• Title/Summary/Keyword: ion-cut

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Purification and Characterization of Recombinant Monellin Produced from Yeast Culture Medium (효모 배양액으로부터 재조합 모넬린의 정제와 특성 연구)

  • 김인호
    • KSBB Journal
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    • v.13 no.5
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    • pp.535-539
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    • 1998
  • The monellin, a sweet-taste protein, was expressed and secreted in Saccharomyces cerevisiae. The secreted menellin was concentrated using an ultrafiltration membrane with a nominal molecular weight cut off of 3,000 or by ammonium sulfate precipitation. The monellin was purified by G-25 gel filtration chromatography, followed by CM-Sepharose ion exchange chromatography. The purified monellin was characterized by SDS-PAGE (SDS-Polyacrylamide Gel Electrophoresis) and PHLC. The molecular weight of monellin was found to be 10,700 dalton, and its purity was over 95%.

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Ex-situ 7Li MAS NMR Study of Olivine Structured Material for Cathode of Lithium Ion Battery

  • Lee, Youngil;An, JiEun;Park, Seul-A;Song, HyeYeong
    • Journal of the Korean Magnetic Resonance Society
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    • v.18 no.2
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    • pp.63-68
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    • 2014
  • $^7Li$ nuclear magnetic resonance (NMR) spectra have been observed for $LiMPO_4$ (M = Fe, Mn) samples, as a promising cathode material of lithium ion battery. Observed $^7Li$ shifts of $LiFe_{1-x}Mn_xPO_4$ (x = 0, 0.6, 0.8, and 1) synthesized with solid-state reaction are compared with calculated $^7Li$ shift ranges based on the supertranferred hyperfine interaction of Li-O-M. Ex situ $^7Li$ NMR study of $LiFe_{0.4}Mn_{0.6}PO_4$ in different cut-off voltage for the first charge process is also performed to understand the relationship between $^7Li$ chemical shift and oxidation state of metals affected by delithiation process. The increment of oxidation state for metals makes to downfield shift of $^7Li$ by influencing the supertranferred hyperfine interaction.

EFFECT OF ION BEAM ASSISTED CLEANING ON ADHESION OF ALUMINIUM TO POLYMER SUBSTRATE OF PC AND PMMA

  • Kwon, Sik-Chol;Lee, Gun-Hwan;Lee, Chuel-Yong;Gob, Han-Bum;Lim, Jun-Seop;Goh, Sung-Jin
    • Journal of Surface Science and Engineering
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    • v.32 no.3
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    • pp.428-432
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    • 1999
  • As metallic surface has its unique lustrous appearance and optical reflectance in visible range of light, the metallization of plastic surface has been an essential drive toward weight reduction for fuel economy and decorations in transportation industry and has been put into practiced from wet chemical-electrochemial to dry vacuum process in view of an environmental effect. Electron-beam metallization was used in this work with an aim at improving the scratchproof surface hardness of plastic substrate with metallic finish character. Thin film of Al ($1000\AA$) and $SiO_2$($7000\AA$) were metallized on substrate of PC and PMMA and the films were evaluated by pencil test for surface hardness and by cross-cut tape test for adhesion. The ion beam treatment improved around twice as hard as non-treat surface. The ion beam is effect on its hardness and adhesion to surface hardened PC substrate.

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Using Gabion Systems with Vegetation Base Materials on Stability Analysis for the Forest Road Cut-slope Rehabilitation Techniques (임도비탈면의 복원을 위한 식생기반재 돌망태의 안정성 분석)

  • Park, Jae-Hyeon;Jeong, Yong-Ho;Choi, Hyung-Tae
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.12 no.2
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    • pp.106-113
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    • 2009
  • In this study, stability of the new gabion system with vegetation base materials was analysed. New gabion system with vegetation base materials is a new approach which has been developed to achieve lope stabilization and revegetation of forest road cut-slope by making the best use of advantages of gabion systems with vegetation base materials. Results from stability analysis are as follows. For the soil density, the angle of internal friction and unit weight of the rock fill was assumed to be $1.90g/cm^3$, $30^{\circ}$ and $2.30t/m^3$, respectively, the slope stability analysis showed that the new gabion system couldn't require any poles to fix it up, and could keep stable during both rainy and dry seasons. As the results of checks against overturning and sliding, the retaining wall with. the new gabion system could produce suitable factors of safety for overturning and sliding. Vegetation established on the surface of the new gabion systems indirectly can help to increase slope stability by prevention of surface erosion. Consequently, the new gabion system with vegetation base materials could achieve the desired effect on slope stabilization as much as existing gab ion system could do, and could promote rapid establishment of vegetation on cut-slopes.

Ion chromatographic determination of chlorite and chlorate in chlorinated food using a hydroxide eluent

  • Kim, Dasom;Jung, Sungjin;Lee, Gunyoung;Yun, Sang Soon;Lim, Ho Soo;Kim, Hekap
    • Analytical Science and Technology
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    • v.30 no.2
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    • pp.57-67
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    • 2017
  • This study was conducted to develop an analytical technique for determination of chlorite and chlorate concentrations in fresh-cut food and dried fish products by an ion chromatography/conductivity detection method using a hydroxide mobile phase. Deionized water was added to homogenized samples, which were then extracted by ultrasound extraction and centrifuged at high speed (8,500 rpm). Subsequently, a Sep-Pak tC18 cartridge was used to purify the supernatant. Chlorite and chlorate ions were separated using 20 mM KOH solution as the mobile phase and Dionex IonPac AS27 column as the stationary phase. Ethylenediamine was used as sample preservative and dibromoacetate was added to adjust for the disparity in extraction efficiencies between the food samples. The method detection limit) for chlorite and chlorate were estimated to be 0.2 mg/kg and 0.1 mg/kg, respectively, and the coefficient of determination ($r^2$) that denotes the linearity of their calibration curves were correspondingly measured to be 0.9973 and 0.9987. The recovery rate for each ion was 92.1 % and 96.3 %, with relative standard deviations of 7.47 % and 6.18 %, respectively. Although neither chlorite nor chlorate was detected in the food samples, the analytical technique developed in this study may potentially be used in the analysis of disinfected food products.

