Journal of Nutrition and Health

  • 제29권2호
  • /
  • Pages.159-165
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  • 1996
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  • 2288-3886(pISSN)
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  • 2288-3959(eISSN)
한국영양학회 (The Korean Nutrition Society)
권호 표지

신생흰쥐 피부섬유아세포의 배양액의 지방산의 종류와 양을 변화시켰을 때 세포의 증식과 지질과산화물 생성에 미치는 영향

The Effects of Fatty Acids Supplementation in Culture Medium on Proliferation and Lipid Peroxides Production of Fibroblast from Neonate Rats

  • 장영애 (이화여자대학교 식품영양학과)
  • 발행 : 1996.03.01
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초록

This study was performed to investigate the effects of concentration and degree of unsaturation of fatty acids on cellular proliferation and lipid peroxide production, using primary skin fibroblasts from neonate rats Fibroblasts (CPD : 2.8-5.4). Cells were cultured either in control medium (Dulbecco's modified Eagle's medium supplement with 10% fetal bovine serum) or in media supplemented with various kinds (stearic, oleic, linoleic, arachidonic, linolenic, eicosapentaenoic acid) and amounts (5, 10, 25, 50, 100, 150uM)of fatty acids. Cellular proliferation ratio and lipid peroxice production were measured and morphological changes were observed. Cellular proliferation was inhibited and morphological changes were observed. Cellular proliferation was inhibited and morphological changes were observed in cells grown in stearic containing media. Oleic, arachidonic, and eicosapentaenoic aicd tend to stimulate cellualar proliferation, and linolenic acid had no effects. Lipid peroxide concentrations in fibroblasts increased in proportion to the contents and unsaturation of fatty acids in media. Especially supplementation of arachidonic acid accelerated cellualr lipid peroxidation. Free radicals may cause severs damage to biological molecules, so lipid peroxidation probably contributes cellular membrane damages. However there were little relationship between lipid peroxide production and cellular proliferation in this study. (Korean J Nutrition 29(2) : 159~165, 1996)

