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Effect of Treadmill Exercise Training on the Expression of PGC-1α, GLUT-1, Tfam Proteins and Antioxydent Ezymes in Brain of STZ-Induced Diabetic Rats (트레드밀 지구성 운동이 streptozotocin으로 유발된 당뇨 흰쥐의 뇌에서 PGC-1α, GLUT-1, Tfam 단백질 및 항산화 효소(Cu, Zn-SOD, Mn-SOD)의 발현량에 미치는 영향)

  • Park, Noh-Hwan;Lee, Jin;Jung, Kook-Hyun;Choi, Bong-Am;Jang, Hyung-Chae;Lee, Suk-In;Lee, Dong-Soo;Cho, Joon-Yong
    • Journal of Life Science
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    • v.21 no.3
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    • pp.435-443
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    • 2011
  • The purpose of this study is to identify the effects of exercise training [ET, 10~18 m/min (speed), 20~30 min (exercise duration)/a day for 5 day/wk, 6 wk) on PGC-$1{\alpha}$, GLUT-1, Tfam, Cu,Zn-SOD and Mn-SOD proteins in brain of STZ-induced diabetic rats. The male Sprague-Dawley (SD) rats were single-injected intraperitoneally with 50mg/kg of streptozotocin (STZ) to produce STZ-induced diabetic rats. Rats were divided into 3 experimental groups with 8 rats in each group, as follows: (1) non-STZ group (n=8), (2) STZ-CON group (n=8), (3) STZ-EXE group (n=8). The results of this study suggest that i) serum glucose level was significantly reduced in STZ-EXE group compared with STZ-CON group (p<0.05), ii) PGC-$1{\alpha}$ (p<0.001), mtPGC-$1{\alpha}$ (p<0.001), GLUT-1 (p<0.001), and mtTfam (p<0.001) proteins in brain of STZ-induced diabetic rats were significantly increased in STZ-EXE group compared with STZ-CON group, iii) Cu,Zn-SOD (p<0.001) and Mn-SOD (p<0.01) proteins in the STZ-induced diabetic rats were significantly increased in STZ-EXE group compared with STZ-CON group. In conclusion, the findings of the present study reveal that treadmill exercise training increases brain GLUT-1 protein level possibly through up-regulation of PGC-$1{\alpha}$ and Tfam proteins which represent key regulatory components of stimulation of brain mitochondrial biogenesis. In addition, treadmill exercise training may prevent oxidative stress by up-regulation of Cu,Zn-SOD and Mn-SOD proteins in the STZ-induced diabetic rats.

Effect of Silicate on Creeping Bentgrass Growth of Green at the Golf Course during Summer in Korea (규산염의 시비가 크리핑 벤트그래스의 여름철 생육에 미치는 영향)

  • Lee, Jae-Pil;Yoo, Tae-Young;Moon, Se-Jong;Ham, Suon-Kyu;Kim, Doo-Hwan
    • Asian Journal of Turfgrass Science
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    • v.22 no.2
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    • pp.217-223
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    • 2008
  • This study was conducted to figure out the effect of silicate as growth stimulator on growth of 'Pencrosss' creeping bentgrass. Creeping bentgrass(Agrostis palustris cv. 'Pencross') at the nursery of Sinwon Country Club was used. Silicate was applied at the concentration of 0, $200{\times}$, $500{\times}$, $1.000{\times}$. Polt size was 1 by 2 meter and there were three replications with completely randomized design(CRD). Creeping bentgrass growth was evaluated with visual turf grass quality, root length and No. of tiller density(ea/$cm^2$). Results of this study are as followings; 1. Average root length with silicate was $1.5{\sim}1.9cm$ longer than control. Especially, Root length of silicate was $7{\sim}8cm$ in summer. 2. Tiller density with silicate was $l8{\sim}22ea/cm^2$, $0.4{\sim}2.l$ less than control. But there was no significant difference. 3. Visual turfgrass quality with silicate was $5.0{\sim}8.3$, $0.3{\sim}1.5$ higher than control. But there was no significant difference. In conclusion, silicate might be grown as root growth stimulator of creeping bentgrass during summer in Korea. However, this study was conducted under one year. Accordingly, in-depth experiment should be done over several years.

