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http://dx.doi.org/10.5423/RPD.2009.15.3.170

Characterization of Prunus necrotic ringspot virus Isolate from Peach in Korea  

Kim, Hyun-Ran (Fruit Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration)
Lee, Sin-Ho (Korea Fruit Agricultural Co.)
Shin, Il-Sheob (Fruit Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration)
Kim, Jeong-Hee (Fruit Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration)
Cho, Kang-Hee (Fruit Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration)
Heo, Seong (Fruit Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration)
Kim, Jeong-Soo (National Academy of Agricultural Science, R.D.A.)
Choi, Yong-Mun (Rural Development Administration)
Publication Information
Research in Plant Disease / v.15, no.3, 2009 , pp. 170-174 More about this Journal
Abstract
In this paper, we report a characterization of Prunus necrotic ringspot virus (PNRSV) isolate. The virus was identified from 'Yumyeong' peach showing mild mosaic on leaves in commercial orchard of 'Umsung', Chungbuk province in Korea. The virus isolate produced ringspot symptom on the inoculated cotyledons and systemic mosaic and malformation on the upper leaves of Cucumis sativus. Systemic mottles were appeared in Chenopodium quinoa. When the buds of the virus infected stem were grafted on the healthy young Prunus persica GF305 seedlings, line pattern with mosaic appeared within 3 months. Isometric virus-like particles were found in parenchyma cells and plasmodesmata of C. sativus leaves inoculated mechanically with the virus. The cDNA fragments of PNRSV coat protein (CP) region, approximately 675bp, were synthesized from genomic RNA extracted from virus-infected leaves by RT-PCR using specific primer pairs. Partial nucleotide sequences of the CP regions were determined and analyzed with the known PNRSV. The CP gene of PNRSVKorea isolates showed 93.9~94.7% similarity to the 4 known PNRSV isolates.
Keywords
Ilarvirus; Indicator assay; RT-PCR detection; Peach; PNRSV;
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Times Cited By KSCI : 2  (Citation Analysis)
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