• Asian-Australasian Journal of Animal Sciences
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• v.14 no.6
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• pp.880-884
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• 2001

Rumen microbiology research has undergone several evolutionary steps: the isolation and nutritional characterization of readily cultivated microbes; followed by the cloning and sequence analysis of individual genes relevant to key digestive processes; through to the use of small subunit ribosomal RNA (SSU rRNA) sequences for a cultivation-independent examination of microbial diversity. Our knowledge of rumen microbiology has expanded as a result, but the translation of this information into productive alterations of ruminal function has been rather limited. For instance, the cloning and characterization of cellulase genes in Escherichia coli has yielded some valuable information about this complex enzyme system in ruminal bacteria. SSU rRNA analyses have also confirmed that a considerable amount of the microbial diversity in the rumen is not represented in existing culture collections. However, we still have little idea of whether the key, and potentially rate-limiting, gene products and (or) microbial interactions have been identified. Technologies allowing high throughput nucleotide and protein sequence analysis have led to the emergence of two new fields of investigation, genomics and proteomics. Both disciplines can be further subdivided into functional and comparative lines of investigation. The massive accumulation of microbial DNA and protein sequence data, including complete genome sequences, is revolutionizing the way we examine microbial physiology and diversity. We describe here some examples of our use of genomics- and proteomics-based methods, to analyze the cellulase system of Ruminococcus flavefaciens FD-1 and explore the genome of Ruminococcus albus 8. At Illinois, we are using bacterial artificial chromosome (BAC) vectors to create libraries containing large (>75 kbases), contiguous segments of DNA from R. flavefaciens FD-1. Considering that every bacterium is not a candidate for whole genome sequencing, BAC libraries offer an attractive, alternative method to perform physical and functional analyses of a bacterium's genome. Our first plan is to use these BAC clones to determine whether or not cellulases and accessory genes in R. flavefaciens exist in clusters of orthologous genes (COGs). Proteomics is also being used to complement the BAC library/DNA sequencing approach. Proteins differentially expressed in response to carbon source are being identified by 2-D SDS-PAGE, followed by in-gel-digests and peptide mass mapping by MALDI-TOF Mass Spectrometry, as well as peptide sequencing by Edman degradation. At Ohio State, we have used a combination of functional proteomics, mutational analysis and differential display RT-PCR to obtain evidence suggesting that in addition to a cellulosome-like mechanism, R. albus 8 possesses other mechanisms for adhesion to plant surfaces. Genome walking on either side of these differentially expressed transcripts has also resulted in two interesting observations: i) a relatively large number of genes with no matches in the current databases and; ii) the identification of genes with a high level of sequence identity to those identified, until now, in the archaebacteria. Genomics and proteomics will also accelerate our understanding of microbial interactions, and allow a greater degree of in situ analyses in the future. The challenge is to utilize genomics and proteomics to improve our fundamental understanding of microbial physiology, diversity and ecology, and overcome constraints to ruminal function.

• Journal of the Korean association of regional geographers
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• v.13 no.1
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• pp.104-117
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• 2007

According to the evolution of knowledge based economy, the expansion of significance of high-tech and technical innovation, in recent, many of local government of Korea have concern on the establishment and promotion of new growth power such as technopolis, science park, and innovative clusters for regional development. This study focuses on the establishment strategy for R&D special district in Gwangju area. Gwangju area have many potentials in several dimensions; comfortable physical environment, new agglomeration of photonics and household electric industry, the existence of excellent research related manpower, the strong networking with universities, laboratories, and firms. In addition, the establishment of R&D special district in Gwangju area will be provide positive effects in the increase of competitiveness of state, balanced development between regions, revitalization and development of southwestern area, and establishment of innovative clusters for regional development. However, in order to promote and establish the R&D special district of Gwangju area, central and local governments have to concern with some improvements such as the construction of R&D related infrastructure, the strengthening of research activities of research institutes, the building of cluster of strategic industries of Gwangju area, the supporting system for the revitalization of R&D special district.

