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http://dx.doi.org/10.5423/PPJ.2004.20.3.200

Characterization and RT-PCR Detection of dsRNA Mycoviruses from the Oyster Mushroom, Pleurotus ostreatus  

Seo, Jang-Kyun (School of Agricultural Biotechnology and Center for Plant Molecular Genetics and Breeding Research, Seoul National University)
Lim, Won-Seok (School of Agricultural Biotechnology and Center for Plant Molecular Genetics and Breeding Research, Seoul National University)
Jeong, Ji-Hye (Division of Life Sciences, College of Natural Sciences, Kangwon National University)
Yoo, Young-Bok (National Institute of Agricultural Science and Technology, Rural Development Administration)
Yie, Se-Won (Division of Life Sciences, College of Natural Sciences, Kangwon National University)
Kim, Kook-Hyung (School of Agricultural Biotechnology and Center for Plant Molecular Genetics and Breeding Research, Seoul National University)
Publication Information
The Plant Pathology Journal / v.20, no.3, 2004 , pp. 200-205 More about this Journal
Abstract
The partial nucleotide sequences of the genomic dsRNA mycovirus infecting Pleurotus ostreatus isolates ASI2223 and Suhan were determined and compared with those of mycoviruses belonging to partitiviruses and totiviruses. Partial nucleotide sequences of the purified dsRNA from ASI2223 and Suhan showed RNA-dependent RNA polymerase sequences that are closely related to those of partitiviruses, including Fusarium poae virus 1, Fusarium solani virus, Rhizoctoniasolani virus, Discula destructiva virus 2, and Oyster mushroom isometric virus 2. Specific primers were designed for RT-PCR detection of dsRNA viruses from the P. ostreatus isolate ASI2223 and Suhan. Two virus specific primer sets were found to specifically detect each virus among six sets of designed oligonucleotide primers. Collectively, these results suggest that dsRNA mycoviruses from P. ostreatus isolates ASI2223 and Suhan belong to the family Partitiviridae, although, they are not the same virus species. Our results also suggest that these virus-specific primer sets can be employed for the specific detection of each viral sequence in infected tissues.
Keywords
sRNA mycovirus; partitivirus; Pleurotus ostreatus; RT-PCR detection;
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1 Chu, Y.-M., Jeon, J.-J., Yea, S.-J., Kim, Y.-H., Yun, S.-H., Lee, Y.-W. and Kim, K.-H. 2002. Double-stranded RNA mycovirus from Fusarium graminearum. Appl. Environ. Microbiol. 68:2529-2534   DOI   PUBMED
2 Dieleman-Van Zaayen, A. 1969. A virus disease of cultivated mushroom in the Netherlands. Neth. J. PI. Pathol. 74:48-51
3 Ghabrial, S. A. 1986. A transmissible disease of Helminthosporium victoriae evidence for a viral etiology. pp. 164-176. In: Buck, K.W. ed. Fungal VIrology. CRC Press, Boca Raton
4 Go, S. J., Cha, D. Y., Shin, G. C. and Wessels, J. G. H. 1992. Molecular analysis of double-stranded RNA in abnormal growing Oyster-mushrooms, Pleurotus florida and P. ostreatus due to Virus Injection. Kor. Mycol. 20:234-239
5 Hollings, M. 1962. Viruses associated with dieback disease of cultivated mushrooms. Nature 196:962-965   DOI
6 Jian,J. H., Lakshman, D. K. and Tavantzis, S. M. 1998. A virulence- associated, 6.4-kb, double-stranded RNA from Rhizoctonia solani is phylogenetically related to plant bromoviruses and electron transport enzymes. Mol. Plant Microbe Interact. 11:601-609   DOI   ScienceOn
7 Preisig, O., Moleleki, N., Smit, W. A., Wingfield, B. D. and Wingfield, M. J. 2000. A novel RNA mycovirus in a hypovirulent isolate of the plant pathogen Diaporthe ambigua. J. Gen. Virol. 81:3107-3114   DOI
8 Ross, R. C., Brown, G. A. and Romaine, C. P. 1986. Recent experience in detecting viral double-stranded RNA in commercial mushroom crops and its effect on yield. pp. 321-329. In: Wuest, P.J., Royse, D. J. and Beelman, R. B. eds. Cultivating Edible Fungi. Elsevier, Amsterdam
9 Thompson, J. D., Higgins, D. G. and Gibson, T. J. 1994. CLUSTALW: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680   DOI   ScienceOn
10 van der Lende, T. R., Duitman, E. H., Gunnewijk, M. G., Yu, L. and Wessels, J. G. H. 1996. Functional analysis of dsRNAs (L1, L3, L5, and M2) associated with isomatric 34 nm virions of Agaricus bisporus (white button mushroom). Virology 217:88-96   DOI   ScienceOn
11 Wach, M. P., Sriskantha, A. and Romaine, C. P. 1987. Doublestranded RNAs associated with La France disease of the commercial mushroom. Phytopathology 77: 1321-1325   DOI
12 Hammar, S., Fulbright, D. W. and Adams, G. C. 1989. Association of double-stranded RNA with low virulence in an isolate of Leucostoma persoonii. Phytopathology 79:568-572   DOI