A Study on the Policy Directions of Sports Welfare in Gangwon Province for Improving Quality of Life (삶의 질 향상을 위한 강원도 스포츠복지 정책방향 연구)

  • Kim, Heung-Tae;Kim, Tae-Dong
    • Journal of Korea Entertainment Industry Association
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    • v.13 no.8
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    • pp.411-424
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    • 2019
  • The purpose of this study is to explore the feasible policy directions for sports welfare that can not only improve the standard of living through health but also ensure a happy and enjoyable life for the people of Gangwon Province. For this purpose, I have conducted studies such as the analysis on the sports class voucher project being implemented by the South Korean government and the case analysis in sports welfare, and present policy directions as follows. First of all, it is about upgrading the sports class voucher project. And as its implementation plans, I suggest ① increased publicity, ② the earmarking of the province's own budget for the sports class voucher project, ③ the establishment of a system for cooperation for work implementation between the related organizations and their staff in charge with a view to activating the sports class voucher project, and ④ the upgraded services for the sports class voucher project and the upgraded access to the life cycle-based universal welfare. Second, it is about using public sports facilities and developing various programs. I suggest the active utilization of the public sports facilities that enable people to learn the skills for such sports disciplines as baseball, badminton, ice sports, and golf and the development and distribution of distinctive educational programs for dance for media entertainment shows for female youths, climbing, cheer leading, fencing, surfing and horseback riding. Third, it is about nurturing the human resources and networking. For this, I suggest the creation of 'Sports Welfare Specialist Training Program' and the training of the college students majoring in sports science with the aim of creating a number of jobs. Fourth, it is about refurbishing the system and establishing the support system. I suggest the dismantling of the partitions in the welfare policy related to sports activities and the formation of (tentatively named) 'Gangwon Province Sports Welfare Implementation Committee', and the creation of (tentatively named) 'Sports Welfare Project Support Team' in Health, Welfare & Women's Affairs Bureau or Culture, Tourism and Sports Bureau in the short term and then its long-term expansion into (tentatively named) 'Gangwon Province Sports Welfare Support Center' in responding to the needs that reflect the provincial demographics, with a view to establishing a single lineup for the administrative support system. Furthermore, as budget and manpower are needed to realize customized sports welfare that suits the characteristics of the province and in which all the provincial residents can collect benefits, I suggest that the province provide the legal basis through creating 'Ordinance Promoting Sports Welfare in Gangwon Province' and pushing forward with (tentatively named) 'Gangwon Province Sports Welfare Competition' as what revises the sports class voucher project for the purpose of broadening the basis for sports welfare promotion.

Comparison of Germination Characteristics and Daily Seed Germinating Pattern in 8 New Cultivars of Perennial Ryegrass Grown under Alternative and Natural Room Temperature Conditions (자연실온 및 변온환경에서 퍼레니얼 라이그래스 신품종 8종류의 발아특성 및 일일 발아패턴 비교)

  • Kim, Kyoung-Nam
    • Asian Journal of Turfgrass Science
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    • v.24 no.2
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    • pp.79-87
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    • 2010
  • Research was initiated to investigate the early establishment characteristics and germination patterns of perennial ryegrass (PR, Lolium perenne L.). Eight cultivars of PR were evaluated in the study. Experiment was conducted under a room temperature condition of $6^{\circ}C$ to $23^{\circ}C$ (natural conditions). Daily and cumulative germination patterns were measured and analyzed on a daily basis. Significant differences were observed in germination pattern, days to the first germination, days to 50% germination, days to 75% germination, and germination percentage with different environments and cultivars. Germination percentage was variable with cultivars at the end of study. It was 66.25 to 93.50% under natural conditions. There were considerable variations in the early germination characteristics and germination pattern among the entries according to different conditions. The first germination was initiated between 3 and 5 DAS (days after seeding) under ISTA (International Seed Testing Association) conditions. But it started between 6 and 8 DAS under natural conditions, being 2 to 4 days later as compared with that under ISTA conditions. It was 8 to 12 DAS that reached to days to the 50% germination, which was 2 to 5 days after the first germination. Days to the 75% germination were 10.08 to 13.70 DAS under natural conditions, being 2.07 to 5.70 days slower as compared with ISTA conditions. 'Pennant II' was the fastest cultivar. But the slowest was 'Catalina II' that was 3.62 days later than 'Pennant II' under natural conditions. Considering days to the first germination, days to 75% germination, and germination pattern etc, 'Pennant II' and 'Brightstar II' were regarded as excellent cultivars under both conditions. From this study, information on differences in germination characteristics and patterns would be usefully applied for golf course design and construction, when established with PR.

