• 한국수정란이식학회지
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• 제33권3호
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• pp.129-137
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• 2018

Acute vascular rejection has been known as a main barrier occurring in a xenograted tissue of alpha 1,3-galactosyltransferase knock-out (GalT KO) pig into a non-human primate (NHP). Adenosine which is a final metabolite following sequential hydrolysis of nucleotide by ecto-nucleotidases such as CD39 and CD73, act as a regulator of coagulation, and inflammation. Thus xenotransplantation of CD39 and CD73 expressing pig under the GalT KO background could lead to enhanced survival of recipient NHP. We constructed a human CD39 and CD73 expression cassette designed for endothelial cell-specific expression using porcine Icam2 promoter (pIcam2-hCD39/hCD73). We performed isolation of endothelial cells (pAEC) from aorta of 4 week-old GalT KO and membrane cofactor protein expressing pig ($GalT^{-MCP/-MCP}$). We were able to verify that isolated cells were endothelial-like cells using immunofluorescence staining analysis with von Willebrand factor antibody, which is well known as an endothelial maker, and tubal formation assay. To find optimal condition for efficient transfection into pAEC, we performed transfection with GFP expression vector using four programs of nucleofection, M-003, U-023, W-023 and Y-022. We were able find that the program W-023 was optimal for pAEC with regard to viability and transfection efficiency by flow cytometry and fluorescent microscopy analyses. Finally, we were able to obtain $GalT^{-MCP/-MCP}/CD39/CD73$ pAEC expressing CD39 and CD73 at levels of 33.3% and 26.8%, respectively. We suggested that pACE isolated from $GalT^{-MCP/-MCP}$ pig might be provided as a basic resource to understand biochemical and molecular mechanisms of the rejections and as an alternative donor cells to generate $GalT^{-MCP/-MCP}/CD39/CD73$ pig expressing CD39 and CD73 at endothelial cells.

• 한국항공우주학회지
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• 제48권2호
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• pp.127-134
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• 2020

고초음속 항공기는 초음속 비행 중 공력 가열에 의하여 높은 온도 환경에 노출되기 때문에 동체 및 날개 구조물은 더블 패널 형태의 열 차폐 구조로 설계하여 기체 내부로 높은 온도의 열이 전달되는 것을 막는다. 얇은 두께의 더블 패널 외피는 초음속 항공기의 고출력 엔진 소음과 제트 유동에 의한 음향 하중에 노출되어 음향 피로 손상이 발생할 수 있다. 따라서 열음향 복합 하중을 받는 초음속 항공기 외피 구조의 거동 확인과 피로수명 예측이 필요하다. 본 논문에서는 열음향 복합 하중을 모사할 수 있는 열음향 시험 장치를 설계/제작하여 열음향 하중이 적용되는 티타늄 시편의 열음향 시험을 수행하였다. 열음향 복합 하중에 의한 구조물의 동적 거동을 확인하였으며, 시편 단위 열음향 시험 결과와 유한요소해석 결과를 비교하여 해석 모델을 검증하였다.

• 한국수정란이식학회지
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• 제28권3호
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• pp.193-198
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• 2013

This study was designed to evaluate the effect of L-cysteine on sperm characteristics and oocyte cleavage in vitro in Korean native cattle. For this study, the freezing of diluted semen were added with Triladyl containing 20% egg-yolk and/or 0, 5, 10 and 20 mM L-cysteine before cryopreservation. The viability in frozen-thawed sperm were estimated by SYBR14/PI double stain, acrosome damage with FITC-PNA, mitochondria intact with Rhodamin123 and hydrogen peroxide($H_2O_2$) level with carboxy-DCFDA by flow-cytometry. The developmental capacity was also assessed with cleavage rates in oocytes fertilized in vitro by frozen-thawed sperm. In results, the sperm viability was significantly increased in 10 mM and 20 mM concentrations of L-cysteine than other groups (p<0.05). In addition, acrosome damage was significantly decreased in 10 mM and 20 mM concentrations of L-cysteine than other groups (p<0.05). The mitochondria intact was also significantly increased in 10 mM and 20 mM concentrations of L-cysteine than other groups (p<0.05). On the other hand, the cleavage rates were significantly increased in 0 mM, 5 mM and 10 mM groups than 20 mM concentration of L-cysteine (p<0.05). The oocyte degeneration of oocytes were significantly decreased in 0 mM, 5 mM and 10 mM groups than in 20 mM L-cysteine group (P<0.05). However, there are no significantly differences among the L-cysteine treatment groups. We suggest that concentration of 10 mM L-cysteine have beneficial impact for sperm cryopreserved in Korean native cattle. This result also could be recommended for artificial insemination program if supported by an improvement in the fertility results and required further study.

