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Draft genome sequence of Bacteroides sp. KGMB 02408 isolated from a healthy Korean feces (건강한 한국인 분변으로부터 분리된 Bacteroides sp. KGMB 02408 균주의 유전체 염기서열 초안)

  • Yu, Seung Yeob;Kim, Ji-Sun;Oh, Byeong Seob;Ryu, Seoung Woo;Park, Seung-Hwan;Kang, Se Won;Park, Jam-Eon;Choi, Seung-Hyeon;Han, Kook-Il;Lee, Keun Chul;Eom, Mi Kyung;Suh, Min Kuk;Kim, Han Sol;Lee, Dong Ho;Yoon, Hyuk;Kim, Byung-Yong;Lee, Je Hee;Lee, Jung-Sook;Lee, Ju Huck
    • Korean Journal of Microbiology
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    • v.55 no.3
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    • pp.296-299
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    • 2019
  • The genus of Bacteroides has been isolated from vertebrate animal feces. Bacteroides sp. KGMB 02408 was isolated from fecal samples obtained from a healthy Korean. The wholegenome sequence of Bacteroides sp. KGMB 02408 was analyzed using the PacBio Sequel platform. The genome comprises a 5,771,427 bp chromosome with a G + C content of 39.50%, 5,005 total genes, 18 rRNA genes, and 74 tRNA genes. Furthermore, we found that strain KGMB 02408 had some genes for oxidoreductases and menaquinone biosynthesis in its genome based on the result of genome analysis.

Strategy for Countermeasures the NK's Nuclear Issue Using Offset Strategy and the 4th Industrial Revolution Technologies (상쇄전략과 4차 산업혁명 기술혁신을 적용한 북핵 대응방안)

  • Park, Jae-wan
    • Korea and Global Affairs
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    • v.3 no.1
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    • pp.77-106
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    • 2019
  • The purpose of this paper is to propose measures to respond to NK's nuclear threat by applying the concept of offset strategy in the US and the fourth industrial revolution technology innovation. Through this study, the concept, technology, and organization were examined to find measures to counter the NK's nuclear threat. The concept was to review the US offset strategy and consider the strategy of paralyzing the enemy's center as an operational concept. And the technologies that can support the 4th Industrial revolution and the 3rd US offset strategy. The organization analyzed the implications for the reform of the National Defense Reform 2.0 and the reorganization of the acquisition of the US DoD. Through the US offset strategy review, it is necessary to reconcile the strategic concepts of Korea and the US, and the interoperability of technologies and the cooperation fields of the ROK-US alliance. In addition, it is necessary to strengthen the capability of responding to NK's nuclear threat in connection with the 4th Industrial Revolution and the National Defense Reform 2.0 promotion. It is necessary to develop the concept of operational performance applying the new paradigm for the NK's nuclear issue and to apply advanced science and technology. And that it is necessary to organize effectively in conjunction with the National Defense Reform 2.0.

Draft genome sequence of Olsenella sp. KGMB 04489 isolated from healthy Korean human feces (건강한 한국인 분변으로부터 분리된 Olsenella sp. KGMB 04489 균주의 유전체 염기서열 초안)

  • Han, Kook-Il;Kang, Se Won;Kim, Ji-Sun;Lee, Keun Chul;Eom, Mi Kyung;Suh, Min Kuk;Park, Seung-Hwan;Lee, Ju Huck;Park, Jam-Eon;Oh, Byeong Seob;Yu, Seung Yeob;Choi, Seung-Hyeon;Lee, Dong Ho;Yoon, Hyuk;Kim, Byung-Yong;Yang, Seung-Jo;Lee, Jung-Sook
    • Korean Journal of Microbiology
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    • v.54 no.4
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    • pp.456-459
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    • 2018
  • The genus of Olsenella has been isolated from vertebrate animal mouth, rumen, and feces. Olsenella sp. KGMB 04489 was isolated from fecal samples obtained from a healthy Korean. The whole-genome sequence of Olsenella sp. KGMB 04489 was analyzed using the PacBio Sequel platform. The genome comprises a 2,108,034 bp chromosome with a G + C content of 65.50%, 1,838 total genes, 13 rRNA genes, and 52 tRNA genes. Also, we found that strain KGMB 04489 had some genes for hydrolysis enzymes, and antibiotic biosynthesis and resistance in its genome based on the result of genome analysis.

Effects of mulberry fruit juice powder on inflammation and microRNA-132/143 regulation in 3T3-L1 adipocytes (뽕나무 열매 착즙 분말이 3T3-L1 지방세포의 염증 및 microRNA-132/143 조절에 미치는 영향)

