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Studies on the Lipid Components of Potato Tubers - III. Composition of Polarlipids in Free and Bound Lipids - (감자의 지방질(脂肪質) 성분(成分)에 관한 연구(硏究) - 제(第) 3 보(報) : 유리(遊離) 및 결합(結合) 지질(脂質)중의 극성(極性) 지질(脂質)의 조성(組成)에 관하여 -)

  • Lee, Sang-Young;Shin, Hyo-Sun
    • Korean Journal of Food Science and Technology
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    • v.11 no.4
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    • pp.304-313
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    • 1979
  • The compositions of the polar lipids in the free and bound lipids from four varieties of experimentally cultivated potatoes were identified and quantified by thin layer- and gas-liquid chromatographies. The results were summarized as follows : 1. The glycolipids contained in the free and bound lipids were fractionated and identified as esterified sterol glycoside, monogalactosyl diglyceride, sterol glycoside, digalactosyl diglyceride, and trigalactosyl diglyceride, of which the highest content to the total lipid quantity were 5.8% of esterified sterol glycoside in the free lipid and 6.1% of trigalactosyl diglyceride in the bound lipid. The content of monogalactosyl diglyceride in the free and bound lipids was almost the same, whereas the content of esterified sterol glycoside was higher in the free lipid than in the bound lipid, and the contents of sterol glycoside, digalactosyl diglyceride, and trigalactosyl diglyceride were higher in the bound lipid than in the free lipid on the contrary. 2. The phospholipid contained in the free and bound lipids were fractionated and identified as phosphatidyl ethanolamine, phosphatidyl glyceride, phosphatidyl choline, and phosphatidyl inositol, but the phosphatidyl glyceride was not detected in the free lipid. The highest content in the total lipid quantity was 3.3 % of phosophatidyl choline in the case of the free lipid, while 14.9 % of the phosphatidyl ethanolamine contained in the bound lipid was the highest. All other constituents of phospholipid were contained in larger quantity in the bound lipid than in the free lipid. 3. The fatty acid composition of glycolipid in the free and bound lipids was also the same as that of the total free and bound lipids. The differences were that the content of palmitic acid was higher in the glycolipid of the free lipid than in the total free lipid and the content of linoleic acid was lower in the glycolipid.

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Effect of adhesive hydrophobicity on microtensile bond strength of low-shrinkage silorane resin to dentin (접착시스템의 소수성이 Low-shrinkage silorane resin과 상아질의 미세인장강도에 미치는 영향)

  • Cho, So-Yeun;Kang, Hyun-Young;Kim, Kyoung-A;Yu, Mi-Kyung;Lee, Kwang-Won
    • Restorative Dentistry and Endodontics
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    • v.36 no.4
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    • pp.280-289
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    • 2011
  • Objectives: The purpose of this study was to evaluate ${\mu}TBS$ (microtensile bond strength) of current dentin bonding adhesives which have different hydrophobicity with low-shrinkage silorane resin. Materials and Methods: Thirty-six human third molars were used. Middle dentin was exposed. The teeth were randomly assigned to nine experimental groups: Silorane self-etch adhesives (SS), SS + phosphoric acid etching (SS + pa), Adper easy bond (AE), AE + Silorane system bonding (AE + SSb), Clearfil SE bond (CSE), CSE + SSb, All-Bond 2 (AB2), AB2 + SSb, All-Bond 3 (AB3). After adhesive's were applied, the clinical crowns were restored with Filtek LS (3M ESPE). The 0.8 mm ${\times}$ 0.8 mm sticks were submitted to a tensile load using a Micro Tensile Tester (Bisco Inc.). Water sorption was measured to estimate hydrophobicity adhesives. Results: ${\mu}TBS$ of silorane resin to 5 adhesives: SS, 23.2 MPa; CSE, 19.4 MPa; AB3, 30.3 MPa; AB2 and AE, no bond. Additional layering of SSb: CSE + SSb, 26.2 MPa; AB2 + SSb, 33.9 MPa; AE + SSb, no bond. High value of ${\mu}TBS$ was related to cohesive failure. SS showed the lowest water sorption. AE showed the highest solubility. Conclusions: The hydrophobicity of adhesive increased, and silorane resin bond-strength was also increased. Additional hydrophobic adhesive layer did not increase the bond-strength to silorane resin except AB2 + SSb. All-Bond 3 showed similar ${\mu}TBS$ & water sorption with SS. By these facts, we could reach a conclusion that All-Bond 3 is a competitive adhesive which can replace the Silorane adhesive system.