• Korean Medical Education Review
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• v.17 no.2
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• pp.78-87
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• 2015

Mongolia is suffering from the inadequate capacity of medical doctors due to a poor education and training system. The purpose of this article is to evaluate the effect of a clinical training program for capacity building of medical doctors in Mongolia, and to suggest an effective model for continuing professional development in developing countries. Based on the results of a needs assessment, Korean and Mongolian medical experts developed a clinical training program and trained the trainers on 32 topics regarding major clinical problems in 6 specialties, including cardiology, endocrinology, pediatrics, obstetrics and gynecology, neurology, and emergency medicine. Surveillance survey and pre/post-test were used on every topic to evaluate the satisfaction and achievement, respectively, of the trainees. Six months after the clinical training program, we interviewed a sample of medical professionals to evaluate the change and impact. A total of 612 (person-year) medical doctors participated in the training, and the average score for satisfaction was 7.69 out of 8. The average score of the pre-test was 46.9 out of 100, while the post-test was 82.4. After the training, the medical doctors were applying their new knowledge and skills to their practice, and using the materials as guidelines, which improved their practice and increased patient satisfaction. They also started their own training program and adopted new equipment at their hospitals. The satisfaction and achievement of the trainees were very high, and there was significant change in the medical practice, education system, and infrastructure after the training program. This training program can be an effective model for capacity building of medical doctors in developing countries.

• Korean Journal of Ichthyology
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• v.22 no.1
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• pp.9-16
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• 2010

Osmoregulatory capabilities of a euryhaline medaka, Oryzias dancena (Beloniformes; Teleostei), was examined with a particular emphasis on adult and larval viability during direct salinity changes. O. dancena adults were highly capable for hyper-osmoregulation as well as hypo-osmoregulation, as evidenced by no adverse effect on their viability during the direct transfer either from complete freshwater ($0^{\circ}/_{\circ\circ}$) to $40^{\circ}/_{\circ\circ}$ salinity, or from $70^{\circ}/_{\circ\circ}$ to $0^{\circ}/_{\circ\circ}$. Furthermore, the phased increase of external salinity with acclimation periods allowed them to survive at a salinity as high as $75^{\circ}/_{\circ\circ}$. However, tolerant capability to acute salinity increase in early larval stage was much less than in adult stage, based on the finding that the tolerance range of salinity increase was only $15^{\circ}/_{\circ\circ}$ from freshwater, indicating that the hyper-osmoregulation system might not be fully developed in the early larval stage. On the contrary, the hypoosmoregulation system could be more solidified in O. dancena larvae, as evidenced by their good survival even after direct transfer from $45^{\circ}/_{\circ\circ}$ to $0^{\circ}/_{\circ\circ}$. Knowledge achieved in this study could form the basis for a wide scope of researches including ecotoxicogenomics and geneexpression assay using this model species.

• Women's Health Nursing
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• v.1 no.1
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• pp.138-152
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• 1995

The condition of nutrition during infancy will greatly affect infants' physical, emotional growth, especially breast feeding is important in their growth and development, and emotional stability, too. Despite such advantages of breast-feeding, its rate has continued to fall year after year in Korea : the 95% rate in 1960's has fallen to 25.4% in 1990. It is known that such a downfall of breast-feeding rate is associated with various factors. The purpose of the study is to examine mother's and nurses' in nursery perception of the impediment factors of breast-feeding : to compare those between of breast-feeding : to compare those between two groups : to provide fundamental data for developing strategies for increasing breast-feeding. The subjects were 45 new mothers from one hospital and the same number of nurses sampled from 3 university hospitals, in Seoul. The data were collected for 11 days from April 12 to April 23, 1994 and a questionnaire was developed based on the interview with 14 nurses and 10 mothers and the literature reviews. Liker's Five-Point Scale was used as measurement. The Data were analyzed using SPSS / PC and descriptive statistics, t-test, ANOVA. The results of study are as follows : 1) Most new mothers get the information about breast-feeding from their mothers, books or newspaper, and relatives in rank than professionals(nurses or doctors). 2) The impediment factors the breast-feeding are categorized as mothers, hospital system, medical personnels and social factors. The most frequently mentioned impediment factors from mothers are 'Difficulty by operational wounds'(3.13), 'Lack of will for breast-feeding'(3.47), 'Insufficient rest and sleep'(3.52) and 'Opposition from husband or his family members'(4.77)in rank. On the other hand those factors from nurses are 'Inadequate nipple condition'(2.37), 'Decreasing milk secretion given medicine after operation'(2.63), 'Mothers knowledge deficit by poorly prepared education'(2.79) and 'Mothers abhorance of breast-feeing'(3.87) in rank. 3) As for the hospital system, the highly perceived impediment factors by mothers are 'Seperation of baby from mother after birth'(2.78), 'Lack of space for breast-feeding in the hospital'(2.93), 'Lack of facility for informing the time for breast-feeding'(3.18) and 'Because of babys' preference artificial nipple by being accustomed to it in hospital(3.97), in rank. Meanwhile, those from nurses are "Seperation of baby from mother after birth",(1.92), "Inconsistency between hospital's nursing time and mother's breast secretion time" scretion time(2.97), "Lack of space for breast-feeding in the hospital"(3.39), and "Lack of facility for informing the time for breast-feeding"(3.74) 4) As for the medical personnels, the highestly perceived Impediment factor from mothers in "Lack of professional nurses for breast-feeding"(2.96), and the lowestly perceived one is "Doctors' reluctance"(4.75). Nurses perceived same as mothers, too. 5) As for the social factors, the highestly perceived impediment factor by mothers and nurses is "Inconvience of social activities"(2.83) and the lowestly ones are "The sense of self-sacrifice"(4.22) by mothers, and "The sense of old fashioned"(4.13) by nurses. 6) The difference of the perception of impediment factors between mothers and nurses is statistically significant only in mother factor.

