• Title/Summary/Keyword: karyotype formula

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Karyotype Analysis of Eight Korean Native Species in the Genus Iris

  • Kim, Hyun-Hee;Park, Young-Wook;Yoon, Pyung-Sub;Choi, Hae-Woon;Bang, Jae-Wook
    • Korean Journal of Medicinal Crop Science
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    • v.12 no.5
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    • pp.401-405
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    • 2004
  • Karyotypes were established in the eight Korean native species of the genus Iris. Chromosome numbers were 2n=50 in I. koreana and 2n=42 in I. uniflora var. carinata and their karyotype formulas were K = 2n = 50 = 14m + 28sm + 8st and K = 2n = 42 = 16m + 26sm, respectively. I. dichotoma and I. pseudoacorus were diploids of 2n=34. However, they showed different karyotype formulas: K = 2n = 34 = 26m + 6sm + 2st in I. dichotoma and K = 2n = 34 = 8m + 24sm + 2st in I. pseudoacorus. I. setosa, and I. pallasii var. chinensis carried the same chromosome numbers of 2n=40, but they showed different patterns of karyotype formula: K = 2n = 40 = 22m + 14sm + 4st in I. setosa and K = 2n = 40 = 26m + 12sm + 2st in I. pallasii var. chinensis. I. sanguinea was a diploid of 2n=28 and the karyotype formula was K = 2n = 28 = 14m + 14sm. I. ensata var. spontanea was a diploid of 2n=24 and the karyotype formula was K = 2n = 24 = 10m + 14sm. Each species showed characteristic chromosome composition with a pair of satellite chromosome except I. koreana with three pairs of satellite chromosomes. The chromosomes of I. dichotoma and I. uniflora were comparatively short, while the chromosomes of I. ensata were remarkably bigger than those of other species. These cytological data will give a useful information for the identification and breeding program of the Iris plants.

Karyotype of Dwarf Loach, Kichulchoia brevifasciata (Pisces: Cobitidae) from Korea (미꾸리과 소형어류 좀수수치, Kichulchoia brevifasciata의 핵형)

  • Kim, Ik-Soo;Kim, Eun-Jin
    • Korean Journal of Ichthyology
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    • v.20 no.1
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    • pp.61-65
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    • 2008
  • The karyotype analysis of Kichulchoia brevifasciata was carried out, known as an end-angered small endemic cobitid fish of Korea. Five females and one male were collected from Geum-san-myeon, Goheung-gun, Jeollanam-do, Korea and its gill, kidney and digestive tract tissues were treated by flame-drying technique. The karyotype was found to be 2n= 48 from 33 cells; 16 meta-submetacentric and 32 subtelo-telocentric chromosomes; 64 FN (fundamental number). This karyotype showed a remarkably different karyotype formula from Niwaella multifasciata which is closely related to this species.

Cytogenetic Study of Pleuronectes obscurus, Konosirus punctatus and Pseudoblennius percoides

  • Kim, Eun-Mi;An, Hye-Sook;Park, In-Seok
    • Fisheries and Aquatic Sciences
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    • v.10 no.1
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    • pp.24-29
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    • 2007
  • Our objective was to clarify the cytogenetic characteristics, including karyotypes, cellular DNA content, and nuclear size of erythrocytes, of black plaice Pleuronectes obscurus, dotted gizzard shad Konosirus punctatus, and perch sculpin Pseudoblennius percoides, collected from the coastal areas of Jo Island, Busan, Korea. Karyotypes of P. obscurus and K. punctatus both had a diploid number of 48 and a fundamental number (FN) of 48, with a chromosome formula of 48T. The karyotype of p. percoides had a diploid number of 46 and FN of 56, with a chromosome formula of 10SM +36T. No sex-associated heteromorphic pairs were detected for any species. The variation in DNA values (P. obscurus=1.15 pg/nucleus, K. punctatus=1.56pg/nucleus, P. percoides=1.11 pg/nucleus) was positively related to variation in chromosome FN.

Cytological Studies of the Paeonia Species Grown Wild or Cultivated in Korea I Karyotypes of cultivated P. albiflora varieties (한국에 자생 또는 재배되는 Paeonia속의 세포학적 연구 재배균약 Paeonia albiflora의 핵형)

  • 한창열
    • Journal of Plant Biology
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    • v.19 no.2
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    • pp.33-36
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    • 1976
  • Present work was attempted to investigate the karyotype, the reciprocal translocation of chromosomes and the geographical distribution of three Paeonia species grown wild in Korea with the aim of elucidating the taxonomical affinities of the species. Prior to conducting these works, it was required to clarify the karyotypes of the cultivated varieties of P. albiflora which has been grown since long time ago in this coundtry for medicinal value. Present report is part of the series of karyotype studies now being extensively conducted using varieties (or lines) of P. albiflora collected across the country. Arm ratio, number of trabant, kind of trabant chromosomes and karyotype formula are different among the six cultivated varieties. Excepting one, five varieties used had invariably trabants on D and E chromosomes while no trabant was found in C chromosome.

