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http://dx.doi.org/10.4163/jnh.2021.54.5.448

Effects of mulberry fruit juice powder on inflammation and microRNA-132/143 regulation in 3T3-L1 adipocytes  

Lee, Mak-Soon (Department of Nutritional Science and Food Management, Ewha Womans University)
Kim, Yangha (Department of Nutritional Science and Food Management, Ewha Womans University)
Publication Information
Journal of Nutrition and Health / v.54, no.5, 2021 , pp. 448-458 More about this Journal
Abstract
Purpose: Mulberry (Morus alba L.) fruit is widely grown in Asia and consumed as fresh fruit, jam, and juices. The fruit has beneficial health effects, including anti-diabetic, anti-tumor, and anti-obesity properties. However, the mechanisms by which mulberry fruit juice powder (MJ) regulates inflammatory microRNAs (miRs) are not yet known. This study investigated the effect of mulberry fruit juice powder on the regulation of inflammation and miR-132/143 during 3T3-L1 adipocyte differentiation. Methods: The 3T3-L1 cells were induced to differentiate for 2 days and then treated with various concentrations of MJ for 7 days. Cytotoxicity was determined by evaluating cell viability using a water-soluble tetrazolium salt-8 assay kit. Intracellular lipid accumulation was evaluated by oil-red O staining. The levels of the expression of genes involved in adipogenesis and inflammation, and miR-132/143 were measured by quantitative real-time polymerase chain reactions. Results: MJ showed no cytotoxic effect on 3T3-L1 adipocytes at concentrations below 100 ng/mL. Intracellular lipid accumulation was reduced by MJ treatment at concentrations of 100 ng/mL. The messenger RNA (mRNA) levels of proliferator-activated receptor-γ, cytosine-cytosine-adenosine-adenosine-thymidine/enhancer-binding protein-α, and adipocyte protein 2, which are involved in adipogenesis, were suppressed by MJ. A reduction was also seen in mRNA levels of genes related to the inflammatory response, such as tumor necrosis factor-α, interleukin-6, monocyte chemoattractant protein-1, and inducible nitric oxide synthase. The expression of the inflammatory miR-132 and miR-143 was also decreased by MJ. Conclusion: These results suggest that MJ may suppress adipogenesis and inflammation through the regulation of miR-132/143 expression in 3T3-L1 adipocytes. Thus, MJ may be useful as a food agent that prevents obesity-associated inflammation.
Keywords
Morus; fruit; inflammation; microRNAs; adipocytes;
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1 Tsuda T, Horio F, Osawa T. Cyanidin 3-O-beta-D-glucoside suppresses nitric oxide production during a zymosan treatment in rats. J Nutr Sci Vitaminol (Tokyo) 2002; 48(4): 305-310.   DOI
2 Strum JC, Johnson JH, Ward J, Xie H, Feild J, Hester A, et al. MicroRNA 132 regulates nutritional stress-induced chemokine production through repression of SirT1. Mol Endocrinol 2009; 23(11): 1876-1884.   DOI
3 Cook DG, Mendall MA, Whincup PH, Carey IM, Ballam L, Morris JE, et al. C-reactive protein concentration in children: relationship to adiposity and other cardiovascular risk factors. Atherosclerosis 2000; 149(1): 139-150.   DOI
4 Lee S, Lee MS, Chang E, Lee Y, Lee J, Kim J, et al. Mulberry fruit extract promotes serum HDL-cholesterol levels and suppresses hepatic microRNA-33 expression in rats fed high cholesterol/cholic acid diet. Nutrients 2020; 12(5): 1499.   DOI
5 Esau C, Kang X, Peralta E, Hanson E, Marcusson EG, Ravichandran LV, et al. MicroRNA-143 regulates adipocyte differentiation. J Biol Chem 2004; 279(50): 52361-52365.   DOI
6 Takanabe R, Ono K, Abe Y, Takaya T, Horie T, Wada H, et al. Up-regulated expression of microRNA-143 in association with obesity in adipose tissue of mice fed high-fat diet. Biochem Biophys Res Commun 2008; 376(4): 728-732.   DOI
7 Kay CD, Pereira-Caro G, Ludwig IA, Clifford MN, Crozier A. Anthocyanins and flavanones are more bioavailable than previously perceived: a review of recent evidence. Annu Rev Food Sci Technol 2017; 8: 155-180.   DOI
8 Kim HB, Park KJ, Seuk YS, Kim SL, Sung GB, Nam HW, et al. Morphological characteristics and physiological effects of mulberry leaves and fruits with wild varieties. Korean J Sericult Sci 2002; 44(1): 4-8.
9 Yuan Q, Zhao L. The mulberry (Morus alba L.) fruit-a review of characteristic components and health benefits. J Agric Food Chem 2017; 65(48): 10383-10394.   DOI
10 Lee MS, Kim Y. Mulberry fruit extract ameliorates adipogenesis via increasing AMPK activity and downregulating microRNA-21/143 in 3T3-L1 adipocytes. J Med Food 2020; 23(3): 266-272.   DOI
11 Jung S, Lee MS, Choi AJ, Kim CT, Kim Y. Anti-Inflammatory effects of high hydrostatic pressure extract of mulberry (Morus alba) fruit on LPS-stimulated RAW264.7 cells. Molecules 2019; 24(7): E1425.