• 한국터널지하공간학회 논문집
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• 제17권2호
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• pp.141-151
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• 2015

터널굴착의 효율성 증진 및 굴착시 발생하는 진동 저감을 위해 연마재 투입형 워터젯 시스템을 이용한 새로운 형태의 암반 굴착 방식이 개발되어 적용 중에 있다. 연마재 투입형 워터젯 시스템을 이용한 암반 굴착 방식에 있어서, 적절한 양의 연마재를 투입하는 것은 절삭 성능뿐만 아니라 전체 공정의 경제성 향상을 위해서도 매우 중요하다. 본 연구에서는, 다양한 공정 변수들 중 터널 굴착용 워터젯 시스템에서 특히 중요한 공정 변수들인 기하학적 변수(연마재 탱크(또는 호퍼) 높이, 연마재 투입관의 구배, 연마재 투입관의 길이, 연마재 투입관의 굴곡도), 연마재 변수(연마재 입자 크기), 젯 에너지 변수(수압, 유량)이 연마재 투입량 및 연마재가 믹싱 챔버 내로 흡입될 때 발생하는 흡입 압력에 미치는 영향을 규명하기 위하여 다양한 조건에서 실험을 수행하고 그 결과를 분석하였다. 또한, 실험적 연구를 통하여 연마재 투입량이 절삭 성능에 미치는 영향을 논의하였다. 본 연구 결과를 바탕으로 주요 공정 변수들을 조절하여 적절한 연마재 투입량을 유지하면 터널 굴착용 워터젯의 절삭 성능을 극대화할 수 있을 것으로 기대된다.

• 천문학회지
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• 제36권3호
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• pp.81-87
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• 2003

It is suggested that a flying-by star in a hot accretion disk may cool the hot accretion disk by the Comptonization of the stellar emission. Such a stellar cooling can be observed in the radio frequency regime since synchrotron luminosity depends strongly on the electron temperature of the accretion flow. If a bright star orbiting around the supermassive black hole cools the hot disk, one should expect a quasi-periodic modulation in radio, or even possible an anti-correlation of luminosities in radio and X-rays. Recently, the unprecedentedly accurate infrared imaging of the Sagittarius A$\ast$ for about ten years enables us to resolve stars around it and thus determine orbital parameters of the currently closest star S2. We explore the possibility of using such kind of observation to distinguish two quite different physical models for the central engine of the Sagittarius A$\ast$, that is, a hot accretion disk model and a jet model. We have attempted to estimate the observables using the observed parameters of the star S2. The relative difference in the electron temperature is a few parts of a thousand at the epoch when the star S2 is near at the pericenter. The relative radio luminosity difference with and without the stellar cooling is also small of order $10^{-4}$, particularly even when the star S2 is near at the pericenter. On the basis of our findings we tentatively conclude that even the currently closest pass of the star S2 is insufficiently close enough to meaningfully constrain the nature of the Sagittarius A$\ast$ and distinguish two competing models. This implies that even though Bower et al. (2002)have found no periodic radio flux variations in their data set from 1981 to 1998, which is naturally expected from the presence of a hot disk, a hot disk model cannot be conclusively ruled out. This is simply because the energy bands they have studied are too high to observe the effect of the star S2 even if it indeed interacts with the hot disk. In other words, even if there is a hot accretion disk the star like S2 has imprints in the frequency range at v $\le$ 100 MHz.

