DOI QR코드

DOI QR Code

Characterization and RT-PCR Detection of dsRNA Mycoviruses from the Oyster Mushroom, Pleurotus ostreatus

  • Seo, Jang-Kyun (School of Agricultural Biotechnology and Center for Plant Molecular Genetics and Breeding Research, Seoul National University) ;
  • Lim, Won-Seok (School of Agricultural Biotechnology and Center for Plant Molecular Genetics and Breeding Research, Seoul National University) ;
  • Jeong, Ji-Hye (Division of Life Sciences, College of Natural Sciences, Kangwon National University) ;
  • Yoo, Young-Bok (National Institute of Agricultural Science and Technology, Rural Development Administration) ;
  • Yie, Se-Won (Division of Life Sciences, College of Natural Sciences, Kangwon National University) ;
  • Kim, Kook-Hyung (School of Agricultural Biotechnology and Center for Plant Molecular Genetics and Breeding Research, Seoul National University)
  • Published : 2004.09.01

Abstract

The partial nucleotide sequences of the genomic dsRNA mycovirus infecting Pleurotus ostreatus isolates ASI2223 and Suhan were determined and compared with those of mycoviruses belonging to partitiviruses and totiviruses. Partial nucleotide sequences of the purified dsRNA from ASI2223 and Suhan showed RNA-dependent RNA polymerase sequences that are closely related to those of partitiviruses, including Fusarium poae virus 1, Fusarium solani virus, Rhizoctoniasolani virus, Discula destructiva virus 2, and Oyster mushroom isometric virus 2. Specific primers were designed for RT-PCR detection of dsRNA viruses from the P. ostreatus isolate ASI2223 and Suhan. Two virus specific primer sets were found to specifically detect each virus among six sets of designed oligonucleotide primers. Collectively, these results suggest that dsRNA mycoviruses from P. ostreatus isolates ASI2223 and Suhan belong to the family Partitiviridae, although, they are not the same virus species. Our results also suggest that these virus-specific primer sets can be employed for the specific detection of each viral sequence in infected tissues.