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참고문헌

  1. 노화 3장 노화이론 김숙희;김화영
  2. J Nutr v.51 The influence of dietary fat and carbohydrate on growth and longevity in rats French,G.R.;Ingram,R.H.;Uram,A.J.;Barrow,G.P.;Surf,R.W.
  3. Rev Biol Res Aging v.4 Food restriction research : past and present status Yu,B.P.
  4. J Gerontol v.26 no.4 Free radical theory of aging : Effect of the amount and degree of unsaturation of dietary fat on mortality rate Harman,D.
  5. J Nutr v.125 no.3S Overview : Dietary approaches for reducing cardiovascular risks Dwyer,J.
  6. Atherosclerosis v.67 On the association of body cholesterol pool size with age, HDL cholesterol and plasma total cholesterol concentration in humans Normen,E.M.
  7. Exp Mol Pathol v.18 Essential fatty acid requirements of cells in tissue culture : A review Bailey,J.M.;Dunbar,L.M.
  8. Lipids v.15 no.6 Utilzation of polyunsaturated fatty acids by human diploid cells aging in vitro Lynch,R.D.
  9. Gastroenterol v.95 Role of activation of protein kinase C in the stimulation of colonic epithelial proliferation by unsaturated fatty acids Craven,P.A.;DeRubertis,F.R.
  10. J Cell Physiol v.120 Rat mammary preadipocytes in culture produce a trophic agent for mammary epithlia : prostaglandin E₂ Rudland,P.S.;Davies,A.C.T.;Taso,S.W.
  11. Am J Clin Nutr v.53 Membrane damage due to lipid peroxidation Niki,E.;Yamamoto,Y.;Komura,E.;Sato,K.
  12. Ann NY Acad Sci USA v.663 Lipid peroxidation and hepatocyte death investigation of a possible mechanism of oxidative cell injury Carini,R.;Parola,M.;Dianzani,M.U.;Albano,E.
  13. Free Radical Res Comm v.1 Repair of DNA damage induced by reactive oxygen species Breimer,L.
  14. Chichester DNA and free radicals Halliwell,B.;Aruoma,O.I.(eds.)
  15. Nutr Rev no.8 Free radicals and antioxidants : A personal view Halliwell,B.
  16. Lipids v.27 no.10 Effects of dietary linseed oil and marine oil on lipid peroxidation in monkey liver in vivo and in vitro Kaasgaard,S.G.;Holmer,G.;Hoy,C.E.;Behrens,W.A.;BeareRogers,J.L.
  17. Ann NY Acad Sci v.663 Cell proliferation and cell death in immunosenescence Monti D.;Troland L.;Grassilli,E.;Agnesini,C.;Tropea,F.;Babieri,D.;Capri,M.;Cristofalo,E.A.;Salvioli,S.;Ronchetti,I.;Bellomo,G.;Cossarizza,A.;Franceschi,C.
  18. Assay for serum lipid peroxide level and its clinical significance. In : Yagi K ed. Lipid peroxides in biology and medicine Yagi,K.
  19. Nutr Rev v.7 Lipofuscin, the age pigment Wolf,G.
  20. 한국영양학회지 v.29 no.1 배양액내 지방함량의 변화가 신생흰쥐 피부섬유아세포의 노화와 지질과산화물 생성에 미치는 영향 장영애;김화영
  21. Animal cell culture Aging of cultured Human skin fibroblasts. Harley,C.B.;Pollard JW(ed.);Walker JM(ed.)
  22. Animal Cell culture : Apractical approach Scaling-up of animal cell cultures Griffiths,B.Freshney,RI(ed.)
  23. Assay for blood plasma or serum. In : Methods in enzymology v.105 Yagi,K.
  24. J Biol Chem v.262 Linoleate metabolites enhanc the in vitro proliferative response of mouse mammary epithelial cells to epidermal growth factor Bandyopadhyay,G.K.;Imagawa,W.;Wallace,D.;Nandi,S.
  25. Proc Natl Acad Sci USA v.86 Phospholipids containing polyunsaturated fatty acyl groups are mitogenic for normal mouse mammary epithelial cells in serum-free primary cell culture Imagawa,W.;Bandyopadhyay,G.K.;Wallace,D.;Nandi,S.
  26. Proc Natl Acad Sci USA v.78 Rapid clonal growth and serial passage of human diploid fibroblasts in a lipid-enriched synthetic medium supplemented with epidermal growth factor, insulin and dexamethasone Bettger W.J.;Boyce S.T.;Walthall B.J.;Ham R.G.
  27. Can J Biochem v.57 Multiple effects of serum low density lipoprotein on cultured human fibroblasts Spain,M.J.;Wosu,L.O.;Kalant,N.
  28. J Lipid Res v.20 Modification of the fatty acid composition of cultured human fibroblasts Spector,A.A.;Kiser,R.E.;Denning,G.M.;Koh,S.W.M.;DeBault,L.E.
  29. Prostaglandins v.33 The effect of PGE₂ on the activation of quiescent lung fibroblasts Fine,A.;Goldstein,R.H.
  30. Biochem Biophysics Res Comm v.169 no.3 Growth inhibition of human fibroblasts by epidermal growth factors in the presence of arachidonic acid Hori,T.;Yamanaka,Y.;Hayakawa,M.;Shibamoto,S.;Oku,N.;Ito,F.
  31. J Nutr v.120 Modulation of mouse mammary tumor growth and linoleate enhanced metastasis by oleate Buckmann,D.K.;Chapkin,R.S.;Erickson,K.L.
  32. Kor J Nutr v.27 no.9 Effect of individual fatty acids on synthesis and secretion of apolipoprotein and lipoprotein in Hep-G2 cells Choue,R.W.;Cho,B.H.
  33. Lipids v.20 Fatty acid metabolism and cell proliferation. ⅤⅡ. Antioxidant effects of tocopherols and their quinones Lindsey,J.A.;Zhang,H.;Kaseki,H.;Morisaki,N.;Sato,T.;Cornwell,D.G.
  34. J Gerontol v.33 no.2 Body weight increment and length of life : The effect of genetic constitution and dietary protein Goodlich,C.L.
  35. Proc Natl Acad Sci USA v.82 Peroxide-producing potential of tissues : Inverse correlation with longevity of mammalian species Culter,R.G.
  36. Am J Clin Nutr v.62 Antioxidants and immune response in aged persons : overview of present evidence Meydani,S.N.;Wu,D.;Santos,M.S.;Hayek,M.G.
  37. Ann NY Acadd Sci v.663 Oxidants are major contributor to aging Ames,B.N.;Shigenaga,M.K.
  38. Gerontol v.25 Oxidative stress as a causal factor in differentiation and aging : A unifying hypothesis. Exp Sohal,R.S.;Allen,R.G.