• 제목/요약/키워드: key elements

검색결과 1,343건 처리시간 0.024초

Optimization of Medium Composition for Lipopeptide Production from Bacillus subtilis N7 using Response Surface Methodology

  • Luo, Yi;Zhang, Guoyi;Zhu, Zhen;Wang, Xiaohui;Ran, Wei;Shen, Qirong
    • 한국미생물·생명공학회지
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    • 제41권1호
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    • pp.52-59
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    • 2013
  • The nutritional requirements for the maximum production of lipopeptides by Bacillus subtilis N7 (B. subtilis N7) were investigated and optimized using response surface methodology (RSM) under shake flask fermentation. A one-factor-at-a-time experimental setup was used to screen carbon and nitrogen sources. A Plackett-Burman design (PBD) was employed to screen the most critical variables for lipopeptides production amongst ten nutritional elements. The central composite experimental design (CCD) was finally adopted to elucidate the composition of the fermentation medium. Statistical analyses (analysis of variance, ANOVA) of the results showed that KCl, $MnSO_4$ and $FeSO_4{\cdot}6H_2O$ were important components and that their interactions were strong. Lipopeptide production was predicted to reach 709.87 mg/L after a 60 h incubation using an optimum fermentation medium composed of glucose 7.5 g/L, peanut oil 1.25 g/L, $MgSO_4$ 0.37 g/L, $KH_2PO_4$ 0.75 g/L, monosodium glutamate 6.75 g/L, yeast extract and $NH_4Cl$ (5:3 w/w) 10 g/L, KCl 0.16 g/L, $FeSO_4{\cdot}6H_2O$ 0.24 mg/L, $MnSO_4$ 0.76 mg/L, and an initial pH of 7.0. Lipopeptide production ($706.57{\pm}3.70$ mg/L) in the optimized medium confirmed the validity of the predicted model.

Experimental study on the shear failure model for concrete under compression-shear loading

  • Shu, Xiaojuan;Luo, Yili;Zhao, Chao;Dai, Zhicheng;Zhong, Xingu;Zhang, Tianyu
    • Computers and Concrete
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    • 제29권2호
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    • pp.81-92
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    • 2022
  • The influence of normal stress perpendicular to the potential shear plane was always neglected in existing researches, which may lead to a serious deviation of the shear strength of concrete members in practice designs and numerical analyses. In this study, a series of experimental studies are carried out in this paper, which serves to investigate the shear behavior of concrete under compression shear loading. Based on the test results, a three-phase shear failure model for cohesive elements are developed, which is able to take into consideration the influence of normal stress on the shear strength of concrete. To identify the accuracy and applicability of the proposed model, numerical models of a double-noted concrete plate are developed and compared with experimental results. Results show that the proposed constitutive model is able to take into consideration the influence of normal stress on the shear strength of concrete materials, and is effective and accurate for describing the complex fracture of concrete, especially the failure modes under compression shear loadings.

A Systematic Process of Product Design Based on Customer Preferences

  • Chun Young H.;Baek Ingie;Jung Eui S.
    • 한국품질경영학회:학술대회논문집
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    • 한국품질경영학회 1998년도 The 12th Asia Quality Management Symposium* Total Quality Management for Restoring Competitiveness
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    • pp.325-332
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    • 1998
  • In the context of total quality management, customer satisfaction is a key factor of success. Customer needs have been in the past described with rather vague words. In order to lead in the competitive market, product designers must be willing to interpret and reflect customer perceptions of a product on the design. The objective of this research is to develop a systematic process capable of linking customer preferences on a product to the design of product elements or specifications. The design process consists of multivariate statistical analyses, semantic differentials, and multidimensional scaling techniques under the framework of a methodology known as quality function deployment which is frequently used to construct a quality design process. The process being established is expected to serve as an effective means to communicate between the customer and the designer through proper representational schemes of design elements.

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비디오 컨텐츠 검색을 위한 형태론적 손짓 인식 알고리즘 (Morphological Hand-Gesture Algorithm for Video Content Navigation)

  • 김정훈;최종호;최종수
    • 대한전자공학회:학술대회논문집
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    • 대한전자공학회 2001년도 하계종합학술대회 논문집(4)
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    • pp.37-40
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    • 2001
  • The most important issues in gesture recognition are the simplification of algorithm and the reduction of processing time. The mathematical morphology based on geometrical set theory is best used to perform the real-time processing. A key idea of the algorithm proposed in this paper is to apply morphological shape decomposition. The primitive elements extracted from a hand gesture have very important information including the directivity of the hand gestures. Based on this algorithm, we proposed the morphological hand-gesture recognition algorithm using feature vectors extracted from lines connecting the center points of a main-primitive element and sub-primitive elements. Through the experiments, we applied to the video contents browsing system with natural interactions and demonstrated the efficiency of this algorithm.

