Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Development of α1,3-galactosyltransferase Inactivated and Human Membrane Cofactor Protein Expressing Homozygous Transgenic Pigs for Xenotransplantation

이종이식에 활용할 α1,3-galactosyltransferase 비활성화 및 Membrane Cofactor Protein 발현 동형접합 형질전환 돼지 개발

  • Lee, Gunsup (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Park, Sang Hyoun (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Lee, Haesun (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Ji, Soo-Jeong (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Lee, Joo Yung (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Byun, Sung-June (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Hwang, Seongsoo (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Kim, Kyung Woon (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Ock, Sun A (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Oh, Keon Bong (Animal Biotechnology Division, National Institute of Animal Science, RDA)
  • 이건섭 (국립축산과학원 동물바이오공학과) ;
  • 박상현 (국립축산과학원 동물바이오공학과) ;
  • 이해선 (국립축산과학원 동물바이오공학과) ;
  • 지수정 (국립축산과학원 동물바이오공학과) ;
  • 이주영 (국립축산과학원 동물바이오공학과) ;
  • 변승준 (국립축산과학원 동물바이오공학과) ;
  • 황성수 (국립축산과학원 동물바이오공학과) ;
  • 김경운 (국립축산과학원 동물바이오공학과) ;
  • 옥선아 (국립축산과학원 동물바이오공학과) ;
  • 오건봉 (국립축산과학원 동물바이오공학과)
  • Received : 2017.07.31
  • Accepted : 2017.09.08
  • Published : 2017.09.30
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Abstract

Transplantation is considered to be a very useful approach to improve human welfare and to prolong life-span. Heterologous organ transplantation using pig organs which are similar to human beings and easy to make mass-production has known as one of the alternatives. To ensure potential usage of the pig organ for transplantation application, it is essentially required to generate transgenic pig modifying immuno-related genes. Previously, we reported production of heterozygous ${\alpha}1,3$-galactosyltransferase (GalT) knock-out and human membrane cofactor protein (MCP) expressing pig ($GalT^{-MCP/+}$), which is enforced for suppression of hyperacute and acute immunological rejection. In this study, we reported generation of homozygous pig ($GalT^{-MCP/-MCP}$) by crossbreeding $GalT^{-MCP/+}$ pigs. Two female founders gave birth to six of $GalT^{-MCP/-MCP}$, and seven $GalT^{-MCP/+}$ pigs. We performed quantitative real-time PCR, western blot, and flow cytometry analyses to confirm GalT and MCP expression. We showed that fibroblasts of the $GalT^{-MCP/-MCP}$ pig do not express GalT and its product Gal antigen, while efficiently express MCP. We also showed no expression of GalT, otherwise expression of MCP at heart, kidney, liver and pancreas of transgenic pig. Taken together, we suggest that the $GalT^{-MCP/-MCP}$ pig is a useful candidate to apply xenotransplantation study.

Keywords

References

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