Analysis on the Growth Environment of Chionanthus retusus Community at the Wansanchielbong in Jeonju (전주 완산칠봉 이팝나무 자생지의 생육환경으로 본 자연유산 가치 분석)

  • Kim, Yeon
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.28 no.4
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    • pp.85-97
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    • 2010
  • This study analyzed the distribution, structure and environmental condition of the vegetation of the Chionanthus retusus Lindly et Paxton community at the Wansanchielbong in the Jeonju city to offer basic data for sustainable conservation and ecological management system. And the results are as follows; 1. The average pH of soil at the community was pH 5.69 and it was slightly higher than the average of forest soil pH of Korea. But if the degree of pH will be down, it will be needed some more fertilization of Calcium. 2. The total average for contents of organism was 4.98%. And the nitrate - nitrogen content(mg/kg) of A, B, C, D quadrat was 20.29%, 28.87%, 7.65%, and 23.3% respectively. And there were good condition except quadrat C which was contaminated by amount of earth and sand. 3. The flora of the Chionanthus retusus Lindly et Paxton community was listed as 60 taxa; 37 families, 50 genera, 47 species, 10 varieties and 3 forms. The average appearance species of each Quadrat were A sector 30, B sector 26, C sector 19 and D 19 taxa respectively. 4. Surveyed woody plants in the community were as follows : Chionanthus retusus, Zelkova serrata, Quercus variabilis, Cornus walteri, Robinia pseudo-acacia and those were mixed status. And Chionanthus retusus, Zelkova serrata, Robinia pseudo-acacia, Albizzia julibrisin, Cudrania tricuspidata, Symplocos chinensis for. pilosa were mixed in mid layer trees. Herbaceous plants were founded such as Chionanthus retusus, Zelkova serrata, Robinia pseudo-acacia, Grewia parviflora, Rosa multiflora, Trachelospermum asiaticum was dominant with 35~64% in the ground cover, and Commelina communis, Calamagrostis arundinacea, Dryopteris bissetiana, Lilium lancifolium were founded also. 5. The importance values of Chionanthus retusus was 40.2% in the quadrat A1, 50.2% at quadrat A, 50.0% B1, 45.2% B2, 22.4% C1, 73.6% C2, 33.2% D1 and the total average of I.V. was 44.9%. 6. The average height of surveyed Chionanthus retusus was 5.7m and the average DBH was 12.4cm. The number of trees higher than 2m were 107 and the number of trees lower than 2m were 63. The total numbers of Chionanthus retusus were 170. 7. The age of surveyed Chionanthus retusus were analyzed 42 thru 87 years old and that of Zelkova serrata were 42, Quercus variabilis were 60, Quercus aliena were 48, Robinia pseudo-acacia were 40. 8. The number of trees with DBH 40 through 50cm were 6, and that of 30~39cm were 3, and that of 20~29cm were 16, so the total number that was over 20cm was 25. And there were 70 trees under 10cm of DBH and 63 seedlings. It will be very important data to conserve the habitat that the structure and environmental condition of the Chionanthus retusus Lindly et Paxton community at the Wansanchielbong was stable, and sustainable monitoring will be needed. Now that community is nurse forest of Jeonju City but more positive preservation plan will be needed and assigning monument of city or province also be necessary.

Case Study of Hydrochemical Contamination by Antimony Waste Disposal in Korea (국내 안티몬폐기물에 의한 수질화학적 오염 사례연구)