13 Zhang, J. and Madden, T. L. 1997. Powe rBLAST: A new network BLAST application for interactive or automated sequence analysis and annotation. Genome Res. 7:649-656   DOI
14 Hicks, R. T. G. and Haughton, K. L. 1986. Detection of doublestranded RNA in shake-cultures of Agaricus bisporus affected by La France disease. Trans. Br. Mycol. Soc. 86:578-584
15 Koonin, E. V. 1991. The phylogeny of RNA-dependent RNA polymerases of positive-strand RNA viruses. J. Gen. Virol. 72:2197-2206   DOI   ScienceOn
16 Koons, K., Schlagnhaufer, B. and Romaine, C. P. 1989. Double stranded RNAs in mycelial cultures of Agaricus bisporus affected by La France disease. Phytopathology 79: 1272-1275   DOI
17 Yu, H. J., Lim, D. and Lee, H.-S. 2003. Characterization of a novel single-stranded RNA mycovirus in Pleuratus ostreatus. Virology 314:9-15   DOI   ScienceOn
18 Boland, G. J. 1992. Hypovirulence and double-stranded RNA in Sclerotinia sclerotiorum. Can. J. Plant Pathol. 14:10-17   DOI
19 Dieleman-Van Zaayen, A. 1972. Intracellular appearance of mushroom virus in fruiting bodies and basidiospores of Agaricus blsporus. Virology 47:94-104   DOI   PUBMED   ScienceOn
20 Cheng, J., Jiang, D., Fu, Y., Li, G., Peng, Y. and Ghabrial, S. A. 2003. Molecular characterization of a dsRNA totivirus infecting the sclerotial parasite Coniothyrium minitans. Virus Res. 93:41-50   DOI   ScienceOn
21 Cole, T. E., Muller, B., Hong, Y., Brasier, C. M. and Buck, K. W. 1998. Complexity of virus-like double-stranded RNA elements in a diseased isolate of the Dutch elm disease fungus, Ophiostoma novo-ulmi. J. Phytopathol. 146:593-598   DOI   ScienceOn
22 Lee, H.-S. 2000. Detection of three isometric viruses in oyster mushroom, Pleurotus ostreatus. Kor. Soc. Mycol. News Lett. 12:5-6
23 van der Lende, T. R., Harmsen, M. C. and Wessels, J. G. H. 1994. Double-stranded RNAs and proteins associated with the 34 nm virus particles of the cultivated mushroom Agaricus bisporus. J. Gen. Virol. 75:2533-2536   DOI   ScienceOn
24 Sinden, J. W. and Hauser, E. 1950. Report on two new mushroom diseases. Mush. Sci. 1:96-100
25 Koonin, E. V. and Dolja, V. V. 1993. Evolution and taxonomy of positive-strand RNA viruses: implications of comparative analysis of amino acid sequences. Crit. Rev. Biochem. Mol. BioI. 28:375-430   DOI   ScienceOn
26 van der Lende, T. R., Harmsen, M. C., Go, S. J. and Wessels, J. G. H. 1995. Double-stranded RNA mycoviruses in mycelium of Pleurotus ostreatus. FEMS Micorbiol. Lett. 125:51-56
27 Schisler, L. C., Sinden, J. W. and Sigel, E. M. 1967. Etiology, symptomatology, and epidemiology of a virus disease of cultivated mushrooms. Phytopathology 57:519-526
28 Gubler, U. and Hoffman, B. J. 1983. A simple and very efficient method for generating cNA libraries. Gene 25:163-269
29 Lemke, P. A 1979. Viruses and plasmids in fungi. Marcel Dekker, New York. p. 653
30 Revill, P. A. and Wright, P. J. 1997. RT-PCR detection of dsRNAs associated with La France disease of the cultivated mushroom Agaricus bisporus (Lange) Imbach. J. Virol. Methods 63:17-26   DOI   PUBMED   ScienceOn
31 Liang, P., Liu, H. and Chen, K. 1990. Intracellular appearance, morphological features and properties of oyster mushroom virus. Mycol. Res 94:529-537   DOI
32 Goodin, M. M., Schlagnhaufer, B., Weir, T. and Romaine, C. P. 1997. Characterization of an RNA-dependent RNA polymerase activity associated with La France isometric virus. J. Virol. 71 :2264-2269
33 Hillman, B. I., Tian, Y., Bedker, P. J. and Brown, M. P. 1992. A North American hypovirulent isolate of the chestnut blight fungus with European isolate-related dsRNA J. Gen. Virol. 73:681-686   DOI   ScienceOn
34 Choi, G. H. and Nuss, D. L. 1992. A viral gene confers hypovirulence associated traits to the chestnut blight fungus. EMBO J. 11:473-477
35 Chu, Y.-M., Lim, W-S., Yea, S.-J., Cho, J.-D., Lee, Y.-W. and Kim, K.-H. 2004. Complexity of dsRNA mycovirus isolated from Fusarium graminearum. Virus Genes 28:135-143   DOI   ScienceOn
36 Chen, B., Choi, G. H. and Nuss, D. L. 1994. Attenuation of fungal virulence by synthetic infectious hypovirus transcripts. Science 264:1762-1764   DOI   PUBMED
37 Goodin, M. M., Schlagnhaufer, B. and Romaine, C. P. 1992. Encapsidation of the La France disease-specific doublestranded RNA's in 36 nm isometric viruslike particles. Phytopathology 82:285-290   DOI
38 Compel, P., Papp, I., Bibo, M., Fekete, C. and Hornok, L. 1999. Genetic interrelationships and genome organization of doublestranded RNA elements of Fusarium poae. Virus Genes 18:49-56   DOI   ScienceOn