Comparison of Germination Characteristics and Daily Seed Germinating Pattern in New Cultivars of The Third Generation of Creeping Bentgrass Grown under Alternative and Natural Room Temperature Conditions (자연실온 및 변온조건에서 제3세대 크리핑 벤트그래스 신품종의 발아특성 및 일일 발아패턴 비교)

  • Kim, Kyoung-Nam;Kwon, O-Dal;Shim, Sang-Ryul;Yoon, Jung-Seo;Park, So-Hyang
    • Asian Journal of Turfgrass Science
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    • v.25 no.1
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    • pp.1-10
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    • 2011
  • This study was conducted to investigate early germination characteristics and germination pattern of new cultivars of creeping bentgrass (CB, Agrostis pall/stris Huds.) under natural room temperature conditions and to analyze differences between alternative and natural conditions. Seven cultivars of CB were tested in the study. Daily and cumulative germination patterns were measured and analyzed on a daily basis. Significant differences were observed in germination pattern, days to the first germination, days to 50% germination, days to 85% germination, and germination rate among cultivars under different environments. The first germination in all entries was initiated between 4 and 5 days after seeding (DAS) under alternative conditions, while between 6 and 8 DAS under natural conditions, being 2 to 3 days later as compared with that of alternative conditions. As for the first germination percentage, it was generally 5 to 25% under alternative conditions. However, 'Penn A-1', 'Penncross', and 'Penn A-4' cultivars showed a vigorous germination, producing 50 to 70%. Under natural room temperature conditions, it was 5 to 10% for the most entries, except 'Penncross', 'L-93', and 'Penn A-4' cultivars which vigorously produced 20 to 50%. Days to the 50% germination in CB resulted in 4 to 10 DAS under alternative conditions, while 6 to 15 DAS under natural conditions, being 2 to 5 days later than under alternative conditions. Days to the 85% germination were 5.90 to 11.75 DAS under alternative conditions and 7.95 to 12.50 DAS under natural conditions. 'Penn A-1' and 'L-93' were the fastest cultivars under alternative and natural conditions, respectively. Regardless of the temperature conditions, the longest cultivar in germination was 'T-1', being over 11 DAS. In-depth comparative data on germination characteristics and germination patterns among CB cultivars would be usefully applied for a practical CB turf establishment in golf course construction etc.

Effect of Polymer, Calcium, Perlite and Chitosan in Soil Organic Amendment on Growth in Perennial Ryegrass (유기질 토양개량재에서 고분자 중합체, 칼슘, 펄라이트 및 키토산이 퍼레니얼 라이그래스의 생장에 미치는 효과)

  • Kim, Kyoung-Nam
    • Asian Journal of Turfgrass Science
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    • v.26 no.1
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    • pp.24-34
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    • 2012
  • The study was carried out to investigate the effects of polymer, calcium, perlite and chitosan on the growth of perennial ryegrass (Lolium perenne L., PR) and to provide a basic information needed for their practical application when establishing garden, parks, athletic field and golf courses with these materials. A total of 24 treatment combinations were applied in the study. Treatments were made of water-swelling polymer (WSP), calcium, perlite and chitosan mixed in soil organic amendment (SOA). Germination rate, turfgrass coverage, turfgrass density and top growth were evaluated in PR under greenhouse conditions. Significant differences were observed for these growth characteristics among the treatments. Turfgrass density and plant height, evaluated on a weekly basis, varied with time after seeding. A proper mixing rate of WSP was considered to be lower 3% for the growth of PR with an exception of being below 6% for turfgrass density. Germination rate and early survival capacity were greatly influenced by calcium and chitosan among the elements of calcium, perlite, and chitosan. But there was little effect by perlite. Calcium and chitosan were most effective one for turfgrass density and coverage, respectively. Top leaf-growth was influenced by all three elements, but the greatest effect was highly linked with calcium. Chitosan was very effective in early germination and vertical leaf growth, as compared with the others. Future studies are required for measuring the effect of WSP, calcium, perlite and chitosan on the turf growth characteristics in root zone mixtures of sand+SOA before a practical field use.