• Journal of KIISE:Computer Systems and Theory
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• v.33 no.12
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• pp.894-906
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• 2006

There are many difficulties to develop a mobile contents due to many constraints on mobile environments. It is difficult to make a good mobile contents with only visual reduction of existing contents on wire Internet. Therefore, it is essential to devise the data representation and to develop the authoring tool to meet the needs of the mobile contents market. We suggest the compact mobile contents to learn Chinese characters and developed its authoring tool. The animation which our system produces is realistic as if someone writes letters with pen or brush. Moreover, our authoring tool makes a user generate a Chinese character animation easily and rapidly although she or he has not many knowledge in computer graphics, mobile programming or Chinese characters. The method to generate the stroke animation is following: We take basic character shape information represented with several contours from TTF(TrueType Font) and get the information for the stroke segmentation and stroke ordering from simple user input. And then, we decompose whole character shape into some strokes by using polygonal approximation technique. Next, the stroke animation for each stroke is automatically generated by the scan line algorithm ordered by the stroke direction. Finally, the ordered scan lines are compressed into some integers by reducing coordinate redundancy As a result, the stroke animation of our system is even smaller than GIF animation. Our method can be extended to rendering and animation of Hangul or general 2D shape based on vector graphics. We have the plan to find the method to automate the stroke segmentation and ordering without user input.

• The Journal of the Korea Contents Association
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• v.10 no.10
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• pp.54-67
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• 2010

Applying the conventional machine-learning method which has been frequently used in health-care area has several fundamental problems for modern U-health service analysis. First of all, we are still lack of application examples of the traditional method for our modern U-health environment because of its short term history of U-health study. Second, it is difficult to apply the machine-learning method to our U-health service environment which requires real-time management of disease because the method spends a lot of time in the process of learning. Third, we cannot implement a personalized U-health diagnosis system using the conventional method because there is no way to assign weights on the disease-related variables although various kinds of machine-learning schemes have been proposed. In this paper, a novel diagnosis scheme PCADP is proposed to overcome the problems mentioned above. PCADP scheme is a personalized diagnosis method and it makes the bio-data analysis just a 'process' in the U-health service system. In addition, we offer a semantics modeling of the U-health ontology framework in order to describe U-health data and service specifications as meaningful representations based on this PCADP. The PCADP scheme is a kind of statistical diagnosis method which has characteristics of flexible structure, real-time processing, continuous improvement, and easy monitoring of decision process. Upto the best of authors' knowledge, the PCADP scheme and ontology framework proposed in this paper reveals one of the best characteristics of flexible structure, real-time processing, continuous improvement, and easy monitoring among recently developed U-health schemes.

