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시판 단감 와인농축물의 항산화 활성 및 신경세포 보호효과 (Antioxidant Activity and Neuroprotective Effect of Concentrates from Commercial Sweet Persimmon Wine)

  • 서혜경;장선영;김현정;박해룡;박중협;안광환;이승철
    • 한국식품조리과학회지
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    • 제26권1호
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    • pp.13-17
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    • 2010
  • 단감 와인을 농축한 후, 농도별로 녹여 추출물을 얻었다. 이 추출물을 이용하여 단감 와인의 항산화 활성을 조사하였다. 단감 와인의 총 페놀 함량(TPC)을 조사한 결과 $9.29{\pm}0.11\;mg/g$의 페놀을 함유하였다. DPPH 라디칼 소거능과 환원력 그리고 ABTS 라디칼 소거능은 농도 의존적으로 활성을 보였으며, $IC_{50}$ 값이 각각 2.959 mg/mL, 1.437 mg/mL 그리고 0.484 mg/mL으로 L-ascorbic acid 보다 낮은 활성을 보였다. 그리고, 단감 와인 농축물은 $10\;{\mu}g/mL$의 농도에서 무처리구에 비해 glutamate 독성으로부터 뇌신경계 세포주 hybridoma N18-RE-105세포를 22% 이상 보호하는 효과를 나타내었다.

Vitrification solution without sucrose for cryopreservation in mouse blastocysts

  • Joo, Jong Kil;Lee, Young Ju;Jeong, Ju Eun;Kim, Seung Chul;Ko, Gyoung Rae;Lee, Kyu Sup
    • Clinical and Experimental Reproductive Medicine
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    • 제41권3호
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    • pp.115-119
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    • 2014
  • Objective: This study was designed to investigate the survival rate of vitrified mouse blastocysts depending on the presence or absence of sucrose in vitrification solution. Methods: Mouse two-cell embryos were collected and cultured to blastocysts. Two vitrification solutions were prepared. The control solution was composed of 25% glycerol, 25% ethylene glycol, and 0.5 M sucrose (G25E250.5S) containing 2.5 mL glycerol, 2.5 mL ethylene glycol, 2 mL SSS, and 0.855 g sucrose in 5 mL PB1. The experimental solution was composed of 25% glycerol and 25% ethylene glycol (G25E25) and contained 2.5 mL glycerol and 2.5 mL ethylene glycol in 5 mL PB1. Artificial shrinkage was conducted by aspirating the blastocoelic fluid using an ICSI pipette. To examine the effect of sucrose in the vitrification solution on the survival rate of mouse blastocysts, the shrunken-equilibrated blastocysts were rehydrated or vitrified after being exposed to one of the two vitrification solutions. After exposure and the vitrification-thawing process, the re-expansion rate and hatching rate were evaluated after 6 hours of in vitro culture. Results: The re-expansion rate of mouse blastocysts exposed to vitrification solution with and without sucrose were not different in the experimental solution (without sucrose) (98%) and the control solution (with sucrose) (92%) (p>0.05). The hatching rate was higher in the experimental solution (95%) than in the control solution (88%), but did not differ across two treatments (p>0.05). The re-expansion rate of mouse blastocysts vitrified in the control solution was 92% and 94%, respectively (p>0.05), and the hatching rate was higher in the experimental solution (90%) than in the control solution (74%) (p<0.05). Conclusion: Sucrose need not be added in vitrification solution for freezing of artificially shrunken mouse blastocysts.

Change of Fibrinolytic and Antioxidative Activities of Codonopsis lanceolata According to Various Storage Conditions, and Heat or Salt Treatments

  • ;;김준호
    • 대한의생명과학회지
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    • 제11권1호
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    • pp.63-69
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    • 2005
  • We investigated the changes of fibrinolytic and antioxidative activities of wild or cultivated Codonopsis lanceolata, which were packed in woven polypropylene (WP) film or low density polyethylene (LDPE) film and stored for 0, 15, and 30 days at refrigerated $(2\~4^{\circ}C)$ or room $(l8\~20^{\circ}C)$ temperature (So we have 16 samples, and wrote them as the abbreviated words such as W-WP-RE-15, W-WP-RO-15, W-LDPE-RE-15, W-LDPE-RO-15, W-WP-RE-30, W-WP-RO-30, W-LDPE-RE-30, W-LDPE-RO-30, C-WP-RE-15, C-WP-RO-15, C-LDPE-RE-15, C-LDPE-RO-15, C-WP-RE-30, C-WP-RO-30, C-LDPE-RE-30, C-LDPE-RO-30). Fibrinolytic activity of fresh Codonopsis lanceolata cultivated in the mountain or field were 0.8 unit (plasmin unit/ml) or 7.3 units, respectively. In descending order, the activities of wild Codonopsis lanceolata stored for 15 days were as followed; LDPE-RE (0.70 unit), WP-RO (0.52 unit), WP-RE (0.45 unit), and LDPE-RO (0.30 unit). After 30 days, fibrinolytic activities of them decreased to 0.47 unit (LDPE-RE), 0.28 unit (WP-RO), 0.21 unit (WP-RE), and 0.30 unit (LDPE-RO). Considering from the point of fibrinolytic activity, the optimal storage condition of wild Codonopsis lanceolata was packing with LDPE film and storing at $4^{\circ}C$. The change of fibrinolytic activities of Codonopsis lanceolata cultivated in the field revealed the similar trend as wild samples, but the fibrinolytic activities of 30 days-stored samples were maintained better than the wild Codonopsis lanceolata. Fibinolytic activity of wild Codonopsis lancealata was increased by heating for 5 min at $100^{\circ}C$ and decreased by addition of NaCl. Antioxidative activities of Codonopsis lanceolata were also compared from the electron donating activity. Fresh Codonopsis lanceolata had about $70\%$ of electron donating activity. Independent of cultivation area, electron donating activity dropped to $19\~74\%$ (wild Codonopsis lanceolata) and $27\~59\%$ (cultivated Codonopsis lanceolata) during 15 days storage. But after 30 days storage, we obtained the unexpected results, which meant that the activities were higher activities than 15 days-stored samples or even though the fresh samples. In general, Codonopsis lanceolata could maintain antioxidative activities most strongly with LDPE film and chilled condition.