  • Lee, Mak-Soon;Kim, Yangha
    • Journal of Nutrition and Health
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    • v.54 no.5
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    • pp.448-458
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    • 2021
  • Purpose: Mulberry (Morus alba L.) fruit is widely grown in Asia and consumed as fresh fruit, jam, and juices. The fruit has beneficial health effects, including anti-diabetic, anti-tumor, and anti-obesity properties. However, the mechanisms by which mulberry fruit juice powder (MJ) regulates inflammatory microRNAs (miRs) are not yet known. This study investigated the effect of mulberry fruit juice powder on the regulation of inflammation and miR-132/143 during 3T3-L1 adipocyte differentiation. Methods: The 3T3-L1 cells were induced to differentiate for 2 days and then treated with various concentrations of MJ for 7 days. Cytotoxicity was determined by evaluating cell viability using a water-soluble tetrazolium salt-8 assay kit. Intracellular lipid accumulation was evaluated by oil-red O staining. The levels of the expression of genes involved in adipogenesis and inflammation, and miR-132/143 were measured by quantitative real-time polymerase chain reactions. Results: MJ showed no cytotoxic effect on 3T3-L1 adipocytes at concentrations below 100 ng/mL. Intracellular lipid accumulation was reduced by MJ treatment at concentrations of 100 ng/mL. The messenger RNA (mRNA) levels of proliferator-activated receptor-γ, cytosine-cytosine-adenosine-adenosine-thymidine/enhancer-binding protein-α, and adipocyte protein 2, which are involved in adipogenesis, were suppressed by MJ. A reduction was also seen in mRNA levels of genes related to the inflammatory response, such as tumor necrosis factor-α, interleukin-6, monocyte chemoattractant protein-1, and inducible nitric oxide synthase. The expression of the inflammatory miR-132 and miR-143 was also decreased by MJ. Conclusion: These results suggest that MJ may suppress adipogenesis and inflammation through the regulation of miR-132/143 expression in 3T3-L1 adipocytes. Thus, MJ may be useful as a food agent that prevents obesity-associated inflammation.

Design of Over-sampled Channelized DRFM Structure in order to Remove Interference and Prevent Spurious Signal (간섭 제거 및 스퓨리어스 방지를 위한 오버샘플링 된 채널화 DRFM 구조 설계)

  • Kim, Yo-Han;Hong, Sang-Guen;Seo, Seung-Hun;Jo, Jung-Hun
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.26 no.8
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    • pp.1213-1221
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    • 2022
  • In Electronic Warfare, the need to develop a jamming system that protects our location information from enemy radar is constantly increasing. The jamming system normally uses wide-band DRFM(Digital Radio Frequency Memory) that processes the entire bandwidth at once. However, it is difficult to jam if there is a CW(Continuous Wave) interference signal in the band. Recently, instead of wide-band signal processing, a structure using a filter bank that divides the entire band into several sub-bands and processes each sub-band independently has been proposed. Although it is possible to handle interference signal through the filter bank structure, spurious signal occurs when the signal is received at a boundary frequency between sub-bands. Spurious signal makes a output power of jamming signal distributed, resulting in lower JSR(Jamming to Signal Ratio) and less jamming effect. This paper proposes an over-sampled channelized DRFM structure that enables interference response and prevents spurious signal for sub-band boundary frequency input.

Training a semantic segmentation model for cracks in the concrete lining of tunnel (터널 콘크리트 라이닝 균열 분석을 위한 의미론적 분할 모델 학습)

  • Ham, Sangwoo;Bae, Soohyeon;Kim, Hwiyoung;Lee, Impyeong;Lee, Gyu-Phil;Kim, Donggyou
    • Journal of Korean Tunnelling and Underground Space Association
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    • v.23 no.6
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    • pp.549-558
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    • 2021
  • In order to keep infrastructures such as tunnels and underground facilities safe, cracks of concrete lining in tunnel should be detected by regular inspections. Since regular inspections are accomplished through manual efforts using maintenance lift vehicles, it brings about traffic jam, exposes works to dangerous circumstances, and deteriorates consistency of crack inspection data. This study aims to provide methodology to automatically extract cracks from tunnel concrete lining images generated by the existing tunnel image acquisition system. Specifically, we train a deep learning based semantic segmentation model with open dataset, and evaluate its performance with the dataset from the existing tunnel image acquisition system. In particular, we compare the model performance in case of using all of a public dataset, subset of the public dataset which are related to tunnel surfaces, and the tunnel-related subset with negative examples. As a result, the model trained using the tunnel-related subset with negative examples reached the best performance. In the future, we expect that this research can be used for planning efficient model training strategy for crack detection.

Control Method for the Number of Travel Hops for the ACK Packets in Selective Forwarding Detection Scheme (선택적 전달 공격 탐지기법에서의 인증 메시지 전달 홉 수 제어기법)

  • Lee, Sang-Jin;Kim, Jong-Hyun;Cho, Tae-Ho
    • Journal of the Korea Society for Simulation
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    • v.19 no.2
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    • pp.73-80
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    • 2010
  • A wireless sensor network which is deployed in hostile environment can be easily compromised by attackers. The selective forwarding attack can jam the packet or drop a sensitive packet such as the movement of the enemy on data flow path through the compromised node. Xiao, Yu and Gao proposed the checkpoint-based multi-hop acknowledgement scheme(CHEMAS). In CHEMAS, each path node enable to be the checkpoint node according to the pre-defined probability and then can detect the area where the selective forwarding attacks is generated through the checkpoint nodes. In this scheme, the number of hops is very important because this parameter may trade off between energy conservation and detection capacity. In this paper, we used the fuzzy rule system to determine adaptive threshold value which is the number of hops for the ACK packets. In every period, the base station determines threshold value while using fuzzy logic. The energy level, the number of compromised node, and the distance to each node from base station are used to determine threshold value in fuzzy logic.