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Karyotypic Analysis and Physical Mapping of rRNA Gene Loci in Persicaria tinctoria (쪽의 핵형분석과 rRNA 유전자의 염색체상 위치)

  • Choi, Hae-Woon;Lee, Sang-Hoon;Kim, Soo-Young;Bang, Jae-Wook
    • Korean Journal of Medicinal Crop Science
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    • v.16 no.3
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    • pp.195-198
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    • 2008
  • Karyotypic analysis and FISH (fluorescence in situ hybridization) with 45S and 5S rRNA genes were carried out in Persicaria tinctoria H Gross. The somatic metaphase chromosomes were ranged from 2.25 ${\mu}m$ to 1.50 ${\mu}m$ in length. Chromosome number was 2n = 4x = 40 with the basic number of x = 10. The chromosome complement of the species consisted of 16 pairs of metacentrics (chromososomes 1,2,3,4,6,7,8,9, 10, 11, 12, 13, 15, 18, 19 and 20) and 4 pairs of submetacentrics (chromosome 5, 14, 16 and 17). The karyotype formula was K(2n) = 4x = 32 m + 8 sm. In FISH analysis, three pairs of 45S rRNA gene loci on the terminal region of submetacentrics (chromosomes 5, 16 and 17) and two pairs of 5S rRNA gene loci on the centromeric region of metacentrics (chromosomes 9 and 11) were detected, respectively.

Karyotype Analysis of Five Species of Genus Pulsatilla (할미꽃속 식물 5종의 핵형 분석)

  • Lee, Woo-Kyu;Choi, Hae-Woon;Bang, Jae-Wook
    • Korean Journal of Medicinal Crop Science
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    • v.12 no.6
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    • pp.490-493
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    • 2004
  • Karyotypes were established in five Pulsatilla species from Korea : P. cernua, P. davurica, P. koreana, P. chinensis and P. tongkangensis. The somatic chromosome numbers of five species were all 2n=2x=16 with the basic number of x=8. The chromosome complement of P. cernua consisted of 5 pairs of metacentric, 1 pair of submetacentric and 2 pairs of subtelocentric. P. davurica, P. koreana and P. chinensis consisted of 5 pairs of metacentric and 3 pairs of subtelocentric. P. tongkangensis consisted of 5 pairs of metacentric, 2 pairs of submetacentric, and 1 pair of subtelocentric. Karyotype formulas of P. davurica, P. koreana, and P. chinensis were the same as K (2n) = 2x = 16 = 10m + 6st, while those of P. cernua was K (2n) = 2x = 16 = 10m + 2sm + 4st and P. tongkangensis was K (2n) = 2x = 16 = 10m + 4sm + 2st, respectively.

Cytogenetic and Physiological Studies in Natural Populations of Torreya nucifera (비자나무 천연생집단(天然生集團)의 세포유전학적(細胞遺傳學的) 및 생리학적(生理學的) 연구(硏究))

  • Kim, Yeung Du;Kwon, Yeong Cheol
    • Journal of Korean Society of Forest Science
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    • v.78 no.1
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    • pp.42-54
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    • 1989
  • These studies were carried out to examine the anatomical, karyological characteristics, the variation of isozymes in needle and the frost damage of tissue for the purpose of investigating the relationships among the three natural Torreya nucifera populations of Cheju-do, Namhae, and Mt. Naejang in Korea. The results obtained can be summed up as follows : 1. The numbers of endodermal cells and inner layer cells of mucilage canal of needle conductive tissue were different in each population. The number of those of Cheju-do population was the largest. 2. In somatic chromosome, Namhae population showed one more secondary constriction. The values of $b^{arm}/a^{arm}$ were the same in all the three populations, but Cheju-do population particularly showed a different minimum value. And the karyotype formulas of each population showed difference. 3. During the meiosis, each population showed no significant difference in the irregular phenomena of chromosome. 4. In isoperoxidase and esterase variations of needle, each population showed its particular number and variation of bands. Cheju-do population showed the largest number and greatest variation of bands. 5. Under the same freeging conditions, the frost damage of Mt. Naejang population was comparatively slight, and Cheju-do population suffered from a greater frost damage than the others.

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Karyotype Analysis of Five Iris Species Native to Korea (한국 자생 붓꽃과 식물 5종의 핵형분석)

  • Park, Young-Wook;Kim, Dong-Ming;Hwang, Yoon-Jung;Lim, Ki-Byung;Kim, Hyun-Hee
    • Journal of Plant Biotechnology
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    • v.33 no.1
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    • pp.39-43
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    • 2006
  • A detailed karyotype analysis was carried out in five Korean native Iridaceae species; Iris pseudoacorus, I. pallasii. var. chinensis, I. tectrum, I. dichotoma and Belamcanda chinensis. Chromosome compositions of the five species showed that they have different karyotypic formulae; I. pseudoacorus 2n=34=10m+16smn+8st including two pairs of satellite chromosomes, I. pallasii var. chinensis 2n=40=26m+12sm+2st including two pairs of satellite chromosomes, I. tectrum 2n=30=14m+16sm including five pairs of satellite chromosomes, I. dichotoma 2n=32=22m+10sm including two pairs of satellite chromosomes, and B. chinensis 2n=32=20m+10sm+2st including one pair of satellite chromosomes. These results will supplement the previous cytogenetic reports in Iridaceae species and enhance our understanding on the genetic structure, which will be useful in clarifying the unique characteristics of each species for practical breeding programs for horticultural and pharmaceutical purposes.