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산업기술개발 수요조사사업에 대한 사례 연구

  • 이재하
    • 기술경영경제학회:학술대회논문집
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    • 기술경영경제학회 1997년도 제12회 동계학술발표회 논문집
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    • pp.89-103
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    • 1997
  • A nation's technology policy is based on the broad principles that govern the allocation of its technological resources. But the cost of inadequate investments in technology development is particularly high in today's global1y-competitive environment where continued technological advancements are critical to sustain. This paper presents a methodology for searching the new and /or advanced technologies to promote Korea's technological and economic competitiveness by industrial technology policy. The conceptual framework of industrial technology development, which is the main focus of this Paper, was designed using both Top-down and Bottom-up approach And the technology classification system was modified reviewing the previous it's system. Supporting the technology input are three key technology elements; proprietary technology, generic technology, infratechnology Competitive survival in any advanced economy will depend upon technology-based strategies that emphasize the integration of these elements of industrial technology.

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소비자의 선호도에 근거한 체계적 제품설계 절차 (A Systematic Process of Product Design Based on Cutomer Preferences)

  • 전영호;백인기;정의승
    • 품질경영학회지
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    • 제27권3호
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    • pp.142-153
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    • 1999
  • In the context of total quality management, customer satisfaction is a key factor of success. Customer needs have been in the past described with rather vague words. In order to lead in the competitive market, product designers must be willing to interpret and reflect customer perceptions of a product on the design. The objective of this research is to develop a systematic process capable of linking customer preferences on a product to the design of product elements or specifications. The design process consists of multivariate statistical analyses, semantic differentials, and multidimensional scaling techniques under the framework of a methodology known as quality function deployment which is frequently used to construct a quality design process. The process being established is expected to serve as an effective means to communicate between the customer and the designer through proper representational schemes of design elements.

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준천정 위성 궤도 특성 및 항법정보 연구 (Study of Quasi Zenith Satellite Orbit and Navigation Messages)

  • 김정래
    • 한국항공운항학회지
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    • 제15권1호
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    • pp.11-17
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    • 2007
  • One of the key elements for developing GNSS (Global Navigation Satellite Systems) is the comprehensive analysis of GNSS satellite orbit including the capabilities to generate precision navigation message. The orbit characteristics of Japan's own GNSS system, called QZSS (Quasi Zenith Satellite System) is analyzed and its navigation message, which includes orbit elements and correction terms, is investigated. QZSS-type orbit simulations were performed using a precision orbit integrator in order to analyze the effect of perturbation forces, e.g. gravity, Moon, Sun, etc., on the orbit variation. A preliminary algorithm for creating orbit element corrections was developed and its accuracy is evaluated with the simulation data.

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한국어 음성합성기의 성능 향상을 위한 합성 단위의 유무성음 분리 (Separation of Voiced Sounds and Unvoiced Sounds for Corpus-based Korean Text-To-Speech)

  • 홍문기;신지영;강선미
    • 음성과학
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    • 제10권2호
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    • pp.7-25
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    • 2003
  • Predicting the right prosodic elements is a key factor in improving the quality of synthesized speech. Prosodic elements include break, pitch, duration and loudness. Pitch, which is realized by Fundamental Frequency (F0), is the most important element relating to the quality of the synthesized speech. However, the previous method for predicting the F0 appears to reveal some problems. If voiced and unvoiced sounds are not correctly classified, it results in wrong prediction of pitch, wrong unit of triphone in synthesizing the voiced and unvoiced sounds, and the sound of click or vibration. This kind of feature is usual in the case of the transformation from the voiced sound to the unvoiced sound or from the unvoiced sound to the voiced sound. Such problem is not resolved by the method of grammar, and it much influences the synthesized sound. Therefore, to steadily acquire the correct value of pitch, in this paper we propose a new model for predicting and classifying the voiced and unvoiced sounds using the CART tool.

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영국 전통 패션브랜드 혁신에 관한 연구 (A Study on the Renovations of the British Traditional Fashion Brands)

  • 이해연;김문영;은영자
    • 한국의상디자인학회지
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    • 제6권3호
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    • pp.97-109
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    • 2004
  • As lifestyle changes, consumer behavior becomes emotional. And fashion brands are responding the behavior. The brands reflect changes of the needs and the values of consumers. This study analyzed the renovation strategies of the British traditional fashion brands through a literature survey. The common renovation strategies of the British traditional fashion brands were the renovations in the design which harmonized traditional elements with modem elements which is the key, and the renovations in the promotion and the distribution of commodities. The most effective renovation strategy is to combine the three renovations.

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Process Optimization for High Frequency Performance of InP-Based Heterojunction Bipolar Transistors

  • Song, Yongjoo;Jeong, Yongsik;Yang, Kyounghoon
    • JSTS:Journal of Semiconductor Technology and Science
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    • 제3권1호
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    • pp.33-41
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    • 2003
  • In this work, process optimization techniques for high frequency performance of HBTs are presented. The techniques are focused on reducing parasitic base resistance and base-collector capacitance, which are key elements determining the high frequency characteristics of HBTs. Several fabrication techniques, which can significantly reduce the parasitic elements of the HBTs for improved high frequency performance, are proposed and verified by the measured data of the fabricated devices.