Keywords

References

  1. Boland, G. J. 1992. Hypovirulence and double-stranded RNA in Sclerotinia sclerotiorum. Can. J. Plant Pathol. 14:10-17 https://doi.org/10.1080/07060669209500900
  2. Chen, B., Choi, G. H. and Nuss, D. L. 1994. Attenuation of fungal virulence by synthetic infectious hypovirus transcripts. Science 264:1762-1764 https://doi.org/10.1126/science.8209256
  3. Cheng, J., Jiang, D., Fu, Y., Li, G., Peng, Y. and Ghabrial, S. A. 2003. Molecular characterization of a dsRNA totivirus infecting the sclerotial parasite Coniothyrium minitans. Virus Res. 93:41-50 https://doi.org/10.1016/S0168-1702(03)00064-9
  4. Choi, G. H. and Nuss, D. L. 1992. A viral gene confers hypovirulence associated traits to the chestnut blight fungus. EMBO J. 11:473-477
  5. Chu, Y.-M., Jeon, J.-J., Yea, S.-J., Kim, Y.-H., Yun, S.-H., Lee, Y.-W. and Kim, K.-H. 2002. Double-stranded RNA mycovirus from Fusarium graminearum. Appl. Environ. Microbiol. 68:2529-2534 https://doi.org/10.1128/AEM.68.5.2529-2534.2002
  6. Chu, Y.-M., Lim, W-S., Yea, S.-J., Cho, J.-D., Lee, Y.-W. and Kim, K.-H. 2004. Complexity of dsRNA mycovirus isolated from Fusarium graminearum. Virus Genes 28:135-143 https://doi.org/10.1023/B:VIRU.0000012270.67302.35
  7. Cole, T. E., Muller, B., Hong, Y., Brasier, C. M. and Buck, K. W. 1998. Complexity of virus-like double-stranded RNA elements in a diseased isolate of the Dutch elm disease fungus, Ophiostoma novo-ulmi. J. Phytopathol. 146:593-598 https://doi.org/10.1111/j.1439-0434.1998.tb04760.x
  8. Compel, P., Papp, I., Bibo, M., Fekete, C. and Hornok, L. 1999. Genetic interrelationships and genome organization of doublestranded RNA elements of Fusarium poae. Virus Genes 18:49-56 https://doi.org/10.1023/A:1008069318838
  9. Dieleman-Van Zaayen, A. 1969. A virus disease of cultivated mushroom in the Netherlands. Neth. J. PI. Pathol. 74:48-51
  10. Dieleman-Van Zaayen, A. 1972. Intracellular appearance of mushroom virus in fruiting bodies and basidiospores of Agaricus blsporus. Virology 47:94-104 https://doi.org/10.1016/0042-6822(72)90242-5
  11. Ghabrial, S. A. 1986. A transmissible disease of Helminthosporium victoriae evidence for a viral etiology. pp. 164-176. In: Buck, K.W. ed. Fungal VIrology. CRC Press, Boca Raton
  12. Go, S. J., Cha, D. Y., Shin, G. C. and Wessels, J. G. H. 1992. Molecular analysis of double-stranded RNA in abnormal growing Oyster-mushrooms, Pleurotus florida and P. ostreatus due to Virus Injection. Kor. Mycol. 20:234-239
  13. Goodin, M. M., Schlagnhaufer, B. and Romaine, C. P. 1992. Encapsidation of the La France disease-specific doublestranded RNA's in 36 nm isometric viruslike particles. Phytopathology 82:285-290 https://doi.org/10.1094/Phyto-82-285
  14. Goodin, M. M., Schlagnhaufer, B., Weir, T. and Romaine, C. P. 1997. Characterization of an RNA-dependent RNA polymerase activity associated with La France isometric virus. J. Virol. 71 :2264-2269
  15. Gubler, U. and Hoffman, B. J. 1983. A simple and very efficient method for generating cNA libraries. Gene 25:163-269
  16. Hammar, S., Fulbright, D. W. and Adams, G. C. 1989. Association of double-stranded RNA with low virulence in an isolate of Leucostoma persoonii. Phytopathology 79:568-572 https://doi.org/10.1094/Phyto-79-568
  17. Hicks, R. T. G. and Haughton, K. L. 1986. Detection of doublestranded RNA in shake-cultures of Agaricus bisporus affected by La France disease. Trans. Br. Mycol. Soc. 86:578-584
  18. Hillman, B. I., Tian, Y., Bedker, P. J. and Brown, M. P. 1992. A North American hypovirulent isolate of the chestnut blight fungus with European isolate-related dsRNA J. Gen. Virol. 73:681-686 https://doi.org/10.1099/0022-1317-73-3-681
  19. Hollings, M. 1962. Viruses associated with dieback disease of cultivated mushrooms. Nature 196:962-965 https://doi.org/10.1038/196962a0
  20. Jian,J. H., Lakshman, D. K. and Tavantzis, S. M. 1998. A virulence- associated, 6.4-kb, double-stranded RNA from Rhizoctonia solani is phylogenetically related to plant bromoviruses and electron transport enzymes. Mol. Plant Microbe Interact. 11:601-609 https://doi.org/10.1094/MPMI.1998.11.7.601
