Browse > Article
http://dx.doi.org/10.4014/jmb.1605.05074

A New Isolation and Evaluation Method for Marine-Derived Yeast spp. with Potential Applications in Industrial Biotechnology  

Zaky, Abdelrahman Saleh (Division of Food Sciences, School of Biosciences, University of Nottingham)
Greetham, Darren (Division of Food Sciences, School of Biosciences, University of Nottingham)
Louis, Edward J. (Centre for Genetic Architecture of Complex Traits, Department of Genetics, University of Leicester)
Tucker, Greg A. (Division of Food Sciences, School of Biosciences, University of Nottingham)
Du, Chenyu (Division of Food Sciences, School of Biosciences, University of Nottingham)
Publication Information
Journal of Microbiology and Biotechnology / v.26, no.11, 2016 , pp. 1891-1907 More about this Journal
Abstract
Yeasts that are present in marine environments have evolved to survive hostile environments that are characterized by high exogenous salt content, high concentrations of inhibitory compounds, and low soluble carbon and nitrogen levels. Therefore, yeasts isolated from marine environments could have interesting characteristics for industrial applications. However, the application of marine yeast in research or industry is currently very limited owing to the lack of a suitable isolation method. Current methods for isolation suffer from fungal interference and/or low number of yeast isolates. In this paper, an efficient and non-laborious isolation method has been developed and successfully isolated large numbers of yeasts without bacterial or fungal growth. The new method includes a three-cycle enrichment step followed by an isolation step and a confirmation step. Using this method, 116 marine yeast strains were isolated from 14 marine samples collected in the UK, Egypt, and the USA. These strains were further evaluated for the utilization of fermentable sugars (glucose, xylose, mannitol, and galactose) using a phenotypic microarray assay. Seventeen strains with higher sugar utilization capacity than the reference terrestrial yeast Saccharomyces cerevisiae NCYC 2592 were selected for identification by sequencing of the ITS and D1/D2 domains. These strains belonged to six species: S. cerevisiae, Candida tropicalis, Candida viswanathii, Wickerhamomyces anomalus, Candida glabrata, and Pichia kudriavzevii. The ability of these strains for improved sugar utilization using seawater-based media was confirmed and, therefore, they could potentially be utilized in fermentations using marine biomass in seawater media, particularly for the production of bioethanol and other biochemical products.
Keywords
Marine yeast; phenotypic microarray; identification; screening; fermentation; seawater;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Zaki AM, Wimalasena TT, Greetham D. 2014. Phenotypic characterisation of Saccharomyces spp. for tolerance to 1-butanol. J. Ind. Microbiol. Biotechnol. 41: 1627-1636.   DOI
2 Zaky AS, Du C. 2014. The isolation of novel marine yeasts; a new procedure. 31(Suppl. 132). New Biotechnol. DOI: 10.1016/j.nbt.2014.05.1939.   DOI
3 Zaky AS, Tucker GA, Daw ZY, Du C. 2014. Marine yeast isolation and industrial application. FEMS Yeast Res. 14: 813-825.   DOI
4 Guo F-J, Ma Y, Xu H-M, Wang X-H, Chi Z-M. 2013. A novel killer toxin produced by the marine-derived yeast Wickerhamomyces anomalus YF07b. Antonie Van Leeuwenhoek 103: 737-746.   DOI
5 Jones EBG, Suetrong S, Sakayaroj J, Bahkali A, Abdel-Wahab MA, Boekhout T, Pang KL. 2015. Classification of marine Ascomycota, Basidiomycota, Blastocladiomycota and Chytridiomycota. Fungal Diver. 73: 1-72.   DOI
6 Karsten U, Barrow KD, Nixdorf O, West JA, King RJ. 1997. Characterization of mannitol metabolism in the mangrove red alga Caloglossa leprieurii (Montagne) J.Agardh. Planta 201: 173-178.
7 Khambhaty Y, Upadhyay D, Kriplani Y, Joshi N, Mody K, Gandhi MR. 2013. Bioethanol from macroalgal biomass: utilization of marine yeast for production of the same. Bioenergy Res. 6: 188-195.   DOI
8 Kumar S, Gupta R, Kumar G, Sahoo D, Kuhad RC. 2013. Bioethanol production from Gracilaria verrucosa, a red alga, in a biorefinery approach. Bioresour. Technol. 135: 150-156.   DOI
9 Kohlmeyer J, Kohlmeyer E. 1979. Yeasts, pp. 556-606. In Kohlmeyer J, Kohlmeyer E (eds.). Marine Mycology Academic Press, New York.
