7 mS/cm로 낮아졌다. 2. 침출액비는 부유물질 (SS)이 낮아 수경 재배시 관배수의 막힘문제 없이 활용이 가능하였다. 침출액비는 양분조성에서 칼륨함량이 높고 인산 함량이 낮았다. 3. 침출액비시용구 (SL)의 상추수량은 원예 연 표준양액재배 대비 75%를 나타내어 25% 수량저하를 나타내었다. 침출액비을 어분. 골분. 게껍질을 혼합한 처리구는 대조구 대비 85%. 79%. 80% 수량을 나타내어 어분의 첨가 효과가 가장 높았다. 침출액비와 어분 혼합액은 침출액비 단독처리구 보다 10% 증수를 나타내어 유기양액의 대체 원료로 사용이 가능 할 것으로 보인다.