• Asian-Australasian Journal of Animal Sciences
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• v.27 no.5
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• pp.648-657
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• 2014

Silage making has become a significant method of forage conservation worldwide. To determine how tomato pomace (TP) may be used effectively as animal feed, it was ensilaged for 90 days and microbiology counts, fermentation characteristics and chemical composition of tomato pomace silage (TPS) were evaluated at the 30th, 60th, and 90th days, respectively. In addition, 103 lactic acid bacteria were isolated from TPS. Based on the phenotypic and chemotaxonomic characteristics, 16S rDNA sequence and carbohydrate fermentation tests, the isolates were identified as 17 species namely: Lactobacillus coryniformis subsp. torquens (0.97%), Lactobacillus pontis (0.97%), Lactobacillus hilgardii (0.97%), Lactobacillus pantheris (0.97%), Lactobacillus amylovorus (1.9%), Lactobacillus panis (1.9%), Lactobacillus vaginalis (1.9%), Lactobacillus rapi (1.9%), Lactobacillus buchneri (2.9%), Lactobacillus parafarraginis (2.9%), Lactobacillus helveticus (3.9%), Lactobacillus camelliae (3.9%), Lactobacillus fermentum (5.8%), Lactobacillus manihotivorans (6.8%), Lactobacillus plantarum (10.7%), Lactobacillus harbinensis (16.5%) and Lactobacillus paracasei subsp. paracasei (35.0%). This study has shown that TP can be well preserved for 90 days by ensilaging and that TPS is not only rich in essential nutrients, but that physiological and biochemical properties of the isolates could provide a platform for future design of lactic acid bacteria (LAB) inoculants aimed at improving the fermentation quality of silage.

• Food Science of Animal Resources
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• v.37 no.5
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• pp.752-763
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• 2017

Marine fish skin peptides (FSP) have been widely studied due to their antioxidant and antimicrobial properties. We aimed to use a natural antioxidant, FSP, to replacing synthetic preservatives in a pork patty model, which is safer for human body. Moreover, nano-liposome technology can be applied for masking the fishy smell and improving the stability of this peptide. Therefore, in this study, the effects of FSP and FSP-loaded liposomes (FSPL) on pork patty were evaluated through the tests of thiobarbituric acid reactive substances (TBARS), color, cooking loss, texture, volatile basic nitrogen (VBN), and the pH value, during 14 d of refrigerated ($4^{\circ}C$) storage. The results showed that all FSP-treated patties had lower TBARS values than control patties, which indicated an inhibitory effect of FSP on lipid oxidation. This effect in the patties depended on the FSP concentration. However, FSPL-treated patties showed significantly higher and undesirable TBARS values compared to the control, and this effect depended on the FSPL concentration. None of the physicochemical results showed remarkable changes except the pH and VBN values. Therefore, this study provides evidence that FSP has great potential to inhibit the lipid oxidation of pork patties and is capable of maintaining the quality and extending the shelf life. However, it is necessary to study the application of FSP treatments greater than 3% to improve the antioxidant effect on pork patties and search for other coating materials and technology to reduce the drawbacks of FSP.

• BMB Reports
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• v.38 no.2
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• pp.151-160
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• 2005

By a cDNA array representing 2308 signal transduction related genes, we studied the expression profiles of HeLa cells stably transfected by Hepatitis C virus nonstructural protein 4B (HCV-NS4B). The alterations of the expression of four genes were confirmed by real-time quantitative RT-PCR; and the aldo-keto reductase family 1, member C1 (AKR1C1) enzyme activity was detected in HCV-NS4B transiently transfected HeLa cells and Huh-7, a human hepatoma cell line. Of the 2,308 genes we examined, 34 were up-regulated and 56 were down-regulated. These 90 genes involved oncogenes, tumor suppressors, cell receptors, complements, adhesions, transcription and translation, cytoskeletion and cellular stress. The expression profiling suggested that multiple regulatory pathways were affected by HCV-NS4B directly or indirectly. And since these genes are related to carcinogenesis, host defense system and cell homeostatic mechanism, we can conclude that HCV-NS4B could play some important roles in the pathogenesis mechanism of HCV.