  21. Koonin, E. V. 1991. The phylogeny of RNA-dependent RNA polymerases of positive-strand RNA viruses. J. Gen. Virol. 72:2197-2206 https://doi.org/10.1099/0022-1317-72-9-2197
  22. Koonin, E. V. and Dolja, V. V. 1993. Evolution and taxonomy of positive-strand RNA viruses: implications of comparative analysis of amino acid sequences. Crit. Rev. Biochem. Mol. BioI. 28:375-430 https://doi.org/10.3109/10409239309078440
  23. Koons, K., Schlagnhaufer, B. and Romaine, C. P. 1989. Double stranded RNAs in mycelial cultures of Agaricus bisporus affected by La France disease. Phytopathology 79: 1272-1275 https://doi.org/10.1094/Phyto-79-1272
  24. Lee, H.-S. 2000. Detection of three isometric viruses in oyster mushroom, Pleurotus ostreatus. Kor. Soc. Mycol. News Lett. 12:5-6
  25. Lemke, P. A 1979. Viruses and plasmids in fungi. Marcel Dekker, New York. p. 653
  26. Liang, P., Liu, H. and Chen, K. 1990. Intracellular appearance, morphological features and properties of oyster mushroom virus. Mycol. Res 94:529-537 https://doi.org/10.1016/S0953-7562(10)80016-4
  27. Preisig, O., Moleleki, N., Smit, W. A., Wingfield, B. D. and Wingfield, M. J. 2000. A novel RNA mycovirus in a hypovirulent isolate of the plant pathogen Diaporthe ambigua. J. Gen. Virol. 81:3107-3114 https://doi.org/10.1099/0022-1317-81-12-3107
  28. Revill, P. A. and Wright, P. J. 1997. RT-PCR detection of dsRNAs associated with La France disease of the cultivated mushroom Agaricus bisporus (Lange) Imbach. J. Virol. Methods 63:17-26 https://doi.org/10.1016/S0166-0934(96)02113-1
  29. Ross, R. C., Brown, G. A. and Romaine, C. P. 1986. Recent experience in detecting viral double-stranded RNA in commercial mushroom crops and its effect on yield. pp. 321-329. In: Wuest, P.J., Royse, D. J. and Beelman, R. B. eds. Cultivating Edible Fungi. Elsevier, Amsterdam
  30. Schisler, L. C., Sinden, J. W. and Sigel, E. M. 1967. Etiology, symptomatology, and epidemiology of a virus disease of cultivated mushrooms. Phytopathology 57:519-526
  31. Sinden, J. W. and Hauser, E. 1950. Report on two new mushroom diseases. Mush. Sci. 1:96-100
  32. Thompson, J. D., Higgins, D. G. and Gibson, T. J. 1994. CLUSTALW: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680 https://doi.org/10.1093/nar/22.22.4673
  33. van der Lende, T. R., Harmsen, M. C. and Wessels, J. G. H. 1994. Double-stranded RNAs and proteins associated with the 34 nm virus particles of the cultivated mushroom Agaricus bisporus. J. Gen. Virol. 75:2533-2536 https://doi.org/10.1099/0022-1317-75-9-2533
  34. van der Lende, T. R., Harmsen, M. C., Go, S. J. and Wessels, J. G. H. 1995. Double-stranded RNA mycoviruses in mycelium of Pleurotus ostreatus. FEMS Micorbiol. Lett. 125:51-56
  35. van der Lende, T. R., Duitman, E. H., Gunnewijk, M. G., Yu, L. and Wessels, J. G. H. 1996. Functional analysis of dsRNAs (L1, L3, L5, and M2) associated with isomatric 34 nm virions of Agaricus bisporus (white button mushroom). Virology 217:88-96 https://doi.org/10.1006/viro.1996.0096
  36. Wach, M. P., Sriskantha, A. and Romaine, C. P. 1987. Doublestranded RNAs associated with La France disease of the commercial mushroom. Phytopathology 77: 1321-1325 https://doi.org/10.1094/Phyto-77-1321
  37. Yu, H. J., Lim, D. and Lee, H.-S. 2003. Characterization of a novel single-stranded RNA mycovirus in Pleuratus ostreatus. Virology 314:9-15 https://doi.org/10.1016/S0042-6822(03)00382-9
  38. Zhang, J. and Madden, T. L. 1997. Powe rBLAST: A new network BLAST application for interactive or automated sequence analysis and annotation. Genome Res. 7:649-656 https://doi.org/10.1101/gr.7.6.649

Cited by

  1. A New Double-Stranded RNA Mycovirus from Pleurotus ostreatus (ASI 2504) vol.22, pp.1, 2006, https://doi.org/10.5423/PPJ.2006.22.1.068
  2. Taxonomy, biogeography and importance of Heterobasidion viruses vol.219, 2016, https://doi.org/10.1016/j.virusres.2015.10.014
  3. Two viruses of Heterobasidion confer beneficial, cryptic or detrimental effects to their hosts in different situations vol.6, pp.5, 2013, https://doi.org/10.1016/j.funeco.2013.05.005
  4. The identification of a novel Pleurotus ostreatus dsRNA virus and determination of the distribution of viruses in mushroom spores vol.46, pp.1, 2008, https://doi.org/10.1007/s12275-007-0171-y