  • Jeong, Chan-Ho
    • The Journal of Engineering Geology
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    • v.18 no.4
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    • pp.471-482
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    • 2008
  • This study was carried out to investigate the contamination characteristics of surface water, soil water and groundwater around and in antimony waste landfill site in Wonsung-ri, Yeonki-kun, Chungnam. The waste disposed in the study was excavated and transported to the other site in several years ago. For this study, we collected 35 water samples including groundwater, soil water and surface in the study site and also collected 2 groundwater samples from a comparison site. The data of chemical analysis of soil water samples show the antimony concentration of $48.75{\sim}74.81\;ppb$, which is much higher than groundwater in a comparison site and is highly excess than regulation level for a drinking water of some advanced countries. A relatively high antimony concentration was detected in three stream water samples nearby landfill site and two groundwater samples. Fe and Mn contents in soil water and stream water were measured as maxium 6.5 mg/L and 7.3 mg/L, respectively. Although other heavy metals of water samples in the study site are higher concentration than water sample of comparison site, their absolute levels are lower than regulation level for a drinking water. The chemical data of water samples are plotted widely from Ca - $HCO_3$ type to Ca - ($Cl +SO_4+NO_3$) type. Some groundwater show high contents of potassium and nitrate, which would come from fertilizer and sewage. Conclusively, some heavy metals including antimony have been still remained under the soil surface of the landfill site in the past. These metals have leaked out into nearby stream and groundwater system, and threaten the ecology, crops and the health of residents in this site. Therefore, the government have to prepare the strategy to prevent the diffusion of heavy metals into aquatic environment and have to process the reclamation work for contaminated site. It is also necessary to make a regulation level of the antimony concentration for a drinking water and soil environment in Korea.

Structural Features of Various Trichomes in Vitex negundo during Development (방향성 좀목형(Vitex negundo)모용의 구조적 분화발달)

  • Lee, Seung-Hee;Kim, In-Sun
    • Applied Microscopy
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    • v.36 no.1
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    • pp.35-45
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    • 2006
  • Plants of Vitex negundo are known to develop numerous trichomes throughout their body, where certain trichome types have been believed to be one of the plausible structures for the unique scents. In the current study. structural aspects of the trichomes have been examined in leaves and stems of Vitex negundo using TEM and SEM. Trichome types as well as structural changes that occurred in certain trichomes during secretion have been mainly focused. Three type of glandular trichomes and two types of non-glandular trichomes were developed in the epidermis of young and mature Vitex negundo plants. The glandular trichomes included the peltate type (Type 1), the capitate type (Type 2), and degraded capitate type (Type 3), whereas the non-glandular warty trichomes contained the multicellular (Types 4) and unicellular type (Type 5). Type 1 and 2 consisted of head and stalk cells, but their number and size were different. One secretory cavity was formed from the four head cells in the former, but only two head cells were involved in the latter. The cytoplasmic density in the head cell was quite high and in particular, sER and Golgi bodies were well developed. At initiation of their development, the cuticle layer of the head cells separated from the outer tangential wall to form a secretory cavity. Subsequently the cavity expanded acropetally and a large number of secretory vesicles continuously produced from the head cells until they filled the entire cavity. The cavity contained materials that would be soon discharged into intercellular spaces and/or into the air. The cavity began to decrease the volume by contracting at initial secretion but degrade rapidly within short time. It has been suggested that the mode of secretion in V. negundo is probably the eccrine secretion, since no break or rupture of the cavity has been observed during examination. Contrastingly Type 3 exhibited deterioration of the head cell at early stage. Type 4 was about $110{\sim}190{\mu}m$ long, consisting of $2{\sim}3$ cells, and distributed more in the adaxial epidermis compared to the abaxial surface. However, $20{\sim}30{\mu}m$ long Type 5 was extremely dense in both epidermis. Among several trichome types, Type 1 and 2 probably play an important role in discharging unique aromatic scents in plants of V. negundo.

Changes in Fatty Acid Composition of Grain after Milling (곡류 도정에 따른 지방산 조성 변화 연구)

  • Cho, Young-Sook;Kim, Yu-Na;Kim, Su-Yeonk;Kim, Jung-Bong;Kim, Heon-Woong;Kim, Se-Na;Kim, So-Young;Park, Hong-Ju;Kim, Jae-Hyun
    • Korean Journal of Environmental Agriculture
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    • v.30 no.4
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    • pp.409-413
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    • 2011
  • BACKGROUND: Cereals, especially rice is the staple food of oriental nations and because it is very important for Korean food, to determine the extent of nutrient losses due to milling, we analyzed the fatty acid using by GC-FID(Gas Chromatography-Flame Ionization Detector). Experimented rice cereals were rice, glutinous rice, Heuinchalssalbori, Seodunchalbori, Saessalbori, Keunalbori No.1, barnyard millet produced in Korea. METHODS AND RESULTS: After milling, the contents of fatty acids in the rice, glutinous rice, Heuinchalssalbori, and Seodunchalbori, Keunalbori No.1 rather decreased, but in the Saessalbori, and barnyard mille increased. Particularly, fatty acid content of the rice decreased from 24.8 mg/g to 6.4 mg/g, glutinous rice decreased from 29.4 mg/g to 11.7 mg/g after milling. There were also significant changes in the compositions of fatty acid among samples. Stearic acid ($C_{18:0}$) increased from 5% to 15%, but oleic acid ($C_{18:1}$) and linoleic acid ($C_{18:2}$) decreased in the rice after milling. CONCLUSION(s):In the brown rice, 11 different types of fatty acids were detected, and its highest content was found in grains. However, milled grain was observed only seven fatty acids in the case of rice. This result insisted that a portion of the lipid layer was significantly lost during the milling operation in rice.