• The Journal of Korean Medicine
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• v.20 no.2
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• pp.12-26
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• 1999

Jing-Yue Jang(張景岳, 1563{\sim}1640$), a medical scalar in the late Ming(明) Dynasty wrote many books such as Leijing(類經), Zhilyilu(質疑錄) and Jingyuequanshu(景岳全書). He was also famouse for the concept of 'Warming and Recuperating(溫補)' and 'Syndrome Differantation Treatment(辨證論治)', Jingyuequanshu was written between 1636 and 1640, and first published in 1700, It can be said that the book was imported from Ming Dynasty of China to Chosun(朝鮮) Dynasty, in what is now South Korea. between 1713 and 1724 In eighteen century. the book was quoted in Euimunbogam(醫門寶鑑), Jejungshinpyun(濟衆新篇) and Magwaheotong(麻科會通). In nineteen century. the book was quoted in Euijongsonik(醫宗損益), Bangyakhappyun(方藥合編), Dongeuisusebowon (東醫壽世保元) and Euigamjungma(醫鑑重磨). The number of quoted prescription and thesis during in nineteen century. is much more than that of eighteen century, Specifically, there are thirty prescriptions of Xinfangbazhen(新方八陣) in Bangyakhappyun, and the concept of 'Nourishing Yang(扶陽)' in Euigamjungma is also based upon Jing-Yue's thesis, This fact shows that we cannot consider the medicine of the late Chosun Dynasty as an abridgement of Dongeuibogam(東醫寶鑑). It is also said that the study focusing on medical books imported from China to Korea in this period is related to understanding the medical progress in the late Chosun Dynasty and the medical intercommunication between Korea and China.

• Journal for History of Mathematics
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• v.23 no.3
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• pp.1-20
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• 2010

Hong Jung Ha(洪正夏, 1684~?) is the greatest mathematician in Chosun dynasty and wrote a mathematics book Gu Il Jib(九一集) which excels in the area of theory of equations including Gou Gu Shu. The purpose of this paper is to find his influence on the history of Chosun mathematics. He belongs to ChungIn(中人) class and works only in HoJo(戶曹) and hence his contact to other mathematicians is limited. Investigating his colleagues and kinship relations including the affinity and consanguinity, we conclude that he gave a great influence to those people and find that three great ChungIn mathematicans Gyung Sun Jing(慶善徵, 1684~?), Hong Jung Ha and Lee Sang Hyuk(李尙爀, 1810~?) are all related through marriage.

• Journal of the Korean Society of Clothing and Textiles
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• v.34 no.8
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• pp.1253-1264
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• 2010

This research is an in-depth study on the differences of cosmetics advertising and brand representation between Korean companies and Chinese companies. In addition, it studies the preferences of cosmetics consumption in Korea and China. To study these topics, two major methods are applied to magazine advertising analysis and consumer research. Analysis objects are the magazine advertisements of the Korean brands Mamond and Laneige, which entered the China market more than 5 years ago; the 64 advertisements are evenly split between Koreans and Chinese. The objects of the survey are 470 females between the ages 20 and 30 (237 from Korea and 233 from China). The results were as follows. First, Chinese advertisements use intense appeal in which the types of advertisement appeal are highly preferred. Second, ordinary models are highly preferred. Second, (on the nationality of the models) Chinese and Korean models are preferred in comparison to western models. Third, (as shown in the survey) Koreans and Chinese preferred magazine advertisements with headlines and copies. Four, blue colors are commonly used in the advertisements; however, the survey shows that the Chinese consumers prefer gray colors. Furthermore, from this study, there is a significant dynamic between the brand image and consumer satisfaction as well as the re-purchase intention.