• 한국추진공학회:학술대회논문집
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• 한국추진공학회 2004년도 제22회 춘계학술대회논문집
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• pp.48-54
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• 2004

The effects of injector spacing s and injector diameter d on mixing are numerically investigated in supersonic combustor with perpendicular injection behind a backward-facing step. Simulations are reported for airstream Mach number of 2.4. Parameters are changed on following 4 cases to investigate the effects of injector configuration on mixing efficiency $\eta_m$. In the case of varying d or s, dynamic pressure ratio $Rq(=(pu^2)_j/(pu^2)_a)$ is also varied to keep bulk equivalence ratio $\Phi({\oe})Rq.d^2/s)$ constant. (l) Injector spacing s is varied at constant $\Phi$=0.5, 1, 2 for injector diameter d=6mm. In the case of $\Phi$=1, $\eta_m$ has its maximum value at s=24mm. The reason is that increase of $\eta_m$. , by widening spacing at Rq=constant competes with decrease of $\eta_m$ by increasing Rq at s=constant. When spacing is narrow, the flow field of vicinity of injector becomes two-dimensional because adjacent jets interferes each other. By widening spacing, air is easily entrained by three-dimensional effect. This mechanism also appears in the case of $\Phi$=0.5, 2 for d=6mm, and $\eta_m$. reaches its maximum value at s=24mm for $\Phi$=0.5 and at s=42mm for $\Phi$=2. (2) In the case of injector diameter d varied at $\Phi$=1 for s=30mm, $\eta_m$. has its maximum value at d=3mm. The reason is that decrease of $\eta_m$ by increasing injector diameter competes with increase of $\eta_m$ by decreasing Rq at d=constant.(3) In the case of s varied at $\Phi$=0.5, 1,2 for d=3mm, the injector spacing at which mixing efficiency has its maximum value is s= 18mm for $\Phi$=0.5, s=24mm for $\Phi$=1, s=24mm for $\Phi$=2. Therefore it is found that d=3mm and s=24mm can be optimum configuration over a range of $\Phi$=0.5~2.(4) The effect of h on the optimum spacing is investigated. s is varied for d=6mm at step height h=4, 6, 8mm. The simulation results do not show significant change on the step height.

• 한국수정란이식학회지
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• 제30권3호
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• pp.121-128
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• 2015

The objective of this study was to evaluate the efficiency of sperm cryosurvival in boar sperm separated by Percoll containing antioxidant enzymes. The boar semen was collected into a pre-warmed ($37^{\circ}C$) thermos bottle by gloved-hand method and was separated by 65% Percoll with superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH) before freezing. The frozen sperm was thawed at $38.5^{\circ}C$ for 45 sec in water-bath for sperm characteristic analysis. The sperm were estimated with SYBR14/PI double staining for viability, FITC-PNA/PI double staining for acrosome reaction, Rhodamine123/PI double staining for mitochondrial integrity and were analyzed using flow cytometry. In results, sperm viability, acrosome reaction and mitochondrial integrity were improved in separated sperm groups compared with unseparated sperm by Percoll (UP) group. Especially, viability was significantly higher in sperm separated by Percoll containing 400 IU CAT group compared with other groups (P<0.05). And acrosome reaction was decreased in sperm separated by Percoll with 300 IU SOD, 400 IU CAT and 0.5 mM GSH groups compared with other groups, however, there were no significantly difference mitochondrial integrity among sperm separated by Percoll with antioxidant enzymes. In conclusion, we suggest that use of Percoll containing antioxidant enzymes for sperm separation will be beneficial for sperm cryopreservation in pigs.

• 한국수정란이식학회지
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• 제29권4호
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• pp.385-391
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• 2014

Objective of this study was to investigate the effect of nicotinic acid (NA) on the characteristics in fresh semen of miniature pig. We evaluated viability, acrosome reaction and mitochondrial integrity of sperm on 0, 3, 7 and 10 days during storage period with nicotinic acid. As results, the survival rate of sperm in 15 mM NA (day 3, $87.8{\pm}1.2%$; day 5, $84.0{\pm}2.7%$; day 7, $82.2{\pm}0.9%$) and 30 mM NA (day 3, $87.7{\pm}0.3%$; day 5, $84.4{\pm}2.5%$; day 7, $82.3{\pm}0.7%$) groups were higher than control and 5 mM NA groups in 3, 7 and 10 days of semen storage. The NA-treated sperm on 10 day was used day for observing acrosome integrity. The survival sperm with acrosome reaction was higher in 30 mM NA group (day 3, $2.7{\pm}0.2%$; day 5, $3.3{\pm}0.6%$; day 7, $11.4{\pm}0.3%$) than in the control, significantly (P<0.05). Moreover, the live sperm with mitochondrial integrity was higher in whole treatment groups of NA than control group, significantly (P<0.05). Specially, most mitochondrial integrity on 10 day of semen storage was significantly higher in 30 mM NA group ($90.2{\pm}1.6%$) than other treatment groups (control, $81.8{\pm}3.1%$; 5 mM NA, $83.4{\pm}3.0%$; 15 mM NA, $89.1{\pm}0.7%$, P<0.05). In conclusion, supplement of NA in liquid semen of miniature pig can improve and maintain semen quality, such as viability, acrosome reaction, and mitochondria integrity.