10 Koop K, Carter RA, Newell RC. 1982. Mannitol-fermenting bacteria as evidence for export from kelp beds. Limnol. Oceanogr. 27: 950-954.   DOI
11 Kurtzman CP, Fell J. 2006. Yeast systematics and phylogeny- implications of molecular identification methods for studies in e cology, pp. 11-30. In Peter G, Rosa C (eds.). Biodiversity and Ecophysiology of Yeasts. Springer, Berlin-Heidelberg.
12 Kurtzman C, Piskur J. 2006. Taxonomy and phylogenetic diversity among the yeasts, pp. 29-46. In Sunnerhagen P, Piskur J (eds.). Comparative Genomics. Springer, Berlin-Heidelberg.
13 Nagahama T, Hamamoto M, Nakase T, Horikoshi K. 1999. Kluyveromyces nonfermentans sp. nov., a new yeast species isolated from the deep sea. Int. J. Syst. Bacteriol. 49: 1899-1905.   DOI
14 Kurtzman CP, Mateo RQ, Kolecka A, Theelen B, Robert V, Boekhout T. 2015. Advances in yeast systematics and phylogeny and their use as predictors of biotechnologically important metabolic pathways. FEMS Yeast Res. 15: fov050.   DOI
15 Kutty SN. 2009. Marine yeasts from the slope sediments of Arabian Sea and Bay of Bengal. PhD. Cochin University of Science and Technology, India.
16 Kutty SN, Philip R. 2008. Marine yeasts - a review. Yeast 25: 465-483.   DOI
17 Lin CSK, Luque R, Clark JH, Webb C, Du C. 2011. A seawater-based biorefining strategy for fermentative production and chemical transformations of succinic acid. Energy Environ. Sci. 4: 1471-1479.   DOI
18 Mitchell TG, White TJ, Taylor JW. 1992. Comparison of 5.8S ribosomal DNA sequences among the basidiomycetous yeast genera Cystofilobasidium, Filobasidium and Filobasidiella. J. Med. Vet. Mycol. 30: 207-218.   DOI
19 Nasr NF, Zaky AS, Daw ZY. 2010. Microbiological quality of active dry and compressed baker's yeast sold in Egypt. J. Pure Appl. Microbiol. 4: 455-462.
20 Obara N, Oki N, Okai M, Ishida M, Urano N. 2015. Development of a simple isolation method for yeast Saccharomyces cerevisiae with high fermentative activities from coastal waters. Stud. Sci. Technol. 4: 71-76.
21 Oshoma CE, Greetham D, Louis EJ, Smart KA, Phister TG, Powell C, Du C. 2015. Screening of non-Saccharomyces cerevisiae strains for tolerance to formic acid in bioethanol fermentation. PLoS One 10: e0135626.   DOI
22 Rhishipal R , Philip R. 1998. Selection of m arine yeasts for the generation of single cell protein from prawn-shell waste. Bioresour. Technol. 65: 255-256.   DOI
23 Ahearn DG, Roth FJ Jr, Meyers SP. 1968. Ecology and characterization of yeasts from aquatic regions of South Florida. Mar. Biol. 1: 291-308.   DOI
24 Bieleski RL. 1982. Sugar alcohols, pp. 158-192. In Loewus F, Tanner W (eds.). Plant Carbohydrates I. Springer, Berlin-Heidelberg.
25 Burgaud G, Arzur D, Durand L, Cambon-Bonavita M-A, Barbier G. 2010. Marine culturable yeasts in deep-sea hydrothermal vents: species richness and association with fauna. FEMS Microbiol. Ecol. 73: 121-133.
