• Title/Summary/Keyword: isolation, identification

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Design and Analysis of an Active Vibration Isolation System (능동형 제진 시스템의 설계 및 해석)

  • Moon, Jun-Hee;Pahk, Heui-Jae
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 2004.11a
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    • pp.647-650
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    • 2004
  • The modeling of an active vibration isolation system is accomplished by using the equivalent spring constant, mass and rotational Inertia of each component. The detailed model of the actuation module is successful for describing its frequency-domain performance but also too complicated to implement it to actual system for control so that the order of the model is reduced up to the degree that preserves its characteristic in the low frequency range. The reduced model is suitable for identifying the unknown system parameters such as damping constants of components. The overall isolation system is described by using the reduced model of the actuation module. The accurate model ing and system parameter identification that is essential for the control of the active vibration isolation system is attained successfully.

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Isolation of Environmental Mycobacteria from Diverse Water Samples Using Cetylpyridinium Chloride

  • Choi, Yeon-Im;Jin, Hyun-Woo;Lee, Gyu-Sang;Kim, Jong-Bae;Song, In-Kenn;Kim, Young-Joon;Lee, Hye-Young
    • Biomedical Science Letters
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    • v.16 no.3
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    • pp.133-138
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    • 2010
  • Despite of the increasing importance of environmental mycobacteria, detection and identification of mycobacteria from environmental sources including water have been fraught with technical difficulties. Although, several protocols to optiruize isolation of mycobacteria from water sources have been reported, standard method has not yet been established. In this study, usefulness of cetylpyridinium chloride (CPC), a cationic quaternary ammonium compound, for the isolation of environmental mycobacteria from diverse water samples was evaluated. For this, water samples from diverse water sources such as effluent water, lake water, and underground water were collected, treated with diverse concentrations of CPC, and plated on the solid agar plates. Subsequently individual colonies grown on the plates were sequence analyzed for identification of each colony. In brief, the results from this study showed that the growth of mycobacteria was enhanced by use of CPC as a pre-treatment reagent to water samples by inhibiting growth of other non-mycobacteria in water. In fact, the effect of CPC to decontaminate non-mycobacteria for isolation of mycobacteria was better than 1~4% of NaOH, which is a routinely used decontaminating reagent widely employed for culturing mycobactera from sputum specimens. Therefore, the results from this study seems to support that the CPC pre-treatment may be useful for isolation of mycobacteria from diverse sources including clinical specimens which are often contaminated with other bacteria.

A New Isolation and Evaluation Method for Marine-Derived Yeast spp. with Potential Applications in Industrial Biotechnology

  • Zaky, Abdelrahman Saleh;Greetham, Darren;Louis, Edward J.;Tucker, Greg A.;Du, Chenyu
    • Journal of Microbiology and Biotechnology
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    • v.26 no.11
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    • pp.1891-1907
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    • 2016
  • Yeasts that are present in marine environments have evolved to survive hostile environments that are characterized by high exogenous salt content, high concentrations of inhibitory compounds, and low soluble carbon and nitrogen levels. Therefore, yeasts isolated from marine environments could have interesting characteristics for industrial applications. However, the application of marine yeast in research or industry is currently very limited owing to the lack of a suitable isolation method. Current methods for isolation suffer from fungal interference and/or low number of yeast isolates. In this paper, an efficient and non-laborious isolation method has been developed and successfully isolated large numbers of yeasts without bacterial or fungal growth. The new method includes a three-cycle enrichment step followed by an isolation step and a confirmation step. Using this method, 116 marine yeast strains were isolated from 14 marine samples collected in the UK, Egypt, and the USA. These strains were further evaluated for the utilization of fermentable sugars (glucose, xylose, mannitol, and galactose) using a phenotypic microarray assay. Seventeen strains with higher sugar utilization capacity than the reference terrestrial yeast Saccharomyces cerevisiae NCYC 2592 were selected for identification by sequencing of the ITS and D1/D2 domains. These strains belonged to six species: S. cerevisiae, Candida tropicalis, Candida viswanathii, Wickerhamomyces anomalus, Candida glabrata, and Pichia kudriavzevii. The ability of these strains for improved sugar utilization using seawater-based media was confirmed and, therefore, they could potentially be utilized in fermentations using marine biomass in seawater media, particularly for the production of bioethanol and other biochemical products.