An Integrated Mach-Zehnder Interferometric Sensor based on Rib Waveguides (Rib 도파로 기반 집적 마흐젠더 간섭계 센서)

  • Choo, Sung-Joong;Park, Jung-Ho;Shin, Hyun-Joon
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.47 no.4
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    • pp.20-25
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    • 2010
  • An integrated Mach-Zehnder interferometric sensor operating at 632.8 nm was designed and fabricated by the technology of planar rib waveguides. Rib waveguide based on silica system ($SiO_2-SiO_xN_y-SiO_2$) was geometrically designed to have single mode operation and high sensitivity. It was structured by semiconductor fabrication processes such as thin film deposition, photolithography, and RIE (Reactive Ion Etching). With the power observation, propagation loss measurement by cut-back method showed about 4.82 dB/cm for rib waveguides. Additionally the chromium mask process for an etch stop was employed to solve the core damaging problem in patterning the sensing zone on the chip. Refractive index measurement of water/ethanol mixture with this device finally showed a sensitivity of about $\pi$/($4.04{\times}10^{-3}$).

Optimization of $Nd^{3+}$ ion co-doping in $CaAl_2O_4:\;Eu^{2+}$ blue phosphor ($CaAl_2O_4:Eu^{2+}$ 청색(靑色) 형광체(螢光體)의 $Nd^{3+}$ 도핑 최적화(最適化)에 관한 연구(硏究))

  • Bartwal, Kunwar Singh;Ryu, Ho-Jin
    • Resources Recycling
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    • v.16 no.5
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    • pp.46-50
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    • 2007
  • Blue phosphor calcium aluminate, $CaAl_2O_4:Eu^{2+}$ co-doped with $Nd^{3+}$ was prepared by solid state synthesis method. Phosphor materials with 1 mol% $Eu^{2+}$ and varying compositions of $Nd^{3+}$ show high brightness and long persistent luminescence. The synthesized phosphor materials were investigated by powder x-ray diffraction (XRD), SEM, TEM, photoluminescence excitation and emission studies. Broad band UV excited luminescence of the $CaAl_2O_4:Eu^{2+}:Nd^{3+}$ was observed in the blue region (${\lambda}_{max}=440\;nm$) due to transitions from the $4f^65d^1$ to the $4f^7$ configuration of the $Eu^{2+}$ ion. $Nd^{3+}$ ion doping in the phosphor results in long afterglow phosphorescence when the excitation light is cut off.

Deposition of Copper Film on Polytetrafluoroethylene (PTFE) Modified by 1 keV Ion Irradiation (1 keV $Ar^+$이온빔으로 개질된 polytetrafluoroethylene (PTFE) 위의 구리 박막 증착)

  • Cho, Jun-Sik;Yoon, Ki-hyun;Koh, Seok-Keun
    • Korean Journal of Materials Research
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    • v.10 no.1
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    • pp.77-82
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    • 2000
  • A surface of polytetrafluoroethylene(PTFE) was modified with changing ion doses by 1 keV $Ar^+$ ion irradiation and Cu films having thickness $5000\;{\AA}$ were deposited on the modified PTFE. The SEM study showed that the surface texture of modified PTFE was in the form of cones whose height increased depending on ion doses. Through XPS spectra, it was found that the intensity of F ls peaks decreased with ion doses by preferential sputtering of F atoms and the C-C and / or C-F chains were formed by the crosslinking in the newly unstable chains. Cu films were deposited uniformly along the filaments formed on the modified PTFE. In x-ray diffraction (XRD) spectra of deposited Cu films on modified PTFE, a preferred orientation along (111) and (200) planes was found and the peak intensity of (111) plane increased as surface roughness of modified PTFE increased. The resistivity of Cu films was changed from $2.7{\mu}{\Omega}cm$ of unmodified PTFE to $4.3{\mu}{\Omega}cm$ of modified PTFE at ion dose of $1{\times}10^{16}/\textrm{cm}^2$ and the abrupt increase of resistivity in the modified PTFE at ion dose of $1{\times}10^{17}/\textrm{cm}^2$ was due to being cut off the film which resulted from the increased surface roughness.

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Formation of $Al_O_3$Barrier in Magnetic Junctions on Different Substrates by $O_2$Plasma Etching

  • Wang, Zhen-Jun;Jeong, Won-Cheol;Yoon, Yeo-Geon;Jeong66, Chang-Wook;Joo, Seung-Ki
    • Journal of Magnetics
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    • v.6 no.3
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    • pp.90-93
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    • 2001
  • Co/$Al_O_3$/NiFe and CO/$Al_O_3$/Co tunnel junctions were fabricated by a radio frequency magnetron sputtering at room temperature with hard mask on glass and $4^{\circ}$ tilt cut Si (111) substrates. The barrier layer was formed through two steps. After the Al layer was deposited, it was oxidized in the chamber of a reactive ion etching system (RIE) with $O_2$plasma at various conditions. The dependence of the TMR value and junction resistance on the thickness of Al layer (before oxidation) and oxidation parameters were investigated. Magnetoresistance value of 7% at room temperature was obtained by optimizing the Al layer thickness and oxidation conditions. Circular shape junctions on $4^{\circ}$tilt cut Si (111) substrate showed 4% magnetoresistance. Photovoltaic energy conversion effect was observed with the cross-strip geometry junctions on Si substrate.

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