26 Ostergaard S, Olsson L, Johnston M, Nielsen J. 2000. Increasing galactose consumption by Saccharomyces cerevisiae through metabolic engineering of the GAL gene regulatory network. Nat. Biotechnol. 18: 1283-1286.   DOI
27 Pincus DH, Orenga S, Chatellier S. 2007. Yeast identification - past, present, and future methods. Med. Mycol. 45: 97-121.   DOI
28 Praphailong W, Van Gestel M, Fleet GH, Heard GM. 1997. Evaluation of the Biolog system for the identification of food and beverage yeasts. Lett. Appl. Microbiol. 24: 455-459.   DOI
29 Reed RH, Davison IR, Chudek JA, Foster R. 1985. The osmotic role of mannitol in the Phaeophyta: an appraisal. Phycologia 24: 35-47.   DOI
30 Sarkar S, Pramanik A, Mitra A, Mukherjee J. 2010. Bioprocessing data for the production of marine enzymes. Mar. Drugs 8: 1323-1372.   DOI
31 Seshadri R, Sieburth JM. 1975. Seaweeds as a reservoir of Candida yeasts in inshore waters. Mar. Biol. 30: 105-117.   DOI
32 Silvi S, Barghini P, Aquilanti A, Juarez-Jimenez B, Fenice M. 2013. Physiologic and metabolic characterization of a new marine isolate (BM39) of Pantoea sp. producing high levels of exopolysaccharide. Microb. Cell Fact. 12:10.   DOI
33 Urano N, Yamazaki M, Ueno R. 2001. Distribution of halotolerant and/or fermentative yeasts in aquatic environments. J. Tokyo Univ. Fish 87: 7.
34 Fell JW. 2001. Collection and identification of marine yeasts, pp. 347-356. Methods in Microbiology. Academic Press, Burlington.
35 Wang L, Chi Z, Wang X, Ju L, Chi Z, Guo N. 2008. Isolation and characterization of Candida membranifaciens subsp. flavinogenie W14-3, a novel riboflavin-producing marine yeast. Microbiol. Res. 163: 255-266.   DOI
36 Cadete R, Fonseca C, Rosa C. 2014. Novel yeast strains from Brazilian biodiversity: biotechnological applications in lignocellulose conversion into biofuels, pp. 255-279. In da Silva SS, Chandel AK (eds.). Biofuels in Brazil. Springer International Publishing.
37 Cavka A, Jonsson LJ. 2014. Comparison of the growth of filamentous fungi and yeasts in lignocellulose-derived media. Biocatal. Agric. Biotechnol. 3: 197-204.
38 Chi Z, Chi Z, Zhang T, Liu G, Li J, Wang X. 2009. Production, characterization and gene cloning of the extracellular enzymes from the marine-derived yeasts and their potential applications. Biotechnol. Adv. 27: 236-255.   DOI
39 Dinesh Kumar S, Karthik L, Gaurav Kumar, Bhaskara Rao KV. 2011. Biosynthesis of silver nanoparticles from marine yeast and their antimicrobial activity against multidrug resistant pathogens. Pharmacol. Online 3: 1100-1111.
40 Fell J, Statzell-Tallman A, Scorzetti G, Gutierrez M. 2011. Five new species of yeasts from fresh water and marine habitats in the Florida Everglades. Antonie Van Leeuwenhoek 99: 533-549.   DOI
41 Foschino R, Gallina S, Andrighetto C, Rossetti L, Galli A. 2004. Comparison of cultural methods for the identification and molecular investigation of yeasts from sourdoughs for Italian sweet baked products. FEMS Yeast Res. 4: 609-618.   DOI
42 Greetham D, Wimalasena T, Kerruish DWM, Brindley S, Ibbett RN, Linforth RL, et al. 2014. Development o f a phenotypic assay for characterisation of ethanologenic yeast strain sensitivity to inhibitors released from lignocellulosic feedstocks. J. Ind. Microbiol. Biotechnol. 41: 931-945.   DOI
43 Wickerham LJ. 1951. Taxonomy of yeasts. US Dept. Agric. Tech. Bull. 1029: 1-56.
44 Wenger JW, Schwartz K, Sherlock G. 2010. Bulk segregant analysis by high-throughput sequencing reveals a novel xylose utilization gene from Saccharomyces cerevisiae. PLoS Genet. 6: e1000942.   DOI
45 White T, Bruns T, Lee S, Taylor J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics, pp. 315-322. In Innis M, Gelfand D, Shinsky J, White T (eds.). PCR Protocols: A Guide to Methods and Applications. Academic Press, New York.
46 White WL, Coveny AH, Robertson J, Clements KD. 2010. Utilisation of mannitol by temperate marine herbivorous fishes. J. Exp. Mar. Biol. Ecol. 391: 50-56.   DOI
47 Wimalasena TT, Greetham D, Marvin ME, Liti G, Chandelia Y, Hart A, et al. 2014. Phenotypic characterisation of Saccharomyces spp. yeast for tolerance to stresses encountered during fermentation of lignocellulosic residues to produce bioethanol. Microb. Cell Fact. 13: 47.   DOI