The Self-Perception and Science Teaching Implementation of Elementary School Teacher Aiming for Student-centered Inquiry Classes -Focusing on RTOP Analysis of the Elementary School 'Temperature and Heat' Unit- (학생 중심 탐구수업을 지향하는 초등교사의 과학수업에 대한 자기인식과 실행 -초등학교 '온도와 열' 단원에 대한 RTOP 분석을 중심으로-)

  • Chaeyeon Shin;Hyojoon Kim
    • Journal of Science Education
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    • v.47 no.1
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    • pp.88-106
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    • 2023
  • This study aims to investigate the disparity between the teacher's perception of student-centered inquiry classes and the actual implementation of such practices. Specifically, we compared an elementary science teacher's self-perception of her science lessons with the observers' evaluation using the Reformed Teaching Observation Protocol (RTOP) of the "Temperature and Heat" unit. Research data were collected through classroom teaching survey, interview, and science lessons video which were analyzed using the RTOP. As a result of the study, the teacher recognized that she was practicing inquiry-oriented/student-centered classes, but the results judged by the RTOP score were found to be transitional/student-affected classes by a slight difference. Teacher H planned and practiced classes based on a high understanding and content knowledge of the curriculum and created a science classroom culture that promotes active interaction among students as well as students and teachers. However, teacher-led aspects were still emphasized in teaching design and implementation, and the project theme and content were inappropriate to improve the quality of students' science inquiry experience. In the end, the slight difference between teacher's perception of inquiry-oriented/student-centered classes and actual implementation is related to how student-centered "lesson design" is and how to plan and implement classes supported by "procedural knowledge" for students' experience in the science inquiry process. These results indicate that the teacher's self-evaluation alone is not enough to determine whether the teacher's intentions and efforts are actually being implemented, and that it is necessary to conduct objective analysis, evaluation, and discuss the results of science classes by the external observers.

Study on Advisory Safety Speed Model Using Real-time Vehicular Data (실시간 차량정보를 이용한 안전권고속도 산정방안에 관한 연구)

  • Jang, JeongAh;Kim, HyunSuk
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.30 no.5D
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    • pp.443-451
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    • 2010
  • This paper proposes the methodology about advisory safety speed based on real-time vehicular data collected from highway. The proposed model is useful information to drivers by appling seamless wireless communication and being collected from ECU(Engine Control Unit) equipment in every vehicle. Furthermore, this model also permits the use of realtime sensing data like as adverse weather and road-surface data. Here, the advisory safety speed is defined "the safety speed for drivers considering the time-dependent traffic condition and road-surface state parameter at uniform section", and the advisory safety speed model is developed by considering the parameters: inter-vehicles safe stopping distance, statistical vehicle speed, and real-time road-surface data. This model is evaluated by using the simulation technique for exploring the relationships between advisory safety speed and the dependent parameters like as traffic parameters(smooth condition and traffic jam), incident parameters(no-accident and accident) and road-surface parameters(dry, wet, snow). A simulation's results based on 12 scenarios show significant relationships and trends between 3 parameters and advisory safety speed. This model suggests that the advisory safety speed has more higher than average travel speed and is changeable by changing real-time incident states and road-surface states. The purpose of the research is to prove the new safety related services which are applicable in SMART Highway as traffic and IT convergence technology.

Intranasal Vaccination with Outer-Membrane Protein of Orientia tsutsugamushi induces Protective Immunity Against Scrub Typhus

  • Sung-Moo Park;Min Jeong Gu;Young-Jun Ju;In Su Cheon;Kyu-Jam Hwang;Byoungchul Gill;Byoung-Shik Shim;Hang-Jin Jeong;Young Min Son;Sangho Choi;Woonhee Jeung;Seung Hyun Han;Hyuk Chu;Cheol-Heui Yun
    • IMMUNE NETWORK
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    • v.21 no.2
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    • pp.14.1-14.17
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    • 2021
  • Scrub typhus develops after the individual is bitten by a trombiculid mite infected with Orientia tsutsugamushi. Since it has been reported that pneumonia is frequently observed in patients with scrub typhus, we investigated whether intranasal (i.n.) vaccination with the outer membrane protein of O. tsutsugamushi (OMPOT) would induce a protective immunity against O. tsutsugamushi infection. It was particular interest that when mice were infected with O. tsutsugamushi, the bacteria disseminated into the lungs, causing pneumonia. The i.n. vaccination with OMPOT induced IgG responses in serum and bronchoalveolar lavage (BAL) fluid. The anti-O. tsutsugamushi IgA Abs in BAL fluid after the vaccination showed a high correlation of the protection against O. tsutsugamushi. The vaccination induced strong Ag-specific Th1 and Th17 responses in the both spleen and lungs. In conclusion, the current study demonstrated that i.n. vaccination with OMPOT elicited protective immunity against scrub typhus in mouse with O. tsutsugamushi infection causing subsequent pneumonia.