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고추의 Tobamovirus 저항성 L 유전자좌와 연관된 대립유전자 특이적인 마커 세트 (A Set of Allele-specific Markers Linked to L Locus Resistant to Tobamovirus in Capsicum spp.)

  • 이준대;한정헌;윤재복
    • 원예과학기술지
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    • 제30권3호
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    • pp.286-293
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    • 2012
  • 고추에 있어서 Tobamovirus 저항성은 고추 염색체 11번 긴 팔 끝부분에 위치한 L 유전자좌의 다섯 개 대립유전자($L^0$, $L^1$, $L^2$, $L^3$, and $L^4$)에 의해 조절된다고 알려져 있다. 표현형 분석 없이 L 대립유전자를 구분할 수 있는 분자표지를 개발하기 위해서 다섯 개의 고추 판별 계통{Capsicum annuum Early California Wonder(ECW, $L^0L^0$), C. annuum Tisana($L^1L^1$), C. annuum Criollo de Morelos 334(CM334,$L^2L^2$), Capsicum chinense PI 159236($L^3L^3$), and Capsicum chacoense PI 260429($L^4L^4$)}을 식물재료로 사용하였다. 대립유전자 특이적 분자표지는 고추 판별 계통에 대해 $L^3$ 연관 분자표지(189D23M, A339, and 253A1R)와 BAC 염기서열(FJ597539 and FJ597541)의 PCR 증폭산물 염기서열을 비교 분석하여 개발되었다. 총 53개의 상용 고추 품종 중 48개에서 분자표지에 의한 추정 유전자형과 Tobamovirus{Tobacco mosaic virus(pathotype 0, $P_0$), Tomato mosaicvirus($P_1$), and Pepper mild mottle virus($P_{1,2}$)} 접종 표현형과 일치했다. 결과적으로 본 연구에서 개발된 분자표지는 고추 육종에 있어서 TMV 저항성 도입에 필요한 선발마커로 충분히 활용될 수 있을 것이다.

열간압연으로 제조된 STS 304L/A516-70N과 STS 316L/A516-70N 클래드재들의 미세조직에 대한 비교 연구 (Comparative Study on Microstructures of Hot-rolled STS 304L/A516-70N and STS 316L/A516-70N Clad Plates)

  • 진주찬;조수철;심호섭;이영국
    • 열처리공학회지
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    • 제34권4호
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    • pp.171-178
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    • 2021
  • In the present study, we comparatively investigated the microstructures of two hot-rolled stainless steel clad plates; STS 304L - low carbon steel A516-70N and STS 316L - A516-70N. The STS 304L/A516-70N clad plate (Clad_304L_Ni) had a Ni-interlayer between stainless steel and carbon steel and a 90 ㎛ thick deformation band of unrecrystallized austenite grains on the stainless steel. The STS 316L/A516-70N clad plate (Clad_316L) had no interlayer and almost fully recrystallized austenite grains. Clad_304L_Ni exhibited the thinner a decarburized layer in carbon steel and a total carburized layer in stainless steel than Clad_316L. However, a severely carburized layer in stainless steel was thicker for Clad_304L_Ni than Clad_316L. Hardness profiles near the interface of clad plates matched well with microstructures at locations where the hardness values were measured.

주간 및 야간 시간대에 따른 $L_{dn}$의 상관관계 - 도로교통소음을 중심으로 - (The Correlation of $L_{dn}$ in accordance with the daytime and the nighttime - Focusing on road traffic noise -)

  • 김득성;장서일;이연수
    • 한국소음진동공학회:학술대회논문집
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    • 한국소음진동공학회 2006년도 추계학술대회논문집
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    • pp.34-40
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    • 2006
  • The daytime(7 a.m to 10 p.m.) and the nighttime(10 p.m. to 7 a.m.) used to calculate existing $L_{dn}$ is different from the domestic daytime(6 a.m to 10 p.m.) and nighttime(10 p.m. to 6 a.m.) periods. The difference of a time periods makes too difficult for converting measured $L_{eq}$ during daytime($L_d$) and nighttime($L_n$) periods to $L_{dn}$. Thus, it is difficult to directly compare with $L_{dn}$ standard of a foreign country. The pupose of paper is to propose a proper experimental equations that make up for the problems. The data of this paper used road traffic noise data of Auto-Network System(ANS) that generates $L_{eq}$ TNI, $L_{NP}$ for 1 hour. A method of this paper is as follows.(1) The data of ANS converted 24 hour $L_{eq}$ which measured every 1 hour to existing $L_{dn}$ and to $L_{dn}$ of an experimental equations.(2) The existing Lan is compared to results of $L_{dn}$ from experimental equations. The paper proposes a three experimental equations. This paper select an approximate equation that was most similar, to existing $L_{dn}$ out of these equations. When $L_{eq}$ data of different daytime and nighttime periods are converted to $L_{dn}$, an experimental equation of this paper can be used and applied to $L_{dn}$'s calculation.