Effects of Restricted Feeding during Growing Period on Growth and Endocrine Profile in Layers (산란계의 육성기 제한급여가 체성장 특성 및 내분비 변화에 미치는 영향 미치는 영향)

  • Kang, Hwan Ku;Cho, Jae Hoon;Kim, Ji Hyuk;Kang, Geun Ho;Yu, Dong Jo;Na, Jae Cheon;Kim, Dong Wook;Lee, Sang Jin;Kim, In Shik;Kim, Sang Ho
    • Journal of Animal Science and Technology
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    • v.50 no.3
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    • pp.343-354
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    • 2008
  • The objective of this study was to investigate the effect of restricted feeding to pullet on growth and endocrine profile in layers(Isa brown). One thousand eighty brown-layer chicks were divided into three treatments; conventional feeding(ad libitum) and two restricted feedings(80% of conventional diet) for 70 weeks. One of restricted feeding started from seven to seventeen weeks of age, and the other started from twelve to seventeen weeks of age. Diets were formulated by NRC(1994) recommendation. Body weight and feed intake were measured every two weeks, and body composition and organ weight analyzed every week in rearing period and every eight weeks in laying period. Blood was collected at weighing body weight and was analyzed for the concentration of insulin-like growth factor-Ⅰand estradiol. Body weight and feed intake decreased as restriction of feeding begins comparing to ad libitum group(P<0.05), but reached to similar body weight and feed intake at 20 wk of age regardless of restriction. There were no significant differences in development of digestive tract and attached organs among the treatment groups. During rearing period, however, they were slightly higher in restricted group which was low in body weight. In terms of body composition, fat contents of restricted group were significantly lower(P<0.05) than that of ad libitum group around 1,000 grams of body weight. High concentration of IGF-Ⅰwas observed.

EFFECT OF THE EXPONENTIAL CURING OF COMPOSITE RESIN ON THE MICROTENSILE DENTIN BOND STRENGTH OF ADHESIVES (복합레진의 exponential 중합법이 상아질접착제의 미세인장접착강도에 미치는 영향)

  • Seong, So-Rae;Seo, Duck-kyu;Lee, In-Bog;Son, Ho-Hyun;Cho, Byeong-Hoon
    • Restorative Dentistry and Endodontics
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    • v.35 no.2
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    • pp.125-133
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    • 2010
  • Objectives: Rapid polymerization of overlying composite resin causes high polymerization shrinkage stress at the adhesive layer. In order to alleviate the shrinkage stress, increasing the light intensity over the first 5 seconds was suggested as an exponential curing mode by an LED light curing unit (Elipar FreeLight2, 3M ESPE). In this study, the effectiveness of the exponential curing mode on reducing stress was evaluated with measuring microtensile bond strength of three adhesives after the overlying composite resin was polymerized with either continuous or exponential curing mode. Methods: Scotchbond Multipurpose Plus (MP, 3M ESPE), Single Bond 2 (SB, 3M ESPE), and Adper Prompt (AP, 3M ESPE) were applied onto the flat occlusal dentin of extracted human molar. The overlying hybrid composite (Denfil, Vericom, Korea) was cured under one of two exposing modes of the curing unit. At 48h from bonding, microtensile bond strength was measured at a crosshead speed of 1.0 mm/min. The fractured surfaces were observed under FE-SEM. Results: There was no statistically significant difference in the microtensile bond strengths of each adhesive between curing methods (Two-way ANOVA, p > 0.05). The microtensile bond strengths of MP and SB were significantly higher than that of AP (p < 0.05). Mixed failures were observed in most of the fractured surfaces, and differences in the failure mode were not observed among groups. Conclusion: The exponential curing method had no beneficial effect on the microtensile dentin bond strengths of three adhesives compared to continuous curing method.

