Initial Divisions of Microspores in In Vitro Cultured Anthers of Cultivated Paeonia albiflora

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Anthers of cultivated Paeonia albiflora were cultured on media supplemented with various combinations of growth regulators. Although the number of anthers with emerged calluses were very few, in the sectioned anthers were found many multinucleate, 2-celled, or multicellular microspores, the one-celled multinucleate microspores being most abundant in number, and the multicellular ones the least. In 2-celled or 3-celled microspores two kinds were observed: one is ordinary one with single nucleus in each cell, and the other is multinucleate one. Majority of the 2-celled microspores was found to be of equational-division irrespective of whether they were multinucleate micropores or ordinary nonmultinucleate ones.