• Title/Summary/Keyword: isometric sequences

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RT-PCR Detection of dsRNA Mycoviruses Infecting Pleurotus ostreatus and Agaricus blazei Murrill

  • Kim, Yu-Jeong;Park, Sang-Ho;Yie, Se-Won;Kim, Kook-Hyung
    • The Plant Pathology Journal
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    • v.21 no.4
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    • pp.343-348
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    • 2005
  • The partial nucleotide sequences of the genomic dsRNA mycoviruses infecting Pleurotus ostreatus (isolates ASI2596, ASI2597, and Bupyungbokhoe) and Agaricus blazei Murrill were determined and compared with those of the other dsRNA mycoviruses. Partial nucleotide sequences of the purified dsRNA from ASI2596 and ASI2597 revealed RNA-dependent RNA polymerase sequences that are closely related to Oyster mushroom isometric virus 2, while nucleotide sequences and the deduced amino acid sequence from dsRNA mycovirus infecting Agaricus blazei did not show any significant homology to the other dsRNA mycoviruses. Specific primers were designed for RT-PCR detection of these dsRNA viruses and were found to specifically detect each dsRNA virus. Northern blot analysis confirmed the homogeneity of RT-PCR products to each purified dsRNA. Altogether, our results suggest that these virus-specific primer sets can be employed for the specific detection of each dsRNA mycovirus in infected mushrooms.

Characterization and RT-PCR Detection of dsRNA Mycoviruses from the Oyster Mushroom, Pleurotus ostreatus

  • Seo, Jang-Kyun;Lim, Won-Seok;Jeong, Ji-Hye;Yoo, Young-Bok;Yie, Se-Won;Kim, Kook-Hyung
    • The Plant Pathology Journal
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    • v.20 no.3
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    • pp.200-205
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    • 2004
  • The partial nucleotide sequences of the genomic dsRNA mycovirus infecting Pleurotus ostreatus isolates ASI2223 and Suhan were determined and compared with those of mycoviruses belonging to partitiviruses and totiviruses. Partial nucleotide sequences of the purified dsRNA from ASI2223 and Suhan showed RNA-dependent RNA polymerase sequences that are closely related to those of partitiviruses, including Fusarium poae virus 1, Fusarium solani virus, Rhizoctoniasolani virus, Discula destructiva virus 2, and Oyster mushroom isometric virus 2. Specific primers were designed for RT-PCR detection of dsRNA viruses from the P. ostreatus isolate ASI2223 and Suhan. Two virus specific primer sets were found to specifically detect each virus among six sets of designed oligonucleotide primers. Collectively, these results suggest that dsRNA mycoviruses from P. ostreatus isolates ASI2223 and Suhan belong to the family Partitiviridae, although, they are not the same virus species. Our results also suggest that these virus-specific primer sets can be employed for the specific detection of each viral sequence in infected tissues.

PLANK PROBLEMS, POLARIZATION AND CHEBYSHEV CONSTANTS

  • Revesz, Szilard-Gy.;Sarantopoulos, Yannis
    • Journal of the Korean Mathematical Society
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    • v.41 no.1
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    • pp.157-174
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    • 2004
  • In this work we discuss "plank problems" for complex Banach spaces and in particular for the classical $L^{p}(\mu)$ spaces. In the case $1\;{\leq}\;p\;{\leq}\;2$ we obtain optimal results and for finite dimensional complex Banach spaces, in a special case, we have improved an early result by K. Ball [3]. By using these results, in some cases we are able to find best possible lower bounds for the norms of homogeneous polynomials which are products of linear forms. In particular, we give an estimate in the case of a real Hilbert space which seems to be a difficult problem. We have also obtained some results on the so-called n-th (linear) polarization constant of a Banach space which is an isometric property of the space. Finally, known polynomial inequalities have been derived as simple consequences of various results related to plank problems.

CHARACTERIZATION OF FINITE COLORED SPACES WITH CERTAIN CONDITIONS

  • Hirasaka, Mitsugu;Shinohara, Masashi
    • Journal of the Korean Mathematical Society
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    • v.56 no.3
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    • pp.579-594
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    • 2019
  • A colored space is a pair (X, r) of a set X and a function r whose domain is $\(^X_2\)$. Let (X, r) be a finite colored space and $Y,\;Z{\subseteq}X$. We shall write $Y{\simeq}_rZ$ if there exists a bijection $f:Y{\rightarrow}Z$ such that r(U) = r(f(U)) for each $U{\in}\({^Y_2}\)$ where $f(U)=\{f(u){\mid}u{\in}U\}$. We denote the numbers of equivalence classes with respect to ${\simeq}_r$ contained in $\(^X_i\)$ by $a_i(r)$. In this paper we prove that $a_2(r){\leq}a_3(r)$ when $5{\leq}{\mid}X{\mid}$, and show what happens when equality holds.

Characterization of Prunus necrotic ringspot virus Isolate from Peach in Korea (국내 복숭아에서 분리한 Prunus necrotic ringspot virus의 특성)

  • Kim, Hyun-Ran;Lee, Sin-Ho;Shin, Il-Sheob;Kim, Jeong-Hee;Cho, Kang-Hee;Heo, Seong;Kim, Jeong-Soo;Choi, Yong-Mun
    • Research in Plant Disease
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    • v.15 no.3
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    • pp.170-174
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    • 2009
  • In this paper, we report a characterization of Prunus necrotic ringspot virus (PNRSV) isolate. The virus was identified from 'Yumyeong' peach showing mild mosaic on leaves in commercial orchard of 'Umsung', Chungbuk province in Korea. The virus isolate produced ringspot symptom on the inoculated cotyledons and systemic mosaic and malformation on the upper leaves of Cucumis sativus. Systemic mottles were appeared in Chenopodium quinoa. When the buds of the virus infected stem were grafted on the healthy young Prunus persica GF305 seedlings, line pattern with mosaic appeared within 3 months. Isometric virus-like particles were found in parenchyma cells and plasmodesmata of C. sativus leaves inoculated mechanically with the virus. The cDNA fragments of PNRSV coat protein (CP) region, approximately 675bp, were synthesized from genomic RNA extracted from virus-infected leaves by RT-PCR using specific primer pairs. Partial nucleotide sequences of the CP regions were determined and analyzed with the known PNRSV. The CP gene of PNRSVKorea isolates showed 93.9~94.7% similarity to the 4 known PNRSV isolates.