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L-fuzzy ideals of a poset

  • Alaba, Berhanu Assaye;Taye, Miheret Alamneh;Engidaw, Derso Abeje
    • Annals of Fuzzy Mathematics and Informatics
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    • 제16권3호
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    • pp.285-299
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    • 2018
  • Many generalizations of ideals of a lattice to an arbitrary poset have been studied by different scholars. In this paper, we introduce several L-fuzzy ideals of a poset which generalize the notion of an L-fuzzy ideal of a lattice and give several characterizations of them.

Listeria monocytogenes Serovar 4a is a Possible Evolutionary Intermediate Between L. monocytogenes Serovars 1/2a and 4b and L. innocua

  • Chen, Jianshun;Jiang, Lingli;Chen, Xueyan;Luo, Xiaokai;Chen, Yang;Yu, Ying;Tian, Guoming;Liu, Dongyou;Fang, Weihuan
    • Journal of Microbiology and Biotechnology
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    • 제19권3호
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    • pp.238-249
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    • 2009
  • The genus Listeria consists of six closely related species and forms three phylogenetic groups: L. monocytogenes-L. innocua, L. ivanovii-L. seeligeri-L. welshimeri, and L. grayi. In this report, we attempted to examine the evolutionary relationship in the L. monocytogenes-L. innocua group by probing the nucleotide sequences of 23S rRNA and 16S rRNA, and the gene clusters lmo0029-lmo0042, ascB-dapE, rplS-infC, and prs-ldh in L. monocytogenes serovars 1/2a, 4a, and 4b, and L. innocua. Additionally, we assessed the status of L. monocytogenes-specific inlA and inlB genes and 10 L. innocua-specific genes in these species/serovars, together with phenotypic characterization by using in vivo and in vitro procedures. The results indicate that L. monocytogenes serovar 4a strains are genetically similar to L. innocua in the lmo0035-lmo0042, ascB-dapE, and rplS-infC regions and also possess L. innocua-specific genes lin0372 and lin1073. Furthermore, both L. monocytogenes serovar 4a and L. innocua exhibit impaired intercellular spread ability and negligible pathogenicity in mouse model. On the other hand, despite resembling L. monocytogenes serovars 1/2a and 4b in having a nearly identical virulence gene cluster, and inlA and inlB genes, these serovar 4a strains differ from serovars 1/2a and 4b by harboring notably altered actA and plcB genes, displaying strong phospholipase activity and subdued in vivo and in vitro virulence. Thus, by possessing many genes common to L. monocytogenes serovars 1/2a and 4b, and sharing many similar gene deletions with L. innocua, L. monocytogenes serovar 4a represents a possible evolutionary intermediate between L. monocytogenes serovars 1/2a and 4b and L. innocua.

EDI 방식과 XML 방식간 통합관리를 통한 전자신용장의 활성화 방안에 관한 연구 (A Study on the Integrated Management of XML and EDI Electronic Letters of Credit)

  • 장상식;안병수
    • 무역상무연구
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    • 제58권
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    • pp.237-263
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    • 2013
  • Traditionally, a L/C (letter of credit) has been known as a relatively low risk method of payment. This is why L/C has been used in international trade. However, L/C has a number of weak points such as high cost, long processing time, and complicated documents. Using an electronic L/C is one way to solve those weak points. In Korea, there are two types of electronic L/C. One is the EDI (Electronic Data Interchange) based L/C and the other one is the XML (Extensible Markup Language) based L/C. The former, established in 1990's, is sent from banks to the beneficiary solely through VAN (Value Added Network, KTNET) operators. On the other hand the latter, started in 2005, is sent from banks to KFTC (Korea Financial Telecommunications & Clearings Institute) for management of the L/C balance, as well as to KTNET. So far, paper L/C and EDI based L/C have been used overwhelmingly instead of XML based L/C in spite of the aforesaid disadvantages. In this paper, the authors examined empirically why the users of electronic L/C were reluctant to use XML based L/C. The results are as follows. First, the users of paper L/C were more dissatisfied than the users of electronic L/C due to many factors such as cost, the time required, and information reuse. Second, the users who have more experience with XML based L/C wanted to adopt integrated management with EDI based L/C more than the users who had not experienced XML based L/C. Third, the users who had used more than one form of L/C wanted to adopt integrated management to EDI and XML based L/C more than the users who had only used one form of L/C. Therefore, the authority for electronic L/C should consider a change of the policy from the XML based electronic L/C oriented to integrated management of the various types of L/C.