Amendments and Construction Systems for Improving the Performance of Sand-Based Putting Greens (골프장 putitng green 개선을 위한 토양 개량제와 green 구조시설)

  • Ok Chang-Ho;Anderson Stephen H.;Ervin Erik H.
    • Asian Journal of Turfgrass Science
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    • v.18 no.3
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    • pp.149-163
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    • 2004
  • Physical and chemical properties of root zone mixes and methods of green construction are important considerations for improving turf grass quality for putting greens. This study compared Penncross creeping bentgrass (Agrostis palustris Huds.) performance as affected by three root zone construction systems with three amendments (sand, peat, and zeolite). The objective of this study was to determine if an amended California construction system would improve green performance during establishment (1998-1999) and maturation (2000-2001). Three treatments were tested: California ($100\%$ sand), USGA($90\%$ sand and $10\%$ peat, v/v), and California-Z ($85\%$ sand and $15\%$ zeolite, v/v). Treatments were arranged in a randomized complete block with four replicates. Physical and chemical properties of the root zone and bentgrass performance were compared for the treatments. The California-Z treatment had the highest saturated hydraulic conductivity, field infiltration rate and the lowest bulk density. It also had the highest cation exchange capacity and plant available nutrient concentrations among the three treatments. The California-Z treatment produced bentgrass quality and color during green establishment and maturation that were equal to or higher than the California treatment, and consistently higher than the USGA treatment. The addition of an inorganic amendment to the California system improved physical and chemical properties of the root zone and improved quality and color of bentgrass during green establishment. During green maturation, creeping bentgrass in the California-Z treatment was equal (6 of 15 sampling dates) or $20\%$ higher (9 of 15 dates) in quality compared to the California system.

The Effect of Compound Fertilizer Contained Slow Release Nitrogen on Turfgrass Growth in Kentucky bluegrasss and on Nitrogen Change in Root Zone (완효성질소성분이 함유된 복합비료의 시비가 토양 중 질소함량 변화 및 켄터키블루그래스 생육에 미치는 영향)

  • Kim, Young-Sun;Kim, Tack-Soo;Ham, Suon-Kyu;Bang, Su-Won;Lee, Chang-Eun
    • Asian Journal of Turfgrass Science
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    • v.23 no.1
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    • pp.101-110
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    • 2009
  • This study was conducted to investigate the effect of compound fertilizer contained slow release nitrogen such as isobutylidene diurea(IBDU) and methylene urea(MU) on kentucky bluegrasss and the change of nitrogen in the root zone after supplying these at AM turfgrass research institute with soil analysis, chlorophyll content index, leaf area index and dry weight during 6 months from July to December in 2007 year. Fertilizer treatments were designed as following; non-fertilizer(NF), straight fertilizer(CF), methylene urea(MU), isobutylidene diurea(IBDU). The every treatments were arranged a randomized complete block with three replications. Results obtained were summarized as follows: As relative to time after N fertilizers application, ammonium and nitrate in soil were increased, but T-N was decrease. The change of ratio of available Nand T-N by elapsed time after fertilizing was decreased in CF from 30days but increased until 45days. Compared with NF, the turf color index in CF, MU and IBDU was increased 1.5%, 2.5% and 2.3%, respectively, the chlorophyll content 16%, 25% and 26%, the dry weight 31%, 62% and 46% and shoot number 104%, 185% and 148%. The chlorophyll content index of MU and IBDU was increased 7.5% and 8.8% than that of CF, shoot number 40% and 22%, and dry weight 24% and 12% but turf color index was similar to that of CF. These results showed that the MU and IBDU application was supplied nitrogen for a long time in root zone by increasing an available nitrogen, and so promote turf grass growth such as chlorophyll content and shoot number in kentucky bluegrass.

The Problem of Space Debris and the Environmental Protection in Outer Space Law (우주폐기물과 지구 및 우주환경의 보호)

