Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Karyotypic Analysis and Physical Mapping of rRNA Gene Loci in Persicaria tinctoria

쪽의 핵형분석과 rRNA 유전자의 염색체상 위치

  • Choi, Hae-Woon (School of Biosci. & Biotech., Chungnam Natl. Univ.) ;
  • Lee, Sang-Hoon (School of Biosci. & Biotech., Chungnam Natl. Univ.) ;
  • Kim, Soo-Young (Korea Research Institute of Bioscience and Biotechnolgy (KRIBB)) ;
  • Bang, Jae-Wook (School of Biosci. & Biotech., Chungnam Natl. Univ.)
  • 최혜운 (충남대학교 생명과학부 생물학과) ;
  • 이상훈 (충남대학교 생명과학부 생물학과) ;
  • 김수영 (한국생명공학연구원) ;
  • 방재욱 (충남대학교 생명과학부 생물학과)
  • Published : 2008.06.30
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Abstract

Karyotypic analysis and FISH (fluorescence in situ hybridization) with 45S and 5S rRNA genes were carried out in Persicaria tinctoria H Gross. The somatic metaphase chromosomes were ranged from 2.25 ${\mu}m$ to 1.50 ${\mu}m$ in length. Chromosome number was 2n = 4x = 40 with the basic number of x = 10. The chromosome complement of the species consisted of 16 pairs of metacentrics (chromososomes 1,2,3,4,6,7,8,9, 10, 11, 12, 13, 15, 18, 19 and 20) and 4 pairs of submetacentrics (chromosome 5, 14, 16 and 17). The karyotype formula was K(2n) = 4x = 32 m + 8 sm. In FISH analysis, three pairs of 45S rRNA gene loci on the terminal region of submetacentrics (chromosomes 5, 16 and 17) and two pairs of 5S rRNA gene loci on the centromeric region of metacentrics (chromosomes 9 and 11) were detected, respectively.

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