• Journal of Korean Society for Quality Management
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• v.47 no.4
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• pp.875-894
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• 2019

Purpose: This research is the impact of service quality of Economy Hotels on customer satisfaction and Re-visit Intention in China. It reviews the empirical theories about Economy Hotels at home and abroad, based on SERVPERF, combines with the actual situation of China's Economy Hotels, and then constructs based on the research results at home and abroad. In the subfactor of service quality, "recovery" is added, and the relationship model between the service quality of Economy Hotel, customer satisfaction and Re-visit Intention is designed, in which the mediated effect of "customer satisfaction" between the service quality's subfactor and Re-visit Intentio is explored. Methods: 334 questionnaires were distributed to the check-in guests of three Home Inns in China's first-tier cities (ShangHai,BeiJing,ShenZhen), of which 300 were valid for data analysis. Result: The data analysis results show that the Economy Hotels' subfactor of service quality have a positive impact on customer satisfaction and Re-visit Intention, and customer satisfaction also plays an mediated effect. (partial mediation: tangible, reliability, recovery; full mediation: responsiveness, assurance, empathy) Conclusion: The research puts forward the enlightenment points for the development of Economic Hotels through the differences in the development emphasis and service quality between Economic hotels and other types of hotels, so as to improve the satisfaction and market competitiveness of China's Economic hotels through the research results.

• Journal of Microbiology and Biotechnology
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• v.24 no.9
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• pp.1162-1169
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• 2014

Glyphosate is the active component of the top-selling herbicide, the phytotoxicity of which is due to its inhibition of the shikimic acid pathway. 5-Enolpyruvylshikimate-3-phosphate synthase (EPSPS) is a key enzyme in the shikimic acid pathway. Glyphosate tolerance in plants can be achieved by the expression of a glyphosate-insensitive aroA gene (EPSPS). In this study, we used a PCR-based two-step DNA synthesis method to synthesize a new aroA gene ($aroA_{R.\;leguminosarum}$) from Rhizobium leguminosarum. In vitro glyphosate sensitivity assays showed that $aroA_{R.\;leguminosarum}$ is glyphosate tolerant. The new gene was then expressed in E. coli and key kinetic values of the purified enzyme were determined. Furthermore, we transformed the aroA gene into Arabidopsis thaliana by the floral dip method. Transgenic Arabidopsis with the $aroA_{R.\;leguminosarum}$ gene was obtained to prove its potential use in developing glyphosate-resistant crops.

• Journal of Microbiology and Biotechnology
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• v.27 no.3
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• pp.471-479
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• 2017

White spot syndrome virus (WSSV) is a major threat to the shrimp farming industry and so far there is no effective therapy for it, and thus early diagnostic of WSSV is of great importance. However, at the early stage of infection, the extremely low-abundance of WSSV DNA challenges the detection sensitivity and accuracy of PCR. To effectively detect low-abundance WSSV, here we developed a pre-amplification PCR (pre-amp PCR) method to amplify trace amounts of WSSV DNA from massive background genomic DNA. Combining with normal specific PCR, 10 copies of target WSSV genes were detected from ${\sim}10^{10}$ magnitude of backgrounds. In particular, multiple target genes were able to be balanced amplified with similar efficiency due to the usage of the universal primer. The efficiency of the pre-amp PCR was validated by nested-PCR and quantitative PCR, and pre-amp PCR showed higher efficiency than nested-PCR when multiple targets were detected. The developed method is particularly suitable for the super early diagnosis of WSSV, and has potential to be applied in other low-abundance sample detection cases.

• Journal of Korea Multimedia Society
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• v.9 no.12
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• pp.1529-1541
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• 2006

Moving objects have been widely employed in traffic and logistic applications. Spatio-temporal aggregations mainly describe the moving object's behavior in the spatial data warehouse. The previous works usually express the object moving in some certain region, but ignore the object often moving along as the trajectory. Other researches focus on aggregation and comparison of trajectories. They divide the spatial region into units which records how many times the trajectories passed in the unit time. It not only makes the storage space quite ineffective, but also can not maintain spatial data property. In this paper, a spatio-temporal aggregation index structure for moving object trajectory in constrained network is proposed. An extended B-tree node contains the information of timestamp and the aggregation values of trajectories with two directions. The network is divided into segments and then the spatial index structure is constructed. There are the leaf node and the non leaf node. The leaf node contains the aggregation values of moving object's trajectory and the pointer to the extended B-tree. And the non leaf node contains the MBR(Minimum Bounding Rectangle), MSAV(Max Segment Aggregation Value) and its segment ID. The proposed technique overcomes previous problems efficiently and makes it practicable finding moving object trajectory in the time interval. It improves the shortcoming of R-tree, and makes some improvement to the spatio-temporal data in query processing and storage.