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GENERALIZED EULER POWER SERIES

  • KIM, MIN-SOO
    • Journal of applied mathematics & informatics
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    • 제38권5_6호
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    • pp.591-600
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    • 2020
  • This work is a continuation of our investigations for p-adic analogue of the alternating form Dirichlet L-functions $$L_E(s,{\chi})={\sum\limits_{n=1}^{\infty}}{\frac{(-1)^n{\chi}(n)}{n^s}},\;Re(s)>0$$. Let Lp,E(s, t; χ) be the p-adic Euler L-function of two variables. In this paper, for any α ∈ ℂp, |α|p ≤ 1, we give a power series expansion of Lp,E(s, t; χ) in terms of the variable t. From this, we derive a power series expansion of the generalized Euler polynomials with negative index, that is, we prove that $$E_{-n,{\chi}}(t)={\sum\limits_{m=0}^{\infty}}\(\array{-n\\m}\)E_{-(m+n),{\chi}^{t^m}},\;n{\in}{\mathbb{N}}$$, where t ∈ ℂp with |t|p < 1. Some further properties for Lp,E(s, t; χ) has also been shown.

암독소 호르몬-L이 유발하는 체지방 분해작용에 고려인삼의 각 진세노사이드 성분이 미치는 영향 (Effect of Ginsenosides of Red Ginseng on Lipolytic Action of Toxohormone-L from Cancerous Ascites Fluid)

  • 이성동;황우익
    • Journal of Ginseng Research
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    • 제15권2호
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    • pp.106-111
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    • 1991
  • This study was devised to obserL'e the inhibitory effects of 7 kinds of ginsenosides on a lipolytic action of Toxohormone-L. The ginsenosides used in this experiment were -Rbl, -Rbl, - Rc, - Re, - Rgl, and - Rg2 prepared from Korean red ginseng. Toxohormene-L was partially purified by centrifigation from the ascites fluids of Sarcoma-180 bearing mice. In vitro test showed that the inhibitory effect of - Rb2 on the lipolysis by Toxohormone-L was highest percent among other treatments at concentration of 100 $\mu\textrm{g}$/ml and 500 $\mu\textrm{g}$/ml of reaction mixture. And total inhibitory activity (units) of - Rb2 was also highest among other treatments at the same concentration. However, in vivo test, body weight gain of Sarcoma-180 bearing mice decreased significantly by administration of - Rg2 compared to those of the control or other ginsenosides treated groups.

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THE ASYMPTOTIC BEHAVIOUR OF THE AVERAGING VALUE OF SOME DIRICHLET SERIES USING POISSON DISTRIBUTION

  • Jo, Sihun
    • East Asian mathematical journal
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    • 제35권1호
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    • pp.67-75
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    • 2019
  • We investigate the averaging value of a random sampling of a Dirichlet series with some condition using Poisson distribution. Our result is the following: Let $L(s)={\sum}^{\infty}_{n=1}{\frac{a_n}{n^s}}$ be a Dirichlet series that converges absolutely for Re(s) > 1. If $X_t$ is an increasing random sampling with Poisson distribution and there exists a number $0<{\alpha}<{\frac{1}{2}}$ such that ${\sum}_{n{\leq}u}a_n{\ll}u^{\alpha}$, then we have $${\mathbb{E}}L(1/2+iX_t)=O(t^{\alpha}{\sqrt{{\log}t}})$$, for all sufficiently large t in ${\mathbb{R}}$. As a result, we get the behaviour of $L({\frac{1}{2}}+it)$ such that L is a Dirichlet L-function or a modular L-function, when t is sampled by the Poisson distribution.