Organization and function of shoot apical meristem affecting growth and development in plants (식물의 생장과 발달에 영향을 미치는 슈트 정단분열조직의 체제와 기능)

  • Lee, Kyu Bae
    • Journal of Plant Biotechnology
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    • v.41 no.4
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    • pp.180-193
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    • 2014
  • In plants, a shoot apex has a small region known as the shoot apical meristem (SAM) having a group of dividing (initiating) cells. The SAM gives rise to all the groundabove structures of plants throughout their lifetime, and thus it plays important role in growth and development of plants. This review describes theories to explain the SAM organization and function developed over the last 250 years. Since in 1759 German botanist C. F. Wolff has described firstly the SAM, in 1858 Swiss botanist C. N${\ddot{a}}$geli proposed the apical cell theory from the observation of a large single apical cell in the SAM of seedless vascular plants: however, this view was recognized to be unsuitable to seed plants. In 1868, German botanist J. Hanstein suggested the histogen theory: this concept subdividing the SAM into dermatogen, periblem, and plerome was unable to generally apply to seed plants. In 1924, German botanist A. Schmidt proposed the tunica-corpus theory from the examination of angiosperm SAM in which two parts show different planes of cell division: this theory was proved to be not suitable to gymnosperm SAM, not have stable surface tunica layer. In 1938, American botanist A. Foster described zones in gymnosperm SAM based on the cytohistologic differentiation and thus called it a cytohistological zonation theory. With works by E. Gifford, in 1954, this zonation pattern was demonstrated to be also applicable to angiosperm SAM. As another theory, in 1952 French botanist R. Buvat proposed the m${\acute{e}}$rist${\grave{e}}$me d'attente (waiting meristem) theory: however, this concept was confuted because of its negation of function during vegetative growth phase to central initial cells. Rescent studies with Arabidopsis thaliana have found that formation and maintenance of the SAM are under the control of selected genes: SHOOTMERISTEMLESS (STM) gene forms the SAM, and WUSCHEL (WUS) and CLAVATA (CLV) genes function in maintaining the SAM; signaling between WUS and CLV genes act through a negative feedback loop.

The influence of composite resin restoration on the stress distribution of notch shaped noncarious cervical lesion A three dimensional finite element analysis study (복합레진 수복물이 쐐기형 비우식성 치경부 병소의 응력 분포에 미치는 영향에 관한 3차원 유한요소법적 연구)

  • Lee, Chae-Kyung;Park, Jeong-Kil;Kim, Hyeon-Cheol;Woo, Sung-Gwan;Kim, Kwang-Hoon;Son, Kwon;Hur, Bock
    • Restorative Dentistry and Endodontics
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    • v.32 no.1
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    • pp.69-79
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    • 2007
  • The purpose of this study was to investigate the effects of composite resin restorations on the stress distribution of notch shaped noncarious cervical lesion using three-dimensional (3D) finite element analysis (FEA). Extracted maxillary second premolar was scanned serially with Micro-CT (SkyScan1072 ; SkyScan, Aartselaar, Belgium). The 3D images were processed by 3D-DOCTOR (Able Software Co., Lexington, MA, USA). ANSYS (Swanson Analysis Systems, Inc., Houston, USA) was used to mesh and analyze 3D FE model. Notch shaped cavity was filled with hybrid or flowable resin and each restoration was simulated with adhesive layer thickness ($40{\mu}m$) A static load of 500 N was applied on a point load condition at buccal cusp (loading A) and palatal cusp (loading B). The principal stresses in the lesion apex (internal line angle of cavity) and middle vertical wall were analyzed using ANSYS. The results were as follows 1. Under loading A, compressive stress is created in the unrestored and restored cavity. Under loading B, tensile stress is created. And the peak stress concentration is seen at near mesial corner of the cavity under each load condition. 2. Compared to the unrestored cavity, the principal stresses at the cemeto-enamel junction (CEJ) and internal line angle of the cavity were more reduced in the restored cavity on both load con ditions. 3. In teeth restored with hybrid composite, the principal stresses at the CEJ and internal line angle of the cavity were more reduced than flowable resin.