• 한국수정란이식학회지
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• 제32권3호
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• pp.73-79
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• 2017

Transplantation is considered to be a very useful approach to improve human welfare and to prolong life-span. Heterologous organ transplantation using pig organs which are similar to human beings and easy to make mass-production has known as one of the alternatives. To ensure potential usage of the pig organ for transplantation application, it is essentially required to generate transgenic pig modifying immuno-related genes. Previously, we reported production of heterozygous ${\alpha}1,3$-galactosyltransferase (GalT) knock-out and human membrane cofactor protein (MCP) expressing pig ($GalT^{-MCP/+}$), which is enforced for suppression of hyperacute and acute immunological rejection. In this study, we reported generation of homozygous pig ($GalT^{-MCP/-MCP}$) by crossbreeding $GalT^{-MCP/+}$ pigs. Two female founders gave birth to six of $GalT^{-MCP/-MCP}$, and seven $GalT^{-MCP/+}$ pigs. We performed quantitative real-time PCR, western blot, and flow cytometry analyses to confirm GalT and MCP expression. We showed that fibroblasts of the $GalT^{-MCP/-MCP}$ pig do not express GalT and its product Gal antigen, while efficiently express MCP. We also showed no expression of GalT, otherwise expression of MCP at heart, kidney, liver and pancreas of transgenic pig. Taken together, we suggest that the $GalT^{-MCP/-MCP}$ pig is a useful candidate to apply xenotransplantation study.

• 공업화학
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• 제19권3호
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• pp.270-276
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• 2008

$TiO_2$의 가장 큰 특징은 광촉매적 특성을 들 수 있으나 순수한 $TiO_2$는 자외선 영역에서만 활성을 보이는 단점이 있다. 단점을 보완하고자 본 연구에서는 초고온, 고활성을 이용한 열플라즈마 공정으로 질소가 도핑된 $TiO_2$를 합성하여 $TiO_2$의 광촉매적 특성을 높이고자 하였다. 직류 플라즈마 제트를 이용하여 비금속이온인 질소와 반응 가스인 산소를 $TiCl_4$와 함께 플라즈마 반응기 안에서 반응시켜 질소가 도핑된 $TiO_2$ 나노 분말을 합성하였다. 합성 조건으로 질소의 유량을 변화하였다. 합성 변수에 따른 입자의 상조성, 크기를 분석하였고 아세트알데히드와 곰팡이를 광분해하는 실험을 통해 광촉매 활성을 살펴보았다. 한편 $TiO_2$의 분말 상태와 코팅된 상태의 광촉매 특성을 비교하고자 합성한 분말의 스핀 코팅과 PLD (Pulsed Laser Deposition)을 통해 $TiO_2$를 코팅하였다. 아세트알데히드 분해 실험의 결과 질소가 도핑된 $TiO_2$ 분말의 경우가 순수한 $TiO_2$ 분말에 비해 가시영역에서의 광촉매 활성이 두 배 이상 뛰어난 것을 확인하였으며, 곰팡이 분해 실험 결과 역시 질소가 도핑된 $TiO_2$ 분말에 곰팡이가 분해되는 것을 확인하였다. 분말과 필름을 제조하여 메틸렌블루 광분해 실험한 결과 분말의 경우 100% $TiO_2$입자가 메틸렌블루 분해에 이용되며, 반면 스핀 코팅의 경우 바인더의 함량 때문에 20~30%의 $TiO_2$만이 분해에 이용되기 때문에, 분말의 경우 초기 30 mL 메틸렌블루를 한번에 분해할 수 있었다.