  • Lee, Young Jin
    • The Korean Journal of Air & Space Law and Policy
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    • v.29 no.2
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    • pp.205-237
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    • 2014
  • Last 50 years there were a lot of space subjects launched by space activities of many states and these activities also had created tremendous, significant space debris contaminating the environment of outer space. The large number of space debris which are surrounding the earth have the serious possibilities of destroying a satellite or causing huge threat to the space vehicles. For example, Chinese anti-satellite missile test was conducted by China on January 11, 2007. As a consequence a Chinese weather satellite was destroyed by a kinetic kill vehicle traveling with a speed of 8 km/s in the opposite direction. Anti-satellite missile tests like this,contribute to the formation of enormous orbital space debris which can remain in orbit for many years and could interfere with future space activity (Kessler Syndrome). The test is the largest recorded creation of space debris in history with at least 2,317 pieces of trackable size (golf ball size and larger) and an estimated 150,000 debris particles and more. Several nations responded negatively to the test and highlighted the serious consequences of engaging in the militarization of space. The timing and occasion aroused the suspicion of its demonstration of anti-satellite (ASAT) capabilities following the Chinese test of an ASAT system in 2007 destroying a satellite but creating significant space debris. Therefore this breakup seemed to serve as a momentum of the UN Space Debris Mitigation Guidelines and the background of the EU initiatives for the International Code of Conduct for Outer Space Activities. The UN Space Debris Mitigation Guidelines thus adopted contain many technical elements that all the States involved in the outer space activities are expected to observe to produce least space debris from the moment of design of their launchers and satellites until the end of satellite life. Although the norms are on the voluntary basis which is normal in the current international space law environment where any attempt to formulate binding international rules has to face opposition and sometimes unnecessary screening from many corners of numerous countries. Nevertheless, because of common concerns of space-faring countries, the Guidelines could be adopted smoothly and are believed faithfully followed by most countries. It is a rare success story of international cooperation in the area of outer space. The EU has proposed an International Code of Conduct for Outer Space Activities as a transparency and confidence-building measure. It is designed to enhance the safety, security and sustainability of activities in outer space. The purpose of the Code to reduce the space debris, to allow exchange of the information on the space activities, and to protect the space objects through safety and security. Of the space issues, the space debris reduction and the space traffic management require some urgent attention. But the current legal instruments of the outer space do not have any binding rules to be applied thereto despite the incresing activities on the outer space. We need to start somewhere sometime soon before it's too late with the chaotic situation. In this article, with a view point of this problem, focused on the the Chinese test of an ASAT system in 2007 destroying a satellite but creating significant space debris and tried to analyse the issues of space debris reduction.

Molecular Identification of Zoysia japonica and Zoysia sinica (Zoysia Species) Based on ITS Sequence Analyses and CAPS (ITS 염기서열 분석 및 CAPS를 이용한 조이시아 속(Zoysia) 들잔디와 갯잔디의 구별)

  • Hong, Min-Ji;Yang, Dae-Hwa;Jeong, Ok-Cheol;Kim, Yang-Ji;Park, Mi-Young;Kang, Hong-Gyu;Sun, Hyeon-Jin;Kwon, Yong-Ik;Park, Shin-Young;Yang, Paul;Song, Pill-Soon;Ko, Suk-Min;Lee, Hyo-Yeon
    • Horticultural Science & Technology
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    • v.35 no.3
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    • pp.344-360
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    • 2017
  • Zoysiagrasses are important turf plants used for school playgrounds, parks, golf courses, and sports fields. The two most popular zoysiagrass species are Zoysia japonica and Zoysia sinica. These are widely distributed across different growing zones and are morphologically distinguishable from each other; however, it is phenotypically difficult to differentiate those that grow along the coastal line from those in beach area habitats. A combination of morphological and molecular approaches is desirable to efficiently identify these two plant cultivars. In this study, we used a rapid identification system based on DNA barcoding of the nrDNA-internal transcribed spacer (ITS) regions. The nrDNA-ITS regions of ITS1, 5.8S nrDNA, and ITS2 from Z. japonica, Z. sinica, Agrostis stolonifera, and Poa pratensis were DNA barcoded to classify these grasses according to their molecular identities. The nrDNA-ITS sequences of these species were found at 686 bp, 687 bp, 683 bp, and 681 bp, respectively. The size of ITS1 ranged from 248 to 249 bp, while ITS2 ranged from 270 to 274 bp. The 5.8S coding region ranged from 163 - 164bp. Between Z. japonica and Z. sinica, nineteen (2.8%) nucleotide sites were variable, and the G+C content of the ITS region ranged from 55.4 to 63.3%. Substitutions and insert/deletion (indel) sites in the nrDNA-ITS sequence of Z. japonica and Z. sinica were converted to cleaved amplified polymorphic sequence (CAPS) markers, and applied to the Zoysia grasses sampled to verify the presence of these markers. Among the 62 control and collected grass samples, we classified three groups: 36 Z. japonica, 22 Z. sinica, and 4 Z. japonica/Z. sinica hybrids. Morphological classification revealed only two groups; Z. japonica and Z. sinica. Our results suggest that used of the nrDNA-ITS barcode region and CAPS markers can be used to distinguish between Z. japonica and Z. sinica at the species level.