12 Yang SJ, Park NY, Lim Y. Anti-adipogenic effect of mulberry leaf ethanol extract in 3T3-L1 adipocytes. Nutr Res Pract 2014; 8(6): 613-617.   DOI
13 Lee MS, Kim Y. Chrysanthemum morifolium flower extract inhibits adipogenesis of 3T3-L1 cells via AMPK/SIRT1 pathway activation. Nutrients 2020; 12(9): 2726.   DOI
14 Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCt method. Methods 2001; 25(4): 402-408.   DOI
15 Park HS, Shim SM, Kim GH. Inhibitory effects of ethyl acetate-soluble fraction from Morus alba on lipid accumulation in 3T3-L1 cells. Nat Prod Commun 2013; 8(11): 1579-1582.
16 Peng CH, Liu LK, Chuang CM, Chyau CC, Huang CN, Wang CJ. Mulberry water extracts possess an anti-obesity effect and ability to inhibit hepatic lipogenesis and promote lipolysis. J Agric Food Chem 2011; 59(6): 2663-2671.   DOI
17 Schoonjans K, Staels B, Auwerx J. The peroxisome proliferator activated receptors (PPARs) and their effects on lipid metabolism and adipocyte differentiation. Biochim Biophys Acta 1996; 1302(2): 93-109.   DOI
18 Storck M, Schilling M, Burkhardt K, Prestel R, Abendroth D, Hammer C. Production of proinflammatory cytokines and adhesion molecules in ex-vivo xenogeneic kidney perfusion. Transpl Int 1994; 7 Suppl 1: S647-S649.
19 Herath HM, Takano-Ishikawa Y, Yamaki K. Inhibitory effect of some flavonoids on tumor necrosis factor-alpha production in lipopolysaccharide-stimulated mouse macrophage cell line J774.1. J Med Food 2003; 6(4): 365-370.   DOI
20 Rosen ED, Hsu CH, Wang X, Sakai S, Freeman MW, Gonzalez FJ, et al. C/C/EBPα induces adipogenesis through PPARγ: a unified pathway. Genes Dev 2002; 16(1): 22-26.   DOI
21 Wink DA, Mitchell JB. Chemical biology of nitric oxide: Insights into regulatory, cytotoxic, and cytoprotective mechanisms of nitric oxide. Free Radic Biol Med 1998; 25(4-5): 434-456.   DOI
22 Jung S, Lee MS, Chang E, Kim CT, Kim Y. Mulberry (Morus alba L.) fruit extract ameliorates inflammation via regulating microRNA-21/132/143 expression and increases the skeletal muscle mitochondrial content and AMPK/SIRT activities. Antioxidants 2021; 10(9): 1453.   DOI
23 Kelley DS, Rasooly R, Jacob RA, Kader AA, Mackey BE. Consumption of Bing sweet cherries lowers circulating concentrations of inflammation markers in healthy men and women. J Nutr 2006; 136(4): 981-986.   DOI
24 Lim HH, Lee SO, Kim SY, Yang SJ, Lim Y. Anti-inflammatory and antiobesity effects of mulberry leaf and fruit extract on high fat diet-induced obesity. Exp Biol Med (Maywood) 2013; 238(10): 1160-1169.   DOI
25 O'Connell RM, Rao DS, Baltimore D. microRNA regulation of inflammatory responses. Annu Rev Immunol 2012; 30(1): 295-312.   DOI
26 Wang Y, Xiang L, Wang C, Tang C, He X. Antidiabetic and antioxidant effects and phytochemicals of mulberry fruit (Morus alba L.) polyphenol enhanced extract. PLoS One 2013; 8(7): e71144.   DOI
27 Alessi MC, Lijnen HR, Bastelica D, Juhan-Vague I. Adipose tissue and atherothrombosis. Pathophysiol Haemost Thromb 2003; 33(5-6): 290-297.   DOI
28 Shoelson SE, Herrero L, Naaz A. Obesity, inflammation, and insulin resistance. Gastroenterology 2007; 132(6): 2169-2180.   DOI
29 Barnes PJ, Karin M. Nuclear factor-kappaB: a pivotal transcription factor in chronic inflammatory diseases. N Engl J Med 1997; 336(15): 1066-1071.   DOI
30 Folin O, Denis W. A colorimetric method for the determination of phenols (and phenol derivatives) in urine. J Biol Chem 1915; 22(2): 305-308.   DOI
31 Choi JW, Synytsya A, Capek P, Bleha R, Pohl R, Park YI. Structural analysis and anti-obesity effect of a pectic polysaccharide isolated from Korean mulberry fruit Oddi (Morus alba L.). Carbohydr Polym 2016; 146: 187-196.   DOI
32 Charriere G, Cousin B, Arnaud E, Andre M, Bacou F, Penicaud L, et al. Preadipocyte conversion to macrophage. Evidence of plasticity. J Biol Chem 2003; 278(11): 9850-9855.   DOI
33 Zdravkovic JM, Acamovic-Djokovic GS, Mladenovic JD, Pavlovic RM, Zdravkovic MS. Antioxidant capacity and contents of phenols, ascorbic acid, β-carotene and lycopene in lettuce. Hem Ind 2014; 68(2): 193-198.   DOI