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MA 316L ODS 및 Wet 316L ODS 스테인리스강에서 충격에너지에 미치는 소결 공정의 영향 (Effects of the Sintering Variable on Impact Energy in MA 316L ODS and Wet 316L ODS Stainless Steels)

  • 김성수;한창희;장진성
    • 한국분말재료학회지
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    • 제17권2호
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    • pp.113-122
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    • 2010
  • Two kinds of oxide-dispersion-strengthened (ODS) 316L stainless steel were manufactured using a wet mixing process(wet) and a mechanical alloying method (MA). An MA 316L ODS was prepared by a mixing of metal powder and a mechanical alloying process. A wet 316L ODS was manufactured by a wet mixing with 316L stainless steel powder. A solution of yttrium nitrate was dried after being in the wet 316L ODS alloy. The results showed that carbon and oxygen were effectively reduced during the degassing process before the hydroisostatic process (HIP) in both alloys. It appeared that the effect of HIP treatment on increase in impact energy was pronounced in the MA 316L ODS alloy. The MA 316L ODS alloy showed a higher yield strength and a smaller elongation, when compared to the wet 316L ODS alloy. This seemed to be attributed to the enhancement of bonding between oxide and matrix particles from HIP and to the presence of a finer oxide of about 20 nm from the MA process in the MA 316L ODS alloy.

Korean Secondary School Students' L2 Learning Motivation: Comparing L2 Motivational Self System with Socio-educational Model

  • Kim, Yoon-Kyoung;Kim, Tae-Young
    • 영어어문교육
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    • 제18권1호
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    • pp.115-132
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    • 2012
  • In order to confirm ecological validity of D$\ddot{o}$rnyei's second language motivational self, the present study investigated 495 South Korean secondary school students' L2 learning motivation and motivated behavior by using a questionnaire survey. The participants' ideal L2 self, ought-to L2 self, integrativeness, and instrumentality were examined and compared to identify which motivational factor had the most effect on their motivated L2 learning behavior. Among Korean secondary school students, the concept of integrativeness was replaced with the ideal L2 self as a more appropriate concept for understanding Korean secondary school students' L2 learning motivation. As for instrumentality, promotional aspects demonstrated a significant correlation with both the ideal and ought-to L2 selves, whereas preventional aspects showed a relatively high correlation with the ought-to L2 self. The ideal L2 self had the most impact on the motivated behavior, and the ideal L2 self and ought-to L2 self reflected the students' different motivational characteristics. It is suggested that D$\ddot{o}$rnyei's (2005, 2009) L2 motivational self system can be a better psychological model than Gardner's (1985) socio-educational model for understanding Korean secondary school students' L2 learning motivation.

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Development of a UPLC-MS/MS method for the therapeutic monitoring of L-asparaginase

  • Jeong, Hyeon-Cheol;Kim, Therasa;Yang, Deok-Hwan;Shin, Kwang-Hee
    • Translational and Clinical Pharmacology
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    • 제26권3호
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    • pp.134-140
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    • 2018
  • This study aimed to develop a UPLC-MS/MS method for determining plasma levels of L-aspartic acid and L-asparagine and the activity of L-asparaginase. L-aspartic acid, L-asparagine, and L-aspartic acid-2,3,3-$d_3$ were extracted from human plasma by protein precipitation with sulfosalicylic acid (30%, v/v). The plasma samples were analyzed using an Imtakt Intrada amino acid analysis column with 25 mM ammonium formate and 0.5% formic acid in acetonitrile as the mobile phase with step gradient method at a flow rate of 0.5 mL/min. The injection volume was $5{\mu}L$, and the total run time was 15 min. Inter- and intra-batch accuracies (%) ranged from 96.62-106.0% for L-aspartic acid and 89.85-104.8%, for L-asparagine, and the coefficient of variation (CV%) did not exceed 7%. The validation results for L-aspartic acid and L-asparagine satisfied the specified criterion, however, the results for L-asparaginase activity assay showed a borderline validity. This study could be a foundation for further development of therapeutic drug monitoring systems using UPLC-MS/MS.

Some Fundamental Concepts in (2, L)-Fuzzy Topology Based on Complete Residuated Lattice-Valued Logic

  • Zeyada, Fathei M.;Zahran, A.M.;El-Baki, S.A.Abd;Mousa, A.K.
    • International Journal of Fuzzy Logic and Intelligent Systems
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    • 제10권3호
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    • pp.230-241
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    • 2010
  • In the present paper we introduce and study fundamental concepts in the framework of L-fuzzifying topology(so called(2,L)-fuzzy topology)as L-concepts where L is a complete residuated lattice. The concepts of (2,L)-derived, (2,L)-closure, (2,L)-interior, (2,L)-exterior and (2,L)-boundary operators are studied and some results on above concepts are obtained. Also, the concepts of an L-convergence of nets and an L-convergence of filters are introduced and some important results are obtained. Furthermore, we introduce and study bases and subbases in (2,L)-topology. As applications of our work the corresponding results(see[10-11]) are generalized and new consequences are obtained.