• 한국미생물·생명공학회지
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• 제17권5호
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• pp.489-493
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• 1989

쥐 하이브리도마 Alps 25-3를 이용하여 무혈청 배지 KM3의 각 성분이 세포증식과 항체생성에 미치는 영향을 조사하여 무혈청 배지를 최소화한 KMS를 결정하였다. Alps 25-3에 영향을 미치는 것으로 조사된 transferrin, ethanolamine 그리고 BSA만을 혼합하여 결정한 KMS를 다른 쥐 하이브리도마인 A4W, KW 그리고 HCGK에 적용시켰다. KM5를 KM3와 비교하였을 때 세포증식과 항체생산이 거의 동일한 양상을 보였다. 그러나 혈청배지와 비교하여 보면 최고 세포농도는 50-90%, 최종 단일클론항체의 농도는 85-100%의 수준을 보였다. 본 연구의 결과로 쥐 하이브리도마 세포를 무혈청 배지에서 배양시킬 때 필수적으로 첨가기어야 하는 성분들이 조사되었으므로 대량배양에 응용하거나 대사를 연구하는데 유용하게 이용되어지리라 본다.

• 한국콘텐츠학회논문지
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• 제8권3호
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• pp.67-74
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• 2008

게임, 애니메이션, 영화와 같은 다양한 영상 미디어가 발전함에 따라 한 가지의 콘텐츠 소스를 여러 형태로 이용을 하는 확장으로서 피규어(Figure)라 일컬어지는 상품이 만들어 졌으며, 오늘날 많은 인기를 모으고 있다. 하지만 국내에서는 어린이들의 단순한 장난감 취급을 받으며 관련 산업의 발전이 더디게 이루어지고 있는 반면 외국의 경우 콘텐츠 산업에 있어서 마케팅의 수단으로 중요성을 인정받고 있다. 본 논문에서는 피규어에 대한 의미와 종류를 알아보고 철학적 사상과 이론을 통하여 피규어가 지닌 성격을 학문적고찰(발터 벤야민, 프로이드 및 심리학자들의 이론)을 통하여 분석하였다. 현재 국내 외 피규어 산업은 발전을 지속하고 있으며, 특히 한류 스타라는 콘텐츠를 활용한 피규어는 문화 아이템으로서 많은 가치를 지니고 있으며 발전성이 있음을 알 수 있다.

• 한국태양에너지학회 논문집
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• 제30권1호
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• pp.34-41
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• 2010

Gas hydrates are solid solutions when water molecules are linked through hydrogen bonding and create host lattice cavities that can enclose many kinds of guest(gas) molecules. There are plenty of methane(gas) hydrate in the earth and distributed widely at offshore and permafrost. Several schemes, to produce methane hydrates, have been studied. In this study, depressurization method has been utilized for the numerical model due to it's simplicity and effectiveness. IMPES method has been used for numerical analysis to get the saturation and velocity profile of each phase and pressure profile, velocity of dissociation front progress and the quantity of produced gas. The values calculated for the sample length of 10m, show that methane hydrates has been dissolved completely in approximately 223 minutes and the velocity of dissociation front progress is 3.95㎝ per minute. The volume ratio of the produced gas in the porous media is found to be about 50%. Analysing the saturation profile and the velocity profile from the numerical results, the permeability of each phase in porous media is considered to be the most important factor in the two phase flow propagation. Consequently, permeability strongly influences the productivity of gas in porous media for methane hydrates.

• 한국통신학회논문지
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• 제35권11A호
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• pp.1085-1090
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• 2010

Ultra wideband (UWB) technologies have been developed to exploit a new spectrum resource in substances and to realize ultra-high-speed communication, high precision geolocation, and other applications. The energy of UWB signal is extremely spread from near DC to a few GHz. This means that the interference between conventional narrowband systems and UWB systems is inevitable. However, the interference effects had not previously been studied from UWB wireless systems to conventional mobile wireless systems sharing the frequency bands such as Cellular CDMA and Korean PCS. This paper experimentally evaluates the interference from two kinds of UWB sources, namely a direct-sequence spread-spectrum UWB source and an impulse radio UWB source, to a Cellular CDMA and K-PCS digital transmission system. The average frame error rate degradation of each system arc presented. From these experimental results, we show that in all practical cases UWB system can coexist with Cellular CDMA and K-PCS terminal without causing any dangerous interference.

• 고전문학과교육
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• 제16호
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• pp.65-90
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• 2008

This study is on the reading method of Korean old poetry in the digital age. Now days, we are living in digital media. The method of getting, organizing, and producing information is changed a lot. So for keeping pace with this change, we should reflect our teaching method. Of course, the purpose of our reflection is not to follow digital media's method and change or alter existing reading or teaching method, but it is to identify proper own reading strategy of old poetry and adjust existing method for our student who are skilled with digital media. We propose three kinds of method for student readers in school. These are 1) starting like a newspaper reader, 2) getting context (reading, making hyperlink), and 3) figuring as an architect (reading as space or scenery creator). In addition, we think about recent tendency that Korean old poetry is de-constructed and re-constructed as digital contents in digital space. We call it digitalizing of old poetry. The experiencing old poetry as a digital contents is different from reading old poetry, so we cannot alter old poetry reading experience into experience of digital contents. In conclusion, digitalizing contents should be used restrictively to fertilize reading experience of original old poetry.