Base isolated RC building - performance evaluation and numerical model updating using recorded earthquake response

  • Nath, Rupam Jyoti;Deb, Sajal Kanti;Dutta, Anjan
    • Earthquakes and Structures
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    • v.4 no.5
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    • pp.471-487
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    • 2013
  • Performance of a prototype base isolated building located at Indian Institute of Technology, Guwahati (IITG) has been studied here. Two numbers of three storeyed single bay RCC framed prototype buildings were constructed for experimental purpose at IITG, one supported on conventional isolated footings and the other on a seismic isolation system, consisting of lead plug bearings. Force balance accelerometers and a 12 channel strong motion recorder have been used for recording building response during seismic events. Floor responses from these buildings show amplification for the conventional building while 60 to 70% reduction has been observed for the isolated building. Numerical models of both the buildings have been created in SAP2000 Nonlinear. Infill walls have been modeled as compression struts and have been incorporated into the 3D models using Gap elements. System identification of the recorded data has been carried out using Parametric State Space Modeling (N4SID) and the numerical models have been updated accordingly. The study demonstrates the effectiveness of base isolation systems in controlling seismic response of isolated buildings thereby leading to increased levels of seismic protection. The numerical models calibrated by relatively low level of earthquake shaking provides the starting point for modeling the non-linear response of the building when subjected to strong shaking.

Isolation, Identification and Drug Susceptibility of Bacteria from Genital Organs of Slaughter Sows (도축돈의 생식기내 세균분리 동정 및 약제함수성시험)

  • 한영도;김년수;이종오;육심용;정재용;김동훈
    • Korean Journal of Veterinary Service
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    • v.15 no.1
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    • pp.81-88
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    • 1992
  • This study was conducted to determine the microorganisms inhabitating in sow genital organs and their anti-microbial drug susceptibility During the period between February, 1991 and November 1991, 128 sow genital organs were sampled at six abattoirs. Gross pathological examination and bacterial isolation and identification were performed from the genital organ. In addition, antimicrobial drug susceptibility for the major organisms isolated were examined. 1. Among the bateria isolated from normal genital organs, E. coli(30.7%) Stahylococcus spp.(29.4%), Corynebarterium pyogenes(C. pyogenes) (14.7%), Streptococcus spp.(13.3%) were most freqently isolated, whereas the genera of Klebsiella, Actinobacillus, and Serratia were detected less freqently. 2. Among the bacteria isolated from abnormal genital organs, C. pyogenes,(37.7%), Stahylococcus spp.(30.2%), Proteus spp. (26.4%) , Pasteurella spp. (18.9%) , Steptococcus spp. (9.4%) were most freqently isolated whereas the genera of Pseudomonas, Serratia and Klebsiella were detected less freqently. 3. From sow genital organs showing lesion of endometritis and purulent endometritis C. pyogenes were most freqently isolated, the isolation rate being 67.7% and followed by Stahylococcus spp., E. coli, Proteus spp., Steptococcus spp. and Pasteurella spp. in the order. 4. Antimicrobial drug susceptibility of the major organisms showed that all the isolates were susceptible to cephalothin, ampicillin, chloramphenicol and sulfamethoxazole / trimethoprim, but resistant to penicillin and streptomycin.

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Isolation, Identification and Characterization of a Antidementia Acetylcholinesterase Inhibitor-Producing $Yarrowia$ $lipolytica$ S-3

  • Kang, Min-Gu;Yoon, Min-Ho;Choi, Young-Jun;Lee, Jong-Soo
    • Mycobiology
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    • v.40 no.1
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    • pp.42-46
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    • 2012
  • This report describes the isolation and identification of a potent acetylcholinesterase (AChE) inhibitor-producing yeasts. Of 731 species of yeast strain, the S-3 strain was selected as a potent producer of AChE inhibitor. The selected S-3 strain was investigated for its microbiological characteristics. The S-3 strain was found to be short-oval yeast that did not form an ascospore. The strain formed a pseudomycelium and grew in yeast malt medium containing 50% glucose and 10% ethanol. Finally, the S-3 strain was identified by its physiological characteristics and 26S ribosomal DNA sequences as $Yarrowia$ $lipolytica$ S-3.

Isolation and Identification of Aseptic Meningitis Virus in Pusan, 1998 (부산지역 무균성 뇌막염 원인 바이러스의 분리 및 동정 - 1998년을 중심으로)

  • 조경순;김만수;정구영;민상기;구평태;김병준;윤재득;지영미;김기순
    • Journal of Environmental Science International
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    • v.8 no.2
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    • pp.165-169
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    • 1999
  • The incidence of aseptic meningitis infection is ensuing and threatening the health of children. Enteroviruses are the major agents of aseptic meningitis and identification of virus has been a clue to diagnosis and epidemiology. The outbreak of aseptic meningitis occurred in Pusan, 1998. Patients were concentrated from April through November. Children were more susceptible than adults. Among 306 cases of specimens from stool, throat swab tested, only 7.2% were positive on virus isolation, 12 cases from stool and 10 from throat, respectively. All isolated 7 serotypes of viruses represented cytopathic effect on cultured cells. Three types of echovirus 6.25, 30 and coxsackievirus B2, B3, B4, B6 were identified by neutralizing antibody test. Isolated coxsackievirus and echovirus were observed by an electron microscope with negative staining.

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