수직축을 중심으로 회전하는 직관과 정지한 곡관내의 유동 유사성에 관한 연구 (Study on the Similarity of Flows in an Orthogonally Rotating Square Duct and a Stationary Curved Square Duct)

  • 이공희;백제현
    • 대한기계학회:학술대회논문집
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    • 대한기계학회 2001년도 춘계학술대회논문집E
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    • pp.825-830
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    • 2001
  • A numerical study on a quantitative analogy of the fully developed flow between in a straight square duct rotating about an axis perpendicular to that of the duct and a stationary curved duct of square cross-section is carried out. In order to clarify the similarity of two turbulent flows, the dimensionless parameters $K_{TR} = Re^{1/4}/\sqrt{Ro}$ and the Rossby number, Ro, in a rotating straight duct flow were used as a set corresponding to $K_{TC} = Re^{1/4}/\sqrt{{\lambda}}$ and curvature ratio, ${\lambda}$, in a stationary curved duct flow so that they have the same dynamical meaning as $K_{LR} = Re/\sqrt{Ro}$ and $K_{LC} = Re/\sqrt{{\lambda}}$ of the fully developed laminar flows. For the large values of Ro or A, it is shown that the flow field satisfies the asymptotic invariance property: there are strong quantitative similarities between the two flows such as flow patterns and friction factors for the same values of $K_L$ and $K_T$.

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Apical membrane antigen-1 (AMA-1) gene sequences of re-emerging Plasmodium vivax in South Korea

  • Han, Eun-Taek;Park, Jae-Hwan;Shin, Eun-Hee;Choi, Min-Ho;Oh, Myoung-Don;Chai, Jong-Yil
    • Parasites, Hosts and Diseases
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    • 제40권3호
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    • pp.157-162
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    • 2002
  • Plasmodium vivax malaria re-emerged in South Korea in 1993, and epidemics continue since then. We examined genetic variation in the region encompassing the apical membrane antigen-1 (PvAMA-1) of the parasites by DNA sequencing of the 22 re-emerging p. vivax isolates. The genotype of the PvAMA-1, which was based on sequence data previously reported for the polymorphic regions, showed that two haplotypes were present at one polymorphic site. Compared with reported data, the two types, SKOR type I and type II, were similar to Chinese CH- l0A and CH-05A isolates, respectively. Thus, the present study showed that two genotypes of AMA-1 genes coexist in the re-emerging Korean P. vivax.

Manufacturing Regenerated Woody Dyed Fiber from Waste MDF Using Natural Dyes

  • JU, Seon-Gyeong;ROH, JeongKwan
    • Journal of the Korean Wood Science and Technology
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    • 제48권2호
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    • pp.154-165
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    • 2020
  • To assign the functionality of the regenerated fiber from waste MDF(wMDF) made of pitch pine, we examined the dyeing characteristics of natural dyes, sappan wood as a polychromatic natural red series, monochromatic gardenia as a yellow series, and indigo blue series. For nonemordanting dye, the colors of regenerated fiber dyed by sappan wood and gardenia were reddish yellow (YR) and yellow (Y) series, respectively, and dyeing conditions were appropriate a 30 ~ 50 g/L of dyeing materials at 60 ℃ for 60minutes of dyeing time. We obtained regenerated woody dyed fibers (Re-WDF), YR to the red (R) series by premordanting with Al and Cu mordant for sappan wood and the purplish red (RP) series by Fe premordanting. In the case of gardenia, only Y series colors were developed in nonemordanting dye or all three mordants. Indigo dye produced Re-WDF with greenish yellow (GY) tone at 1%, green (G) tone at 3%, and blue (B) tone at 5% concentration or more. Re-WDF with indigo showed the best light fastness followed by sappan wood and gardenia. In particular, the light fastness of Re-WDF with gardenia was very poor. The light fastness was somewhat improved by premordanting(Fe>Cu>Al) both sappan wood and gardenia dyes.

[ $C^1$ ] Continuous Piecewise Rational Re-parameterization

  • Liang, Xiuxia;Zhang, Caiming;Zhong, Li;Liu, Yi
    • International Journal of CAD/CAM
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    • 제6권1호
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    • pp.59-64
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    • 2006
  • A new method to obtain explicit re-parameterization that preserves the curve degree and parametric domain is presented in this paper. The re-parameterization brings a curve very close to the arc length parameterization under $L_2$ norm but with less segmentation. The re-parameterization functions we used are $C^1$ continuous piecewise rational linear functions, which provide more flexibility and can be easily identified by solving a quadratic equation. Based on the outstanding performance of Mobius transformation on modifying pieces with monotonic parametric speed, we first create a partition of the original curve, in which the parametric speed of each segment is of monotonic variation. The values of new parameters corresponding to the subdivision points are specified a priori as the ratio of its cumulative arc length and its total arc length. $C^1$ continuity conditions are imposed to each segment, thus, with respect to the new parameters, the objective function is linear and admits a closed-form optimization. Illustrative examples are also given to assess the performance of our new method.