Functional Characterization of aroA from Rhizobium leguminosarum with Significant Glyphosate Tolerance in Transgenic Arabidopsis |
Han, Jing
(Department of Shanghai Key Laboratory of Agricultural Genetics and Breeding, Biotechnology Research Institute of Shanghai Academy of Agricultural Sciences)
Tian, Yong-Sheng (Department of Shanghai Key Laboratory of Agricultural Genetics and Breeding, Biotechnology Research Institute of Shanghai Academy of Agricultural Sciences) Xu, Jing (Department of Shanghai Key Laboratory of Agricultural Genetics and Breeding, Biotechnology Research Institute of Shanghai Academy of Agricultural Sciences) Wang, Li-Juan (Department of Shanghai Key Laboratory of Agricultural Genetics and Breeding, Biotechnology Research Institute of Shanghai Academy of Agricultural Sciences) Wang, Bo (Department of Shanghai Key Laboratory of Agricultural Genetics and Breeding, Biotechnology Research Institute of Shanghai Academy of Agricultural Sciences) Peng, Ri-He (Department of Shanghai Key Laboratory of Agricultural Genetics and Breeding, Biotechnology Research Institute of Shanghai Academy of Agricultural Sciences) Yao, Quan-Hong (Department of Shanghai Key Laboratory of Agricultural Genetics and Breeding, Biotechnology Research Institute of Shanghai Academy of Agricultural Sciences) |
1 | Jin D, Lu W, Ping S, Zhang W, Chen J, Dun B, et al. 2007. Identification of a new gene encoding EPSPS with high glyphosate resistance from the metagenomic library. Curr. Microbiol. 55: 350-355. DOI ScienceOn |
2 | Tian YS, Xiong AS, Xu J, Zhao W, Gao F, Fu XY, et al. 2010. Isolation from Ochrobactrum anthropi of a novel class II 5- enolpyruvylshikimate-3-phosphate synthase with high tolerance to glyphosate. Appl. Environ. Microbiol. 76: 6001-6005. DOI ScienceOn |
3 | Tian YS, Xu J, Peng RH, Xiong AS, Xu H, Zhao W, et al. 2013. Mutation by DNA shuffling of 5-enolpyruvylshikimate-3-phosphate synthase from Malus domestica for improved glyphosate resistance. Plant Biotechnol. J. 11: 829-838. DOI ScienceOn |
4 | Vaithanomsat P, Brown KA. 2007. Isolation and mutation of recombinant EPSP synthase from pathogenic bacteria Pseudomonas aeruginosa. Process Biochem. 42: 592-598. DOI ScienceOn |
5 | Wang HY, Li YF, Xie LX, Xu P. 2003. Expression of a bacterial aroA mutant, aroA-M1, encoding 5-enolpyruvylshikimate-3-phosphate synthase for the production of glyphosateresistant tobacco plants. J. Plant Res. 116: 455-460. DOI |
6 | Xu J, Tian YS, Peng RH, Xiong AS, Zhu B, Jin XF, et al. 2010. AtCPK6, a functionally redundant and positive regulator involved in salt/drought stress tolerance in Arabidopsis. Planta 231: 1251-1260. DOI |
7 | Wang W, Xia H, Yang X, Xu T, Si HJ, Cai XX, et al. 2013. A novel 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase transgene for glyphosate resistance stimulates growth and fecundity in weedy rice (Oryza sativa) without herbicide. New Phytol. 202: 679-688. |
8 | Xiong AS, Peng RH, Liu JG, Zhuang J, Qiao YS, Xu F, et al. 2007. High efficiency and throughput system in directed evolution in vitro of reporter gene. Appl. Microbiol. Biotechnol. 74: 160-168. DOI ScienceOn |
9 | Laguerre G, Mavingui P, Allard MR, Charnay MP, Louvrier P, Mazurier SI, et al. 1996. Typing of rhizobia by PCR DNA fingerprinting and PCR-restriction fragment length polymorphism analysis of chromosomal and symbiotic gene regions: application to Rhizobium leguminosarum and its different biovars. Appl. Environ. Microbiol. 62: 2029-2036. |
10 | Lanzetta PA, Alvarez LJ, Reinach PS, Candia OA. 1979. An improved assay for nanomole amounts of inorganic phosphate. Anal. Biochem. 100: 95-97. DOI ScienceOn |
11 | Masson Boivin C, Giraud E, Perret X, Batut J. 2009. Establishing nitrogen-fixing symbiosis with legumes: how many rhizobium recipes? Trends Microbiol. 17: 458-466. DOI ScienceOn |
12 | Liu CM, McLean PA, Sookdeo CC, Cannon FC. 1991. Degradation of the herbicide glyphosate by members of the family Rhizobiaceae. Appl. Environ. Microbiol. 57: 1799-1804. |
13 | Long SR. 1989. Rhizobium-legume nodulation: life together in the underground. Cell 56: 203-214. DOI ScienceOn |
14 | Cao G, Liu Y, Zhang S, Yang X, Chen R, Zhang Y, et al. 2012. A novel 5-enolpyruvylshikimate-3-phosphate synthase shows high glyphosate tolerance in Escherichia coli and tobacco plants. PLoS One 7: 1-11. |