• Journal of information and communication convergence engineering
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• 제18권3호
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• pp.188-193
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• 2020

In this paper, we examine whether the 3D image experience through a light-field display device showed the difference in the arousal of the user compared with the 2D image experience. For our experiment, the Looking Glass^TM (LG) was used as a lightfield display device that provided 3D images, and 2D images were provided by digital and printed images. The subject's facial behavior during each media experience was recorded for analysis and the degree of arousal was measured by FaceReader^TM. As a result, the first image presented in the first order among the three kinds of images showed that there was a statistical difference in the degree of arousal between the three media. However, no significant differences were found between the three media in the other images. This may be because the arousal did not increase from the experience of the second image through the LG, owing to habituation. In conclusion, the 3D imaging experience may appear in the beginning, but does not continue.

• ETRI Journal
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• 제37권1호
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• pp.88-96
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• 2015

The purpose of this study is to design an authoring tool and a corresponding device for an olfactory display that can augment the immersion and reality in broadcasting services. The developed authoring tool allows an olfactory display to be properly synchronized with the existing video service by applying the standardized format using ISO/IEC 23005 (MPEG-V) and the corresponding developed scent display device. To propose the proper data format for the olfactory display, we have analyzed both the multimodal combination and the cross-modality related to the olfactory display. From the results of the analysis, we derived a set of olfactory parameters for the olfactory display that are related to emotion. The analyzed parameters related to emotion in an olfactory display are synchronization, scent intensity, scent persistence, and hedonic tone. These parameters should be controlled so that the olfactory display can be in harmony with the existing media to augment emotion. In addition, we developed a scent display device that can generate many kinds of scents and that satisfies design conditions for olfactory parameters that are for use with broadcasting services.

• Journal of Nutrition and Health
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• 제29권2호
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• pp.159-165
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• 1996

This study was performed to investigate the effects of concentration and degree of unsaturation of fatty acids on cellular proliferation and lipid peroxide production, using primary skin fibroblasts from neonate rats Fibroblasts (CPD : 2.8-5.4). Cells were cultured either in control medium (Dulbecco's modified Eagle's medium supplement with 10% fetal bovine serum) or in media supplemented with various kinds (stearic, oleic, linoleic, arachidonic, linolenic, eicosapentaenoic acid) and amounts (5, 10, 25, 50, 100, 150uM)of fatty acids. Cellular proliferation ratio and lipid peroxice production were measured and morphological changes were observed. Cellular proliferation was inhibited and morphological changes were observed. Cellular proliferation was inhibited and morphological changes were observed in cells grown in stearic containing media. Oleic, arachidonic, and eicosapentaenoic aicd tend to stimulate cellualar proliferation, and linolenic acid had no effects. Lipid peroxide concentrations in fibroblasts increased in proportion to the contents and unsaturation of fatty acids in media. Especially supplementation of arachidonic acid accelerated cellualr lipid peroxidation. Free radicals may cause severs damage to biological molecules, so lipid peroxidation probably contributes cellular membrane damages. However there were little relationship between lipid peroxide production and cellular proliferation in this study. (Korean J Nutrition 29(2) : 159~165, 1996)

• 한국입자에어로졸학회지
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• 제8권2호
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• pp.83-88
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• 2012

The electrical wire explosion process in liquid media is promising for nano-sized metal and/or alloy particles. The hybrid Pt/Fe-Cr-Al and Pt/Ni-Cr-Fe nanoparticles for exhaust emission control system are synthesized by electrical wire explosion process in liquid media. The alloy powders have spherical shape and nanometer size. According to the wire component, while Pt/Fe-Cr-Al nanoparticles are shown the well dispersed Pt on the Fe-Cr-Al core particle, Pt/Ni-Cr-Fe nanoparticles are shown the partially separated Pt on the Ni-Cr-Fe core particle. Morphologies and component of two kinds of hybrid nano catalyst particles were characterized by transmission electron microscope and energy dispersive X-ray spectroscopy analysis.

• Journal of Plant Biology
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• 제19권2호
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• pp.45-48
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• 1976

Anthers of cultivated Paeonia albiflora were cultured on media supplemented with various combinations of growth regulators. Although the number of anthers with emerged calluses were very few, in the sectioned anthers were found many multinucleate, 2-celled, or multicellular microspores, the one-celled multinucleate microspores being most abundant in number, and the multicellular ones the least. In 2-celled or 3-celled microspores two kinds were observed: one is ordinary one with single nucleus in each cell, and the other is multinucleate one. Majority of the 2-celled microspores was found to be of equational-division irrespective of whether they were multinucleate micropores or ordinary nonmultinucleate ones.