• Journal of Science and Technology Studies
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• v.12 no.2
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• pp.159-183
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• 2012

In order to create a desirable science culture needed in our society, it is necessary to overcome the foreignness of science and technology and to overcome severance from tradition. In this context, this article attempts to understand the characteristics of our traditional science and to explore the possibility of forming a desirable science culture through astronomy, which is an example of traditional science. Thus, this article examined the general characteristics of astronomy that had appeared first in ancient civilization. It also focused on the fact that each civilization has its own unique cultural elements together with astronomical knowledge as a field of science in traditional astronomy. Calendar and lifa(曆法), which are considered science of time, are closely connected with people's daily lives and reveal cultural differences clearly among the subfields of astronomy. In all ancient civilizations, time was represented based on the movements of the sun and the moon, but how time should be concretely represented varied, depending on different cultures. As a result, various calendar system emerged. Throughout East Asia, including our country, the luni-solar calendar was used. The calendar in East Asia, unlike that in the West, was the one derived from the lifa, which was very complex and elaborate astronomical work. The characteristics of the luni-solar calendar can be clearly found in the seasonal customs that represent people's daily lives well; however, lots of so-called superstition are also included in the seasonal customs. For this reason, it is easy to misunderstand that our calendar system is unscientific, or to suspect that our overall traditional science lacks scientific aspects. However, proper understanding of the calendar and the lifa of East Asia can confirm that scientific aspects certainly existed in our tradition. This will be the vital link to tradition that will help overcome the foreignness of today's science and technology.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.31 no.4
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• pp.31-41
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• 2018

Objectives : The purpose of this study is to establish the basis of the assessment tools that can be used for the diagnosis and assessment of skin diseases by reviewing the present status of the assessment tools used in domestic and overseas traditional medicine papers for the past five years. Methods : This study is based on analysis of papers on clinical trials about atopic dermatitis, psoriasis, acne, urticaria published from 1st July 2013 to 30th June 2018. The papers were searched from Oriental Medicine Advanced Searching Integrated System(OASIS), Korean Traditional Knowledge Portal(KTKP), Pubmed, MEDLINE. Results : 40 articles on atopic dermatitis, 34 articles on psoriasis, 26 articles on acne and 10 articles on urticaria were selected. Among them, 38 articles(90%), 20 articles(85%), 22 articles(85%) and 5 articles(50%) used assessment tools in order. Conclusions : SCoring Atopic Dermatitis(SCORAD), objective SCORing of Atopic Dermatitis index(OSI), Eczema Area and Severity Index(EASI) were used in studies on atopic dermatitis. In the case of psoriasis, The Psoriasis Area and Severity Index(PASI) was used in most papers. In the study on acne, most of Korean papers used Korean Acne Grading System(KAGS), but in the overseas papers, various assessment tools were used. In the case of urticaria, the rate of use of the assessment tool was the lowest, but the assessment tool used was unified by Urticaria Activity Score(UAS). Since skin diseases can cause stress and a decrease in quality of life, the quality of life should be taken into account when evaluating treatment effects.

• 대한공업교육학회지
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• v.31 no.1
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• pp.146-169
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• 2006

'VEC 2010' is a mid-long term scheme on the development of Vocational Education for Chung-buk which is to be accomplished by 2010. On the basis of this scheme, schools adopt 3-Type curriculum considering their conditions and covert themselves into specialized high school which is associated with local industry cluster. They also establish combined high schools which run various curriculums emphasizing basic vocational abilities so that they can meet the needs of demanders The activation plan of Chung-buk province vocational education is a dualized one which is for both ongoing education for entrance into a school of higher grade and the last education for employment, which is supposed to foster the human resources required by the knowledge-based society in the 21st century. The specific contents of 'VEC 2010' is as follows. First, VEC 2010 presents 2 kinds of mid-long term management system for industrial high schools: general industrial high school, specialized high school(single type, combined type). Second, VEC 2010 arranges and run the 3-Type of curriculum of Chung-buk province industrial high schools: employment-centered type, entering higher school-centered type, inaugurating enterprise-centered type. Third, VEC 2010 divides Chung-buk province industrial high schools into 4 districts and suggests mid-long term reorganization plan of system. Fourth, VEC 2010 suggests the substantial strategies of Chung-buk province vocational education: scholarship improvement in the vocational investigation course of the government-sponsored scholastic aptitude test, education for improving basic vocational abilities, substantial management of joint laboratory, administrative and financial support of development of specialized, licensed textbooks. reenforcement of career education.