15 | Xiong AS, Yao QH, Peng RH, Duan H, Li X, Fan HQ, et al. 2006. PCR-based accurate synthesis of long DNA sequences. Nat. Protoc. 1: 791-797. DOI ScienceOn |
16 | Shi XX, Shi SL, Yang J, Wang ZF. 2006. Research advancement in taxonomy of Rhizobium leguminosarum [J]. Grassland Turf. 1: 002. |
17 | Berti PJ, Chindemi P. 2009. Catalytic residues and an electrostatic sandwich that promote enolpyruvyl shikimate 3-phosphate synthase (AroA) catalysis. Biochemistry 48: 3699-3707. DOI ScienceOn |
18 | Chen L, Pradhan S, Evans Jr TC. 2001. Herbicide resistance from a divided EPSPS protein: the split Synechocystis DnaE intein as an in vivo affinity domain. Gene 263: 39-48. DOI ScienceOn |
19 | Comai L, Facciotti D, Hiatt W, Thompson G, Rose R, Stalker D. 1985. Expression in plants of a mutant aroA gene from Salmonella typhimurium confers tolerance to glyphosate. Nature 317: 741-744. DOI ScienceOn |
20 | Della Cioppa G, Bauer SC, Klein BK, Shah DM, Fraley RT, Kishore GM. 1986. Translocation of the precursor of 5- enolpyruvylshikimate-3-phosphate synthase into chloroplasts of higher plants in vitro. Proc. Natl. Acad. Sci. USA 83: 6873-6877. DOI ScienceOn |
21 | Sun YC, Chen YC, Tian ZX, Li FM, Wang XY, Zhang J, et al. 2005. Novel AroA with high tolerance to glyphosate, encoded by a gene of Pseudomonas putida 4G-1 isolated from an extremely polluted environment in China. Appl. Environ. Microbiol. 71: 4771-4776. DOI ScienceOn |
22 | Murashige T, Skoog F. 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant 15: 473-497. DOI |
23 | Funke T, Yang Y, Han H, Healy-Fried M, Olesen S, Becker A, Schonbrunn E. 2009. Structural basis of glyphosate resistance resulting from the double mutation Thr97Ile and Pro101Ser in 5-enolpyruvylshikimate-3-phosphate synthase from Escherichia coli. J. Biol. Chem. 284: 9854-9860. DOI ScienceOn |
24 | He M, Nie YF, Xu P. 2003. A T42M substitution in bacterial 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) generates enzymes with increased resistance to glyphosate. Biosci. Biotechnol. Biochem. 67: 1405-1409. DOI ScienceOn |
25 | Maj D, Wielbo J, Marek-Kozaczuk M, Skorupska A. 2010. Response to flavonoids as a factor influencing competitiveness and symbiotic activity of Rhizobium leguminosarum. Microbiol. Res. 165: 50-60. DOI ScienceOn |
26 | Murray EE, Lotzer J, Eberle M. 1989. Codon usage in plant genes. Nucleic Acids Res. 17: 477-498. DOI |
27 | Padgette S, Huynh Q, Aykent S, Sammons R, Sikorski J, Kishore G. 1988. Identification of the reactive cysteines of Escherichia coli 5-enolpyruvylshikimate-3-phosphate synthase and their nonessentiality for enzymatic catalysis. J. Biol. Chem. 263: 1798-1802. |
28 | Park H, Hilsenbeck JL, Kim HJ, Shuttleworth WA, Park YH, Evans JN, Kang C. 2004. Structural studies of Streptococcus pneumoniae EPSP synthase in unliganded state, tetrahedral intermediate bound state and S3PGLP-bound state. Mol. Microbiol. 51: 963-971. DOI ScienceOn |
29 | Peng RH, Tian YS, Xiong AS, Zhao W, Fu XY, Han HJ, et al. 2012. A novel 5-enolpyruvylshikimate-3-phosphate synthase from Rahnella aquatilis with significantly reduced glyphosate sensitivity. PLoS One 7: e39579. DOI |
30 | Young JPW, Crossman LC, Johnston AW, Thomson NR, Ghazoui ZF, Hull KH, et al. 2006. The genome of Rhizobium leguminosarum has recognizable core and accessory components. Genome Biol. 7: R34. DOI |
31 | Zhang X, Henriques R, Lin SS, Niu QW, Chua NH. 2006. Agrobacterium-mediated transformation of Arabidopsis thaliana using the floral dip method. Nat. Protoc. 1: 641-646. DOI ScienceOn |
32 | Schonbrunn E, Eschenburg S, Shuttleworth WA, Schloss JV, Amrhein N, Evans JN, Kabsch W. 2001. Interaction of the herbicide glyphosate with its target enzyme 5- enolpyruvylshikimate 3-phosphate synthase in atomic detail. Proc. Natl. Acad. Sci. USA 98: 1376-1380. DOI ScienceOn |
33 | Peng RH, Xiong AS, Yao QH. 2006. A direct and efficient PAGE-mediated overlap extension PCR method for gene multiple-site mutagenesis. Appl. Microbiol. Biotechnol. 73: 234-240. DOI ScienceOn |
34 | Bentley R, Haslam E. 1990. The shikimate pathway - a metabolic tree with many branches. Crit. Rev. Biochem. Mol. Biol. 25: 307-384. DOI ScienceOn |
35 | Priestman MA, Healy ML, Funke T, Becker A, Schönbrunn E. 2005. Molecular basis for the glyphosate-insensitivity of the reaction of 5-enolpyruvylshikimate 3-phosphate synthase with shikimate. FEBS Lett. 579: 5773-5780. DOI ScienceOn |